LOCUS       sequence-210935-        2445 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NMD sensor: pCMV-3XFLAG-Fluc-betaglobin (39PTC).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Baird TD, Cheng KC, Chen YC, Buehler E, Martin SE, Inglese J, Hogg 
            JR
  TITLE     ICE1 promotes the link between splicing and nonsense-mediated mRNA 
            decay.
  JOURNAL   eLife. 2018 Mar 12;7. pii: 33178. doi: 10.7554/eLife.33178.
  PUBMED    29528287
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2018-03-12"; volume: "7. pii: 33178. doi"; pages: " 
            10.7554/eLife.33178"
COMMENT     Sequence Label: pKC-4.06
FEATURES             Location/Qualifiers
     source          1..2445
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             32..97
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             116..1762
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="EDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAH
                     IEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPA
                     NDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSM
                     YTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHAR
                     DPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKI
                     QSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYG
                     LTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSG
                     YVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILL
                     QHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVF
                     VDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(179..197)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     protein_bind    1790..1814
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctcgtttagt gaaccgtcag aattaaccat ggactacaaa gaccatgacg gtgattataa
       61 agatcatgac atcgattaca aggatgacga tgacaagctt gcggccgcga attcagaaga
      121 cgccaaaaac ataaagaaag gcccggcgcc attctatccg ctggaagatg gaaccgctgg
      181 agagcaactg cataaggcta tgaagagata cgccctggtt cctggaacaa ttgcttttac
      241 agatgcacat atcgaggtgg acatcactta cgctgagtac ttcgaaatgt ccgttcggtt
      301 ggcagaagct atgaaacgat atgggctgaa tacaaatcac agaatcgtcg tatgcagtga
      361 aaactctctt caattcttta tgccggtgtt gggcgcgtta tttatcggag ttgcagttgc
      421 gcccgcgaac gacatttata atgaacgtga attgctcaac agtatgggca tttcgcagcc
      481 taccgtggtg ttcgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc aaaaaaagct
      541 cccaatcatc caaaaaatta ttatcatgga ttctaaaacg gattaccagg gatttcagtc
      601 gatgtacacg ttcgtcacat ctcatctacc tcccggtttt aatgaatacg attttgtgcc
      661 agagtccttc gatagggaca agacaattgc actgatcatg aactcctctg gatctactgg
      721 tctgcctaaa ggtgtcgctc tgcctcatag aactgcctgc gtgagattct cgcatgccag
      781 agatcctatt tttggcaatc aaatcattcc ggatactgcg attttaagtg ttgttccatt
      841 ccatcacggt tttggaatgt ttactacact cggatatttg atatgtggat ttcgagtcgt
      901 cttaatgtat agatttgaag aagagctgtt tctgaggagc cttcaggatt acaagattca
      961 aagtgcgctg ctggtgccaa ccctattctc cttcttcgcc aaaagcactc tgattgacaa
     1021 atacgattta tctaatttac acgaaattgc ttctggtggc gctcccctct ctaaggaagt
     1081 cggggaagcg gttgccaaga ggttccatct gccaggtatc aggcaaggat atgggctcac
     1141 tgagactaca tcagctattc tgattacacc cgagggggat gataaaccgg gcgcggtcgg
     1201 taaagttgtt ccattttttg aagcgaaggt tgtggatctg gataccggga aaacgctggg
     1261 cgttaatcaa agaggcgaac tgtgtgtgag aggtcctatg attatgtccg gttatgtaaa
     1321 caatccggaa gcgaccaacg ccttgattga caaggatgga tggctacatt ctggagacat
     1381 agcttactgg gacgaagacg aacacttctt catcgttgac cgcctgaagt ctctgattaa
     1441 gtacaaaggc tatcaggtgg ctcccgctga attggaatcc atcttgctcc aacaccccaa
     1501 catcttcgac gcaggtgtcg caggtcttcc cgacgatgac gccggtgaac ttcccgccgc
     1561 cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg attacgtcgc
     1621 cagtcaagta acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg acgaagtacc
     1681 gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga gagatcctca taaaggccaa
     1741 gaagggcgga aagatcgccg tggcgaattc atcgatagat ctgatatcaa caagtttgta
     1801 caaaaaagca ggctccgcgg ccgccccctt caccgtgcat ctgactcctg aggagaagtc
     1861 tgccgttact gccctgtggg gcaaggtgaa cgtggatgaa gttggtggtg aggccctggg
     1921 caggttggta tcaaggttac aagacaggtt taaggagacc aatagaaact gggcatgtgg
     1981 agacagagaa gactcttggg tttctgatag gcactgactc tctctgccta ttggtctatt
     2041 ttcccaccct taggctgctg gtggtctacc cttggaccta gaggttcttt gagtcctttg
     2101 gggatctgtc cactcctgat gctgttatgg gcaaccctaa ggtgaaggct catggcaaga
     2161 aagtgctcgg tgcctttagt gatggcctgg ctcacctgga caacctcaag ggcacctttg
     2221 ccacactgag tgagctgcac tgtgacaagc tgcacgtgga tcctgagaac ttcagggtga
     2281 gtctgatggg cacctcctgg gtttccttcc cctggctatt ctgctcaacc ttcctatcag
     2341 aaaaaaaggg gaagcgattc tagggagcag tctccatgac tgtgtgtgga gtgttgacaa
     2401 gagttcggat attttattct ctactcagaa ttgctgctcc ccctc
//
