LOCUS       sequence-211087-        8434 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression plasmid for expressing circular RNAs of a desired 
            sequence in Drosophila using the dati flanking introns to drive 
            backsplicing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8434)
  AUTHORS   Huang C, Liang D, Tatomer DC, Wilusz JE
  TITLE     A length-dependent evolutionarily conserved pathway controls nuclear
            export of circular RNAs.
  JOURNAL   Genes Dev. 2018 May 17. pii: gad.314856.118. doi: 
            10.1101/gad.314856.118.
  PUBMED    29773557
REFERENCE   2  (bases 1 to 8434)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2018 May 17. pii: gad.314856.118. doi: 10.1101/gad.314856.118."
COMMENT     Sequence Label: Hy_pMT dati MCS Exon
FEATURES             Location/Qualifiers
     source          1..8434
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1026..1305
                     /label=copia promoter
                     /note="strong promoter from the Drosophila transposable 
                     element copia (Sinclair et al., 1986)"
     CDS             1351..2376
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             1351..2376
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     intron          2739..2804
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2934..2954
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3379..3513
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        3514..3940
                     /label=MT promoter
                     /note="Drosophila metallothionein promoter"
     polyA_signal    5252..5373
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6083..6100)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6254..6842)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6334..6353)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7013..7804,7805..7873))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7636..7655
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7874..7978)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8046..8064
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggcttttgtg ctaatcagtc aagatgactc cgatgatgat gactcattac ctgaccagtt
       61 ttcgctgctt tcttttcaac aactacttgt atatgtattg tatccaatag caatacattg
      121 aatttccatg gtctagtcac gtattatcat ttaattgaca ccaagtcgtg ttattgttga
      181 gctatcgagt tcagctcaaa catttcttat tcccatgaat aagccggcaa aaatatgcaa
      241 tctatgaaag ttaatataag caaaccttac tttgactcaa taccaatgca ctttgtgtcg
      301 ataggttcac gcaattgagg cgattattcc gataacccaa gcgattgact gttcccgttt
      361 cgattccaat tgaaatttgg aaatgtacaa tagttttgct atatgctgtc aagtacgctc
      421 ttatcttctc tgggttttct tcagagtttc gaaacgcttc ttcttttttt tgtttttttt
      481 tttttggaat ctcgtatttt ggaaggggct cccctctgga atttgttaca ctgtcgttat
      541 cattgcgaac aagcggcccg aagctatcag cgactttaac atttacaatg cactttttta
      601 cgaccaatta aatgtacatt ttcctttctt cgcccgttga taagcgaacg cgatgtggcg
      661 caggcaatgt gttgctcttg cgacacaaac gcaatcaaaa tggattcaat ttcgcttttt
      721 cccagtgaaa cgaagaacga accgaccatc atgatatgct cctctgcatg ttgcgtattg
      781 aatcaatgac aatttcaatt aagccgcccg ttcgtcatgc gttttcgtgc gcttcgaaat
      841 gctgataacg ctgctgtcct ccaactgctt tgcatgtgga cacaattcca tttatttaat
      901 tcttttattt ggatcggtta aattaaaaag cgccttgtta cgcatttaac gttgtttccg
      961 gtgcgtggtg gtttcatgct tctgggaacg gcaaatgggt ttaggattgg gaacccctca
     1021 tcatctgttg gaatatacta ttcaacctac aaaagtaacg ttaaacaaca ctactttata
     1081 tttgatatga atggccacac cttttatgcc ataaaacata ttgtaagaga ataccactct
     1141 ttttattcct tctttccttc ttgtacgttt tttgctgtaa gtaggtcgtg gtgctggtgt
     1201 tgcagttgaa ataacttaaa atataaatca taaaactcaa acataaactt gactatttat
     1261 ttatttatta agaaaggaaa tataaattat aaattacaac aggttatgga cctgcagcca
     1321 agcttggcgc tcgtccgggg gcaatgagat atgaaaaagc ctgaactcac cgcgacgtct
     1381 gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag
     1441 ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta
     1501 aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc
     1561 gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc
     1621 atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct
     1681 gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg
     1741 agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc
     1801 atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc
     1861 agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa
     1921 gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc
     1981 ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc
     2041 aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag
     2101 cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt
     2161 cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag
     2221 ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc
     2281 cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac
     2341 cgacgcccca gcactcgtcc gagggcaaag gaatagagta gatgccgacc gaacaagagc
     2401 tgatttcgag aacgcctcag ccagcaactc gcgcgagcct agcaaggcaa atgcgagaga
     2461 acggccttac gcttggtggc acagttctcg tccacagttc gctaagctcg ctcggctggg
     2521 tcgcgggagg gccggtcgca gtgattcagg cccttctgga ttgtgttggt ccccagggca
     2581 cgattgtcat gcccacgcac tcgggtgatc tgactgatcc cgcagattgg agatcgccgc
     2641 ccgtgcctgc cgattgggtg cagatctttg tgaaggaacc ttacttctgt ggtgtgacat
     2701 aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg
     2761 tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca acctatggaa
     2821 ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag
     2881 aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa
     2941 agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc
     3001 atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag
     3061 ctgcactgct atacaagaaa attatggaaa aatattctgt aacctttata agtaggcata
     3121 acagttataa tcataacata ctgttttttc ttactccaca caggcataga gtgtctgcta
     3181 ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata
     3241 aggaatattt gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta
     3301 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     3361 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     3421 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     3481 aaactcatca atgtatctta tcatgtctgg atcaattcgt tgcaggacag gatgtggtgc
     3541 ccgatgtgac tagctctttg ctgcaggccg tcctatcctc tggttccgat aagagaccca
     3601 gaactccggc cccccaccgc ccaccgccac ccccatacat atgtggtacg caagtaagag
     3661 tgcctgcgca tgccccatgt gccccaccaa gagctttgca tcccatacaa gtccccaaag
     3721 tggagaaccg aaccaattct tcgcgggcag aacaaaagct tctgcacacg tctccactcg
     3781 aatttggagc cggccggcgt gtgcaaaaga ggtgaatcga acgaaagacc cgtgtgtaaa
     3841 gccgcgtttc caaaatgtat aaaaccgaga gcatctggcc aatgtgcatc agttgtggtc
     3901 agcagcaaaa tcaagtgaat catctcagtg caactaaagg ggggatctcg agcctctgcc
     3961 atgactatcg tagagtaaaa agggttaagc atttccgacc atataaagta taaatactct
     4021 tgatcagaat caatagccga gtcgaactgg ccatgtccgc ctcaacctat gaggattgaa
     4081 acaagcattc attctgaata catagacgca gcgcaagttt gttgactcat attgtcacac
     4141 tcactctaac gcccacaaag cgccttaacc tgccacggac acctttttga aattcgaaat
     4201 atttcgttat cttttcatat ttttattagt tttgtaaatt tatatcgagt tgcaaaaaaa
     4261 actattccac gccaactata actcccacaa accgcccaaa aatgccacac ccactctttt
     4321 aagaaatgat tcaattttgt tggattaaat aagtcgtttt ccttatttca atctatatgc
     4381 cgaaaacata ttgaccacgc ccactctgac ccctaaaacc gcccaatttt aaaaattcaa
     4441 aaaacccttt tacgcttaac aacttttttt gttttacagg caggtaccac cggtttaatt
     4501 aaggtaacca tgcatgctag ccccggggag ctccaggtat tgttttgaag atttttctgc
     4561 ttatctatat actttaaagg gaacctggta atcgaatatt cgatgcagtc caaacaagag
     4621 tagtaaaaaa tcaacagaga acgctattcg agtttttaga ctagactata tgtacacccg
     4681 atatttagct gttacgagcg caacaatgaa ctttcaaaat tgtgtgactg ttttggacgt
     4741 tttgcaggtg taagtagggg cgtggccaaa gtgtttttgg tatgtcaata aaaattggca
     4801 agacaatgaa aacgaagatt aatcaaaaca ttttttgtgg gggcgtggca aacagttttt
     4861 tggcaaatcg attttatttt atcgatgaaa taatatgaat acattttgca agagcgtggg
     4921 agtggcagtt ctggacgctt tgtgggcgtt agaggatgcg tagcaacaaa cttgcgctgc
     4981 gatctatgct taatctcaac tttctagctt ttagttagat agtttctgaa atctcgacgt
     5041 tcatacaggc ggatatggcc agttcgactc gtcttttgat gctaatcaag aatatatgta
     5101 gtttatatgg tcggaaacgc ttccttctac ctgttacata cctttaaaca aatctaatat
     5161 acgctttccc tctacgagta atgggtatga aaacaaaaat caacacgcat caacgcattt
     5221 tggaaagttg aaggcgtgat agtgcggccg caacttgttt attgcagctt ataatggtta
     5281 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     5341 ttgtggtttg tccaaactca tcaatgtatc ttaactagtg gatccactag gggccgccga
     5401 cgcgaggctg gatggccttc cccattatga ttcttctcgc ttccggcggc atcgggatgc
     5461 ccgcgttgca ggccatgctg tccaggcagg tagatgacga ccatcaggga cagcttcaag
     5521 gatcgctcgc ggctcttacc agcctaactt cgatcattgg accgctgatc gtcacggcga
     5581 tttatgccgc ctcggcgagc acatggaacg ggttggcatg gattgtaggc gccgccctat
     5641 accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag ccgggccacc tcgacctgaa
     5701 tggaagccgg cggcacctcg ctaacggatt caccactcca agaattggag ccaatcaatt
     5761 cttgcggaga actgtgaatg cgcaaaccaa cccttggcag aacatatcca tcgcgtccgc
     5821 catctccagc agccgcacgc ggcgcatctc gggcagcgtt gggtcctggc cacgggtgcg
     5881 catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc ttactggtta
     5941 gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc
     6001 gacctgagca acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg
     6061 gaagtcagcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     6121 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     6181 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     6241 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     6301 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     6361 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     6421 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     6481 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     6541 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     6601 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     6661 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     6721 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     6781 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     6841 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     6901 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     6961 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     7021 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     7081 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     7141 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     7201 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     7261 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     7321 ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     7381 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     7441 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     7501 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     7561 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     7621 cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     7681 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     7741 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     7801 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     7861 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     7921 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     7981 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     8041 ataaaaatag gcgtatcacg aggccctttc gtcttcaaga actcacattt gtacgaattt
     8101 tttttttatc aaaagttcga gtttttcacc aatttcctca tcaaccgagc aaggcaaacg
     8161 gctttgaata atatggtgtt atatatacat atatcaaatc gctgctgact gcgtgattga
     8221 tggccccaag attacatatt atcgaatcag gattcagaag gagatcaatg tcaaatgcgg
     8281 acaggaacat gaaagacgcc tgttatgcgc aattaaaaat ttgggtttaa ttgctgtgga
     8341 aactgttgtt ggcggcatct taagttcctg tttaacaaca tcaactactt atgtacgtag
     8401 aagcgtttaa gccatttgca tacagatgag aact
//
