LOCUS       sequence-211045-       11104 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Epidermal Mitochondrial-tagged Florescent Calcium Sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11104)
  AUTHORS   Xu S, Chisholm AD
  TITLE     C. elegans epidermal wounding induces a mitochondrial ROS burst that
            promotes wound repair.
  JOURNAL   Dev Cell. 2014 Oct 13;31(1):48-60. doi: 
            10.1016/j.devcel.2014.08.002.
  PUBMED    25313960
REFERENCE   2  (bases 1 to 11104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2014.08"; journalName: "Dev Cell"; date: 
            "2014-10-13- 13"; volume: "31"; issue: "1"; pages: "48-60"
COMMENT     Sequence Label: pCZ829
FEATURES             Location/Qualifiers
     source          1..11104
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1523..1539)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1523..1539)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1536..1558)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1547..1563
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1571..1577,1578..1595,1596..1601))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1616..1637
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(1698..2286)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1778..1797)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      2397..2906
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2484..2503)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2690..2711
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(2948..3739,3740..3808))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3571..3590
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3809..3913)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4119..4141
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4133..4150
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4134..4150
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(5942..5953)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7987..8005
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    8832..8856
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8871..8945
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             join(8958..8963,8964..8981,8982..8984,8985..9017,9018..9020,
                     9021..9044,9045..9071,9072..9128,9129..9134,9135..9404,
                     9405..9422,9423..9857,9858..9863,9864..10307)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6f
                     /translation="GSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNKT
                     GHAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGPV
                     LLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEEL
                     FTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTY
                     GVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIEL
                     KGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSL
                     GQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYTDSEEEIREAFRVFDKDGNG
                     YISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     CDS             join(8958..8963,8964..8981,8982..8984,8985..9017,9018..9020,
                     9021..9044,9045..9071,9072..9128,9129..9134,9135..9404,
                     9405..9422,9423..9857,9858..9863,9864..10307)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6s
                     /translation="GSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNKT
                     GHAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGPV
                     LLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEEL
                     FTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTY
                     GVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIEL
                     KGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSL
                     GQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYTDSEEEIREAFRVFDKDGNG
                     YISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      9051..9060
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     9341..9362
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(9468..9489)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9729..9748)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(11027..11047)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(11027..11045)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 cagtaatcaa ttatttttcg ctcccccctc ctcgcaagtc ggcgatttct ttgaagtttt
       61 gtcaaaagaa gagacaagga cttgatagat acgctcatca gagtgagatt cattgcaaat
      121 agtgtgtaaa tcgtggagag acgagagaaa aggtgtgaga agcttagaag ataaagaata
      181 ggaagaagaa catatctcat ttcctatttt ctctaatttt tcgttcattt ttctagacat
      241 tacccatttg atggtttttc aatatggtta tcagggtact cattttggta gttttcaaag
      301 caatttggag ttttttttta aattgtcaac tacaaaaacg cagcttaaag gtccaaatat
      361 catagtataa aaattcttta aaaaattcca aattttatat gattttttga aaattggaag
      421 aatgttagtt tttgccattt ttttggaagt ttttctccgg attttatggt acttatattt
      481 caaatataaa tttttgtagg aattcgagta tgcgagagag ctatagttta ctaaaaaaaa
      541 gtggcaaaaa ctcagtaatt gccacttttt tcaaaaatgt tttgaaaaat tttactatga
      601 tatttggcca ttttggcacc atatgtacgt tactttttac gaaacactct aatttttgaa
      661 atttgagttt catgtttaat cttttgtata aaatttttat ggtccttttg tgacaggata
      721 aaaaattgca aaaaaataga aaactttgtt tcgtgaaaac acctagttta aaaattgtgg
      781 gccacttagc gattctagtc aaaaatcaga caaatgtcag ctgccgtaac tttttccgga
      841 tcagggttat caaattttaa ttttaattgt aaatgtagaa aaatgatcgg caacattttg
      901 ctaattgaaa atattttgaa aattgctata gtacatatta atctagtatt aatatagtat
      961 taatgttata atatcagtta tgtgtttgaa taactcaagc gtaagtttcc gtaaaatatc
     1021 tttaaatttt ggtaattttt tacggtttgg tataaatttt tgcatttttt gattttctac
     1081 actttttttc gatttcgcat tttccgaatt tctctgaaat aaatcgaaca atcggaaaat
     1141 tttcgagggc acccttagcc atgcagctca ggaatttgac tttatgttcc cattcaaatt
     1201 tttttaaaat taaataaaat gctaattgta agataaatta caaaaattat taaatctcat
     1261 catccttatg atgatagtgc agtgcatgaa tatatttttc caaataggat atgattcgtc
     1321 gagtagcttc acgtaatccg ttttcttcaa tcaccatttc gaactgcaat tttttttttg
     1381 gatttttgta acaatattgt cataaattgt gattttgata ttttgcgtag agcatcagaa
     1441 cctttctgct ttgaatttta atgggattaa aaattataaa aaacttgcac cctaattaaa
     1501 gcgcgatacc tggtgaccca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     1561 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     1621 tgagctaact cacattacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     1681 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     1741 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     1801 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     1861 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     1921 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     1981 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     2041 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     2101 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     2161 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     2221 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     2281 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     2341 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttacgc
     2401 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     2461 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     2521 cgctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     2581 cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc
     2641 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     2701 ttccaaactg gaacaacact caaccctatc tcgggctatt cttttgattt ataagggatt
     2761 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     2821 tttaacaaaa tattaacgtt tacaatttaa atatttgctt atacaatctt cctgtttttg
     2881 gggcttttct gattatcaac cggggtaaat caatctaaag tatatatgag taaacttggt
     2941 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     3001 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     3061 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     3121 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     3181 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     3241 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     3301 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     3361 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     3421 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     3481 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     3541 cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa
     3601 aagtgctcat cattgggaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     3661 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     3721 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     3781 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     3841 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     3901 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtagttaac aaaaaaaagc
     3961 ccgccgaagc gggctttatt accaagcgaa gcgccattcg ccattcaggc tgcgcaactg
     4021 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
     4081 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
     4141 gacggccagt tgatcagtca tccaagtggg gatcaggaag aagttgataa agtgattcaa
     4201 aaactcgagg agctcaacac agcgatgaac ccaattttga gagcaacact gacgaagaac
     4261 tatgacgcga gggtaggcac taccttgaag ctatccacct gcctgcctgc cgcctatttc
     4321 catgtgcagt aggaacgtgt gtaggtatag gcaggcatta tcgcgtctcc tttcgaacta
     4381 cctagtacct agtaggtagt ttgaagggag gcgcgataat gcctgcctat gtctacacat
     4441 gtgcctactg cgcatggaaa taggcggtag tcaggcaggt agatagcctc ataaaattca
     4501 atacttcctt ttcagaaaga atgctggaat gtattcaaag aggagtcact cttcccattg
     4561 ctcagagaat atgaagagtt gctcggcgag caaaagttca tgattggaga tcgatattca
     4621 tgggctgaca tcgctgtcgt cgagtttcta actcggtgtc agcagtgcta cgatagcttc
     4681 tatctggctc atttcgagag gctccgaact ttttgccaga cttttgagtc tcttccgcac
     4741 attcgtccct acattcaggg acgtgttgat tcatttatct aattttttaa aatggatatt
     4801 tttcgttttt tatttgtttg ttttgctgat aattcaataa agttacgcaa cgaattttag
     4861 acatttatga aaaccgaaaa tcgacaaaaa tattttaaga aaaatagcag caaaattcaa
     4921 tattttatca aattttgttt atggtaatga gaaagaaaac atacaatatt tttctaagaa
     4981 gaagaaaaat gaccagaatt ttaagatttt tctcgaatat acgaaaaatt gataaactaa
     5041 aattttcgat ttttccattt attttgaaaa aaaaactctt gacggttttc accatgcgaa
     5101 gaggtgagaa atattctgaa agttatgcat tttgaaaaaa ttgcaaaaaa atacgttaaa
     5161 attttttgat ctattgtttt tttttgaaaa ccggaattta aaatggaatt ttttttcaaa
     5221 aattaaaatt aaaatttaaa tatttctagt gtttggatag tttttcaaaa gctagattta
     5281 acccgcgaga tgtcgaccgc tagtgtagct tacagatggc tgaagacttc gcaaaaatct
     5341 tatcttagaa acccgcgagg tgtcggccgc taaaactttg taaacctgcc aacaaaatgc
     5401 ttcgcattgt ctgaacagat gaaaccttga aacaaaacat atacaattga aatcccgtaa
     5461 agtgtcgggc gcaagaactg cgattagttg tataattgga ctagctcaaa tttgaccaaa
     5521 atctcatttt tagacctcta agatgtcggc cactataact ttattcaact tgaaactgca
     5581 aaaaaactta tcaactgtat agattcaagt attatgtcaa gtattggttt tagagctaca
     5641 gtacactctg ataacaaaat ctgcagaaat acctccctgt caattcccaa aatacctccc
     5701 ctcatctcaa ttatcccgaa aatcggttag tcattgccat cagaaaatcg aattaaaaaa
     5761 taaagatgaa atatggtagc aaactcactt cgtctttgag ccaatttatc caagtccttg
     5821 taaaaagtat tcgaaaattg ttaacggata aaatgtttat tataatatca aaaacaattt
     5881 gtcagttgac cactttttga tatatttgac agaaaccggg atgaattggc tcaaagttag
     5941 ggcgcccttc tattacaggg tttctgataa caaaccggtt attaactccc aacaagggat
     6001 gatttcaatt catcatgctc aaattgaccc aaattaagtt acatgacaaa ttcatcgccc
     6061 ttttcaactc ttcttggctc atcatctgtt attctgttcc tattctgtct gcaccctata
     6121 cccctttgca tactctctcg tcattccttt cttggatagt gtcttcttct ccccagctct
     6181 gctacttcta tgacttgcgc ccgctgcttt tccgctcgtt cctctcctct ctgacgtctt
     6241 cttctgcttc ttttctcctt cctttttccc ttctcaatct tcctcttttt cccatttctg
     6301 tcaatcattc gaagaagaag aagaagaaga ccctcattca tttcattttt ttttctgtcg
     6361 gtgtgtgctg ctcggttaag agtgagctct ctattccacg tcttcttctt tttcttcttc
     6421 gattcgaatc aatcactcca ccaaaacgca ttcgtttttg ggattcaccc cgcggttcgc
     6481 aataggtttc tttttcaaat tattagcgtt ataaatagaa aaatggtgga gtttcaataa
     6541 aaatgataat ttacaaagtg tattttgatt acatgtactc aaaaaggttg aaatttttca
     6601 taccagtttt ccggaaatcc atctgatatc attatcgtat ttttcttttt aaaaatgttt
     6661 tcaaaaaaaa acaaaatata gctggttatt tggcaccctc taattaccat tttcctgtca
     6721 caccacactc ttttccttct tccacttctt tccgttttca gccgcttcca accaaaccga
     6781 tatgaaagcc gagcaacaac gatcgattcc acccggctcg gcgaccttcc cgtcgcaggt
     6841 gagactcaga aaactagaga aaccgctcaa ctaactcttg atatccgatt tcattctttt
     6901 ctcttttctt tttgttgaac ttttccatat ttccagatgc cacggccacc accaagcacc
     6961 gaacaaggaa tcacaacgga atcggagctt gcgaagaaag ctcaaatcac tccgaacgac
     7021 gttttagcac ttccgggaat cactcaaggt atcttgctcc ctttggattt cagtttaaaa
     7081 cataaattta ggattcttat gctccccatc tgcgaacatc tataacatcg agttcaccaa
     7141 gttccaaatc cgtgatctgg acactgagca agtgcttttc gagatcgcca aaccggagaa
     7201 cgatcaggag aatgatgagt cgccacagga gtcggcaaga tacgtgcgtt atagatttgc
     7261 tccaaacttt ttgaaactca aaacggtcgg agcaactgtg gaattcaaag taggagacat
     7321 cccaatccat catttccgaa tgatcgaacg tcacttcttc aaagatcgcc ttctgaagtg
     7381 ttttgacttt gaattcggat tctgtattcc gaattcacga aacaactgtg aacatatcta
     7441 tgagttccct caactctctc aacaactcag tgagtcatta ttctaaaaag tacaattcaa
     7501 aagactaatc tctttttcag tggacgacat gatcaacaat ccaaacgaga ctcgttctga
     7561 cagcttctat ttcgtcgata acaaactcgt catgcacaac aaagccgact actcatatga
     7621 tgcataaata tttaatacaa aaatgttctg gataattatt ctgtcgaata gaaaaaaaac
     7681 tccaaatgtg attaaattcc aataattcct gtctagtttg ttccttcccc ttccccttct
     7741 catgttcaat gcattcctaa gcttttcagt tccccccttg tttctatatt ttttcgctgt
     7801 cctgtcacac tcgctaaaaa cactaatcac acggaaatct gttttcaata aaaactccaa
     7861 ctttaactca ttttcaattt caactgaaag attttttcat tagagaatgt ctagaactag
     7921 gcccgggcta cgtaatacga ctcacttaag gccttgacta gagggtacca gagctcacct
     7981 agggcgtaat acgactcact atagggcgaa ttgggtacca gcatttgaaa atttgcacca
     8041 atgtattatt taattttttt tttcgaaaat ttaacgcatt ttctctctaa aaactcgaaa
     8101 tttagtgtgt tctaaacaac agtaagcata caataccttt gttcaaaatt gacgtgcttt
     8161 ctgaaccaat atggttagtt tcaaaaattt ttgtattata ggatagaaat atttggaaat
     8221 aaattttaaa accaaactta tgcctttctc ttttagtatc ccagctaggt aatattttag
     8281 tatttgccca aatccttgaa gtaaggagta tataattttt gaaaaacaat aaaactccag
     8341 ataattcata gttttttctc gaaagaaaat ttttgagatt agttattgaa cttcattttt
     8401 gaacattatt cgttgaaaaa cactcgcttt gtctttattt tcaaaaaaat tccgatttcc
     8461 ccaaccagaa aaaaaaacag atagaagaaa tttctcctta aatttcattg tccatctctc
     8521 ttggaaacac attatctatc aaatgaaaaa cgcatttttt ttcctggcag aaaaatgaaa
     8581 ttggttagat tacactggtt aggtttgaag gtgtaacttt cgctttctca gcaactttca
     8641 gtataaaagg aaacggtcac catttagaaa gacatcagtt catcaactcg aggcatgcct
     8701 gcaggtcgac tctagaggat ccccgggatt ggccaaagga cccaaaggta tgtttcgaat
     8761 gatactaaca taacatagaa cattttcagg aggacccttg gagggtacag gtagaaaaaa
     8821 agcttgatat cacaagtttg tacaaaaaag caggctccga attcgccctt atgtccgtcc
     8881 tgacgccgct gctgctgcgg ggcttgacag gctcggcccg gcggctccca gtgccgcgcg
     8941 ccaagatcca ttcgttgggt tctcatcatc atcatcatca tggtatggct agcatgactg
     9001 gtggacagca aatgggtcgg gatctgtacg acgatgacga taaggatctc gccaccatgg
     9061 tcgactcatc acgtcgtaag tggaataaga caggtcacgc agtcagagct ataggtcggc
     9121 tgagctcact cgagaacgtc tatatcaagg ccgacaagca gaagaacggc atcaaggcga
     9181 acttcaagat ccgccacaac atcgaggacg gcggcgtgca gctcgcctac cactaccagc
     9241 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcgtgc
     9301 agtccaaact ttcgaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     9361 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagggcggt accggaggga
     9421 gcatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     9481 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggtgagggc gatgccacct
     9541 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     9601 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     9661 agcagcacga cttcttcaag tccgccatgc ccgaaggcta catccaggag cgcaccatct
     9721 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     9781 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     9841 acaagctgga gtacaacctg ccggaccaac tgactgaaga gcagatcgca gaatttaaag
     9901 aggctttctc cctatttgac aaggacgggg atgggacaat aacaaccaag gagctgggga
     9961 cggtgatgcg gtctctgggg cagaacccca cagaagcaga gctgcaggac atgatcaatg
    10021 aagtagatgc cgacggtgac ggcacaatcg acttccctga gttcctgaca atgatggcaa
    10081 gaaaaatgaa atacacagac agtgaagaag aaattagaga agcgttccgt gtgtttgata
    10141 aggatggcaa tggctacatc agtgcagcag agcttcgcca cgtgatgaca aaccttggag
    10201 agaagttaac agatgaagag gttgatgaaa tgatcaggga agcagacatc gatggggatg
    10261 gtcaggtaaa ctacgaagag tttgtacaaa tgatgacagc gaagtgacgc tgtcatcaga
    10321 tcgccatctc gcgcccgtgc ctctgacttc taagtccaat tactcttcaa catccctaca
    10381 tgctctttct ccctgtgctc ccacccccta tttttgttat tatcaaaaaa cttctcttaa
    10441 tttctttgtt ttttagcttc ttttaagtca cctctaacaa tgaaattgtg tagattcaaa
    10501 aatagaatta attcgtaata aaaagtcgaa aaaaattgtg ctccctcccc ccattaataa
    10561 taattctatc ccaaaatcta cacaatgttc tgtgtacact tcttatgttt tttacttctg
    10621 ataaattttt ttgaaacatc atagaaaaaa ccgcacacaa aataccttat catatgttac
    10681 gtttcagttt atgaccgcaa tttttatttc ttcgcacgtc tgggcctctc atgacgtcaa
    10741 atcatgctca tcgtgaaaaa gttttggagt atttttggaa tttttcaatc aagtgaaagt
    10801 ttatgaaatt aattttcctg cttttgcttt ttggggtttc ccctattgtt tgtcaagatt
    10861 tcgaggacgg cgtttttctt gctaaaatca caagtattga tgagcacgat gcaagaaaga
    10921 tcggaagaag gtttgggttt gaggctcagt ggaaggtgag tagaagttga taatttgaaa
    10981 gtggagtagt gtctatgggg tttttgccga gctccagctt ttgttccctt tagtgagggt
    11041 taattgcgga tctgcccact agtgagtcgt attataagtg caagtaagat cagtgtttgt
    11101 attt
//
