LOCUS       sequence-210955-        2973 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICSL30003
FEATURES             Location/Qualifiers
     source          1..2973
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(99..890)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(1112..1216)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1284..1302
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             1338..2045
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1483..1501)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(2142..2159)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2313..2901)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2393..2412)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
       61 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt atttgccgac taccttggtg
      121 atctcgcctt ttacgtaatg gacaaattct tccaactgat ctgcgcgcga ggccaagcga
      181 tcttcttctt gtccaagata agcctgtcta gcttcaagta tgacgggctg atactgggcc
      241 ggcaggcgct ccattgccca gtcggcagcg acatccttcg gcgcgatttt gccggttact
      301 gcgctgtacc aaatgcggga caacgtaagc actacatttc gctcatcgcc agcccagtcg
      361 ggcggcgagt tccatagcgt taaggtttca tttagcgcct caaatagatc ctgttcagga
      421 accggatcaa agagttcctc cgccgctgga cctaccaagg caacgctatg ttctcttgct
      481 tttgtcagca agatagccag atcaatgtcg atcgtggctg gctcgaagat acctgcaaga
      541 atgtcattgc gctgccattc tccaaattgc agttcgcgct tagctggata acgccacgga
      601 atgatgtcgt cgtgcacaac aatggtgact tctacagcgc ggagaatctc gctctctcca
      661 ggggaagccg aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc
      721 cttacggtca ccgtaaccag caaatcaata tcgctgtgtg gcttcaggcc gccatccact
      781 gcggagccgt acaaatgtac ggccagcaac gtcggttcga gatggcgctc gatgacgcca
      841 actacctctg atagttgagt cgatacttcg gcgatcaccg cttccctcat gatgtttaac
      901 tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctccataa catcaaacat
      961 cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg taccccaaaa
     1021 aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg
     1081 tcaaggttct ggaccagttg cgtgagcgca tactcttcct ttttcaatat tattgaagca
     1141 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     1201 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     1261 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ccactgaaga
     1321 gccactgtgg tctcaccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg
     1381 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg
     1441 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
     1501 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca
     1561 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg
     1621 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
     1681 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
     1741 cctccgacgg ccccgtaatg cagaagaaaa ccatgggctg ggaggcctcc tccgagcgga
     1801 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg
     1861 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
     1921 ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca
     1981 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
     2041 acaagaacgc ggctattaga tcaatgtgag accacgagtg attaatgaat cggccaacgc
     2101 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     2161 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     2221 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     2281 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     2341 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     2401 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     2461 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     2521 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     2581 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     2641 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     2701 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     2761 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     2821 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     2881 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     2941 tggaacgaaa actcacgtta agggattttg gtc
//
