LOCUS       sequence-210985-       11338 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of IRF4-FLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11338)
  AUTHORS   Lu G, Middleton RE, Sun H, Naniong M, Ott CJ, Mitsiades CS, Wong KK,
            Bradner JE, Kaelin WG Jr
  TITLE     The myeloma drug lenalidomide promotes the cereblon-dependent 
            destruction of Ikaros proteins.
  JOURNAL   Science. 2014 Jan 17;343(6168):305-9. doi: 10.1126/science.1244917. 
            Epub 2013 Nov 29.
  PUBMED    24292623
REFERENCE   2  (bases 1 to 11338)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1244917"; journalName: "Science"; date: 
            "2014-01-17- 17"; volume: "343"; issue: "6168"; pages: "305-9"
COMMENT     Sequence Label: plenti-CAG-IRF4-FLAG-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..11338
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          27..1043
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     967..989
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1051..1070
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    1159..1183
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2537..2561)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2581..2604
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    2639..3188
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2783..2800)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3010..3029
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3191..3193,3194..3196,3197..3910)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3191..3193,3194..3196,3197..3907)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3236..3257)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3497..3516)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3844..3865
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3987..4575
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4040..4060)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4458..4469)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4578..4594)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4579..4595)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4784..5017
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5089..5210
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5126..5145)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5180..5199
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5250..5385
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5405..5424)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5406..5424)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(5447..5805)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5487..5508)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5699..5718
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5832..5936
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5938..6295
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5938..6129
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5961..5981)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6146..6281
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6330..7124
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6330..7124
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6384..6403)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6994..7013
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7312..7331)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7356..7403
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7732..8320
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8221..8240
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        8495..8798
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8800..8998
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8953..8973
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9016..9196
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9243..9368
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9865..10098
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10283..10327
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10625..10742
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        10840..11219
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc
       61 gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg
      121 tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc
      181 ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg
      241 gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct
      301 ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca
      361 gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg
      421 ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt
      481 cggtcgggct gcaacccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt
      541 cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc
      601 ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc cggggagggc tcgggggagg
      661 ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc ggcgagccgc agccattgcc
      721 ttttatggta atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tgtgcggagc
      781 cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg
      841 ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc
      901 tccctctcca gcctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca
      961 gggcggggtt cggcttctgg cgtgtgaccg gcggctctag agcctctgct aaccatgttc
     1021 atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc
     1081 attttggcaa agaattcccc tagccgcgga atctagactg ccggatcgaa ttgtcgacga
     1141 attcggatct ggccaatcac aagtttgtac aaaaaagttg gcatgaacct ggagggcggc
     1201 ggccgaggcg gagagttcgg catgagcgcg gtgagctgcg gcaacgggaa gctccgccag
     1261 tggctgatcg accagatcga cagcggcaag taccccgggc tggtgtggga gaacgaggag
     1321 aagagcatct tccgcatccc ctggaagcac gcgggcaagc aggactacaa ccgcgaggag
     1381 gacgccgcgc tcttcaaggc ttgggcactg tttaaaggaa agttccgaga aggcatcgac
     1441 aagccggacc ctcccacctg gaagacgcgc ctgcggtgcg ctttgaacaa gagcaatgac
     1501 tttgaggaac tggttgagcg gagccagctg gacatctcag acccgtacaa agtgtacagg
     1561 attgttcctg agggagccaa aaaaggagcc aagcagctca ccttggagga cccgcagatg
     1621 tccatgagcc acccctacac catgacaacg ccttaccctt cgctcccagc ccagcaggtt
     1681 cacaactaca tgatgccacc cctcgaccga agctggaggg actacgtccc ggatcagcca
     1741 cacccggaaa tcccgtacca atgtcccatg acgtttggac cccgcggcca ccactggcaa
     1801 ggcccagctt gtgaaaatgg ttgccaggtg acaggaacct tttatgcttg tgccccacct
     1861 gagtcccagg ctcccggagt ccccacagag ccaagcataa ggtctgccga agccttggcg
     1921 ttctcagact gccggctgca catctgcctg tactaccggg aaatcctcgt gaaggagctg
     1981 accacgtcca gccccgaggg ctgccggatc tcccatggac atacgtatga cgccagcaac
     2041 ctggaccagg tcctgttccc ctacccagag gacaatggcc agaggaaaaa cattgagaag
     2101 ctgctgagcc acctggagag gggcgtggtc ctctggatgg cccccgacgg gctctatgcg
     2161 aaaagactgt gccagagcag gatctactgg gacgggcccc tggcgctgtg caacgaccgg
     2221 cccaacaaac tggagagaga ccagacctgc aagctctttg acacacagca gttcttgtca
     2281 gagctgcaag cgtttgctca ccacggccgc tccctgccaa gattccaggt gactctatgc
     2341 tttggagagg agtttccaga ccctcagagg caaagaaagc tcatcacagc tcacgtagaa
     2401 cctctgctag ccagacaact atattatttt gctcaacaaa acagtggaca tttcctgagg
     2461 ggctacgatt taccagaaca catcagcaat ccagaagatt accacagatc tatccgccat
     2521 tcctctattc aagaatgccc aactttcttg tacaaagtgg tgggaggagg tggatctggc
     2581 gactacaagg acgacgatga caagtgactc gagcgggatc aattccgccc cccccctaac
     2641 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
     2701 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
     2761 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
     2821 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
     2881 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
     2941 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
     3001 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
     3061 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
     3121 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
     3181 cgataatacc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     3241 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     3301 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     3361 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
     3421 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     3481 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     3541 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     3601 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
     3661 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
     3721 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     3781 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
     3841 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     3901 gtacaagtaa agcggccgcg actctagagt cgacctgcag gcatgcaagc ttcaggtagc
     3961 cggctaacgt tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat
     4021 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     4081 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     4141 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     4201 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     4261 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     4321 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     4381 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     4441 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     4501 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     4561 gggccgcctc cccgcatcga taccgtcgac ctcgatcgag acctagaaaa acatggagca
     4621 atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag
     4681 gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag
     4741 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     4801 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     4861 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     4921 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     4981 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     5041 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     5101 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     5161 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     5221 ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     5281 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     5341 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa
     5401 ttcgccctat agtgagtcgt attagcatgt gagcaaaagg ccagcataaa tcaaaagaat
     5461 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     5521 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     5581 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     5641 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     5701 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     5761 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc
     5821 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     5881 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg
     5941 aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     6001 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     6061 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     6121 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     6181 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     6241 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg
     6301 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     6361 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     6421 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     6481 ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg
     6541 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     6601 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     6661 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     6721 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     6781 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     6841 tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     6901 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     6961 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     7021 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     7081 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     7141 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     7201 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     7261 gcgcggggat ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg
     7321 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac
     7381 gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt
     7441 cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc
     7501 ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc
     7561 tgccatagcc tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt
     7621 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     7681 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     7741 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     7801 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     7861 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     7921 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     7981 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     8041 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     8101 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     8161 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     8221 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     8281 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     8341 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     8401 tgattctgtg gataaccgta ttaccgccat gcattagtta ttaatagtaa tcaattacgg
     8461 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     8521 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     8581 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     8641 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     8701 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     8761 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     8821 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     8881 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     8941 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagcg
     9001 cgttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     9061 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     9121 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     9181 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
     9241 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
     9301 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
     9361 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     9421 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     9481 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     9541 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     9601 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     9661 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat
     9721 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     9781 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     9841 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     9901 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     9961 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
    10021 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
    10081 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
    10141 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
    10201 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
    10261 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
    10321 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
    10381 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
    10441 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
    10501 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
    10561 attagtgaac ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc
    10621 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
    10681 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
    10741 ttcgggttta ttacagggac agcagagatc cagtttggtt agtaccgggc ccgctctagc
    10801 cctagattta aatcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt
    10861 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
    10921 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
    10981 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
    11041 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
    11101 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
    11161 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
    11221 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
    11281 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg gggggggg
//
