LOCUS       sequence-211067-        9277 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a 900-nt firefly luciferase circular RNA in Drosophila 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9277)
  AUTHORS   Huang C, Liang D, Tatomer DC, Wilusz JE
  TITLE     A length-dependent evolutionarily conserved pathway controls nuclear
            export of circular RNAs.
  JOURNAL   Genes Dev. 2018 May 17. pii: gad.314856.118. doi: 
            10.1101/gad.314856.118.
  PUBMED    29773557
REFERENCE   2  (bases 1 to 9277)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2018 May 17. pii: gad.314856.118. doi: 10.1101/gad.314856.118."
COMMENT     Sequence Label: Hy_pMT dati MCS circfirefly_900
FEATURES             Location/Qualifiers
     source          1..9277
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..427
                     /label=MT promoter
                     /note="Drosophila metallothionein promoter"
     polyA_signal    2582..2703
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3413..3430)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3584..4172)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3664..3683)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4343..5134,5135..5203))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4966..4985
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5204..5308)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5376..5394
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6790..7069
                     /label=copia promoter
                     /note="strong promoter from the Drosophila transposable 
                     element copia (Sinclair et al., 1986)"
     CDS             7115..8140
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7115..8140
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     intron          8503..8568
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8698..8718
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9143..9277
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 aattcgttgc aggacaggat gtggtgcccg atgtgactag ctctttgctg caggccgtcc
       61 tatcctctgg ttccgataag agacccagaa ctccggcccc ccaccgccca ccgccacccc
      121 catacatatg tggtacgcaa gtaagagtgc ctgcgcatgc cccatgtgcc ccaccaagag
      181 ctttgcatcc catacaagtc cccaaagtgg agaaccgaac caattcttcg cgggcagaac
      241 aaaagcttct gcacacgtct ccactcgaat ttggagccgg ccggcgtgtg caaaagaggt
      301 gaatcgaacg aaagacccgt gtgtaaagcc gcgtttccaa aatgtataaa accgagagca
      361 tctggccaat gtgcatcagt tgtggtcagc agcaaaatca agtgaatcat ctcagtgcaa
      421 ctaaaggggg gatctcgagc ctctgccatg actatcgtag agtaaaaagg gttaagcatt
      481 tccgaccata taaagtataa atactcttga tcagaatcaa tagccgagtc gaactggcca
      541 tgtccgcctc aacctatgag gattgaaaca agcattcatt ctgaatacat agacgcagcg
      601 caagtttgtt gactcatatt gtcacactca ctctaacgcc cacaaagcgc cttaacctgc
      661 cacggacacc tttttgaaat tcgaaatatt tcgttatctt ttcatatttt tattagtttt
      721 gtaaatttat atcgagttgc aaaaaaaact attccacgcc aactataact cccacaaacc
      781 gcccaaaaat gccacaccca ctcttttaag aaatgattca attttgttgg attaaataag
      841 tcgttttcct tatttcaatc tatatgccga aaacatattg accacgccca ctctgacccc
      901 taaaaccgcc caattttaaa aattcaaaaa acccttttac gcttaacaac tttttttgtt
      961 ttacaggcag gtaccatgga agatgccaaa aacattaaga agggcccagc gccattctac
     1021 ccactcgaag acgggaccgc cggcgagcag ctgcacaaag ccatgaagcg ctacgccctg
     1081 gtgcccggca ccatcgcctt taccgacgca catatcgagg tggacattac ctacgccgag
     1141 tacttcgaga tgagcgttcg gctggcagaa gctatgaagc gctatgggct gaatacaaac
     1201 catcggatcg tggtgtgcag cgagaatagc ttgcagttct tcatgcccgt gttgggtgcc
     1261 ctgttcatcg gtgtggctgt ggccccagct aacgacatct acaacgagcg cgagctgctg
     1321 aacagcatgg gcatcagcca gcccaccgtc gtattcgtga gcaagaaagg gctgcaaaag
     1381 atcctcaacg tgcaaaagaa gctaccgatc atacaaaaga tcatcatcat ggatagcaag
     1441 accgactacc agggcttcca aagcatgtac accttcgtga cttcccattt gccacccggc
     1501 ttcaacgagt acgacttcgt gcccgagagc ttcgaccggg acaaaaccat cgccctgatc
     1561 atgaacagta gtggcagtac cggattgccc aagggcgtag ccctaccgca ccgcaccgct
     1621 tgtgtccgat tcagtcatgc ccgcgacccc atcttcggca accagatcat ccccgacacc
     1681 gctatcctca gcgtggtgcc atttcaccac ggcttcggca tgttcaccac gctgggctac
     1741 ttgatctgcg gctttcgggt cgtgctcatg taccgcttcg aggaggagct attcttgcgc
     1801 agcttgcaag actataagat tcaatctgcc ctgctggtgc ccacactatt tcccggggag
     1861 ctccaggtat tgttttgaag atttttctgc ttatctatat actttaaagg gaacctggta
     1921 atcgaatatt cgatgcagtc caaacaagag tagtaaaaaa tcaacagaga acgctattcg
     1981 agtttttaga ctagactata tgtacacccg atatttagct gttacgagcg caacaatgaa
     2041 ctttcaaaat tgtgtgactg ttttggacgt tttgcaggtg taagtagggg cgtggccaaa
     2101 gtgtttttgg tatgtcaata aaaattggca agacaatgaa aacgaagatt aatcaaaaca
     2161 ttttttgtgg gggcgtggca aacagttttt tggcaaatcg attttatttt atcgatgaaa
     2221 taatatgaat acattttgca agagcgtggg agtggcagtt ctggacgctt tgtgggcgtt
     2281 agaggatgcg tagcaacaaa cttgcgctgc gatctatgct taatctcaac tttctagctt
     2341 ttagttagat agtttctgaa atctcgacgt tcatacaggc ggatatggcc agttcgactc
     2401 gtcttttgat gctaatcaag aatatatgta gtttatatgg tcggaaacgc ttccttctac
     2461 ctgttacata cctttaaaca aatctaatat acgctttccc tctacgagta atgggtatga
     2521 aaacaaaaat caacacgcat caacgcattt tggaaagttg aaggcgtgat agtgcggccg
     2581 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     2641 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     2701 ttaactagtg gatccactag gggccgccga cgcgaggctg gatggccttc cccattatga
     2761 ttcttctcgc ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg
     2821 tagatgacga ccatcaggga cagcttcaag gatcgctcgc ggctcttacc agcctaactt
     2881 cgatcattgg accgctgatc gtcacggcga tttatgccgc ctcggcgagc acatggaacg
     2941 ggttggcatg gattgtaggc gccgccctat accttgtctg cctccccgcg ttgcgtcgcg
     3001 gtgcatggag ccgggccacc tcgacctgaa tggaagccgg cggcacctcg ctaacggatt
     3061 caccactcca agaattggag ccaatcaatt cttgcggaga actgtgaatg cgcaaaccaa
     3121 cccttggcag aacatatcca tcgcgtccgc catctccagc agccgcacgc ggcgcatctc
     3181 gggcagcgtt gggtcctggc cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg
     3241 gctaggctgg cggggttgcc ttactggtta gcagaatgaa tcaccgatac gcgagcgaac
     3301 gtgaagcgac tgctgctgca aaacgtctgc gacctgagca acaacatgaa tggtcttcgg
     3361 tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg ctcttccgct tcctcgctca
     3421 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     3481 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     3541 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     3601 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     3661 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     3721 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     3781 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     3841 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     3901 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     3961 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     4021 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     4081 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     4141 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     4201 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     4261 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     4321 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     4381 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     4441 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     4501 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     4561 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     4621 cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct
     4681 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     4741 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     4801 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     4861 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     4921 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac
     4981 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     5041 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     5101 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     5161 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     5221 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     5281 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     5341 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     5401 gtcttcaaga actcacattt gtacgaattt tttttttatc aaaagttcga gtttttcacc
     5461 aatttcctca tcaaccgagc aaggcaaacg gctttgaata atatggtgtt atatatacat
     5521 atatcaaatc gctgctgact gcgtgattga tggccccaag attacatatt atcgaatcag
     5581 gattcagaag gagatcaatg tcaaatgcgg acaggaacat gaaagacgcc tgttatgcgc
     5641 aattaaaaat ttgggtttaa ttgctgtgga aactgttgtt ggcggcatct taagttcctg
     5701 tttaacaaca tcaactactt atgtacgtag aagcgtttaa gccatttgca tacagatgag
     5761 aactggcttt tgtgctaatc agtcaagatg actccgatga tgatgactca ttacctgacc
     5821 agttttcgct gctttctttt caacaactac ttgtatatgt attgtatcca atagcaatac
     5881 attgaatttc catggtctag tcacgtatta tcatttaatt gacaccaagt cgtgttattg
     5941 ttgagctatc gagttcagct caaacatttc ttattcccat gaataagccg gcaaaaatat
     6001 gcaatctatg aaagttaata taagcaaacc ttactttgac tcaataccaa tgcactttgt
     6061 gtcgataggt tcacgcaatt gaggcgatta ttccgataac ccaagcgatt gactgttccc
     6121 gtttcgattc caattgaaat ttggaaatgt acaatagttt tgctatatgc tgtcaagtac
     6181 gctcttatct tctctgggtt ttcttcagag tttcgaaacg cttcttcttt tttttgtttt
     6241 tttttttttg gaatctcgta ttttggaagg ggctcccctc tggaatttgt tacactgtcg
     6301 ttatcattgc gaacaagcgg cccgaagcta tcagcgactt taacatttac aatgcacttt
     6361 tttacgacca attaaatgta cattttcctt tcttcgcccg ttgataagcg aacgcgatgt
     6421 ggcgcaggca atgtgttgct cttgcgacac aaacgcaatc aaaatggatt caatttcgct
     6481 ttttcccagt gaaacgaaga acgaaccgac catcatgata tgctcctctg catgttgcgt
     6541 attgaatcaa tgacaatttc aattaagccg cccgttcgtc atgcgttttc gtgcgcttcg
     6601 aaatgctgat aacgctgctg tcctccaact gctttgcatg tggacacaat tccatttatt
     6661 taattctttt atttggatcg gttaaattaa aaagcgcctt gttacgcatt taacgttgtt
     6721 tccggtgcgt ggtggtttca tgcttctggg aacggcaaat gggtttagga ttgggaaccc
     6781 ctcatcatct gttggaatat actattcaac ctacaaaagt aacgttaaac aacactactt
     6841 tatatttgat atgaatggcc acacctttta tgccataaaa catattgtaa gagaatacca
     6901 ctctttttat tccttctttc cttcttgtac gttttttgct gtaagtaggt cgtggtgctg
     6961 gtgttgcagt tgaaataact taaaatataa atcataaaac tcaaacataa acttgactat
     7021 ttatttattt attaagaaag gaaatataaa ttataaatta caacaggtta tggacctgca
     7081 gccaagcttg gcgctcgtcc gggggcaatg agatatgaaa aagcctgaac tcaccgcgac
     7141 gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc
     7201 ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg
     7261 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc
     7321 ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta
     7381 ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc
     7441 cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca
     7501 gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga
     7561 tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac
     7621 cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc
     7681 cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg
     7741 ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt
     7801 cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt
     7861 cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat
     7921 tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc
     7981 gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat
     8041 cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg
     8101 aaaccgacgc cccagcactc gtccgagggc aaaggaatag agtagatgcc gaccgaacaa
     8161 gagctgattt cgagaacgcc tcagccagca actcgcgcga gcctagcaag gcaaatgcga
     8221 gagaacggcc ttacgcttgg tggcacagtt ctcgtccaca gttcgctaag ctcgctcggc
     8281 tgggtcgcgg gagggccggt cgcagtgatt caggcccttc tggattgtgt tggtccccag
     8341 ggcacgattg tcatgcccac gcactcgggt gatctgactg atcccgcaga ttggagatcg
     8401 ccgcccgtgc ctgccgattg ggtgcagatc tttgtgaagg aaccttactt ctgtggtgtg
     8461 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
     8521 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
     8581 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
     8641 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
     8701 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
     8761 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
     8821 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
     8881 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
     8941 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
     9001 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
     9061 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     9121 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     9181 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     9241 gtccaaactc atcaatgtat cttatcatgt ctggatc
//
