LOCUS       sequence-210983-        9264 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for expression of human TFAP4 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9264)
  AUTHORS   D'Annibale S, Kim J, Magliozzi R, Low TY, Mohammed S, Heck AJ, 
            Guardavaccaro D
  TITLE     Proteasome-dependent degradation of transcription factor activating 
            enhancer-binding protein 4 (TFAP4) controls mitotic division.
  JOURNAL   J Biol Chem. 2014 Mar 14;289(11):7730-7. doi: 
            10.1074/jbc.M114.549535. Epub 2014 Feb 5.
  PUBMED    24500709
REFERENCE   2  (bases 1 to 9264)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.549535"; journalName: "J Biol Chem"; date: 
            "2014-03-14- 14"; volume: "289"; issue: "11"; pages: "7730-7"
COMMENT     Sequence Label: pHAGE2-EF1alpha-HA-TFAP4
FEATURES             Location/Qualifiers
     source          1..9264
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      32..620
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     774..791
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1031..1052
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1067..1072,1073..1090,1091..1097)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1105..1121
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1110..1132
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1129..1145
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1129..1145
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1195..1214
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1406..2039
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2086..2211
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2708..2941
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3126..3170
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3463..3580
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3628..4806
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3859..4797
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4754..4774
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4825..4851
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             4825..4851
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    5869..6436
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5886..6438
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6030..6047)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6257..6276
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6450..7049
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6450..6469)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6946..6966
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7063..7651
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7116..7136)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7534..7545)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    7787..7820
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             7847..8027
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8074..8092)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8160..8264
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8265..8333,8334..9125)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8483..8502)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
       61 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
      121 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
      181 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
      241 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
      301 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
      361 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
      421 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      481 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      541 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      601 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
      661 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
      721 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
      781 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
      841 cgattcatta atgcagcaag ctcatggctg actaattttt tttatttatg cagaggccga
      901 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
      961 cttttgcaaa aagctccccg tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     1021 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     1081 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     1141 atgacatgat tacgaatttc acaaataaag catttttttc actgcattct agttgtggtt
     1201 tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc
     1261 aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc
     1321 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt
     1381 aaatatgtac tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat
     1441 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca
     1501 ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     1561 gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg
     1621 agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc
     1681 ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat
     1741 cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg
     1801 actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg
     1861 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     1921 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     1981 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     2041 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     2101 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     2161 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     2221 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     2281 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     2341 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     2401 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     2461 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     2521 aacaaaagta agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag
     2581 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     2641 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     2701 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     2761 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     2821 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     2881 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     2941 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     3001 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     3061 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     3121 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     3181 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     3241 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     3301 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
     3361 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatctc
     3421 gacggtatcg ccgaattcac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     3481 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     3541 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     3601 cagagatcca gtttggacta gtcgtgaggc tccggtgccc gtcagtgggc agagcgcaca
     3661 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga
     3721 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
     3781 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
     3841 tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg
     3901 ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat
     3961 cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct
     4021 tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg
     4081 tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga
     4141 tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg
     4201 cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc
     4261 acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct
     4321 caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg
     4381 gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc
     4441 cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca
     4501 cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag
     4561 taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta
     4621 ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa
     4681 gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga
     4741 tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg
     4801 tcgtgaagcg gccgcgccac catgtacccc tacgacgtgc ccgactacgc cgagtatttc
     4861 atggtgccca ctcagaaggt gccctctttg caacatttca ggaaaacaga gaaagaagtg
     4921 ataggagggc tctgtagcct tgccaacatt ccactaaccc ccgagactca gcgggaccag
     4981 gagcggcgga ttcggcggga gatcgccaac agcaacgagc ggagacgcat gcagagcatc
     5041 aacgcgggat tccagtccct caagaccctc atcccccaca cagacggaga gaagctcagc
     5101 aaggcagcca ttctccagca gacagccgag tacatcttct ccctggagca ggagaagacc
     5161 aggctcttgc agcagaacac acagctcaag cgcttcatcc aggagctgag cggctcgtcc
     5221 cccaagcgac ggcgggcaga ggacaaggac gaaggcatag gctccccgga catctgggag
     5281 gacgagaagg cggaggacct gcggcgggag atgattgagc tgcggcagca gctggacaag
     5341 gagcgctcgg tgcgcatgat gctggaggag caggtgcgct cgctggaggc ccacatgtac
     5401 ccggaaaagc tcaaggtgat tgcgcagcag gtgcagctgc agcagcagca ggaacaggtg
     5461 aggctgctgc accaggagaa gctggagcgg gaacagcagc agctgcggac ccagcttctg
     5521 ccccctccgg cccccaccca ccaccccacg gtgatcgtgc cagcaccgcc tcctcctccc
     5581 tcccaccaca tcaatgtcgt caccatgggc ccctcctcgg tcatcaactc tgtttccaca
     5641 tcccggcaaa atctggacac catcgtgcag gcaatccagc acatcgaggg cacccaggaa
     5701 aagcaggagc tggaggagga gcagcggcga gctgtcatcg tgaagcctgt ccgcagctgc
     5761 ccggaggccc ccacctctga caccgcctcc gactccgagg cctcagacag tgacgccatg
     5821 gaccagagcc gggaggagcc gtcgggggac ggggagcttc cctgaggatc cctccccccc
     5881 ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt
     5941 attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt
     6001 cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa
     6061 tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac
     6121 cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg
     6181 tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt
     6241 tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca
     6301 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt
     6361 ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg
     6421 aaaaacacga tgataatatg gccacacata tgaccgagta caagcccacg gtgcgcctcg
     6481 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc
     6541 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag
     6601 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     6661 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     6721 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     6781 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
     6841 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     6901 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     6961 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     7021 ggtgcatgac ccgcaagccc ggtgcctgaa tcgatagatc ctaatcaacc tctggattac
     7081 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     7141 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     7201 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     7261 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     7321 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     7381 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     7441 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     7501 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     7561 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     7621 agtcggatct ccctttgggc cgcctccccg cctggtacct ttaagaccaa tgacttacaa
     7681 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     7741 ctcccaacga agacaagatc acctgcagga caggcgcgca ggtaccataa cttcgtataa
     7801 tgtatgctat acgaagttat ggcgcgccct gctttttgct tgtactgggt ctctctggtt
     7861 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     7921 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     7981 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaccc gggcgattaa
     8041 ggaaagggct agatcattct tgaagacgaa agggcctcgt gatacgccta tttttatagg
     8101 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     8161 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     8221 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     8281 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     8341 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     8401 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     8461 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc
     8521 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     8581 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     8641 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     8701 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     8761 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     8821 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     8881 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     8941 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     9001 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     9061 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     9121 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     9181 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     9241 gtgagttttc gttccactga gcgt
//
