LOCUS       sequence-211126-        7350 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of His tagged mouse CBP HAT Brd-ZZ(1079-1751) 
            1557-1617 replaced by SGGSG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7350)
  AUTHORS   Park S, Stanfield RL, Martinez-Yamout MA, Dyson HJ, Wilson IA, 
            Wright PE
  TITLE     Role of the CBP catalytic core in intramolecular SUMOylation and 
            control of histone H3 acetylation.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Jun 19. pii: 201703105. doi: 
            10.1073/pnas.1703105114.
  PUBMED    28630323
REFERENCE   2  (bases 1 to 7350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2017 Jun 19. pii: 201703105. doi: 
            10.1073/pnas.1703105114."
COMMENT     Sequence Label: His6tev CBP BCHZDeltaAL
FEATURES             Location/Qualifiers
     source          1..7350
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    20..44
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             59..81
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             101..118
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             140..160
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     regulatory      1637..1646
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2082..2099
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2152..2170)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2166..2213
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      2250..2705
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2337..2356)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2547..2568
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2731..2835
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2836..2904,2905..3696)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3054..3073)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3867..4455
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4356..4375
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4609..4626
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4641..4780
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4706..4725)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4866..4888
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(4882..5073)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(5882..6964)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     6926..6945
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(6965..7042)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     7248..7267
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
       61 tgtttaactt taagaaggag atatacatat gtcgtactac catcaccatc accatcacga
      121 ttacgatatc ccaacgaccg aaaacctgta ttttcagggc gccatgggat cctcccagcc
      181 acgcaaaaaa atctttaaac ccgaggagct acgccaggca cttatgccaa ctctagaagc
      241 actctatcga caggacccag agtctttgcc ttttcgtcag cctgtagatc ctcagctcct
      301 aggaatccca gattattttg atatagtgaa gaatcctatg gacctttcta ccatcaaacg
      361 aaagctggac acagggcaat atcaagaacc ctggcagtat gtggatgatg tctggcttat
      421 gttcaacaat gcgtggctat ataatcgtaa aacgtcccgt gtatataaat tttgcagtaa
      481 acttgcagag gtctttgaac aagaaattga ccctgtcatg cagtctcttg gatattgctg
      541 tggacgaaag tatgagttct ccccacagac tttgtgctgt tacggaaagc agctgtgtac
      601 aattcctcgt gatgcagcct actacagcta tcagaatagg tatcatttct gtgagaagtg
      661 tttcacagag atccagggcg agaatgtgac cctgggtgac gacccttccc aacctcagac
      721 gacaatttcc aaggatcaat ttgaaaagaa gaaaaatgat accttagatc ctgaaccttt
      781 tgttgactgc aaagagtgtg gccggaagat gcatcagatt tgtgttctac actatgacat
      841 catttggcct tcaggttttg tgtgtgacaa ctgtttgaag aaaactggca gacctcggaa
      901 agaaaacaaa ttcagtgcta agaggctgca gaccacacga ttgggaaacc acttagaaga
      961 cagagtgaat aagtttttgc ggcgccagaa tcaccctgaa gctggggagg tttttgtcag
     1021 agtggtggcc agctcagaca agactgtgga ggtcaagccg ggaatgaagt caaggtttgt
     1081 ggattctgga gagatgtcgg aatctttccc atatcgtacc aaagcactct ttgcttttga
     1141 ggagatcgat ggagtcgatg tgtgcttttt tgggatgcat gtgcaagaat acggctctga
     1201 ttgcccccca ccaaatacaa ggcgtgtata catatcttat ctggacagta ttcatttctt
     1261 ccggccccgc tgcctccgga cagctgttta ccatgagatc ctcatcggat atctcgagta
     1321 tgtgaagaaa ttggggtatg tgacaggaca tatttgggcc tgtcccccaa gtgaaggaga
     1381 tgactatatc tttcattgcc acccccctga ccagaaaatc cccaaaccaa aacgactaca
     1441 ggagtggtac aagaagatgc tggacaaggc gtttgcagag aggatcatta acgactataa
     1501 ggacatcttc aaacaagcga acgaagacag gctcacgagt gccaaggagt tgccctattt
     1561 tgaaggagat ttctggccta atgtgttgga agaaagcatt aaggaatctg gaggatcagg
     1621 atcgcagaag ctgtatgcca ccatggagaa gcacaaggag gtattctttg tgattcatct
     1681 gcatgctggg cctgttatca gcactcagcc ccccatcgtg gaccctgatc ctctgcttag
     1741 ctgtgacctc atggatgggc gagatgcctt cctcaccctg gccagagaca agcactggga
     1801 attctcttcc ttacgccgct ccaaatggtc cactctgtgc atgctggtgg agctgcacac
     1861 acagggccag gaccgctttg tttatacctg caatgagtgc aaacaccatg tggaaacacg
     1921 ctggcactgc actgtgtgtg aggactatga cctttgtatc aattgctaca acacaaagag
     1981 ccacacccat aagatggtga agtgggggct aggcctagat taataggtcg acgagctcac
     2041 tagtcgcggc cgctttcgaa tctagagcct gcagtctcga gcaccaccac caccaccact
     2101 gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc
     2161 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag
     2221 gaggaactat atccggattg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     2281 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     2341 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     2401 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     2461 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     2521 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     2581 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     2641 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     2701 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     2761 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     2821 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     2881 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     2941 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     3001 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     3061 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     3121 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     3181 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     3241 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     3301 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     3361 cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa
     3421 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     3481 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     3541 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     3601 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     3661 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     3721 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     3781 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     3841 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     3901 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     3961 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     4021 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     4081 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     4141 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     4201 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     4261 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     4321 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     4381 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     4441 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     4501 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     4561 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     4621 agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     4681 tcacaccgca atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     4741 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc
     4801 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     4861 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca
     4921 gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc
     4981 cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc
     5041 catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct
     5101 gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga
     5161 tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg
     5221 gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg
     5281 tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg
     5341 cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt
     5401 tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga
     5461 ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag
     5521 cacgatcatg cgcacccgtg gggccgccat gccggcgata atggcctgct tctcgccgaa
     5581 acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac
     5641 cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac
     5701 ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc
     5761 ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa
     5821 gggcatcggt cgagatcccg gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg
     5881 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     5941 acgcgcgggg agaggcggtt tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg
     6001 agacgggcaa cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt
     6061 ccacgctggt ttgccccagc aggcgaaaat cctgtttgat ggtggttaac ggcgggatat
     6121 aacatgagct gtcttcggta tcgtcgtatc ccactaccga gatatccgca ccaacgcgca
     6181 gcccggactc ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca
     6241 tcgcagtggg aacgatgccc tcattcagca tttgcatggt ttgttgaaaa ccggacatgg
     6301 cactccagtc gccttcccgt tccgctatcg gctgaatttg attgcgagtg agatatttat
     6361 gccagccagc cagacgcaga cgcgccgaga cagaacttaa tgggcccgct aacagcgcga
     6421 tttgctggtg acccaatgcg accagatgct ccacgcccag tcgcgtaccg tcttcatggg
     6481 agaaaataat actgttgatg ggtgtctggt cagagacatc aagaaataac gccggaacat
     6541 tagtgcaggc agcttccaca gcaatggcat cctggtcatc cagcggatag ttaatgatca
     6601 gcccactgac gcgttgcgcg agaagattgt gcaccgccgc tttacaggct tcgacgccgc
     6661 ttcgttctac catcgacacc accacgctgg cacccagttg atcggcgcga gatttaatcg
     6721 ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca
     6781 acgactgttt gcccgccagt tgttgtgcca cgcggttggg aatgtaattc agctccgcca
     6841 tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc
     6901 gggaaacggt ctgataagag acaccggcat actctgcgac atcgtataac gttactggtt
     6961 tcacattcac caccctgaat tgactctctt ccgggcgcta tcatgccata ccgcgaaagg
     7021 ttttgcgcca ttcgatggtg tccgggatct cgacgctctc ccttatgcga ctcctgcatt
     7081 aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca
     7141 tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg
     7201 aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg
     7261 atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg
     7321 tagaggatcg agatctcgat cccgcgaaat
//
