LOCUS       sequence-211016-       11164 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor vector for creation of PhiC31 attP-flanked platforms for 
            cassette exchange using CRISPR/Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11164)
  AUTHORS   Voutev R, Mann RS
  TITLE     Robust PhiC31-Mediated Genome Engineering in Drosophila melanogaster
            Using Minimal attP/attB Phage Sites.
  JOURNAL   G3 (Bethesda). 2018 Mar 9. pii: g3.118.200051. doi: 
            10.1534/g3.118.200051.
  PUBMED    29523637
REFERENCE   2  (bases 1 to 11164)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2018 Mar 9. pii: g3.118.200051. doi: 
            10.1534/g3.118.200051."
COMMENT     Sequence Label: pRVV613
FEATURES             Location/Qualifiers
     source          1..11164
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(675..693)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1145..1216,1618..1891,1966..2620,2682..2997,3201..3332,
                     3403..4017)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     promoter        6696..7148
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     7095..7116
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             7187..7981
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7241..7260)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7851..7870
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     intron          8329..8394
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8524..8544
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8816..8950
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8853..8872)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8907..8926
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        complement(8976..8994)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9015..9031)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9015..9031)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9028..9050)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9039..9055
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9063..9069,9070..9087,9088..9093))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9108..9129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9246..9263)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9417..10005)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9497..9516)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10176..10967,10968..11036))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10799..10818
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11037..11141)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccaa gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggctctaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcctt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agtattacat aacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgccattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatctggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagata gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaagat aaataaattt catttaactc gcgaatatta atgagatgcg
     3181 agtaacattt taatttgcag atggttgcca tcttgattgg cctcatcttt ttgggccaac
     3241 aactcacgca agtgggtgtg atgaatatca acggagccat cttcctcttc ctgaccaaca
     3301 tgacctttca aaacgtcttt gccacgataa atgtaagtca tgtttagaat acatttgcat
     3361 ttcaataatt tactaacttt ctaatgaatc gattcgattt aggtgttcac ctcagagctg
     3421 ccagttttta tgagggaggc ccgaagtcga ctttatcgct gtgacacata ctttctgggc
     3481 aaaacgattg ccgaattgcc gctttttctc acagtgccac tggtcttcac ggcgattgcc
     3541 tatccgatga tcggactgcg ggccggagtg ctgcacttct tcaactgcct ggcgctggtc
     3601 actctggtgg ccaatgtgtc aacgtccttc ggatatctaa tatcctgcgc cagctcctcg
     3661 acctcgatgg cgctgtctgt gggtccgccg gttatcatac cattcctgct ctttggcggc
     3721 ttcttcttga actcgggctc ggtgccagta tacctcaaat ggttgtcgta cctctcatgg
     3781 ttccgttacg ccaacgaggg tctgctgatt aaccaatggg cggacgtgga gccgggcgaa
     3841 attagctgca catcgtcgaa caccacgtgc cccagttcgg gcaaggtcat cctggagacg
     3901 cttaacttct ccgccgccga tctgccgctg gactacgtgg gtctggccat tctcatcgtg
     3961 agcttccggg tgctcgcata tctggctcta agacttcggg cccgacgcaa ggagtagccg
     4021 acatatatcc gaaataactg cttgtttttt ttttttacca ttattaccat cgtgtttact
     4081 gtttattgcc ccctcaaaaa gctaatgtaa ttatatttgt gccaataaaa acaagatatg
     4141 acctatagaa tacaagtatt tccccttcga acatccccac aagtagactt tggatttgtc
     4201 ttctaaccaa aagacttaca cacctgcata ccttacatca aaaactcgtt tatcgctaca
     4261 taaaacaccg ggatatattt tttatataca tacttttcaa atcgcgcgcc ctcttcataa
     4321 ttcacctcca ccacaccacg tttcgtagtt gctctttcgc tgtctcccac ccgctctccg
     4381 caacacattc accttttgtt cgacgacctt ggagcgactg tcgttagttc cgcgcgattc
     4441 ggttcgctca aatggttccg agtggttcat ttcgtctcaa tagaaattag taataaatat
     4501 ttgtatgtac aatttatttg ctccaatata tttgtatata tttccctcac agctatattt
     4561 attctaattt aatattatga ctttttaagg taattttttg tgacctgttc ggagtgatta
     4621 gcgttacaat ttgaactgaa agtgacatcc agtgtttgtt ccttgtgtag atgcatctca
     4681 aaaaaatggt gggcataata gtgttgttta tatatatcaa aaataacaac tataataata
     4741 agaatacatt taatttagaa aatgcttgga tttcactgga actaggctag atttaaatgc
     4801 atgccctgca ggaagcttcc taggaggcct agatctgcgg ccgcccccaa ctggggtaac
     4861 ctttgagttc tctcagttgg gggttaatta accgcgagct cgcccgggga tctaattcaa
     4921 ttagagacta attcaattag agctaattca attaggatcc aagcttatcg atttcgaacc
     4981 ctcgaccgcc ggagtataaa tagaggcgct tcgtctacgg agcgacaatt caattcaaac
     5041 aagcaaagtg aacacgtcgc taagcgaaag ctaagcaaat aaacaagcgc agctgaacaa
     5101 gctaaacaat cgggcggccg cactagagcc ggtcgccacc atgaggtctt ccaagaatgt
     5161 tatcaaggag ttcatgaggt ttaaggttcg catggaagga acggtcaatg ggcacgagtt
     5221 tgaaatagaa ggcgaaggag aggggaggcc atacgaaggc cacaataccg taaagcttaa
     5281 ggtaaccaag gggggacctt tgccatttgc ttgggatatt ttgtcaccac aatttcagta
     5341 tggaagcaag gtatatgtca agcaccctgc cgacatacca gactataaaa agctgtcatt
     5401 tcctgaagga tttaaatggg aaagggtcat gaactttgaa gacggtggcg tcgttactgt
     5461 aacccaggat tccagtttgc aggatggctg tttcatctac aaggtcaagt tcattggcgt
     5521 gaactttcct tccgatggac ctgttatgca aaagaagaca atgggctggg aagccagcac
     5581 tgagcgtttg tatcctcgtg atggcgtgtt gaaaggagag attcataagg ctctgaagct
     5641 gaaagacggt ggtcattacc tagttgaatt caaaagtatt tacatggcaa agaagcctgt
     5701 gcagctacca gggtactact atgttgactc caaactggat ataacaagcc acaacgaaga
     5761 ctatacaatc gttgagcagt atgaaagaac cgagggacgc caccatctgt tcctttagcg
     5821 gccatcgaat tcgagctcgc ccactaagcg tcgcgccact tcaacgctcg atgggagcgt
     5881 cattggtggg cggggtaacc gtcgaaatca gtgtttacgc ttccaatcgc aacaaaaaat
     5941 tcactgcaac actgaaaagc atacgaaaac gatgaagatt gtacgagaaa ccataaagta
     6001 ttttatccac aaagacacgt atagcagaaa agccaagtta actcggcgat aagttgtgta
     6061 cacaagaata aaatcggcca gattcagtgt tgtcagaaat aagaaaaccc cactatgttt
     6121 ttctttgcct tttctttctc ccagcgatca ttcatttcgt ggtgaaagaa cggggtcatt
     6181 gcacggagtt tcgactgcgg gaaagcagag ctgccgttca cttcgtctat aattagcgct
     6241 ttctattttc cccgattcgg gccgctgctg cgcttttccg cctgctgttt gtggcaagtg
     6301 tagcagcagg ctgtgcacgc agtgtggcat gcacttggct ttccaccgtt ggtatcgatt
     6361 ctctgggacg atgagtcatt cctttcgggg ccacagcata atcgttgcca gctcaccgaa
     6421 atggtgactt catttcttaa ctgccgtcaa gcatgcgatt gtacatacat acatatttat
     6481 atatgtacat atttatgtga ctatggtagg tcgatataat agcaatcaac gcaagcaaat
     6541 gtgtcagtcc tgcttacagg aacgattcta tttagtaatt ttcgttgtat aaagtaatta
     6601 tgtatgtatg taagccccat aaatctgaaa caattaggca aaaccatgcg aagctcaatt
     6661 cgaagttcct attcttcaaa aggtatagga acttcctaga atcccaaaac aaactggtta
     6721 ttgtggtagg tcatttgttt ggcagaaaga aaactcgaga aatttctctg gccgttattc
     6781 gttattctct cttttctttt tgggtctctc cctctctgca ctaatgctct ctcactctgt
     6841 cacacagtaa acggcatact gctctcgttg gttcgagaga gcgcgcctcg aatgttcgcg
     6901 aaaagagcgc cggagtataa atagaggcgc ttcgtctacg gagcgacaat tcaattcaaa
     6961 caagcaaagt gaacacgtcg ctaagcgaaa gctaagcaaa taaacaagcg cagctgaaca
     7021 agctaaacaa tctgcagtaa agtgcaagtt aaagtgaatc aattaaaagt aaccagcaac
     7081 caagtaaatc aactgcaact actgaaatct gccaagaagt aattattgaa tacaagaaga
     7141 gaactctggg ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     7201 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     7261 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     7321 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
     7381 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     7441 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     7501 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     7561 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     7621 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     7681 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
     7741 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     7801 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     7861 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     7921 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     7981 agaagttcct attcttcaaa aggtatagga acttcaccgg tgacgtcccc ccaactgaga
     8041 gaactcaaag gttaccccag ttggggggcg cgccgctagc catatgggga ttggtggcga
     8101 cgactcctgg agcccgtcag tatcggcgga attacagctg agcgccggtc gctaccatta
     8161 ccagttggtc tggtgtcaaa aataataata accgggcagg ccatgtctgc ccgtatttcg
     8221 cgtaaggaaa tccctcgagg gtacctctag aggatctttg tgaaggaacc ttacttctgt
     8281 ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa
     8341 tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca
     8401 acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt
     8461 tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact
     8521 cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt
     8581 tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca
     8641 aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatatttgat gtatagtgcc
     8701 ttgactagag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     8761 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     8821 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     8881 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     8941 tgtctggatc cactagttct agccagcttt tgttcccttt agtgagggtt aatttcgagc
     9001 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     9061 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     9121 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     9181 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     9241 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     9301 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     9361 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     9421 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     9481 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     9541 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     9601 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     9661 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     9721 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     9781 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     9841 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     9901 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     9961 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
    10021 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
    10081 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
    10141 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
    10201 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
    10261 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
    10321 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
    10381 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
    10441 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
    10501 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
    10561 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
    10621 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
    10681 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
    10741 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
    10801 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    10861 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    10921 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    10981 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    11041 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    11101 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    11161 ccac
//
