LOCUS       sequence-211097-         600 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  empty sgRNA cloning vector with MS2-humanA3a D131A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Liu LD, Huang M, Dai P, Liu T, Fan S, Cheng X, Zhao Y, Yeap LS, Meng
            FL
  TITLE     Intrinsic Nucleotide Preference of Diversifying Base Editors Guides 
            Antibody Ex Vivo Affinity Maturation.
  JOURNAL   Cell Rep. 2018 Oct 23;25(4):884-892.e3. doi: 
            10.1016/j.celrep.2018.09.090.
  PUBMED    30355495
REFERENCE   2  (bases 1 to 600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.09"; journalName: "Cell Rep"; date: 
            "2018-10-23- 23"; volume: "25"; issue: "4"; pages: "884-892.e3"
COMMENT     Sequence Label: pLL5 sgRNA(MS2)-MS2-hA3a D131A
FEATURES             Location/Qualifiers
     source          1..600
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..600
                     /codon_start=1
                     /gene="human APOBEC3A"
                     /gene_synonym="A3A"
                     /product="human cytidine deaminase (C to U editing enzyme) 
                     that efficiently edits methylated cytosines (Wang et al., 
                     2018)"
                     /label=APOBEC3A
                     /note="also known as hA3A"
                     /translation="MEASPASGPRHLMDPHIFTSNFNNGIGRHKTYLCYEVERLDNGTS
                     VKMDQHRGFLHNQAKNLLCGFYGRHAELRFLDLVPSLQLDPAQIYRVTWFISWSPCFSW
                     GCAGEVRAFLQENTHVRLRIFAARIYAYDPLYKEALQMLRDAGAQVSIMTYDEFKHCWD
                     TFVDHQGCPFQPWDGLDEHSQALSGRLRAILQNQGN"
ORIGIN
        1 atggaagcca gcccagcatc cgggcccaga cacttgatgg atccacacat attcacttcc
       61 aactttaaca atggcattgg aaggcataag acctacctgt gctacgaagt ggagcgcctg
      121 gacaatggca cctcggtcaa gatggaccag cacaggggct ttctacacaa ccaggctaag
      181 aatcttctct gtggctttta cggccgccat gcggagctgc gcttcttgga cctggttcct
      241 tctttgcagt tggacccggc ccagatctac agggtcactt ggttcatctc ctggagcccc
      301 tgcttctcct ggggctgtgc cggggaagtg cgtgcgttcc ttcaggagaa cacacacgtg
      361 agactgcgta tcttcgctgc ccgcatctat gcttacgacc ccctatataa ggaggcactg
      421 caaatgctgc gggatgctgg ggcccaagtc tccatcatga cctacgatga atttaagcac
      481 tgctgggaca cctttgtgga ccaccaggga tgtcccttcc agccctggga tggactagat
      541 gagcacagcc aagccctgag tgggaggctg cgggccattc tccagaatca gggaaactga
//
