LOCUS       sequence-210975-         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid containing a GST tag with 4 repeats of 
            the second SH3 domain from human NCK1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Li P, Banjade S, Cheng HC, Kim S, Chen B, Guo L, Llaguno M, 
            Hollingsworth JV, King DS, Banani SF, Russo PS, Jiang QX, Nixon BT, 
            Rosen MK
  TITLE     Phase transitions in the assembly of multivalent signalling 
            proteins.
  JOURNAL   Nature. 2012 Mar 7;483(7389):336-40. doi: 10.1038/nature10879.
  PUBMED    22398450
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-03-7- 7"; volume: "483"; issue: "7389"; pages:
            "336-40"
COMMENT     Sequence Label: pGEX SH3(2)-4R
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2..19
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             20..40
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tctggttccg cgtggatctg aaaacctgta ttttcagggc catatggacc tcaacatgcc
       61 cgcttatgtg aaatttaact acatggctga gagagaggat gaattatcat tgataaaggg
      121 gacaaaggtg atcgtcatgg agaaaagcag tgatgggtgg tggcgtggta gctacaatgg
      181 acaagttgga tggttccctt caaactatgt aactgaagaa ggtgacagtc ctttggccag
      241 cggcgccgga ggcagcgagg gcggcggaag cgagggcggc acgtcaggcg ccacgcatat
      301 ggacctcaac atgcccgctt atgtgaaatt taactacatg gctgagagag aggatgaatt
      361 atcattgata aaggggacaa aggtgatcgt catggagaaa agcagtgatg ggtggtggcg
      421 tggtagctac aatggacaag ttggatggtt cccttcaaac tatgtaactg aagaaggtga
      481 cagtcctttg gcgtctggtg cgggtggttc tgaaggtggt ggttctgaag gtggtacctc
      541 tggtgcgacc gacctcaaca tgcccgctta tgtgaaattt aactacatgg ctgagagaga
      601 ggatgaatta tcattgataa aggggacaaa ggtgatcgtc atggagaaaa gcagtgatgg
      661 gtggtggcgt ggtagctaca atggacaagt tggatggttc ccttcaaact atgtaactga
      721 agaaggtgac agtcctttgg catccggagc aggaggctcc gaaggaggag gctccgaggg
      781 aggaacatcc ggagcaacag acctcaacat gcccgcttat gtgaaattta actacatggc
      841 tgagagagag gatgaattat cattgataaa ggggacaaag gtgatcgtca tggagaaaag
      901 cagtgatggg tggtggcgtg gtagctacaa tggacaagtt ggatggttcc cttcaaacta
      961 tgtaactgaa gaaggtgaca gtcctttgtg aggatcc
//
