LOCUS       sequence-211116-       11775 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral Evi-1-Flag with dual BFP and puro markers.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11775)
  TITLE     Perkins lab unpublished plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pKAM-E-Flag-BFP
FEATURES             Location/Qualifiers
     source          1..11775
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    185..300
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        378..589
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          590..1528
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1485..1505
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4695..4718
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     5137..5156
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5244..5840
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5244..5263)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5740..5760
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             5859..5912
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5913..6614
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     LTR             6696..7329
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7457..7473)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7457..7473)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7470..7492)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7481..7497
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7505..7511,7512..7529,7530..7535))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7550..7571
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7688..7705)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7859..8447)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7939..7958)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8618..9409,9410..9478))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9241..9260
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9479..9583)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9631..9765
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9668..9687)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9722..9741
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9933..10566
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10613..10738
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11235..11468
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11653..11697
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
       61 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
      121 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat
      181 cgcctttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
      241 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
      301 tcgggtttat tacagggaca gcagagatcc agtttatcga tgaggccctt tcgtcttcac
      361 tcgaggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
      421 aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
      481 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
      541 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt
      601 gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac
      661 ttccacctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa gtgggtggga
      721 gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc
      781 tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt
      841 cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca
      901 agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt ttggggccgc
      961 gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc
     1021 gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg
     1081 cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt
     1141 tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa aatggaggac
     1201 gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg cctttccgtc
     1261 ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta
     1321 gttctcgagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga
     1381 gtttccccac actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt
     1441 ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt
     1501 ggttcaaagt ttttttcttc catttcaggt gtcgtgagga tcccagcatg agatccaaaa
     1561 agagtgaaga ggacccgcac gaacccatgg cgcctgacat ccacgaagaa cggcagcacc
     1621 gctgtgagga ctgtgaccag ctctttgaat ccaaggcaga gctagccgat caccagaagt
     1681 tcccatgcag cacacctcac tcggccttct ccatggtgga ggaggacttg caacaaaacc
     1741 tggagagtga gagcgatctc cgagagatcc atggcaacca ggactgtaag gaatgtgacc
     1801 gagttttccc cgatctgcaa agcttggaga agcacatgct gtcacatact gaggagaggg
     1861 aatacaagtg tgatcagtgt cccaaggcat ttaactggaa gtccaattta attcgccacc
     1921 agatgtcaca tgacagtgga aagcactatg agtgtgaaaa ctgtgccaag gttttcacgg
     1981 accctagcaa ccttcagcga cacattcgat ctcagcatgt tggtgcccgg gctcatgctt
     2041 gccccgagtg tggtaaaaca tttgccactt cgtcaggcct caaacagcac aagcacatcc
     2101 acagcagtgt gaagcccttt atctgtgagg tctgccataa atcctatact cagttttcaa
     2161 acctttgtcg tcataagcgc atgcatgctg attgcagaac ccaaatcaag tgcaaagact
     2221 gtggacaaat gttcagcact acgtcttcct taaataaaca caggaggttt tgtgagggca
     2281 agaaccattt tgcggcaggt ggattttttg gccaaggcat ttcacttcct ggaaccccag
     2341 ctatggataa aacgtccatg gttaatatga gtcatgccaa cccgggcctg gctgactatt
     2401 ttggcaccaa taggcatcct gctggtctta cctttccaac agctcctgga ttttccttta
     2461 gctttcctgg tctgtttcct tcgggcttat accacaggcc tcctttgata cccgctagtc
     2521 ctcctgttaa aggattatca agtaccgaac agtcaaacaa atgtcaaagt cccctcttga
     2581 cacatcctca gatactgcca gctacacagg atattttgaa ggcactatct aaacacccac
     2641 ctgtagggga caataagcca gtggaactgc tgcccgagag gtcctctgaa gagaggcccc
     2701 ttgagaaaat cagtgaccag tcagagagta gtgaccttga tgatgtcagt acaccaagtg
     2761 gcagtgacct ggaaacaacc tcgggctctg atctggaaag tgaccttgaa agtgataaag
     2821 agaaatgtaa agaaaatggt aaaatgttca aagacaaagt aagccctctg cagaatctgg
     2881 cttcgataac taataagaaa gaacacaaca atcattccgt tttctcagca tctgtagagg
     2941 agcaaagtgc cgtgtcagga gctgtgaatg attctataaa ggctattgcg tctattgctg
     3001 aaaaatactt tggttctaca ggactggtgg ggctgcaaga caaaaaagtt ggagcgttac
     3061 cttacccttc catgtttccc ctcccgtttt ttccagcatt ctctcaatca atgtacccat
     3121 ttcctgatag agacttgagg tcgttacctt tgaaaatgga gccccaatca ccaagtgaag
     3181 ttaagaaact gcagaaggga agctctgagt ccccttttga cctcaccact aagagaaagg
     3241 atgagaagcc cttgacttca ggcccctcga agccttcagg aacaccagcc acaagccaag
     3301 accagcccct ggatctaagt atgggcagta ggggtagagc cagtgggaca aagttgactg
     3361 agcctcgaaa aaaccatgtg tttggggaaa agaaaggaag caacatggat actaggccat
     3421 cttcagatgg ctccttgcag catgccagac ccactccctt cttcatggac cccatttata
     3481 gagtagagaa aagaaagtta actgacccgc ttgaagcttt gaaagaaaaa tacttgagac
     3541 cttctccagg attcttgttt cacccgcaaa tgtcagcaat tgagaacatg gcagaaaagc
     3601 tggaaagctt cagcgccctc aaacctgagg ccagcgagct cctgcagtcc gtgccctcca
     3661 tgttcagctt ccgagctcct cccaacaccc tgccagagaa cctgctgcgg aaggggaaag
     3721 agcgctacac ctgcaggtac tgtggcaaga tatttccaag gtctgcgaac ctaacacggc
     3781 acttgagaac ccacacagga gagcaacctt acagatgcaa atactgtgat agatcattca
     3841 gcatttcttc caacctgcag cgacatgtgc gcaacatcca caacaaggag aagccattta
     3901 agtgtcattt atgtgacaga tgttttggtc aacaaaccaa tcttgacaga cacctgaaga
     3961 aacatgagaa cggcaacatg tctgggacgg caacgtcctc gcctcactca gagctagaaa
     4021 gcgcaggcgc aatcctggat gacaaagaag atgcgtactt tacagagatc cgcaatttca
     4081 tcgggaacag caaccatggt agccagtctc ctcggaacat ggaagagagg atgaatggca
     4141 gtcacttcaa ggataaaaag gctttggcaa ccagccaaaa ttcagattta ttggatgatg
     4201 aagaagtaga agatgaggtg ttgttggatg aggaggatga agacaatgat attcctggaa
     4261 agcccagaaa ggagctaggg gtgactcgtt tagacgagga gatcccggag gatgactacg
     4321 aagaagctgg tgccctggag atgagctgta aggcgtcccc ggtgaggtat aaagaggaag
     4381 actataaatc tggcctttct gctctagatc acataaggca cttcacagat agcctcaaaa
     4441 tgagggaaat ggaagagaat caatacactg acgctgagct gtcctccatt agttcttctc
     4501 atgtgccaga ggagcttaaa cagacgttac acagaaagtc caaatcacag gcatatgcta
     4561 tgatgttgtc actgtctgac aaggattccc tccatcccac ctcccacagt tcctccaacg
     4621 tgtggcacag catggcaagg gctgcagcag aatccagtgc catccagtcc ataagccatg
     4681 taggcagcgg cgtagattac aaggatgacg acgataagta ggcgaattct accgggtagg
     4741 ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg
     4801 gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc
     4861 ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt
     4921 cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac
     4981 tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag
     5041 cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc
     5101 cgggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca
     5161 cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc atccatctag
     5221 atctcgagca gctgaagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc
     5281 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
     5341 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct
     5401 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
     5461 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
     5521 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
     5581 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
     5641 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
     5701 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
     5761 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
     5821 tgacccgcaa gcccggtgcc ggcggcgggt ccggaggaga gggcagagga agtctcctaa
     5881 catgcggtga cgtggaggag aatcctggcc caatgagcga gctgattaag gagaacatgc
     5941 acatgaagct gtacatggag ggcaccgtgg acaaccatca cttcaagtgc acatccgagg
     6001 gcgaaggcaa gccctacgag ggcacccaga ccatgagaat caaggtggtc gagggcggcc
     6061 ctctcccctt cgccttcgac atcctggcta ctagcttcct ctacggcagc aagaccttca
     6121 tcaaccacac ccagggcatc cccgacttct tcaagcagtc cttccctgag ggcttcacat
     6181 gggagagagt caccacatac gaggacgggg gcgtgctgac cgctacccag gacaccagcc
     6241 tccaggacgg ctgcctcatc tacaacgtca agatcagagg ggtgaacttc acatccaacg
     6301 gccctgtgat gcagaagaaa acactcggct gggaggcctt caccgagacg ctgtaccccg
     6361 ctgacggcgg cctggaaggc agaaacgaca tggccctgaa gctcgtgggc gggagccatc
     6421 tgatcgcaaa catcaagacc acatatagat ccaagaaacc cgctaagaac ctcaagatgc
     6481 ctggcgtcta ctatgtggac tacagactgg aaagaatcaa ggaggccaac aacgagacct
     6541 acgtcgagca gcacgaggtg gcagtggcca gatactgcga cctccctagc aaactggggc
     6601 acaagcttaa ttgagcggcc gctaggtacc tttaagacca atgacttaca aggcagctgt
     6661 agatcttagc cactttttaa aagaaaagag gggactggaa gggctaattc actcccaacg
     6721 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca
     6781 gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct
     6841 agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt
     6901 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt
     6961 tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa
     7021 ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg
     7081 cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg
     7141 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     7201 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     7261 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     7321 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     7381 gaatatcaga gagtgagagg ccttgacatt gctagcgttt accgtcgacc tctagctaga
     7441 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     7501 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     7561 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     7621 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     7681 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     7741 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     7801 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     7861 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     7921 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     7981 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     8041 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     8101 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     8161 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     8221 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     8281 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     8341 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     8401 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     8461 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     8521 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     8581 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     8641 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     8701 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     8761 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     8821 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     8881 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     8941 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     9001 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     9061 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     9121 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     9181 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     9241 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     9301 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     9361 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     9421 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     9481 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     9541 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     9601 tgccacctga cgtcgacgga tcgggagatc aacttgttta ttgcagctta taatggttac
     9661 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     9721 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa
     9781 gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg
     9841 tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt
     9901 atttgttaaa tatgtactac aaacttagta gttggaaggg ctaattcact cccaaagaag
     9961 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa
    10021 ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt
    10081 accagttgag ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca
    10141 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga
    10201 cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg
    10261 ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct
    10321 gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct
    10381 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
    10441 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
    10501 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
    10561 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
    10621 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
    10681 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
    10741 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
    10801 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
    10861 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
    10921 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
    10981 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
    11041 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat cttcagacct
    11101 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
    11161 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
    11221 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
    11281 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
    11341 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
    11401 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
    11461 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
    11521 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
    11581 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
    11641 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
    11701 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
    11761 ggcttggtag gttta
//
