LOCUS       sequence-210952-        3040 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH50581
FEATURES             Location/Qualifiers
     source          1..3040
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             110..901
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1072..1660
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1561..1580
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1814..1831
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2738..2756)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2824..2928
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
       61 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgagggaagc
      121 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
      181 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
      241 acacagcgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
      301 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
      361 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
      421 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
      481 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
      541 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
      601 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
      661 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
      721 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
      781 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
      841 tcagttggaa gaatttgtcc attacgtaaa aggcgagatc accaaggtag tcggcaaata
      901 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
      961 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     1021 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     1081 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     1141 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     1201 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     1261 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     1321 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     1381 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     1441 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     1501 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     1561 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     1621 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     1681 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     1741 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     1801 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     1861 gcctctcccc gcgcgttggc cgattcatta atcactctgt ggtctcagga gtcgacaaaa
     1921 tttagaacga acttaattat gatctcaaat acattgatac atatctcatc tagatctagg
     1981 ttatcattat gtaagaaagt tttgacgaat atggcacgac aaaatggcta gactcgatgt
     2041 aattggtatc tcaactcaac attatactta taccaaacat tagttagaca aaatttaaac
     2101 aactattttt tatgtatgca agagtcagca tatgtataat tgattcagaa tcgttttgac
     2161 gagttcggat gtagtagtag ccattattta atgtacatac taatcgtgaa tagtgaatat
     2221 gatgaaacat tgtatcttat tgtataaata tccataaaca catcatgaaa gacactttct
     2281 ttcacggtct gaattaatta tgatacaatt ctaatagaaa acgaattaaa ttacgttgaa
     2341 ttgtatgaaa tctaattgaa caagccaacc acgacgacga ctaacgttgc ctggattgac
     2401 tcggtttaag ttaaccacta aaaaaacgga gctgtcatgt aacacgcgga tcgagcaggt
     2461 cacagtcatg aagccatcaa agcaaaagaa ctaatccaag ggctgagatg attaattagt
     2521 ttaaaaatta gttaacacga gggaaaaggc tgtctgacag ccaggtcacg ttatctttac
     2581 ctgtggtcga aatgattcgt gtctgtcgat tttaattatt tttttgaaag gccgaaaata
     2641 aagttgtaag agataaaccc gcctatataa attcatatat tttcctctcc gctttgaata
     2701 cttgagacca cgaagtggct cttcagtgga cgaaagggcc tcgtgatacg cctattttta
     2761 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     2821 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     2881 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gcgctcacgc
     2941 aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg
     3001 gcttgttatg actgtttttt tttggggtac agtctatgcc
//
