LOCUS       sequence-211-exp        7518 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7518)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1997
REFERENCE   2  (bases 1 to 7518)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L3812
FEATURES             Location/Qualifiers
     source          1..7518
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(148..176)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     924..944
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     promoter        5263..5367
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5368..5436,5437..6228)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5586..5605)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6399..6987
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6888..6907
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7141..7158
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7275..7296
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7311..7316,7317..7334,7335..7341)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7349..7365
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7354..7376
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7373..7389
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7373..7389
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ccgcggtttt ggcgcggatc ctatcgattc
       61 gcggccgctg tacacccggg tgccctatag tgagtcgtat tggtaccggt agaaaaaatg
      121 agtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat
      181 gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa
      241 cttaccctta aatttatttg cactactgga aaactacctg ttccatgggt aagtttaaac
      301 atatatatac taactaaccc tgattattta aattttcagc caacacttgt cactactttc
      361 tgttatggtg ttcaatgctt ctcgagatac ccagatcata tgaaacagca tgactttttc
      421 aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg
      481 aactacaaga cacgtaagtt taaacagttc ggtactaact aaccatacat atttaaattt
      541 tcaggtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt
      601 attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca
      661 cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttgt aagtttaaac
      721 atgattttac taactaacta atctgattta aattttcaga acttcaaaat tagacacaac
      781 attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat
      841 ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat
      901 cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca
      961 catggcatgg acgaactata caaaagccca caagctttgg aattcgctag ccggccatac
     1021 aagtaatccg ggttctagaa gcgctgctaa gggggccctc gtcgagtcgg tcacaatcac
     1081 ctgaaactcc aaaggcagcc agtgaggaac gtgaagaaga agaaaaagag tcatctgaac
     1141 aggtttgatt ttctttctgg tcaaaaagat gaaattattg attttcagcc agatactccc
     1201 aaaactagca gcgagaagtc tgcaagtcgt tcacagtcgc ccagagaatc gcgggaagtg
     1261 agccaagagg tatgtttttc aaaaatcaat aactgatcat aatttttatt gtttggtgaa
     1321 tttaagaaaa taatattcga aaattcctct gaattatcaa gattgcagta ttaatttcga
     1381 gaaaaattga gatattcata gagctattgt aaattttctt gatttcagac tgaaacttcg
     1441 gaaaatcaag agaaaatcaa agaaaaggat gacggggatg atcagcctgg cacaccgaac
     1501 agctatagaa gccgggaaac ttcaccagct ccaaaaaggt ccaaggagac caggtttgtc
     1561 aaaagcttcc tgcgattaat tctcatttca atttttcaga gaatcagagt ctcctgaaaa
     1621 atccccggtt cgttcaagat ctcccagaag gtcttcagca cgttccccgt cacgatctcc
     1681 tagacggcgc cgagaaagaa gctcagaaag aaagcaatcc gaagagccag caccgctacc
     1741 agagaaaaag aagaaagagc cgctggatat tctacgaaca agaaccggag gagcatatat
     1801 tccacccgcc aaacttcgac ttatgcaaca acagattagt gataagcaaa gtgaacagta
     1861 tcagagaatg aattgggaaa gaatgaagaa aaagattcac ggattggtta acagagtcaa
     1921 cgcgaagaat cttgttcaaa ttgtcagaga acttcttcaa gagaatgtga ttcgttcaaa
     1981 gtgagtgaga aaatcgaagg aaaaggaaag aattaattta atttttcagg ggacttctct
     2041 gccgtgacat tattcaagct caggctttct caccaggatt ctctaacgtc tatgcagctt
     2101 tggcggcagt tatcaactcg aaattccctc atgtcggtga acttcttctc cgtcgtctga
     2161 ttgtacagtt caaaagaagt ttccgtagaa atgacagagg cgtcacggtg aacgtgatca
     2221 aattcatcgc acatttgatt aatcaacaag ttgctcacga agttcttgcg ctggaaatca
     2281 tgattctgat gcttgaagaa ccaactgatg attcagttga agtcgccatt gcgttcctga
     2341 aagagtgtgg agcaaagctt ctggagattg ctccagcagc tcttaacagt gtctacgacc
     2401 gtcttcgtgc aattctcatg gaaactgaaa gatcggaaaa tgcactggat cgacgtattc
     2461 agtatatgat tgagactgca atgcagattc gaaaggacaa atttgcggcg tatccggcgg
     2521 tcattgaaga cttggacttg attgaggagg aggatcagat catccataca cttaatttgg
     2581 aggatgcggt tgatccggaa aatgggctta atgtgttcaa actagatcca gaattcgaaa
     2641 agaacgagga ggtttatgag gagatccgta aggaaatcat tggaaacgcc gatatttcgg
     2701 atgaggatgg tggcgacgag ttggatgatg aagaagaggg tagtgatgtg gaagaggctc
     2761 cgaagaagac tacagagatt attgataata ctgatcagaa ttgactgctt tcagaaggta
     2821 ttcattttga gttttgggcc ggcaaatctg taagttgccg gttgccgaaa atttgctgaa
     2881 tttgccggaa aaaaaaattc cggaatttat ttaaaaactt tttgtaaaaa ttaaattaaa
     2941 tttgcaactt ttcagagaag tctacctgac aatgcaatca tctttggact accaagaagc
     3001 tgctcacaaa ttgctgaaaa tgaagattcc agacagcatg caggtcagcg atgttgcaaa
     3061 gaaaaatttt cgaccaaaaa aaccaaccaa tcataaaatt taaaaaaaaa ctccgttttt
     3121 ttcttttttt ttatacgaga aaaaccaaaa aaatgtattt ttgccaaatt ctaaaatact
     3181 atccccgaaa ttttcaatat tttctctttc agaacgaact ctgcgcgatg cttgtcgatt
     3241 gttgtgctca acagcgtacc tacgagcgat tctacggaat gctcatcgaa cgtttctgcc
     3301 gacttcgcct cgaataccag caatactttg aaaagctctg ccaggacacg tattccacga
     3361 ttcaccgaat tgacatcaca aaactgcgga atttggctcg ccttattgct catttgctct
     3421 cgacggatgc tattgactgg aagattttgg ccgatatgaa aatgaccgaa gaggacacaa
     3481 cttcttctgg cagaatctat attaaatata tatttaatga acttgtggag gcgatgggaa
     3541 tggttaaact tcattcgaga gttactgatc cgtgagtttc ctagagagag ttgttttcgt
     3601 attcaatttt ccctattttc agaactttgg ctcattgctt tgttggatta ttcccacgaa
     3661 ctaatccgaa cagcgcacga ttttcgatca acttcttcac aatgattgga ttgggtggtt
     3721 tgacgttgga acttcgtgaa tggctggcaa agggtctcaa gaagaagaag ggaatgctgg
     3781 atcagttgaa ggccgaatca agctcagatt catcgtcgtc ttcggattcg tcagactcgt
     3841 ctgattcttc ggattctgac gattcatccg actcgtcttc agattcctca tcttcttcag
     3901 aatcagagcc agaaccaccg aagaaaaaga agaagaagaa cagtgaagag agttccaaaa
     3961 agaaggaaaa agagaatatt ggtcgacggg atcgtggaga caagagagct gaacgtcatc
     4021 gtgatcaaag tgtggagaac aaggacaagg atcgtcgacg tcgccaggat tctgacgaaa
     4081 atcgtcggcc agaacgagga gatgaccgca aggatcggag taaagatcgt cgtcgtcaag
     4141 actcggatga tgaggatcgg aaaggtcgtg aacgtcggga agattcaggg gaaagacgtc
     4201 gcggagatcg ggatcgacgt gatcgaaaca aggatcagga ggatcaccgt gaagatcgcc
     4261 gtgaccgaag caaggatcgt gaggatcgac gtgatcgccg tcgtcatgac tctgatgatg
     4321 atcgtaaaac tcgtcgggat agaagtgaag agcgaggagg acgtcgtcgt gaagtggaat
     4381 cggatgatcg acgccgacgt cgttgaattt tcaaatttta aatactgaat atttgttttt
     4441 tttcctatta tttatttatt ctctttgtgt tttttttctt gctttctaaa aaattaattc
     4501 aatccaaatc taaacatttt tttttctctt tccgtctccc aattcgtatt ccgctcctct
     4561 catctgaaca caatgtgcaa gtttatttat cttctcgctt tcatttcatt aggacgtggg
     4621 gggaattggt ggaaggggga aacacacaaa aggatgatgg aaatgaaata aggacacaca
     4681 atatgcaaca acattcaatt cagaaatatg gaggaaggtt taaaagaaaa cataaaaata
     4741 tatagaggag gaaggaaaac tagtaaaaaa taagcaaaga aattaggcga acgatgagaa
     4801 ttgtcctcgc ttggcaaatg cgaatccgta tggagaggca cgtttggcga aggcaaatgt
     4861 tcggtatgga gatctgtaaa aatttttaag ttgaaatttg gtgttgctct tttacaaaat
     4921 tttccgattt tcgcttgaaa ttacggtgcc aggtctcgac acgtcttcca atttttcaaa
     4981 ttcaaaagag cctttaatgg gctgtagttg ctaatttctc gtttttgaaa atttttcttc
     5041 cgtttaatcg aaatttgatg tattttattt atgattttca ataaatttca aagaaactgg
     5101 tgaaaactcg gaaaattgtg aactacagta atccaatcct taaaggcgca caccttttaa
     5161 atgtccgccc caatacgata tttttttaag attcgctaga gcggcccaaa aggccttttt
     5221 tttttggcgc cgacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     5281 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     5341 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     5401 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     5461 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     5521 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     5581 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     5641 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     5701 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     5761 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     5821 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     5881 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     5941 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     6001 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     6061 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     6121 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     6181 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     6241 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     6301 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     6361 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     6421 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     6481 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     6541 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     6601 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     6661 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     6721 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     6781 cagcgtgagc attgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     6841 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     6901 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     6961 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     7021 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     7081 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     7141 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     7201 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     7261 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     7321 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     7381 agctatgacc atgattacgc caagctgtaa gtttaaacat gatcttacta actaactatt
     7441 ctcatttaaa ttttcagagc ttaaaaatgg ctgaaatcac tcacaacgat ggatacgcta
     7501 acaacttgga aatgaaat
//
