LOCUS       sequence-210973-        1299 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid containing His and MBP tags for 5 
            repeats of the second SH3 domain from human NCK1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Li P, Banjade S, Cheng HC, Kim S, Chen B, Guo L, Llaguno M, 
            Hollingsworth JV, King DS, Banani SF, Russo PS, Jiang QX, Nixon BT, 
            Rosen MK
  TITLE     Phase transitions in the assembly of multivalent signalling 
            proteins.
  JOURNAL   Nature. 2012 Mar 7;483(7389):336-40. doi: 10.1038/nature10879.
  PUBMED    22398450
REFERENCE   2  (bases 1 to 1299)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-03-7- 7"; volume: "483"; issue: "7389"; pages:
            "336-40"
COMMENT     Sequence Label: pMAL-SH3(2)-5R
FEATURES             Location/Qualifiers
     source          1..1299
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             22..33
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1276..1296
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 aacaataaca acaacctcgg gatcgaggga aggatttcag aatttgaaaa cctgtatttt
       61 cagggccata tggacctcaa catgcccgct tatgtgaaat ttaactacat ggctgagaga
      121 gaggatgaat tatcattgat aaaggggaca aaggtgatcg tcatggagaa aagcagtgat
      181 gggtggtggc gtggtagcta caatggacaa gttggatggt tcccttcaaa ctatgtaact
      241 gaagaaggtg acagtccttt ggcgtctggt gcgggtggtt ctgaaggtgg tggttctgaa
      301 ggtggtacct ctggtgcgac cgacctcaac atgcccgctt atgtgaaatt taactacatg
      361 gctgagagag aggatgaatt atcattgata aaggggacaa aggtgatcgt catggagaaa
      421 agcagtgatg ggtggtggcg tggtagctac aatggacaag ttggatggtt cccttcaaac
      481 tatgtaactg aagaaggtga cagtcctttg gcatccggag caggaggctc cgaaggagga
      541 ggctccgagg gaggaacatc cggagcaaca catatggacc tcaacatgcc cgcttatgtg
      601 aaatttaact acatggctga gagagaggat gaattatcat tgataaaggg gacaaaggtg
      661 atcgtcatgg agaaaagcag tgatgggtgg tggcgtggta gctacaatgg acaagttgga
      721 tggttccctt caaactatgt aactgaagaa ggtgacagtc ctttggcgtc tggtgcgggt
      781 ggttctgaag gtggtggttc tgaaggtggt acctctggtg cgaccgacct caacatgccc
      841 gcttatgtga aatttaacta catggctgag agagaggatg aattatcatt gataaagggg
      901 acaaaggtga tcgtcatgga gaaaagcagt gatgggtggt ggcgtggtag ctacaatgga
      961 caagttggat ggttcccttc aaactatgta actgaagaag gtgacagtcc tttggcatcc
     1021 ggagcaggag gctccgaagg aggaggctcc gagggaggaa catccggagc aacagacctc
     1081 aacatgcccg cttatgtgaa atttaactac atggctgaga gagaggatga attatcattg
     1141 ataaagggga caaaggtgat cgtcatggag aaaagcagtg atgggtggtg gcgtggtagc
     1201 tacaatggac aagttggatg gttcccttca aactatgtaa ctgaagaagg tgacagtcct
     1261 ttgggaggcg gatccgaaaa cctgtatttt cagggccat
//
