LOCUS       sequence-210953-       13712 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13712)
  AUTHORS   Hsiao EC, Nguyen TD, Ng JK, Scott MJ, Chang WC, Zahed H, Conklin BR
  TITLE     Constitutive Gs activation using a single-construct 
            tetracycline-inducible expression system in embryonic stem cells and
            mice.
  JOURNAL   Stem Cell Res Ther. 2011 Mar 4. 2(2):11.
  PUBMED    21375737
REFERENCE   2  (bases 1 to 13712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Res Ther. 2011 Mar 4. 2(2):11."
COMMENT     Sequence Label: pRosa26 R1-ccdB-R2 RexNeo PI-SceI
FEATURES             Location/Qualifiers
     source          1..13712
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    6..1086
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     primer_bind     1190..1212
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     protein_bind    1260..1384
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1409..1414,1415..1432,1433..1439)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1493..2152
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1559..1578)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2494..2799
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2678..2697
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2840..2964)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      3721..3730
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3727..4530
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3790..3809)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4400..4419
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4690..4811
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4727..4746)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4781..4800
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    4832..9172
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     promoter        9222..9721
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9257..9280)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9654..9673
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             9748..10320
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          10397..10462
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     primer_bind     complement(10530..10547)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10536..10760
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(10867..10887)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(10867..10885)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10906..10922)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10906..10922)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10919..10941)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10930..10946
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10954..10960,10961..10978,10979..10984))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10999..11020
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11137..11154)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11308..11896)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11388..11407)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12067..12858,12859..12927))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12690..12709
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12928..13032)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(13085..13513)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13195..13216)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     13407..13426
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13640..13662
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     13654..13671
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     13655..13671
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13681..13700
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        13681..13699
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccaccgcggc aggccctccg agcgtggtgg agccgttctg tgagacagcc gggtacgagt
       61 cgtgacgctg gaaggggcaa gcgggtggtg ggcaggaatg cggtccgccc tgcagcaacc
      121 ggagggggag ggagaaggga gcggaaaagt ctccaccgga cgcggccatg gctcgggggg
      181 gggggggcag cggaggagcg cttccggccg acgtctcgtc gctgattggc ttcttttcct
      241 cccgccgtgt gtgaaaacac aaatggcgtg ttttggttgg cgtaaggcgc ctgtcagtta
      301 acggcagccg gagtgcgcag ccgccggcag cctcgctctg cccactgggt ggggcgggag
      361 gtaggtgggg tgaggcgagc tggacgtgcg ggcgcggtcg gcctctggcg gggcgggggg
      421 ggggagggag ggtcagcgaa agtagctcgc gcgcgagcgg ccgcccaccc tccccttcct
      481 ctgggggagt cgttttaccc gccgccggcc gggcctcgtc gtctgattgg ctctcggggc
      541 ccagaaaact ggcccttgcc attggctcgt gttcgtgcaa gttgagtcca tccgccggcc
      601 agcgggggcg gcgaggaggc gctcccaggt tccggccctc ccctcggccc cgcgccgcag
      661 agtctggccg cgcgcccctg cgcaacgtgg caggaagcgc gcgctggggg cggggacggg
      721 cagtagggct gagcggctgc ggggcgggtg caagcacgtt tccgacttga gttgcctcaa
      781 gaggggcgtg ctgagccaga cctccatcgc gcactccggg gagtggaggg aaggagcgag
      841 ggctcagttg ggctgttttg gaggcaggaa gcacttgctc tcccaaagtc gctctgagtt
      901 gttatcagta agggagctgc agtggagtag gcggggagaa ggccgcaccc ttctccggag
      961 gggaggggag tgttgcaata cctttctggg agttctctgc tgcctcctgg cttctgagga
     1021 ccgccctggg cctgggagaa tcccttcccc ctcttccctc gtgatctgca actccagtct
     1081 ttctagtgga tgcgttccca aaattagttt gttttaaaaa acgtattgaa gctatcccac
     1141 aaattgataa gtacttgaaa tccagcaagt atatagcatg gcctttgcag ggctggcaag
     1201 ccacgtttgg tggtggcgac catcctccaa aatcggatct ggttccgcgt tctagatcaa
     1261 caagtttgta caaaaaagct gaacgagaaa cgtaaaatga tataaatatc aatatattaa
     1321 attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac
     1381 tatggcggcc gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt
     1441 gtggattttg agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa
     1501 aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga
     1561 ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc
     1621 ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct
     1681 tgcccgcctg atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt
     1741 gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc
     1801 atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga
     1861 tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt
     1921 tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat
     1981 ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt
     2041 gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag
     2101 aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt aaagatctgg
     2161 atccggctta ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat
     2221 aagaatatat actgatatgt atacccgaag tatgtcaaaa agaggtatgc tatgaagcag
     2281 cgtattacag tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca
     2341 atatctccgg tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac
     2401 gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg
     2461 gctcttttgc tgacgagaac aggggctggt gaaatgcagt ttaaggttta cacctataaa
     2521 agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg
     2581 cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa
     2641 ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc
     2701 agtgtgccgg tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac
     2761 atcaaaaacg ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac
     2821 agccagtctg caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt
     2881 ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg
     2941 ttcagctttc ttgtacaaag tggttgatcg cgtgcatgcg acgtcatagc ttaattaagg
     3001 cgcgccgatg gaccgattcc tcccgataag taaattacag aaaagctcag gttaaatgta
     3061 agaggatctt aaatattgat ttggtttttg tcttaatggt gcccggcgta tttatacgaa
     3121 ccattaaatt tcactagacc gtttgtagtc agtggtgaac gctgcatgat ccacgctctc
     3181 aaagtgagtt gtgttgcttt gcgggaatcc agcagtcaca ttaatgctgg aaaaagttca
     3241 ggcaactagt gtactttgta gcggggtccg ggagaggctg gggtctagag tggcgatggg
     3301 acgaaagtgt aaagaaagtt ttcggtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt
     3361 gtgtaggtag gtaggtagaa actgcatcct ctgcttgtgt aaattcggtt acaagtctgt
     3421 aacagaggta ctgagatgtg actgagtctc aaggccaggc gatcgggatt ctgaagaggc
     3481 atttgcataa ctgagcaaga gtctttgccc cacccttcca cgccgaccca agacaagggg
     3541 acagctctgg gtgggtcacc tgaagggcca ggggcccgcc cacatccccg cccacaccca
     3601 ccttgagcgc ttctcattgg gcattccagc ctacccagct cggagttagt tactccgtaa
     3661 gtgtggccgg aacagagttc gtccatctaa aaagggaggg gaccggcgaa cacatctaga
     3721 gccaccatgg gatcggccat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     3781 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     3841 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     3901 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     3961 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     4021 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     4081 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     4141 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     4201 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     4261 gcgcgcatgc ccgacggcga tgatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     4321 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     4381 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     4441 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     4501 ttctatcgcc ttcttgacga gttcttctga ttcgaaatga ccgaccaagc gacgcccaac
     4561 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     4621 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     4681 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     4741 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     4801 aatgtatctt atcatgtctg tatttaatta actagaagat gggcgggagt cttctgggca
     4861 ggcttaaagg ctaacctggt gtgtgggcgt tgtcctgcag gggaattgaa caggtgtaaa
     4921 attggaggga caagacttcc cacagatttt cggttttgtc gggaagtttt ttaatagggg
     4981 caaataagga aaatgggagg ataggtagtc atctggggtt ttatgcagca aaactacagg
     5041 ttattattgc ttgtgatccg cctcggagta ttttccatcg aggtagatta aagacatgct
     5101 cacccgagtt ttatactctc ctgcttgaga tccttactac agtatgaaat tacagtgtcg
     5161 cgagttagac tatgtaagca gaattttaat catttttaaa gagcccagta cttcatatcc
     5221 atttctcccg ctccttctgc agccttatca aaaggtattt tagaacactc attttagccc
     5281 cattttcatt tattatactg gcttatccaa cccctagaca gagcattggc attttccctt
     5341 tcctgatctt agaagtctga tgactcatga aaccagacag attagttaca tacaccacaa
     5401 atcgaggctg tagctggggc ctcaacactg cagttctttt ataactcctt agtacacttt
     5461 ttgttgatcc tttgccttga tccttaattt tcagtgtcta tcacctctcc cgtcaggtgg
     5521 tgttccacat ttgggcctat tctcagtcca gggagtttta caacaataga tgtattgaga
     5581 atccaaccta aagcttaact ttccactccc atgaatgcct ctctcctttt tctccattta
     5641 taaactgagc tattaaccat taatggtttc caggtggatg tctcctcccc caatattacc
     5701 tgatgtatct tacatattgc caggctgata ttttaagaca ttaaaaggta tatttcatta
     5761 ttgagccaca tggtattgat tactgcttac taaaattttg tcattgtaca catctgtaaa
     5821 aggtggttcc ttttggaatg caaagttcag gtgtttgttg tctttcctga cctaaggtct
     5881 tgtgagcttg tattttttct atttaagcag tgctttctct tggactggct tgactcatgg
     5941 cattctacac gttattgctg gtctaaatgt gattttgcca agcttcttca ggacctataa
     6001 ttttgcttga cttgtagcca aacacaagta aaatgattaa gcaacaaatg tatttgtgaa
     6061 gcttggtttt taggttgttg tgttgtgtgt gcttgtgctc tataataata ctatccaggg
     6121 gctggagagg tggctcggag ttcaagagca cagactgctc ttccagaagt cctgagttca
     6181 attcccagca accacatggt ggctcacaac catctgtaat gggatctgat gccctcttct
     6241 ggtgtgtctg aagaccacaa gtgtattcac attaaataaa taaatcctcc ttcttcttct
     6301 tttttttttt tttaaagaga atactgtctc cagtagaatt tactgaagta atgaaatact
     6361 ttgtgtttgt tccaatatgg tagccaataa tcaaattact ctttaagcac tggaaatgtt
     6421 accaaggaac taatttttat ttgaagtgta actgtggaca gaggagccat aactgcagac
     6481 ttgtgggata cagaagacca atgcagactt taatgtcttt tctcttacac taagcaataa
     6541 agaaataaaa attgaacttc tagtatccta tttgtttaaa ctgctagctt tacttaactt
     6601 ttgtgcttca tctatacaaa gctgaaagct aagtctgcag ccattactaa acatgaaagc
     6661 aagtaatgat aattttggat ttcaaaaatg tagggccaga gtttagccag ccagtggtgg
     6721 tgcttgcctt tatgccttta atcccagcac tctggaggca gagacaggca gatctctgag
     6781 tttgagccca gcctggtcta cacatcaagt tctatctagg atagccagga atacacacag
     6841 aaaccctgtt ggggaggggg gctctgagat ttcataaaat tataattgaa gcattcccta
     6901 atgagccact atggatgtgg ctaaatccgt ctacctttct gatgagattt gggtattatt
     6961 ttttctgtct ctgctgttgg ttgggtcttt tgacactgtg ggctttcttt aaagcctcct
     7021 tcctgccatg tggtctcttg tttgctacta acttcccatg gcttaaatgg catggctttt
     7081 tgccttctaa gggcagctgc tgagatttgc agcctgattt ccagggtggg gttgggaaat
     7141 ctttcaaaca ctaaaattgt cctttaattt tttttttaaa aaatgggtta tataataaac
     7201 ctcataaaat agttatgagg agtgaggtgg actaatatta aatgagtccc tcccctataa
     7261 aagagctatt aaggcttttt gtcttatact taactttttt tttaaatgtg gtatctttag
     7321 aaccaagggt cttagagttt tagtatacag aaactgttgc atcgcttaat cagattttct
     7381 agtttcaaat ccagagaatc caaattcttc acagccaaag tcaaattaag aatttctgac
     7441 ttttaatgtt aatttgctta ctgtgaatat aaaaatgata gcttttcctg aggcagggtc
     7501 tcactatgta tctctgcctg atctgcaaca agatatgtag actaaagttc tgcctgcttt
     7561 tgtctcctga atactaaggt taaaatgtag taatactttt ggaacttgca ggtcagattc
     7621 ttttataggg gacacactaa gggagcttgg gtgatagttg gtaaaatgtg tttcaagtga
     7681 tgaaaacttg aattattatc accgcaacct actttttaaa aaaaaaagcc aggcctgtta
     7741 gagcatgctt aagggatccc taggacttgc tgagcacaca agagtagtta cttggcaggc
     7801 tcctggtgag agcatatttc aaaaaacaag gcagacaacc aagaaactac agttaaggtt
     7861 acctgtcttt aaaccatctg catatacaca gggatattaa aatattccaa ataatatttc
     7921 attcaagttt tcccccatca aattgggaca tggatttctc cggtgaatag gcagagttgg
     7981 aaactaaaca aatgttggtt ttgtgatttg tgaaattgtt ttcaagtgat agttaaagcc
     8041 catgagatac agaacaaagc tgctatttcg aggtctcttg gtttatactc agaagcactt
     8101 ctttgggttt ccctgcacta tcctgatcat gtgctaggcc taccttaggc tgattgttgt
     8161 tcaaataaac ttaagtttcc tgtcaggtga tgtcatatga tttcatatat caaggcaaaa
     8221 catgttatat atgttaaaca tttgtactta atgtgaaagt taggtctttg tgggtttgat
     8281 ttttaatttt caaaacctga gctaaataag tcatttttac atgtcttaca tttggtggaa
     8341 ttgtataatt gtggtttgca ggcaagactc tctgacctag taaccctacc tatagagcac
     8401 tttgctgggt cacaagtcta ggagtcaagc atttcacctt gaagttgaga cgttttgtta
     8461 gtgtatacta gtttatatgt tggaggacat gtttatccag aagatattca ggactatttt
     8521 tgactgggct aaggaattga ttctgattag cactgttagt gagcattgag tggcctttag
     8581 gcttgaattg gagtcacttg tatatctcaa ataatgctgg ccttttttaa aaagcccttg
     8641 ttctttatca ccctgttttc tacataattt ttgttcaaag aaatacttgt ttggatctcc
     8701 ttttgacaac aatagcatgt tttcaagcca tatttttttt cctttttttt tttttttttg
     8761 gtttttcgag acagggtttc tctgtatagc cctggctgtc ctggaactca ctttgtagac
     8821 caggctggcc tcgaactcag aaatccgcct gcctctgcct cctgagtgcc gggattaaag
     8881 gcgtgcacca ccacgcctgg ctaagttgga tattttgtta tataactata accaatacta
     8941 actccactgg gtggattttt aattcagtca gtagtcttaa gtggtcttta ttggcccttc
     9001 attaaaatct actgttcact ctaacagagg ctgttggtac tagtggcact taagcaactt
     9061 cctacggata tactagcaga ttaagggtca gggatagaaa ctagtctagc gttttgtata
     9121 cctaccagct ttatactacc ttgttctgat agaaatattt caggacatct agcttatcga
     9181 taccgtcgac ggtatcgata agcttgatat cgaattctac cgggtagggg aggcgctttt
     9241 cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag
     9301 tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt
     9361 tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc
     9421 gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca
     9481 gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca
     9541 gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct
     9601 caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg
     9661 cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc
     9721 gacctgcagg tcctcgccat ggatcctgat gatgttgttg attcttctaa atcttttgtg
     9781 atggaaaact tttcttcgta ccacgggact aaacctggtt atgtagattc cattcaaaaa
     9841 ggtatacaaa agccaaaatc tggtacacaa ggaaattatg acgatgattg gaaagggttt
     9901 tatagtaccg acaataaata cgacgctgcg ggatactctg tagataatga aaacccgctc
     9961 tctggaaaag ctggaggcgt ggtcaaagtg acgtatccag gactgacgaa ggttctcgca
    10021 ctaaaagtgg ataatgccga aactattaag aaagagttag gtttaagtct cactgaaccg
    10081 ttgatggagc aagtcggaac ggaagagttt atcaaaaggt tcggtgatgg tgcttcgcgt
    10141 gtagtgctca gccttccctt cgctgagggg agttctagcg ttgaatatat taataactgg
    10201 gaacaggcga aagcgttaag cgtagaactt gagattaatt ttgaaacccg tggaaaacgt
    10261 ggccaagatg cgatgtatga gtatatggct caagcctgtg caggaaatcg tgtcaggcga
    10321 tctctttgtg aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga
    10381 gatttaaagc tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat
    10441 tctaattgtt tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg
    10501 gaatgcagat cctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
    10561 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
    10621 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
    10681 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
    10741 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct cgaggggggg
    10801 cccggtacct gccatttcat tacctctttc tccgcacccg acatagatgg tacccagctt
    10861 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc
    10921 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
    10981 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
    11041 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
    11101 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
    11161 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
    11221 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
    11281 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
    11341 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
    11401 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
    11461 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
    11521 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
    11581 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
    11641 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
    11701 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
    11761 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
    11821 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
    11881 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
    11941 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
    12001 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
    12061 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
    12121 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
    12181 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
    12241 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
    12301 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
    12361 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
    12421 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
    12481 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
    12541 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
    12601 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
    12661 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
    12721 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
    12781 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
    12841 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    12901 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
    12961 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
    13021 aggggttccg cgcacatttc cccggaatgg cgaatggaaa ttgtaagcgt taatattttg
    13081 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
    13141 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
    13201 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
    13261 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
    13321 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
    13381 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
    13441 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
    13501 ctacagggca cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
    13561 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
    13621 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg
    13681 taatacgact cactataggg cgaattggag ct
//
