LOCUS       sequence-210986-        9264 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for expression of human TFAP4 (E135A/S139A) in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9264)
  AUTHORS   D'Annibale S, Kim J, Magliozzi R, Low TY, Mohammed S, Heck AJ, 
            Guardavaccaro D
  TITLE     Proteasome-dependent degradation of transcription factor activating 
            enhancer-binding protein 4 (TFAP4) controls mitotic division.
  JOURNAL   J Biol Chem. 2014 Mar 14;289(11):7730-7. doi: 
            10.1074/jbc.M114.549535. Epub 2014 Feb 5.
  PUBMED    24500709
REFERENCE   2  (bases 1 to 9264)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.549535"; journalName: "J Biol Chem"; date: 
            "2014-03-14- 14"; volume: "289"; issue: "11"; pages: "7730-7"
COMMENT     Sequence Label: pHAGE2-EF1alpha-HA-TFAP4(E135A/S139A)
FEATURES             Location/Qualifiers
     source          1..9264
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      28..616
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     770..787
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1027..1048
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1063..1068,1069..1086,1087..1093)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1101..1117
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1106..1128
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1125..1141
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1125..1141
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1191..1210
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1402..2035
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2082..2207
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2704..2937
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3122..3166
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3459..3576
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3624..4802
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3855..4793
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4750..4770
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4821..4847
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             4821..4847
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    5865..6432
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5882..6434
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6026..6043)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6253..6272
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6446..7045
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6446..6465)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6942..6962
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7059..7647
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7112..7132)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7530..7541)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    7783..7816
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             7843..8023
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8070..8088)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8156..8260
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8261..8329,8330..9121)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8479..8498)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
       61 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
      121 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
      181 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
      241 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
      301 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
      361 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
      421 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
      481 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
      541 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
      601 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
      661 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
      721 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
      781 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
      841 tcattaatgc agcaagctca tggctgacta atttttttta tttatgcaga ggccgaggcc
      901 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
      961 tgcaaaaagc tccccgtggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     1021 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     1081 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     1141 catgattacg aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     1201 caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa
     1261 tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt
     1321 actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat
     1381 atgtactaca aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc
     1441 ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag
     1501 ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
     1561 cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc
     1621 tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag
     1681 catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag
     1741 cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg
     1801 gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct
     1861 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     1921 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     1981 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     2041 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     2101 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     2161 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     2221 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     2281 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     2341 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     2401 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     2461 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     2521 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
     2581 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     2641 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     2701 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     2761 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     2821 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     2881 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     2941 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     3001 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     3061 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     3121 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     3181 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     3241 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     3301 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     3361 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg
     3421 gtatcgccga attcacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
     3481 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     3541 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
     3601 gatccagttt ggactagtcg tgaggctccg gtgcccgtca gtgggcagag cgcacatcgc
     3661 ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt
     3721 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg
     3781 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg
     3841 ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt
     3901 atggcccttg cgtgccttga attacttcca cctggctgca gtacgtgatt cttgatcccg
     3961 agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc
     4021 ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga atctggtggc
     4081 accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgac
     4141 ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca
     4201 ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat
     4261 gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag
     4321 ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg
     4381 caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg
     4441 ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt gagtcaccca
     4501 cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc
     4561 gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg tctttaggtt
     4621 ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta
     4681 ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt
     4741 ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt
     4801 gaagcggccg cgccaccatg tacccctacg acgtgcccga ctacgccgag tatttcatgg
     4861 tgcccactca gaaggtgccc tctttgcaac atttcaggaa aacagagaaa gaagtgatag
     4921 gagggctctg tagccttgcc aacattccac taacccccga gactcagcgg gaccaggagc
     4981 ggcggattcg gcgggagatc gccaacagca acgagcggag acgcatgcag agcatcaacg
     5041 cgggattcca gtccctcaag accctcatcc cccacacaga cggagagaag ctcagcaagg
     5101 cagccattct ccagcagaca gccgagtaca tcttctccct ggagcaggag aagaccaggc
     5161 tcttgcagca gaacacacag ctcaagcgct tcatccagga gctgagcggc tcgtccccca
     5221 agcgacggcg ggcagaggac aaggacgcag gcataggcgc cccggacatc tgggaggacg
     5281 agaaggcgga ggacctgcgg cgggagatga ttgagctgcg gcagcagctg gacaaggagc
     5341 gctcggtgcg catgatgctg gaggagcagg tgcgctcgct ggaggcccac atgtacccgg
     5401 aaaagctcaa ggtgattgcg cagcaggtgc agctgcagca gcagcaggaa caggtgaggc
     5461 tgctgcacca ggagaagctg gagcgggaac agcagcagct gcggacccag cttctgcccc
     5521 ctccggcccc cacccaccac cccacggtga tcgtgccagc accgcctcct cctccctccc
     5581 accacatcaa tgtcgtcacc atgggcccct cctcggtcat caactctgtt tccacatccc
     5641 ggcaaaatct ggacaccatc gtgcaggcaa tccagcacat cgagggcacc caggaaaagc
     5701 aggagctgga ggaggagcag cggcgagctg tcatcgtgaa gcctgtccgc agctgcccgg
     5761 aggcccccac ctctgacacc gcctccgact ccgaggcctc agacagtgac gccatggacc
     5821 agagccggga ggagccgtcg ggggacgggg agcttccctg aggatccctc ccccccccct
     5881 aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt
     5941 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg
     6001 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc
     6061 gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt
     6121 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta
     6181 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg
     6241 gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag
     6301 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag
     6361 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
     6421 acacgatgat aatatggcca cacatatgac cgagtacaag cccacggtgc gcctcgccac
     6481 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     6541 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     6601 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     6661 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     6721 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     6781 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
     6841 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     6901 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     6961 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     7021 catgacccgc aagcccggtg cctgaatcga tagatcctaa tcaacctctg gattacaaaa
     7081 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     7141 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     7201 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     7261 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     7321 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     7381 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     7441 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     7501 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     7561 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     7621 ggatctccct ttgggccgcc tccccgcctg gtacctttaa gaccaatgac ttacaaggca
     7681 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     7741 caacgaagac aagatcacct gcaggacagg cgcgcaggta ccataacttc gtataatgta
     7801 tgctatacga agttatggcg cgccctgctt tttgcttgta ctgggtctct ctggttagac
     7861 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     7921 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     7981 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcacccgggc gattaaggaa
     8041 agggctagat cattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     8101 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     8161 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     8221 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     8281 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     8341 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     8401 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     8461 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga
     8521 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     8581 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     8641 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     8701 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     8761 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     8821 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     8881 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     8941 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     9001 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     9061 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     9121 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     9181 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     9241 gttttcgttc cactgagcgt caga
//
