LOCUS       sequence-21095-e         568 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 568)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG414GPD-Cerulean-ccdB
FEATURES             Location/Qualifiers
     source          1..568
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     208..229
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(287..303)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(287..303)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(392..415)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg tagcgggcga
       61 agcactgcac gccccaggtc agggtggtca cgagggtggg ccagggcacg ggcagcttgc
      121 cggtggtgca gatgaacttc agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc
      181 cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg atgggcacca
      241 ccccggtgaa cagctcctcg cccttgctca ccattttgtc ccgggggatc cactagttct
      301 agaatccgtc gaaactaagt tctggtgttt taaaactaaa aaaaagacta actataaaag
      361 tagaatttaa gaagtttaag aaatagattt acagaattac aatcaatacc taccgtcttt
      421 atatacttat tagtcaagta ggggaataat ttcagggaac tggtttcaac cttttttttc
      481 agctttttcc aaatcagaga gagcagaagg taatagaagg tgtaagaaaa tgaaatagat
      541 acatgcgtgg gtcaattgcc ttgtgtca
//
