LOCUS       sequence-211155-       11827 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C. thermophilum DASH/Dam1 complex (construct used for cryo-EM 
            structure determination).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11827)
  AUTHORS   Jenni S, Harrison SC
  TITLE     Structure of the DASH/Dam1 complex shows its role at the yeast 
            kinetochore-microtubule interface.
  JOURNAL   Science. 2018 May 4;360(6388):552-558. doi: 10.1126/science.aar6436.
  PUBMED    29724956
REFERENCE   2  (bases 1 to 11827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2018-05-4- 4"; volume: "360"; issue: 
            "6388"; pages: "552-558"
COMMENT     Sequence Label: jk089_h12
FEATURES             Location/Qualifiers
     source          1..11827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    52..71
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    192..211
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        284..302
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             334..356
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             538..561
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             577..600
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             616..639
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     terminator      676..723
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     protein_bind    796..815
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     primer_bind     837..853
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    904..923
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        996..1014
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             1046..1068
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      1445..1492
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     protein_bind    1565..1584
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    1673..1692
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        1765..1783
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             1815..1837
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2562..2579
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      2616..2663
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     protein_bind    2736..2755
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    2844..2863
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        2936..2954
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             2986..3008
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      3220..3267
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     protein_bind    3340..3359
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    3448..3467
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        3540..3558
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             3590..3612
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      3839..3886
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     protein_bind    3959..3978
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    4091..4110
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     primer_bind     complement(4181..4203)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4289..4428
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4344..4363
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4443..4460)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4614..5202)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4694..4713)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(5233..5272)
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     promoter        complement(6137..6228)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    6276..6309
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(6489..7283)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6600..6619)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7210..7229
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     protein_bind    8008..8027
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    8156..8175
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        8248..8266
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             8298..8320
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      8580..8627
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     protein_bind    8700..8719
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     primer_bind     8741..8757
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8808..8827
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        8900..8918
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             8950..8972
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      9217..9264
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     protein_bind    9337..9356
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    9445..9464
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        9537..9555
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             9587..9609
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      9935..9982
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     protein_bind    10055..10074
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    10163..10182
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        10255..10273
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             10305..10327
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      10824..10871
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     protein_bind    10944..10963
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    11052..11071
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        11144..11162
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             11194..11216
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      11491..11538
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     protein_bind    11611..11630
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    11743..11762
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    11794..11827
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gggggtaccg cggccgcgta gaggagatgt aggtgttggc accggccggc caattgtgag
       61 cgctcacaat tactcgagta tgcataacta taacggtcct aaggtagcga cctaggttct
      121 agacttcatt tccttctcca gattaactca attccagcat ttagaaccct accaagtatc
      181 gatggccggc caattgtgag cgctcacaat ttcgagctct gagccctact gtcgagaaat
      241 atgtatctcg cactcgagtg gcgcgccctc gatcccgcga aattaatacg actcactata
      301 gggagaccac aacggtttcc ctctagaaat aattttgttt aactttaaga aggagatata
      361 catatgtcgt atttcgagca gcagcgccag gcgctgatag aagagattgc catgaacttc
      421 gagcatgtcc tggccaacat aaacaaacta aaccggtcgc tcgaggccgt tatcgctgta
      481 ggaaatgagt tctcatctgt cgaggcgctc tggtcgcaat tcgaaaacgt gatgtcttgg
      541 agccatccgc agtttgaaaa aggcgccatg accggttggt cccacccgca gttcgaaaaa
      601 cgtagcgcgg gcagctggag tcatccgcaa ttcgaaaaat aattaaccta ggctgctgcc
      661 accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt
      721 ttgctgaaag gcgccggcgg cggccgcggc cggccaccag cagcttgcca gctctttcag
      781 tatcatggag cccataattg tgagcgctca caattactcg agtcccgggg gatcctgtaa
      841 aacgacggcc agtgaattct agagcaataa cccatcaatc tgtacgcaca tcgatggccg
      901 gccaattgtg agcgctcaca atttcgagct ctgagcccta ctgtcgagaa atatgtatct
      961 cgcactcgag tggcgcgccc tcgatcccgc gaaattaata cgactcacta tagggagacc
     1021 acaacggttt ccctctagaa ataattttgt ttaactttaa gaaggagata tacatatgga
     1081 agcgcgcgaa gcagcccttc gcaaggagct cgagggcgtt cgcaagatca acgaggttat
     1141 cgagggcatg atcgggacgc tggagcgggc taaggggaat atgggtaccg tctcccaaac
     1201 cgtcaccaac gccacaaccc tgctcaacac ctggacgcgc atgctctcgc agaccgagca
     1261 caaccagcgc ctgatcctca acccggaatg gaagggtgcc acgcaggatc tgctcgagtt
     1321 ggaagcggaa gagcgtagaa gacaagagga ggttgagcgt cgcgctgcgg aggcagagcg
     1381 tcgtcgggaa gaggctcgtc ggaaggccta attaacctag gctgctgcca ccgctgagca
     1441 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg
     1501 cgccggcggc ggccgcggcc ggccaccagc agcttgccag ctctttcagt atcatggagc
     1561 ccataattgt gagcgctcac aattactcga gtcccgggaa gcttcgccag ggttttccaa
     1621 gtcgatcacc tacagattgc tagagtatgc ctttccagat cgatggccgg ccaattgtga
     1681 gcgctcacaa tttcgagctc tgagccctac tgtcgagaaa tatgtatctc gcactcgagt
     1741 ggcgcgccct cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc
     1801 cctctagaaa taattttgtt taactttaag aaggagatat acatatgtcg ttattatcag
     1861 cacacctgga gcagatctcc atctcctgcc aagggatcga cagtctcccc ttcccccctc
     1921 ccaaaatctt caccaacgct cttctctcca atcccgacat cacctctcta atccgtgaca
     1981 ccgaagccca cgagcgcgcc ctcttctctg tcccacctcc cccaccccgc caaaccactc
     2041 ttactgctga acaacaacaa caacagaagc cttccaatcg ccggcaaaca gtcttcaatg
     2101 tcaccggcgg cgagatcagg acaggtggcg taggcagcgc atcaaccgcc cgccgtaaca
     2161 cggcggtggc ggccgtgctg ggcggcgacc tgcacgcgca gatcatgcgg gggactcgag
     2221 ccaggccggg acagcagcct gggtctgggg atatagatat ggaggtgctg ttgagagggg
     2281 ttgagaagtt gtgcgctgta tacccgctgc cgggggcgtt ggagagggtt ccggtgatca
     2341 ggcagaagtg gcaggcgcag agtaatacgt tggcgtacta tgaggcgaag attgctgaac
     2401 agcaggagat gttggatcgg attgcgcagg agaggatgat gaatgatggc gatggggatg
     2461 tggagatgga ggatgtagaa gaggttggga tgacggagga ggatttgagg agggaggagg
     2521 aggaggtaag ggagttggat aaaaggaaga gggatttgca gcatcaccat catcaccact
     2581 aattaaccta ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta
     2641 aacgggtctt gaggggtttt ttgctgaaag gcgccggcgg cggccgcggc cggccaccag
     2701 cagcttgcca gctctttcag tatcatggag cccataattg tgagcgctca caattactcg
     2761 agtcccgggg gtaccttctc attcccggat cacatcacta tctctgtaat atggcgttct
     2821 tctgattcca tcgatggccg gccaattgtg agcgctcaca atttcgagct ctgagcccta
     2881 ctgtcgagaa atatgtatct cgcactcgag tggcgcgccc tcgatcccgc gaaattaata
     2941 cgactcacta tagggagacc acaacggttt ccctctagaa ataattttgt ttaactttaa
     3001 gaaggagata tacatatgaa cgcatttgag ccagcatttg ccgagctcgc cgatgctgtg
     3061 gccgacctcg aggccaacat gatgcacttt cagttgatgc acgaatcact ggcccggttc
     3121 agcgaaagct tcgccagttt tctctacggt ctgaacatga acgcgttctg tgttgatttc
     3181 ccataattaa cctaggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc
     3241 tctaaacggg tcttgagggg ttttttgctg aaaggcgccg gcggcggccg cggccggcca
     3301 ccagcagctt gccagctctt tcagtatcat ggagcccata attgtgagcg ctcacaatta
     3361 ctcgagtccc ggggagattt gtaagagctg ttagttacgt cgctacccaa tgtcgttagt
     3421 gtaggttctg agcatcgatg gccggccaat tgtgagcgct cacaatttcg agctctgagc
     3481 cctactgtcg agaaatatgt atctcgcact cgagtggcgc gccctcgatc ccgcgaaatt
     3541 aatacgactc actataggga gaccacaacg gtttccctct agaaataatt ttgtttaact
     3601 ttaagaagga gatatacata tggctgtcaa ggcgaggcaa ttggcccatc tccactcaca
     3661 gctgacccag ctttctcaca acttggccac cacagaaaac ttgatgcgta tgacagcagt
     3721 acaggctgaa gctatgcgag gtcttggaag ctggcatgca ggacttttca tggctgcaag
     3781 caaggttctt ggagaggagt cgtaattaac ctaggctgct gccaccgctg agcaataact
     3841 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggcgccgg
     3901 cggcggccgc ggccggccac cagcagcttg ccagctcttt cagtatcatg gagcccataa
     3961 ttgtgagcgc tcacaattac tcgagtcccg ggctcgagag atccggctgc taacaaaccc
     4021 gcttaaggaa gatggccaac ttatttacat aaggctgctg ccaccgctga gcaataacta
     4081 gcggcgcgcc aattgtgagc gctcacaatt cgccggcggt ttaaacccat ctaattggga
     4141 attccccctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     4201 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     4261 cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg
     4321 gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccattg
     4381 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct
     4441 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     4501 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     4561 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     4621 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     4681 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     4741 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     4801 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     4861 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     4921 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     4981 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     5041 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     5101 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     5161 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     5221 tctaccggtc cgtccaaaaa aaaaggctcc aaaaggagcc tttaattgta tcggtttacc
     5281 aatgcttaat cagtgaagcc cctatttcag caatctgtct atttcgttcg tccatagttg
     5341 cctgactacc cgtcgtgtag ataactacga tacgggaagg cttaccatct ggtccaagtg
     5401 cggcaatgat accccgtgaa ccacgctctc ctgccccaga tttatcagca ataaaccagc
     5461 cagcaggaag tgccgaacgc agtagtggtc ctgcaacttt atccgcttca agccagtcta
     5521 ttaattgttg ccgtgaagcc agagtaagta gttcgccagt taatagtttg cgtaatgttg
     5581 ttgccattgc cgcaggcatt gtggtgtctc gctcgtcgtt tggtatggct tcattcagtt
     5641 ctggttccca acggtcaagg cgagttacat ggtctcccat attgtgcaaa aaagcggtta
     5701 attccttcgg tccaccgatg gttgtcagaa gtaagttcgc cgcagtgtta tcactcatcg
     5761 ttatggcggc actacataat tctcttaccg tcattccgtc cgtaaggtgc ttttctgtca
     5821 ctggggagta ttcaaccaag tcattctgcg aatagtgtat tcgtcgtccg agttgctctt
     5881 gccccgcatc tacacgggat aatactgctc cacatagcag aactttgaaa gtgctcatca
     5941 ttggaaaacg ctcttcaggg cgaaaactct caaggatttt accgctattc aagtccagtt
     6001 ctatgtatcc cactcgtgcc cccaattggt cctctgcgtc ttttactttc acgagcgttt
     6061 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     6121 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     6181 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggaattaa
     6241 aaaaaagccc gctcattagg cgggctacta gtcagataac ttcgtataat gtatgctata
     6301 cgaagttatg ggtacgtagt gggcgaagaa ctccagcatg agatccccgc gctggaggat
     6361 catccagccg gcgtcccgga aaacgattcc gaagcccaac ctttcataga aggcggcggt
     6421 ggaatcgaaa tctcgtgatg gcaggttggg cgtcgcttgg tcggtcattt cgaaccccag
     6481 agtcccgctc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga
     6541 gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca
     6601 atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag
     6661 tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca
     6721 tgtgtcacga cgagatcctc gccgtcgggc atgcgcgcct tgagcctggc gaacagttcg
     6781 gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc
     6841 atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc
     6901 ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga
     6961 gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt
     7021 cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac
     7081 gatagccgcg ctgcctcgtc ctgcagttca ttcagggcac cggacaggtc ggtcttgaca
     7141 aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt
     7201 gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg
     7261 tgcaatccat cttgttcaat catgcgaaac gatcctcatc ctgtctcttg atcagatctt
     7321 gatcccctgc gccatcagat ccttggcggc aagaaagcca tccagtttac tttgcagggc
     7381 ttcccaacct taccagaggg cgccccagct ggcaattccg gttcgcttgc tgtccataaa
     7441 accgcccagt ctagctatcg ccatgtaagc ccactgcaag ctacctgctt tctctttgcg
     7501 cttgcgtttt cccttgtcca gatagcccag tagctgacat tcatccgggg tcagcaccgt
     7561 ttctgcggac tggctttcta cgtgttccgc ttcctttagc agcccttgcg ccctgagtgc
     7621 ttgcggcagc gtgaagctaa ttctgtcagc cgttaagtgt tcctgtgtca ctgaaaattg
     7681 ctttgagagg ctctaagggc ttctcagtgc gttacatccc tggcttgttg tccacaaccg
     7741 ttaaacctta aaagctttaa aagccttata tattcttttt tttcttataa aacttaaaac
     7801 cttagaggct atttaagttg ctgatttata ttaattttat tgttcaaaca tgagagctta
     7861 gtacgtgaaa catgagagct tagtacgtta gccatgagag cttagtacgt tagccatgag
     7921 ggtttagttc gttaaacatg agagcttagt acgttaaaca tgagagctta gtacgtgaaa
     7981 catgagagct tagcccgggg gccggccaat tgtgagcgct cacaattact cgagtatcta
     8041 tgtcgggtgc ggagaaagag gtaatgaaat ggcacctagg ttctagactt catttccttc
     8101 tccagattaa ctcaattcca gcatttagaa ccctaccaag tatcgatggc cggccaattg
     8161 tgagcgctca caatttcgag ctctgagccc tactgtcgag aaatatgtat ctcgcactcg
     8221 agtggcgcgc cctcgatccc gcgaaattaa tacgactcac tatagggaga ccacaacggt
     8281 ttccctctag aaataatttt gtttaacttt aagaaggaga tatacatatg gagagccctc
     8341 acgagcatca gcagaacttg ctgctgtcgc gtatcatcac gaatgtggag aagctcaacg
     8401 aggccatcat ggtgatgaac aagacccttc aggaaatcaa catccagaac atgaacattg
     8461 aacttgtggc ccagatgttt aagaactatc aatccaacgt cttgttccat ctggaggcta
     8521 ccgataactt gaaggatccg gcataattaa cctaggctgc tgccaccgct gagcaataac
     8581 tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggcgccg
     8641 gcggcggccg cggccggcca ccagcagctt gccagctctt tcagtatcat ggagcccata
     8701 attgtgagcg ctcacaatta ctcgagtccc gggggatcct gtaaaacgac ggccagtgaa
     8761 ttctagagca ataacccatc aatctgtacg cacatcgatg gccggccaat tgtgagcgct
     8821 cacaatttcg agctctgagc cctactgtcg agaaatatgt atctcgcact cgagtggcgc
     8881 gccctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct
     8941 agaaataatt ttgtttaact ttaagaagga gatatacata tgctctctcc actcgagcaa
     9001 gaagtgttgg atgagtatga gcggctatct gagaacatga agaagttggc tgtgctcttg
     9061 gatgagcttg ccagtgcccc agcaactgag attttagatg ggctgcgcga gttggagcgt
     9121 aagactagcc tcgtgttcac attgcttaag gctagtgtgt acagcatcgt gttgcagcag
     9181 taattaacct aggctgctgc caccgctgag caataactag cataacccct tggggcctct
     9241 aaacgggtct tgaggggttt tttgctgaaa ggcgccggcg gcggccgcgg ccggccacca
     9301 gcagcttgcc agctctttca gtatcatgga gcccataatt gtgagcgctc acaattactc
     9361 gagtcccggg aagcttcgcc agggttttcc aagtcgatca cctacagatt gctagagtat
     9421 gcctttccag atcgatggcc ggccaattgt gagcgctcac aatttcgagc tctgagccct
     9481 actgtcgaga aatatgtatc tcgcactcga gtggcgcgcc ctcgatcccg cgaaattaat
     9541 acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta
     9601 agaaggagat atacatatgt cgccggctct gctggcgcgg gttaacgaga agaaggctga
     9661 gctcgagaac ctgaaggagc ttcgcgatct cagcgcagct gttgctgccc agatggaggc
     9721 acttgagcag aagctctcta ctctctctag cgggactgag gccattgcca cggtgttggc
     9781 taattggcat aatgtgctac gcgccatcag catggcatcg gccaagattc ccgaaccaaa
     9841 ggaggaaact gaggagaaca cggttcctct tccccagact ctggtccgga ttcccaccta
     9901 attaacctag gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa
     9961 acgggtcttg aggggttttt tgctgaaagg cgccggcggc ggccgcggcc ggccaccagc
    10021 agcttgccag ctctttcagt atcatggagc ccataattgt gagcgctcac aattactcga
    10081 gtcccggggg taccttctca ttcccggatc acatcactat ctctgtaata tggcgttctt
    10141 ctgattccat cgatggccgg ccaattgtga gcgctcacaa tttcgagctc tgagccctac
    10201 tgtcgagaaa tatgtatctc gcactcgagt ggcgcgccct cgatcccgcg aaattaatac
    10261 gactcactat agggagacca caacggtttc cctctagaaa taattttgtt taactttaag
    10321 aaggagatat acatatgtct tacgctgatt gtgtctgctc tcttcggacc tccctcgcct
    10381 tcctcgaatc ctcggtcgca accctcgaca acggcgtcca agacttcccc cgcctgtgcc
    10441 acgtcctcag aaccgtgcgt cactacgaac taatccccca gacaaccctc gccgccgcag
    10501 aagcttccct ccgcgacgaa atcggcccgt ttatccagct ccttctcgac cgtgccgaga
    10561 agcatctaga tcgccaggca aggaggatag aaaccctcaa ggcgcgcgca gaactcaacg
    10621 cagggagact gtcgcagtac tctggcgacg gacacaacaa tgggaaattc tctggtcagg
    10681 ggatggattt caggaagagt cgtccgctga atggggaggc ggctctgagg gccaaagttg
    10741 taaggcagag gaaggaggcg ttaaagtata gtgttgagag gctggagtaa ttaacctagg
    10801 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
    10861 ggggtttttt gctgaaaggc gccggcggcg gccgcggccg gccaccagca gcttgccagc
    10921 tctttcagta tcatggagcc cataattgtg agcgctcaca attactcgag tcccggggag
    10981 atttgtaaga gctgttagtt acgtcgctac ccaatgtcgt tagtgtaggt tctgagcatc
    11041 gatggccggc caattgtgag cgctcacaat ttcgagctct gagccctact gtcgagaaat
    11101 atgtatctcg cactcgagtg gcgcgccctc gatcccgcga aattaatacg actcactata
    11161 gggagaccac aacggtttcc ctctagaaat aattttgttt aactttaaga aggagatata
    11221 catatgaccc tgacggaaga gcttgaaaag ctcgagcagc agattaccct cacgctgcaa
    11281 gagatcgact ccaactttgc caaagcccac cgcatcgtca ctacgagcat cctgccactc
    11341 gtcgagcagt atggcgagca ctcacgcgca gtgtgggagg cgaccaagtt ttggaaacaa
    11401 ttcttcgaag cctccgcaaa cgtctctctc tccggctacg aagagctagt tgactaatta
    11461 acctaggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg
    11521 gtcttgaggg gttttttgct gaaaggcgcc ggcggcggcc gcggccggcc accagcagct
    11581 tgccagctct ttcagtatca tggagcccat aattgtgagc gctcacaatt actcgagtcc
    11641 cgggctcgag agatccggct gctaacaaac ccgcttaagg aagatggcca acttatttac
    11701 ataaggctgc tgccaccgct gagcaataac tagcggcgcg ccaattgtga gcgctcacaa
    11761 ttcgccggcg gtttaaaccc atgtgcctgg cagataactt cgtataatgt atgctatacg
    11821 aagttat
//
