LOCUS       sequence-211049-        3275 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of FGF4-NanoLuc.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Botero S, Chiaroni-Clarke R, Simon SM
  TITLE     Escherichia coli as a platform for the study of phosphoinositide 
            biology.
  JOURNAL   Sci Adv. 2019 Mar 27;5(3):eaat4872. doi: 10.1126/sciadv.aat4872. 
            eCollection 2019 Mar.
  PUBMED    30944849
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2019-03-27"; volume: "5(3):eaat4872. doi"; pages: " 
            10.1126/sciadv.aat4872. eCollection 2019 Mar"
COMMENT     Sequence Label: pClodANL-FGF4
FEATURES             Location/Qualifiers
     source          1..3275
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             205..227
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(1330..1348)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1416..1520
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1521..1589,1590..2381)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1739..1758)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(2537..3275)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
ORIGIN
        1 agcggccgca aaaaaggtac ctgccatgta tactagacct taattaacac agctaacacc
       61 acgtcgtccc tatctgctgc cctaggtcta tgagtggttg ctggataact ttacgggcat
      121 gcataaggct cgtataatat attcagggag accacaacgg tttccctcta caaataattt
      181 tgtttaactt tgagggacaa taattttgtt taactttaag aaggagatat acattactag
      241 tatcgatggt tgcactgagc ctggcacgtc tgcctgtggc cgcacaaccg aaagaagccg
      301 ccgtgcagag cggtgccggt gattacctgc tgggcattaa acgcctgcgc cgcctgtatt
      361 gcaacgtggg catcggtttc catctgcagg ccctgccgga tggtcgcatt ggtggtgccc
      421 atgcagatac ccgtgatagc ctgctggaac tgagcccggt tgaacgcggc gtggtgagca
      481 tctttggtgt ggcaagccgc ttctttgtgg ccatgagtag caaaggcaag ctgtatggca
      541 gcccgttttt caccgatgaa tgcaccttta aagaaattct gctgccgaat aattacaatg
      601 cctatgaaag ctacaaatat ccgggcatgt tcatcgccct gagcaaaaac ggcaagacca
      661 aaaaaggcaa tcgcgtgagc ccgaccatga aagtgacaca ctttctgccg cgcctgggtg
      721 catctagagg agtttttact ctggaagatt ttgttggcga ttggcgtcag accgcgggtt
      781 ataatttgga tcaagtcctg gaacagggtg gcgtaagctc tctgttccag aacctgggtg
      841 tgagcgtgac gccgattcag cgcatcgttc tgtccggcga gaacggtctg aaaattgata
      901 ttcatgtgat catcccgtac gaaggcctga gcggtgacca aatgggtcaa atcgagaaaa
      961 tctttaaagt cgtctaccca gttgacgatc accacttcaa ggttatcttg cattacggta
     1021 cgctggtgat tgatggtgtg accccgaata tgattgacta tttcggccgt ccgtatgaag
     1081 gcattgccgt ttttgacggt aaaaagatca ccgtcaccgg taccctgtgg aatggcaata
     1141 agattattga cgagcgtctg attaacccgg acggcagcct gctgttccgc gtgaccatca
     1201 acggtgtcac gggttggcgt ctgtgcgagc gcatcctggc ataataagtc gacgttaata
     1261 acaggcctgc tggtaatcgc aggccttttt attttttttt tttgctagca aattcttgaa
     1321 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     1381 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     1441 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     1501 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     1561 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     1621 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     1681 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     1741 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac
     1801 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     1861 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     1921 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     1981 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     2041 acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg
     2101 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     2161 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     2221 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     2281 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     2341 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     2401 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     2461 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     2521 cagaccccaa gatctagcgc tgcggacaca tacaaagttg cccacagatt ccgtggataa
     2581 gcaggggact aacatgtgag gcaaaacagc agggccgcgc cggtggcgtt tttccatagg
     2641 ctccgccctc ctgccagagt tcacataaac agacgctttt ccggtgcatc tgtgggagcc
     2701 gtgaggctca accatgaatc tgacagtacg ggcgaaaccc gacaggactt aaagatcccc
     2761 accgtttccg gcgggtcgct ccctcttgcg ctctcctgtt ccgaccctgc cgtttaccgg
     2821 atacctgttc cgcctttctc ccttacggga agtgtggcgc tttctcatag ctcacacact
     2881 ggtatctcgg ctcggtgtag gtcgttcgct ccaagctggg ctgtaagcaa gaactccccg
     2941 ttcagcccga ctgctgcgcc ttatccggta actgttcact tgagtccaac ccggaaaagc
     3001 acggtaaaac gccactggca gcagccattg gtaactggga gttcgcagag gatttgttta
     3061 gctaaacacg cggttgctct tgaagtgtgc gccaaagtcc ggctacactg gaaggacaga
     3121 tttggttgct gtgctctgcg aaagccagtt accacggtta agcagttccc caactgactt
     3181 aaccttcgat caaaccacct ccccaggtgg ttttttcgtt tacagggcaa aagattacgc
     3241 gcagaaaaaa aggatctcaa gaagatcctt tgatc
//
