LOCUS       sequence-211131-       12363 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral Mds1-Evi-1-Flag with dual BFP and puro markers .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12363)
  TITLE     Perkins lab unpublished plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pKAM-ME-Flag-BFP
FEATURES             Location/Qualifiers
     source          1..12363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          136..1074
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1031..1051
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4802..4825
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        4841..5338
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     5271..5290
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5378..5974
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5378..5397)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5874..5894
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             5993..6046
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6047..6748
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     LTR             6830..7463
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7591..7607)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7591..7607)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7604..7626)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7615..7631
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7639..7645,7646..7663,7664..7669))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7684..7705
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7822..7839)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7993..8581)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8073..8092)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8752..9543,9544..9612))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9375..9394
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9613..9717)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9765..9899
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9802..9821)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9856..9875
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10067..10700
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10747..10872
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11369..11602
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11787..11831
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    12094..12209
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        join(12287..12363,1..135)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
       61 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
      121 gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt
      181 tatggccctt gcgtgccttg aattacttcc acctggctgc agtacgtgat tcttgatccc
      241 gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg
      301 cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg
      361 caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga
      421 cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac
      481 actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca
      541 tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa
      601 gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg
      661 gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct
      721 gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc
      781 acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac
      841 cgggcgccgt ccaggcacct cgattagttc tcgagctttt ggagtacgtc gtctttaggt
      901 tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt
      961 aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct
     1021 tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtgtcg
     1081 tgaggatccc agcatgagat ccaaaggcag ggcaaggaaa ctggccacaa gtaatgagtg
     1141 tgcctatggc aactatcctg aaataccttt ggaagaaatg ccagatgctg atgcagatgg
     1201 gataaccagt gttccctccc tccacattca agagccatgc tctcctgcga cgtccagtga
     1261 gtcatttact cctaaggagg gctcgccata caaagctccc atctacatcc ctgatgacat
     1321 ccctattcct gatgagtttg agcttcgaga gtcaactatg cctggagcag gacttggaat
     1381 atggaccaaa aggaagattg aaataggcga aaagtttggg ccatacatgg gagagcagag
     1441 atcagacctg aaagattcca gctatggatg ggagatctta gatgagtttt gcaatgtgaa
     1501 gttctgcata gatgccagtc aaccagatgt aggaagctgg ctcaagtaca tcagattcgc
     1561 tggctgctat gatcagcaca accttgttgc atgccagata aatgatcaga tattctaccg
     1621 agtagtcgca gacattgcgc ctggggaaga gctcttgctg ttcatgaaga gtgaagagga
     1681 cccgcacgaa cccatggcgc ctgacatcca cgaagaacgg cagcaccgct gtgaggactg
     1741 tgaccagctc tttgaatcca aggcagagct agccgatcac cagaagttcc catgcagcac
     1801 acctcactcg gccttctcca tggtggagga ggacttgcaa caaaacctgg agagtgagag
     1861 cgatctccga gagatccatg gcaaccagga ctgtaaggaa tgtgaccgag ttttccccga
     1921 tctgcaaagc ttggagaagc acatgctgtc acatactgag gagagggaat acaagtgtga
     1981 tcagtgtccc aaggcattta actggaagtc caatttaatt cgccaccaga tgtcacatga
     2041 cagtggaaag cactatgagt gtgaaaactg tgccaaggtt ttcacggacc ctagcaacct
     2101 tcagcgacac attcgatctc agcatgttgg tgcccgggct catgcttgcc ccgagtgtgg
     2161 taaaacattt gccacttcgt caggcctcaa acagcacaag cacatccaca gcagtgtgaa
     2221 gccctttatc tgtgaggtct gccataaatc ctatactcag ttttcaaacc tttgtcgtca
     2281 taagcgcatg catgctgatt gcagaaccca aatcaagtgc aaagactgtg gacaaatgtt
     2341 cagcactacg tcttccttaa ataaacacag gaggttttgt gagggcaaga accattttgc
     2401 ggcaggtgga ttttttggcc aaggcatttc acttcctgga accccagcta tggataaaac
     2461 gtccatggtt aatatgagtc atgccaaccc gggcctggct gactattttg gcaccaatag
     2521 gcatcctgct ggtcttacct ttccaacagc tcctggattt tcctttagct ttcctggtct
     2581 gtttccttcg ggcttatacc acaggcctcc tttgataccc gctagtcctc ctgttaaagg
     2641 attatcaagt accgaacagt caaacaaatg tcaaagtccc ctcttgacac atcctcagat
     2701 actgccagct acacaggata ttttgaaggc actatctaaa cacccacctg taggggacaa
     2761 taagccagtg gaactgctgc ccgagaggtc ctctgaagag aggccccttg agaaaatcag
     2821 tgaccagtca gagagtagtg accttgatga tgtcagtaca ccaagtggca gtgacctgga
     2881 aacaacctcg ggctctgatc tggaaagtga ccttgaaagt gataaagaga aatgtaaaga
     2941 aaatggtaaa atgttcaaag acaaagtaag ccctctgcag aatctggctt cgataactaa
     3001 taagaaagaa cacaacaatc attccgtttt ctcagcatct gtagaggagc aaagtgccgt
     3061 gtcaggagct gtgaatgatt ctataaaggc tattgcgtct attgctgaaa aatactttgg
     3121 ttctacagga ctggtggggc tgcaagacaa aaaagttgga gcgttacctt acccttccat
     3181 gtttcccctc ccgttttttc cagcattctc tcaatcaatg tacccatttc ctgatagaga
     3241 cttgaggtcg ttacctttga aaatggagcc ccaatcacca agtgaagtta agaaactgca
     3301 gaagggaagc tctgagtccc cttttgacct caccactaag agaaaggatg agaagccctt
     3361 gacttcaggc ccctcgaagc cttcaggaac accagccaca agccaagacc agcccctgga
     3421 tctaagtatg ggcagtaggg gtagagccag tgggacaaag ttgactgagc ctcgaaaaaa
     3481 ccatgtgttt ggggaaaaga aaggaagcaa catggatact aggccatctt cagatggctc
     3541 cttgcagcat gccagaccca ctcccttctt catggacccc atttatagag tagagaaaag
     3601 aaagttaact gacccgcttg aagctttgaa agaaaaatac ttgagacctt ctccaggatt
     3661 cttgtttcac ccgcaaatgt cagcaattga gaacatggca gaaaagctgg aaagcttcag
     3721 cgccctcaaa cctgaggcca gcgagctcct gcagtccgtg ccctccatgt tcagcttccg
     3781 agctcctccc aacaccctgc cagagaacct gctgcggaag gggaaagagc gctacacctg
     3841 caggtactgt ggcaagatat ttccaaggtc tgcgaaccta acacggcact tgagaaccca
     3901 cacaggagag caaccttaca gatgcaaata ctgtgataga tcattcagca tttcttccaa
     3961 cctgcagcga catgtgcgca acatccacaa caaggagaag ccatttaagt gtcatttatg
     4021 tgacagatgt tttggtcaac aaaccaatct tgacagacac ctgaagaaac atgagaacgg
     4081 caacatgtct gggacggcaa cgtcctcgcc tcactcagag ctagaaagcg caggcgcaat
     4141 cctggatgac aaagaagatg cgtactttac agagatccgc aatttcatcg ggaacagcaa
     4201 ccatggtagc cagtctcctc ggaacatgga agagaggatg aatggcagtc acttcaagga
     4261 taaaaaggct ttggcaacca gccaaaattc agatttattg gatgatgaag aagtagaaga
     4321 tgaggtgttg ttggatgagg aggatgaaga caatgatatt cctggaaagc ccagaaagga
     4381 gctaggggtg actcgtttag acgaggagat cccggaggat gactacgaag aagctggtgc
     4441 cctggagatg agctgtaagg cgtccccggt gaggtataaa gaggaagact ataaatctgg
     4501 cctttctgct ctagatcaca taaggcactt cacagatagc ctcaaaatga gggaaatgga
     4561 agagaatcaa tacactgacg ctgagctgtc ctccattagt tcttctcatg tgccagagga
     4621 gcttaaacag acgttacaca gaaagtccaa atcacaggca tatgctatga tgttgtcact
     4681 gtctgacaag gattccctcc atcccacctc ccacagttcc tccaacgtgt ggcacagcat
     4741 ggcaagggct gcagcagaat ccagtgccat ccagtccata agccatgtag gcagcggcgt
     4801 agattacaag gatgacgacg ataagtaggc gaattctacc gggtagggga ggcgcttttc
     4861 ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt
     4921 ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt
     4981 ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg
     5041 cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag
     5101 atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag
     5161 ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggggctcag
     5221 gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac
     5281 gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac
     5341 ctgcatccat ctagatctcg agcagctgaa gcttaccatg accgagtaca agcccacggt
     5401 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     5461 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     5521 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     5581 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     5641 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     5701 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     5761 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     5821 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     5881 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     5941 gcgcacctgg tgcatgaccc gcaagcccgg tgccggcggc gggtccggag gagagggcag
     6001 aggaagtctc ctaacatgcg gtgacgtgga ggagaatcct ggcccaatga gcgagctgat
     6061 taaggagaac atgcacatga agctgtacat ggagggcacc gtggacaacc atcacttcaa
     6121 gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt
     6181 ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctactagct tcctctacgg
     6241 cagcaagacc ttcatcaacc acacccaggg catccccgac ttcttcaagc agtccttccc
     6301 tgagggcttc acatgggaga gagtcaccac atacgaggac gggggcgtgc tgaccgctac
     6361 ccaggacacc agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa
     6421 cttcacatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccttcaccga
     6481 gacgctgtac cccgctgacg gcggcctgga aggcagaaac gacatggccc tgaagctcgt
     6541 gggcgggagc catctgatcg caaacatcaa gaccacatat agatccaaga aacccgctaa
     6601 gaacctcaag atgcctggcg tctactatgt ggactacaga ctggaaagaa tcaaggaggc
     6661 caacaacgag acctacgtcg agcagcacga ggtggcagtg gccagatact gcgacctccc
     6721 tagcaaactg gggcacaagc ttaattgagc ggccgctagg tacctttaag accaatgact
     6781 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa agaggggact ggaagggcta
     6841 attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
     6901 ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga
     6961 tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag
     7021 aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg
     7081 ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg
     7141 gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt
     7201 ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa
     7261 gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct
     7321 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     7381 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     7441 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     7501 acttgcaaag aaatgaatat cagagagtga gaggccttga cattgctagc gtttaccgtc
     7561 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     7621 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     7681 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     7741 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     7801 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     7861 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     7921 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7981 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     8041 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     8101 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     8161 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     8221 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     8281 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     8341 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     8401 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     8461 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8521 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8581 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8641 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8701 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     8761 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     8821 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     8881 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     8941 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     9001 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     9061 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     9121 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     9181 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     9241 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     9301 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     9361 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     9421 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     9481 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     9541 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9601 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     9661 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     9721 atttccccga aaagtgccac ctgacgtcga cggatcggga gatcaacttg tttattgcag
     9781 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     9841 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca
     9901 actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca
     9961 ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat
    10021 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt
    10081 cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
    10141 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
    10201 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
    10261 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
    10321 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
    10381 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca
    10441 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
    10501 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
    10561 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
    10621 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
    10681 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
    10741 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
    10801 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
    10861 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
    10921 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
    10981 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
    11041 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
    11101 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
    11161 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggccgc
    11221 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
    11281 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
    11341 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
    11401 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
    11461 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
    11521 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
    11581 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
    11641 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
    11701 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
    11761 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
    11821 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
    11881 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
    11941 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
    12001 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
    12061 ttcgattagt gaacggatct cgacggtatc gcctttaaaa gaaaaggggg gattgggggg
    12121 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
    12181 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
    12241 gtttatcgat gaggcccttt cgtcttcact cgaggtgccc gtcagtgggc agagcgcaca
    12301 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga
    12361 agg
//
