LOCUS       sequence-211119-       11172 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral Evi-1-Flag with GFP marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11172)
  TITLE     Perkins lab unpublished plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11172)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pKAM-E-Flag-GFP
FEATURES             Location/Qualifiers
     source          1..11172
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          176..1114
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1071..1091
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4281..4304
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     4738..4756
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      4835..4844
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4841..4843,4844..4846,4847..5560)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4841..4843,4844..4846,4847..5557)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4886..4907)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5147..5166)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5494..5515
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5679..6312
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6440..6456)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6440..6456)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6453..6475)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6464..6480
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6488..6494,6495..6512,6513..6518))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6533..6554
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6671..6688)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6842..7430)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6922..6941)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7601..8392,8393..8461))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8224..8243
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8462..8566)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    8614..8748
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8651..8670)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8705..8724
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8916..9549
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9596..9721
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10218..10451
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10636..10680
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10943..11058
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        join(11136..11172,1..175)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga
       61 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
      121 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag
      181 tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg
      241 aattacttcc acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg
      301 gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc
      361 ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc
      421 tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt
      481 ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg
      541 ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct
      601 gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt
      661 gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc
      721 accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg
      781 gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt
      841 tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct
      901 cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc
      961 gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat
     1021 gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca
     1081 gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgaggatccc agcatgagat
     1141 ccaaaaagag tgaagaggac ccgcacgaac ccatggcgcc tgacatccac gaagaacggc
     1201 agcaccgctg tgaggactgt gaccagctct ttgaatccaa ggcagagcta gccgatcacc
     1261 agaagttccc atgcagcaca cctcactcgg ccttctccat ggtggaggag gacttgcaac
     1321 aaaacctgga gagtgagagc gatctccgag agatccatgg caaccaggac tgtaaggaat
     1381 gtgaccgagt tttccccgat ctgcaaagct tggagaagca catgctgtca catactgagg
     1441 agagggaata caagtgtgat cagtgtccca aggcatttaa ctggaagtcc aatttaattc
     1501 gccaccagat gtcacatgac agtggaaagc actatgagtg tgaaaactgt gccaaggttt
     1561 tcacggaccc tagcaacctt cagcgacaca ttcgatctca gcatgttggt gcccgggctc
     1621 atgcttgccc cgagtgtggt aaaacatttg ccacttcgtc aggcctcaaa cagcacaagc
     1681 acatccacag cagtgtgaag ccctttatct gtgaggtctg ccataaatcc tatactcagt
     1741 tttcaaacct ttgtcgtcat aagcgcatgc atgctgattg cagaacccaa atcaagtgca
     1801 aagactgtgg acaaatgttc agcactacgt cttccttaaa taaacacagg aggttttgtg
     1861 agggcaagaa ccattttgcg gcaggtggat tttttggcca aggcatttca cttcctggaa
     1921 ccccagctat ggataaaacg tccatggtta atatgagtca tgccaacccg ggcctggctg
     1981 actattttgg caccaatagg catcctgctg gtcttacctt tccaacagct cctggatttt
     2041 cctttagctt tcctggtctg tttccttcgg gcttatacca caggcctcct ttgatacccg
     2101 ctagtcctcc tgttaaagga ttatcaagta ccgaacagtc aaacaaatgt caaagtcccc
     2161 tcttgacaca tcctcagata ctgccagcta cacaggatat tttgaaggca ctatctaaac
     2221 acccacctgt aggggacaat aagccagtgg aactgctgcc cgagaggtcc tctgaagaga
     2281 ggccccttga gaaaatcagt gaccagtcag agagtagtga ccttgatgat gtcagtacac
     2341 caagtggcag tgacctggaa acaacctcgg gctctgatct ggaaagtgac cttgaaagtg
     2401 ataaagagaa atgtaaagaa aatggtaaaa tgttcaaaga caaagtaagc cctctgcaga
     2461 atctggcttc gataactaat aagaaagaac acaacaatca ttccgttttc tcagcatctg
     2521 tagaggagca aagtgccgtg tcaggagctg tgaatgattc tataaaggct attgcgtcta
     2581 ttgctgaaaa atactttggt tctacaggac tggtggggct gcaagacaaa aaagttggag
     2641 cgttacctta cccttccatg tttcccctcc cgttttttcc agcattctct caatcaatgt
     2701 acccatttcc tgatagagac ttgaggtcgt tacctttgaa aatggagccc caatcaccaa
     2761 gtgaagttaa gaaactgcag aagggaagct ctgagtcccc ttttgacctc accactaaga
     2821 gaaaggatga gaagcccttg acttcaggcc cctcgaagcc ttcaggaaca ccagccacaa
     2881 gccaagacca gcccctggat ctaagtatgg gcagtagggg tagagccagt gggacaaagt
     2941 tgactgagcc tcgaaaaaac catgtgtttg gggaaaagaa aggaagcaac atggatacta
     3001 ggccatcttc agatggctcc ttgcagcatg ccagacccac tcccttcttc atggacccca
     3061 tttatagagt agagaaaaga aagttaactg acccgcttga agctttgaaa gaaaaatact
     3121 tgagaccttc tccaggattc ttgtttcacc cgcaaatgtc agcaattgag aacatggcag
     3181 aaaagctgga aagcttcagc gccctcaaac ctgaggccag cgagctcctg cagtccgtgc
     3241 cctccatgtt cagcttccga gctcctccca acaccctgcc agagaacctg ctgcggaagg
     3301 ggaaagagcg ctacacctgc aggtactgtg gcaagatatt tccaaggtct gcgaacctaa
     3361 cacggcactt gagaacccac acaggagagc aaccttacag atgcaaatac tgtgatagat
     3421 cattcagcat ttcttccaac ctgcagcgac atgtgcgcaa catccacaac aaggagaagc
     3481 catttaagtg tcatttatgt gacagatgtt ttggtcaaca aaccaatctt gacagacacc
     3541 tgaagaaaca tgagaacggc aacatgtctg ggacggcaac gtcctcgcct cactcagagc
     3601 tagaaagcgc aggcgcaatc ctggatgaca aagaagatgc gtactttaca gagatccgca
     3661 atttcatcgg gaacagcaac catggtagcc agtctcctcg gaacatggaa gagaggatga
     3721 atggcagtca cttcaaggat aaaaaggctt tggcaaccag ccaaaattca gatttattgg
     3781 atgatgaaga agtagaagat gaggtgttgt tggatgagga ggatgaagac aatgatattc
     3841 ctggaaagcc cagaaaggag ctaggggtga ctcgtttaga cgaggagatc ccggaggatg
     3901 actacgaaga agctggtgcc ctggagatga gctgtaaggc gtccccggtg aggtataaag
     3961 aggaagacta taaatctggc ctttctgctc tagatcacat aaggcacttc acagatagcc
     4021 tcaaaatgag ggaaatggaa gagaatcaat acactgacgc tgagctgtcc tccattagtt
     4081 cttctcatgt gccagaggag cttaaacaga cgttacacag aaagtccaaa tcacaggcat
     4141 atgctatgat gttgtcactg tctgacaagg attccctcca tcccacctcc cacagttcct
     4201 ccaacgtgtg gcacagcatg gcaagggctg cagcagaatc cagtgccatc cagtccataa
     4261 gccatgtagg cagcggcgta gattacaagg atgacgacga taagtaggcg aattctaccg
     4321 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg
     4381 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg
     4441 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag
     4501 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg
     4561 tctcactagt accctcgcag acggacagcg ccagggagca atggcagcgc gccgaccgcg
     4621 atgggctgtg gccaatagcg gctgctcagc agggcgcgcc gagagcagcg gccgggaagg
     4681 ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg
     4741 ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat
     4801 caccgacctc tctccccagg gggatccacc ggtcgccacc atggtgagca agggcgagga
     4861 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     4921 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     4981 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     5041 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     5101 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     5161 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     5221 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     5281 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     5341 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     5401 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     5461 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     5521 cgccgggatc actctcggca tggacgagct gtacaagtaa agcggccgcg tcgacgggcc
     5581 ctcgacgggc ccgcggaatt cgagctcggt acctttaaga ccaatgactt acaaggcagc
     5641 tgtagatctt agccactttt taaaagaaaa gaggggactg gaagggctaa ttcactccca
     5701 acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta
     5761 gcagaactac acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa
     5821 gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt
     5881 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag
     5941 gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa
     6001 gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg
     6061 tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt
     6121 ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     6181 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     6241 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     6301 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     6361 aatgaatatc agagagtgag aggccttgac attgctagcg tttaccgtcg acctctagct
     6421 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     6481 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     6541 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     6601 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     6661 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     6721 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     6781 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     6841 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     6901 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     6961 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     7021 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     7081 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     7141 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     7201 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     7261 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     7321 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     7381 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     7441 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     7501 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     7561 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     7621 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     7681 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     7741 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     7801 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     7861 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     7921 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     7981 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     8041 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     8101 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     8161 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     8221 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     8281 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     8341 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     8401 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     8461 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     8521 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     8581 aagtgccacc tgacgtcgac ggatcgggag atcaacttgt ttattgcagc ttataatggt
     8641 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     8701 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact
     8761 caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac
     8821 ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat
     8881 tgtatttgtt aaatatgtac tacaaactta gtagttggaa gggctaattc actcccaaag
     8941 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca
     9001 gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct
     9061 agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt
     9121 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt
     9181 tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa
     9241 ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg
     9301 cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg
     9361 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     9421 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     9481 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     9541 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     9601 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     9661 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     9721 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     9781 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     9841 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     9901 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     9961 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
    10021 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccggccgct gatcttcaga
    10081 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
    10141 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
    10201 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
    10261 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
    10321 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
    10381 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
    10441 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
    10501 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
    10561 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
    10621 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
    10681 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
    10741 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
    10801 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
    10861 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
    10921 aacggatctc gacggtatcg cctttaaaag aaaagggggg attggggggt acagtgcagg
    10981 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
    11041 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttatcgatg
    11101 aggccctttc gtcttcactc gaggtgcccg tcagtgggca gagcgcacat cgcccacagt
    11161 ccccgagaag tt
//
