LOCUS       sequence-211050-       10054 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a 1677-nt firefly luciferase circular RNA in Drosophila 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10054)
  AUTHORS   Huang C, Liang D, Tatomer DC, Wilusz JE
  TITLE     A length-dependent evolutionarily conserved pathway controls nuclear
            export of circular RNAs.
  JOURNAL   Genes Dev. 2018 May 17. pii: gad.314856.118. doi: 
            10.1101/gad.314856.118.
  PUBMED    29773557
REFERENCE   2  (bases 1 to 10054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2018 May 17. pii: gad.314856.118. doi: 10.1101/gad.314856.118."
COMMENT     Sequence Label: Hy_pMT dati MCS circfirefly_1677
FEATURES             Location/Qualifiers
     source          1..10054
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1..135
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        136..562
                     /label=MT promoter
                     /note="Drosophila metallothionein promoter"
     CDS             1111..2763
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    3494..3615
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4325..4342)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4496..5084)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4576..4595)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5255..6046,6047..6115))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5878..5897
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6116..6220)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6288..6306
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7702..7981
                     /label=copia promoter
                     /note="strong promoter from the Drosophila transposable 
                     element copia (Sinclair et al., 1986)"
     CDS             8027..9052
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             8027..9052
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     intron          9415..9480
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9610..9630
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
       61 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
      121 tatcatgtct ggatcaattc gttgcaggac aggatgtggt gcccgatgtg actagctctt
      181 tgctgcaggc cgtcctatcc tctggttccg ataagagacc cagaactccg gccccccacc
      241 gcccaccgcc acccccatac atatgtggta cgcaagtaag agtgcctgcg catgccccat
      301 gtgccccacc aagagctttg catcccatac aagtccccaa agtggagaac cgaaccaatt
      361 cttcgcgggc agaacaaaag cttctgcaca cgtctccact cgaatttgga gccggccggc
      421 gtgtgcaaaa gaggtgaatc gaacgaaaga cccgtgtgta aagccgcgtt tccaaaatgt
      481 ataaaaccga gagcatctgg ccaatgtgca tcagttgtgg tcagcagcaa aatcaagtga
      541 atcatctcag tgcaactaaa ggggggatct cgagcctctg ccatgactat cgtagagtaa
      601 aaagggttaa gcatttccga ccatataaag tataaatact cttgatcaga atcaatagcc
      661 gagtcgaact ggccatgtcc gcctcaacct atgaggattg aaacaagcat tcattctgaa
      721 tacatagacg cagcgcaagt ttgttgactc atattgtcac actcactcta acgcccacaa
      781 agcgccttaa cctgccacgg acaccttttt gaaattcgaa atatttcgtt atcttttcat
      841 atttttatta gttttgtaaa tttatatcga gttgcaaaaa aaactattcc acgccaacta
      901 taactcccac aaaccgccca aaaatgccac acccactctt ttaagaaatg attcaatttt
      961 gttggattaa ataagtcgtt ttccttattt caatctatat gccgaaaaca tattgaccac
     1021 gcccactctg acccctaaaa ccgcccaatt ttaaaaattc aaaaaaccct tttacgctta
     1081 acaacttttt ttgttttaca ggcaggtacc atggaagatg ccaaaaacat taagaagggc
     1141 ccagcgccat tctacccact cgaagacggg accgccggcg agcagctgca caaagccatg
     1201 aagcgctacg ccctggtgcc cggcaccatc gcctttaccg acgcacatat cgaggtggac
     1261 attacctacg ccgagtactt cgagatgagc gttcggctgg cagaagctat gaagcgctat
     1321 gggctgaata caaaccatcg gatcgtggtg tgcagcgaga atagcttgca gttcttcatg
     1381 cccgtgttgg gtgccctgtt catcggtgtg gctgtggccc cagctaacga catctacaac
     1441 gagcgcgagc tgctgaacag catgggcatc agccagccca ccgtcgtatt cgtgagcaag
     1501 aaagggctgc aaaagatcct caacgtgcaa aagaagctac cgatcataca aaagatcatc
     1561 atcatggata gcaagaccga ctaccagggc ttccaaagca tgtacacctt cgtgacttcc
     1621 catttgccac ccggcttcaa cgagtacgac ttcgtgcccg agagcttcga ccgggacaaa
     1681 accatcgccc tgatcatgaa cagtagtggc agtaccggat tgcccaaggg cgtagcccta
     1741 ccgcaccgca ccgcttgtgt ccgattcagt catgcccgcg accccatctt cggcaaccag
     1801 atcatccccg acaccgctat cctcagcgtg gtgccatttc accacggctt cggcatgttc
     1861 accacgctgg gctacttgat ctgcggcttt cgggtcgtgc tcatgtaccg cttcgaggag
     1921 gagctattct tgcgcagctt gcaagactat aagattcaat ctgccctgct ggtgcccaca
     1981 ctatttagct tcttcgctaa gagcactctc atcgacaagt acgacctaag caacttgcat
     2041 gagatcgcca gcggcggggc gccgctcagc aaggaggtag gtgaggccgt ggccaaacgc
     2101 ttccacctac caggcatccg ccagggctac ggcctgacag aaacaaccag cgccattctg
     2161 atcacccccg aaggggacga caagcctggc gcagtaggca aggtggtgcc cttcttcgag
     2221 gctaaggtgg tggacttgga caccggtaag acactgggtg tgaaccagcg cggcgagctg
     2281 tgcgtccgtg gccccatgat catgagcggc tacgttaaca accccgaggc tacaaacgct
     2341 ctcatcgaca aggacggctg gctgcacagc ggcgacatcg cctactggga cgaggacgag
     2401 cacttcttca tcgtggaccg gctgaagagc ctgatcaaat acaagggcta ccaggtagcc
     2461 ccagccgaac tggagagcat cctgctgcaa caccccaaca tcttcgacgc cggggtcgcc
     2521 ggcctgcccg acgacgatgc cggcgagctg cccgccgcag tcgtcgtgct ggaacacggt
     2581 aaaaccatga ccgagaagga gatcgtggac tatgtggcca gccaggttac aaccgccaag
     2641 aagctgcgcg gtggtgttgt gttcgtggac gaggtgccta aaggactgac cggcaagttg
     2701 gacgcccgca agatccgcga gattctcatt aaggccaaga agggcggcaa gatcgccgtg
     2761 taacccgggg agctccaggt attgttttga agatttttct gcttatctat atactttaaa
     2821 gggaacctgg taatcgaata ttcgatgcag tccaaacaag agtagtaaaa aatcaacaga
     2881 gaacgctatt cgagttttta gactagacta tatgtacacc cgatatttag ctgttacgag
     2941 cgcaacaatg aactttcaaa attgtgtgac tgttttggac gttttgcagg tgtaagtagg
     3001 ggcgtggcca aagtgttttt ggtatgtcaa taaaaattgg caagacaatg aaaacgaaga
     3061 ttaatcaaaa cattttttgt gggggcgtgg caaacagttt tttggcaaat cgattttatt
     3121 ttatcgatga aataatatga atacattttg caagagcgtg ggagtggcag ttctggacgc
     3181 tttgtgggcg ttagaggatg cgtagcaaca aacttgcgct gcgatctatg cttaatctca
     3241 actttctagc ttttagttag atagtttctg aaatctcgac gttcatacag gcggatatgg
     3301 ccagttcgac tcgtcttttg atgctaatca agaatatatg tagtttatat ggtcggaaac
     3361 gcttccttct acctgttaca tacctttaaa caaatctaat atacgctttc cctctacgag
     3421 taatgggtat gaaaacaaaa atcaacacgc atcaacgcat tttggaaagt tgaaggcgtg
     3481 atagtgcggc cgcaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     3541 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     3601 catcaatgta tcttaactag tggatccact aggggccgcc gacgcgaggc tggatggcct
     3661 tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg caggccatgc
     3721 tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcgctc gcggctctta
     3781 ccagcctaac ttcgatcatt ggaccgctga tcgtcacggc gatttatgcc gcctcggcga
     3841 gcacatggaa cgggttggca tggattgtag gcgccgccct ataccttgtc tgcctccccg
     3901 cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg aatggaagcc ggcggcacct
     3961 cgctaacgga ttcaccactc caagaattgg agccaatcaa ttcttgcgga gaactgtgaa
     4021 tgcgcaaacc aacccttggc agaacatatc catcgcgtcc gccatctcca gcagccgcac
     4081 gcggcgcatc tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc
     4141 gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg aatcaccgat
     4201 acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag caacaacatg
     4261 aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag cgctcttccg
     4321 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     4381 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     4441 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     4501 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     4561 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     4621 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     4681 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     4741 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     4801 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     4861 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     4921 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     4981 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     5041 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     5101 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     5161 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     5221 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     5281 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     5341 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     5401 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     5461 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     5521 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg
     5581 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     5641 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     5701 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     5761 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     5821 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata
     5881 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     5941 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     6001 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     6061 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     6121 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     6181 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     6241 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     6301 cgaggccctt tcgtcttcaa gaactcacat ttgtacgaat ttttttttta tcaaaagttc
     6361 gagtttttca ccaatttcct catcaaccga gcaaggcaaa cggctttgaa taatatggtg
     6421 ttatatatac atatatcaaa tcgctgctga ctgcgtgatt gatggcccca agattacata
     6481 ttatcgaatc aggattcaga aggagatcaa tgtcaaatgc ggacaggaac atgaaagacg
     6541 cctgttatgc gcaattaaaa atttgggttt aattgctgtg gaaactgttg ttggcggcat
     6601 cttaagttcc tgtttaacaa catcaactac ttatgtacgt agaagcgttt aagccatttg
     6661 catacagatg agaactggct tttgtgctaa tcagtcaaga tgactccgat gatgatgact
     6721 cattacctga ccagttttcg ctgctttctt ttcaacaact acttgtatat gtattgtatc
     6781 caatagcaat acattgaatt tccatggtct agtcacgtat tatcatttaa ttgacaccaa
     6841 gtcgtgttat tgttgagcta tcgagttcag ctcaaacatt tcttattccc atgaataagc
     6901 cggcaaaaat atgcaatcta tgaaagttaa tataagcaaa ccttactttg actcaatacc
     6961 aatgcacttt gtgtcgatag gttcacgcaa ttgaggcgat tattccgata acccaagcga
     7021 ttgactgttc ccgtttcgat tccaattgaa atttggaaat gtacaatagt tttgctatat
     7081 gctgtcaagt acgctcttat cttctctggg ttttcttcag agtttcgaaa cgcttcttct
     7141 tttttttgtt tttttttttt tggaatctcg tattttggaa ggggctcccc tctggaattt
     7201 gttacactgt cgttatcatt gcgaacaagc ggcccgaagc tatcagcgac tttaacattt
     7261 acaatgcact tttttacgac caattaaatg tacattttcc tttcttcgcc cgttgataag
     7321 cgaacgcgat gtggcgcagg caatgtgttg ctcttgcgac acaaacgcaa tcaaaatgga
     7381 ttcaatttcg ctttttccca gtgaaacgaa gaacgaaccg accatcatga tatgctcctc
     7441 tgcatgttgc gtattgaatc aatgacaatt tcaattaagc cgcccgttcg tcatgcgttt
     7501 tcgtgcgctt cgaaatgctg ataacgctgc tgtcctccaa ctgctttgca tgtggacaca
     7561 attccattta tttaattctt ttatttggat cggttaaatt aaaaagcgcc ttgttacgca
     7621 tttaacgttg tttccggtgc gtggtggttt catgcttctg ggaacggcaa atgggtttag
     7681 gattgggaac ccctcatcat ctgttggaat atactattca acctacaaaa gtaacgttaa
     7741 acaacactac tttatatttg atatgaatgg ccacaccttt tatgccataa aacatattgt
     7801 aagagaatac cactcttttt attccttctt tccttcttgt acgttttttg ctgtaagtag
     7861 gtcgtggtgc tggtgttgca gttgaaataa cttaaaatat aaatcataaa actcaaacat
     7921 aaacttgact atttatttat ttattaagaa aggaaatata aattataaat tacaacaggt
     7981 tatggacctg cagccaagct tggcgctcgt ccgggggcaa tgagatatga aaaagcctga
     8041 actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct
     8101 gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg
     8161 atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg
     8221 gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga
     8281 gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga
     8341 aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc
     8401 cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac
     8461 tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt
     8521 gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc
     8581 cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct
     8641 gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc
     8701 ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca
     8761 gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta
     8821 tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga
     8881 tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg
     8941 gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact
     9001 cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat agagtagatg
     9061 ccgaccgaac aagagctgat ttcgagaacg cctcagccag caactcgcgc gagcctagca
     9121 aggcaaatgc gagagaacgg ccttacgctt ggtggcacag ttctcgtcca cagttcgcta
     9181 agctcgctcg gctgggtcgc gggagggccg gtcgcagtga ttcaggccct tctggattgt
     9241 gttggtcccc agggcacgat tgtcatgccc acgcactcgg gtgatctgac tgatcccgca
     9301 gattggagat cgccgcccgt gcctgccgat tgggtgcaga tctttgtgaa ggaaccttac
     9361 ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat
     9421 ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag
     9481 attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac
     9541 ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat
     9601 tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg
     9661 ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac
     9721 accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc
     9781 tttataagta ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg
     9841 catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt
     9901 tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag
     9961 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
    10021 cctgaaacat aaaatgaatg caattgttgt tgtt
//
