LOCUS       sequence-210987-       11468 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of IRF4-FLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11468)
  AUTHORS   Lu G, Middleton RE, Sun H, Naniong M, Ott CJ, Mitsiades CS, Wong KK,
            Bradner JE, Kaelin WG Jr
  TITLE     The myeloma drug lenalidomide promotes the cereblon-dependent 
            destruction of Ikaros proteins.
  JOURNAL   Science. 2014 Jan 17;343(6168):305-9. doi: 10.1126/science.1244917. 
            Epub 2013 Nov 29.
  PUBMED    24292623
REFERENCE   2  (bases 1 to 11468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1244917"; journalName: "Science"; date: 
            "2014-01-17- 17"; volume: "343"; issue: "6168"; pages: "305-9"
COMMENT     Sequence Label: plenti-CAG-IRF4-FLAG-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..11468
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..440
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        443..718
                     /label=chicken beta-actin promoter
     intron          719..1727
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1651..1673
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1740..1759
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    1848..1872
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3226..3250)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3270..3293
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    3328..3877
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3472..3489)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3699..3718
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3880..3882,3883..3885,3886..4599)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3880..3882,3883..3885,3886..4596)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3925..3946)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4186..4205)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4533..4554
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4676..5264
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4729..4749)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5147..5158)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5267..5283)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5268..5284)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5473..5706
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5778..5899
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5815..5834)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5869..5888
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5939..6074
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6094..6113)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6095..6113)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(6136..6494)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6176..6197)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6388..6407
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6521..6625
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6627..6984
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6627..6818
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6650..6670)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6835..6970
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7019..7813
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7019..7813
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7073..7092)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7683..7702
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8001..8020)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8045..8092
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8421..9009
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8910..8929
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        9184..9487
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9489..9687
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9642..9662
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9705..9885
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9932..10057
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10554..10787
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10972..11016
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11314..11431
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tagtaccggg cccgctctag ccctagattt aaatcgatgt acgggccaga tatacgcgtt
       61 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
      121 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      181 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      241 ctttccattg acgtcaatgg gtggactatt tacggtaaac tgcccacttg gcagtacatc
      301 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      361 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      421 tagtcatcgc tattaccatg ggtcgaggtg agccccacgt tctgcttcac tctccccatc
      481 tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg
      541 atgggggcgg gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg
      601 cggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc
      661 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg
      721 agtcgctgcg ttgccttcgc cccgtgcccc gctccgcgcc gcctcgcgcc gcccgccccg
      781 gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg
      841 ctgtaattag cgcttggttt aatgacggct cgtttctttt ctgtggctgc gtgaaagcct
      901 taaagggctc cgggagggcc ctttgtgcgg gggggagcgg ctcggggggt gcgtgcgtgt
      961 gtgtgtgcgt ggggagcgcc gcgtgcggcc cgcgctgccc ggcggctgtg agcgctgcgg
     1021 gcgcggcgcg gggctttgtg cgctccgcgt gtgcgcgagg ggagcgcggc cgggggcggt
     1081 gccccgcggt gcgggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg
     1141 gggggtgagc agggggtgtg ggcgcggcgg tcgggctgta acccccccct gcacccccct
     1201 ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtgcggg gcgtggcgcg
     1261 gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg
     1321 cctcgggccg gggagggctc gggggagggg cgcggcggcc ccggagcgcc ggcggctgtc
     1381 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac
     1441 ttcctttgtc ccaaatctgg cggagccgaa atctgggagg cgccgccgca ccccctctag
     1501 cgggcgcggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt
     1561 gcgtcgccgc gccgccgtcc ccttctccat ctccagcctc ggggctgccg cagggggacg
     1621 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct
     1681 ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagtac agctcctggg
     1741 caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattcccct agccgcggaa
     1801 tctagactgc cggatcgaat tgtcgacgaa ttcggatctg gccaatcaca agtttgtaca
     1861 aaaaagttgg catgaacctg gagggcggcg gccgaggcgg agagttcggc atgagcgcgg
     1921 tgagctgcgg caacgggaag ctccgccagt ggctgatcga ccagatcgac agcggcaagt
     1981 accccgggct ggtgtgggag aacgaggaga agagcatctt ccgcatcccc tggaagcacg
     2041 cgggcaagca ggactacaac cgcgaggagg acgccgcgct cttcaaggct tgggcactgt
     2101 ttaaaggaaa gttccgagaa ggcatcgaca agccggaccc tcccacctgg aagacgcgcc
     2161 tgcggtgcgc tttgaacaag agcaatgact ttgaggaact ggttgagcgg agccagctgg
     2221 acatctcaga cccgtacaaa gtgtacagga ttgttcctga gggagccaaa aaaggagcca
     2281 agcagctcac cttggaggac ccgcagatgt ccatgagcca cccctacacc atgacaacgc
     2341 cttacccttc gctcccagcc cagcaggttc acaactacat gatgccaccc ctcgaccgaa
     2401 gctggaggga ctacgtcccg gatcagccac acccggaaat cccgtaccaa tgtcccatga
     2461 cgtttggacc ccgcggccac cactggcaag gcccagcttg tgaaaatggt tgccaggtga
     2521 caggaacctt ttatgcttgt gccccacctg agtcccaggc tcccggagtc cccacagagc
     2581 caagcataag gtctgccgaa gccttggcgt tctcagactg ccggctgcac atctgcctgt
     2641 actaccggga aatcctcgtg aaggagctga ccacgtccag ccccgagggc tgccggatct
     2701 cccatggaca tacgtatgac gccagcaacc tggaccaggt cctgttcccc tacccagagg
     2761 acaatggcca gaggaaaaac attgagaagc tgctgagcca cctggagagg ggcgtggtcc
     2821 tctggatggc ccccgacggg ctctatgcga aaagactgtg ccagagcagg atctactggg
     2881 acgggcccct ggcgctgtgc aacgaccggc ccaacaaact ggagagagac cagacctgca
     2941 agctctttga cacacagcag ttcttgtcag agctgcaagc gtttgctcac cacggccgct
     3001 ccctgccaag attccaggtg actctatgct ttggagagga gtttccagac cctcagaggc
     3061 aaagaaagct catcacagct cacgtagaac ctctgctagc cagacaacta tattattttg
     3121 ctcaacaaaa cagtggacat ttcctgaggg gctacgattt accagaacac atcagcaatc
     3181 cagaagatta ccacagatct atccgccatt cctctattca agaatgccca actttcttgt
     3241 acaaagtggt gggaggaggt ggatctggcg actacaagga cgacgatgac aagtgactcg
     3301 agcgggatca attccgcccc ccccctaacg ttactggccg aagccgcttg gaataaggcc
     3361 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg
     3421 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca
     3481 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa
     3541 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt
     3601 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt
     3661 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca
     3721 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc
     3781 ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc
     3841 acggggacgt ggttttcctt tgaaaaacac gataatacca tggtgagcaa gggcgaggag
     3901 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     3961 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     4021 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     4081 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     4141 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     4201 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     4261 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     4321 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     4381 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     4441 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     4501 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     4561 gccgggatca ctctcggcat ggacgagctg tacaagtaaa gcggccgcga ctctagagtc
     4621 gacctgcagg catgcaagct tcaggtagcc ggctaacgtt atcaagctta tcgataatca
     4681 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     4741 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     4801 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     4861 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     4921 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     4981 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     5041 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     5101 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     5161 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     5221 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc
     5281 tcgatcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg
     5341 ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc
     5401 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     5461 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     5521 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     5581 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     5641 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     5701 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     5761 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     5821 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     5881 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     5941 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     6001 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     6061 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttagcatgtg
     6121 agcaaaaggc cagcataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
     6181 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     6241 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
     6301 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     6361 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     6421 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     6481 gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     6541 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     6601 tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt
     6661 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     6721 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     6781 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     6841 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     6901 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     6961 tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat
     7021 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     7081 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     7141 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     7201 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     7261 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     7321 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     7381 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     7441 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     7501 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg
     7561 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     7621 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     7681 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     7741 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     7801 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
     7861 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     7921 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     7981 caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct
     8041 atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc
     8101 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa
     8161 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg
     8221 ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt
     8281 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     8341 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     8401 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     8461 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     8521 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     8581 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     8641 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     8701 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     8761 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     8821 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     8881 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     8941 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     9001 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     9061 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg
     9121 cattagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     9181 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     9241 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     9301 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     9361 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     9421 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     9481 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     9541 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     9601 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     9661 tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct ctctggttag
     9721 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     9781 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     9841 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
     9901 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
     9961 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
    10021 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
    10081 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
    10141 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
    10201 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
    10261 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
    10321 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
    10381 caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga tatgagggac
    10441 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
    10501 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
    10561 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
    10621 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
    10681 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
    10741 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
    10801 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
    10861 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
    10921 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
    10981 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
    11041 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
    11101 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
    11161 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
    11221 ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact gcgtgcgcca
    11281 attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
    11341 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
    11401 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
    11461 agtttggt
//
