LOCUS       sequence-211089-         531 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  empty sgRNA cloning vector with MS2-AIDmono.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Liu LD, Huang M, Dai P, Liu T, Fan S, Cheng X, Zhao Y, Yeap LS, Meng
            FL
  TITLE     Intrinsic Nucleotide Preference of Diversifying Base Editors Guides 
            Antibody Ex Vivo Affinity Maturation.
  JOURNAL   Cell Rep. 2018 Oct 23;25(4):884-892.e3. doi: 
            10.1016/j.celrep.2018.09.090.
  PUBMED    30355495
REFERENCE   2  (bases 1 to 531)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.09"; journalName: "Cell Rep"; date: 
            "2018-10-23- 23"; volume: "25"; issue: "4"; pages: "884-892.e3"
COMMENT     Sequence Label: pLL1 sgRNA(MS2)-MS2-AIDmono
FEATURES             Location/Qualifiers
     source          1..531
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggaccccg ctaccttcac ataccaattc aaaaatgtcc gctgggctaa gggtcggcgt
       61 gagacctacc tgtgctacgt agtgaagagg cgtgacagtg ctacatcctt ttcactggac
      121 tttggttatc ttcgcaataa gaacggctgc cacgtggaat tgctcttcct ccgctacatc
      181 tcggactggg acctagaccc tggccgctgc taccgcgtca cctggttcac ctcctggagc
      241 ccctgctacg actgtgcccg acatgtggcc gactttctgc gagggaaccc caacctcagt
      301 ctgaggatct tcaccgcgcg cctctacttc tgtgaggacc gcaaggctga gcccgagggg
      361 ctgcggcggc tggcagaagc cggggtgcaa atagccatca tgaccttcaa agattatttt
      421 tactgctgga atacttttgt agaaaaccat gaaagaactt tcaaagcctg ggaagggctg
      481 catgaaaatt cagttcgtct ctccagacag cttcggcgca tccttcagta a
//
