LOCUS       sequence-211156-       10780 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Cas9 fusion to HE (HDR-enhancer) domain of human CtIP 
            protein and Geminin degron.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10780)
  AUTHORS   Charpentier M, Khedher AHY, Menoret S, Brion A, Lamribet K, 
            Dardillac E, Boix C, Perrouault L, Tesson L, Geny S, De Cian A, 
            Itier JM, Anegon I, Lopez B, Giovannangeli C, Concordet JP
  TITLE     CtIP fusion to Cas9 enhances transgene integration by 
            homology-dependent repair.
  JOURNAL   Nat Commun. 2018 Mar 19;9(1):1133. doi: 10.1038/s41467-018-03475-7.
  PUBMED    29556040
REFERENCE   2  (bases 1 to 10780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-03-19"; volume: "9(1):1133. doi"; pages: " 
            10.1038/s41467-018-03475-7"
COMMENT     Sequence Label: pCas9-HE-Geminin
FEATURES             Location/Qualifiers
     source          1..10780
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        60..439
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        440..643
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     594..614
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     640..664
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      752..761
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             758..4861
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             758..4861
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             758..4861
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4874..4894
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5807..6136
                     /codon_start=1
                     /product="degron consisting of residues 1-110 of human 
                     Geminin (Zielke and Edgar, 2015)"
                     /label=Gem1 (1-110)
                     /translation="MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELS
                     AGLSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWKEVAE
                     KRRKAL"
     primer_bind     complement(6176..6195)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6220..6268
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6470..6898
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6557..6576)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6767..6788
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6907..6927)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6912..7241
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7092..7227
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7154..7173
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7308..8102
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7362..7381)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7972..7991
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8278..8399
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8315..8334)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8369..8388
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8448..8464)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8448..8464)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8461..8483)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8472..8488
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8496..8502,8503..8520,8521..8526))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8541..8562
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8679..8696)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8850..9438)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8930..8949)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9609..10400,10401..10469))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSV
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10232..10251
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10470..10574)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10649..10668)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg
       61 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
      121 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
      181 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      241 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      301 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      361 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      421 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      481 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      541 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      601 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
      661 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc
      721 cagcctccgg actctagagg atcgaaccct tgccaccatg gacaagaagt actccattgg
      781 gctcgatatc ggcacaaaca gcgtcggctg ggccgtcatt acggacgagt acaaggtgcc
      841 gagcaaaaaa ttcaaagttc tgggcaatac cgatcgccac agcataaaga agaacctcat
      901 tggcgccctc ctgttcgact ccggggagac ggccgaagcc acgcggctca aaagaacagc
      961 acggcgcaga tatacccgca gaaagaatcg gatctgctac ctgcaggaga tctttagtaa
     1021 tgagatggct aaggtggatg actctttctt ccataggctg gaggagtcct ttttggtgga
     1081 ggaggataaa aagcacgagc gccacccaat ctttggcaat atcgtggacg aggtggcgta
     1141 ccatgaaaag tacccaacca tatatcatct gaggaagaag cttgtagaca gtactgataa
     1201 ggctgacttg cggttgatct atctcgcgct ggcgcatatg atcaaatttc ggggacactt
     1261 cctcatcgag ggggacctga acccagacaa cagcgatgtc gacaaactct ttatccaact
     1321 ggttcagact tacaatcagc ttttcgaaga gaacccgatc aacgcatccg gagttgacgc
     1381 caaagcaatc ctgagcgcta ggctgtccaa atcccggcgg ctcgaaaacc tcatcgcaca
     1441 gctccctggg gagaagaaga acggcctgtt tggtaatctt atcgccctgt cactcgggct
     1501 gacccccaac tttaaatcta acttcgacct ggccgaagat gccaagcttc aactgagcaa
     1561 agacacctac gatgatgatc tcgacaatct gctggcccag atcggcgacc agtacgcaga
     1621 cctttttttg gcggcaaaga acctgtcaga cgccattctg ctgagtgata ttctgcgagt
     1681 gaacacggag atcaccaaag ctccgctgag cgctagtatg atcaagcgct atgatgagca
     1741 ccaccaagac ttgactttgc tgaaggccct tgtcagacag caactgcctg agaagtacaa
     1801 ggaaattttc ttcgatcagt ctaaaaatgg ctacgccgga tacattgacg gcggagcaag
     1861 ccaggaggaa ttttacaaat ttattaagcc catcttggaa aaaatggacg gcaccgagga
     1921 gctgctggta aagcttaaca gagaagatct gttgcgcaaa cagcgcactt tcgacaatgg
     1981 aagcatcccc caccagattc acctgggcga actgcacgct atcctcaggc ggcaagagga
     2041 tttctacccc tttttgaaag ataacaggga aaagattgag aaaatcctca catttcggat
     2101 accctactat gtaggccccc tcgcccgggg aaattccaga ttcgcgtgga tgactcgcaa
     2161 atcagaagag accatcactc cctggaactt cgaggaagtc gtggataagg gggcctctgc
     2221 ccagtccttc atcgaaagga tgactaactt tgataaaaat ctgcctaacg aaaaggtgct
     2281 tcctaaacac tctctgctgt acgagtactt cacagtttat aacgagctca ccaaggtcaa
     2341 atacgtcaca gaagggatga gaaagccagc attcctgtct ggagagcaga agaaagctat
     2401 cgtggacctc ctcttcaaga cgaaccggaa agttaccgtg aaacagctca aagaagacta
     2461 tttcaaaaag attgaatgtt tcgactctgt tgaaatcagc ggagtggagg atcgcttcaa
     2521 cgcatccctg ggaacgtatc acgatctcct gaaaatcatt aaagacaagg acttcctgga
     2581 caatgaggag aacgaggaca ttcttgagga cattgtcctc acccttacgt tgtttgaaga
     2641 tagggagatg attgaagaac gcttgaaaac ttacgctcat ctcttcgacg acaaagtcat
     2701 gaaacagctc aagaggcgcc gatatacagg atgggggcgg ctgtcaagaa aactgatcaa
     2761 tgggatccga gacaagcaga gtggaaagac aatcctggat tttcttaagt ccgatggatt
     2821 tgccaaccgg aacttcatgc agttgatcca tgatgactct ctcaccttta aggaggacat
     2881 ccagaaagca caagtttctg gccaggggga cagtcttcac gagcacatcg ctaatcttgc
     2941 aggtagccca gctatcaaaa agggaatact gcagaccgtt aaggtcgtgg atgaactcgt
     3001 caaagtaatg ggaaggcata agcccgagaa tatcgttatc gagatggccc gagagaacca
     3061 aactacccag aagggacaga agaacagtag ggaaaggatg aagaggattg aagagggtat
     3121 aaaagaactg gggtcccaaa tccttaagga acacccagtt gaaaacaccc agcttcagaa
     3181 tgagaagctc tacctgtact acctgcagaa cggcagggac atgtacgtgg atcaggaact
     3241 ggacatcaat cggctctccg actacgacgt ggatcatatc gtgccccagt cttttctcaa
     3301 agatgattct attgataata aagtgttgac aagatccgat aaaaatagag ggaagagtga
     3361 taacgtcccc tcagaagaag ttgtcaagaa aatgaaaaat tattggcggc agctgctgaa
     3421 cgccaaactg atcacacaac ggaagttcga taatctgact aaggctgaac gaggtggcct
     3481 gtctgagttg gataaagccg gcttcatcaa aaggcagctt gttgagacac gccagatcac
     3541 caagcacgtg gcccaaattc tcgattcacg catgaacacc aagtacgatg aaaatgacaa
     3601 actgattcga gaggtgaaag ttattactct gaagtctaag ctggtctcag atttcagaaa
     3661 ggactttcag ttttataagg tgagagagat caacaattac caccatgcgc atgatgccta
     3721 cctgaatgca gtggtaggca ctgcacttat caaaaaatat cccaagcttg aatctgaatt
     3781 tgtttacgga gactataaag tgtacgatgt taggaaaatg atcgcaaagt ctgagcagga
     3841 aataggcaag gccaccgcta agtacttctt ttacagcaat attatgaatt ttttcaagac
     3901 cgagattaca ctggccaatg gagagattcg gaagcgacca cttatcgaaa caaacggaga
     3961 aacaggagaa atcgtgtggg acaagggtag ggatttcgcg acagtccgga aggtcctgtc
     4021 catgccgcag gtgaacatcg ttaaaaagac cgaagtacag accggaggct tctccaagga
     4081 aagtatcctc ccgaaaagga acagcgacaa gctgatcgca cgcaaaaaag attgggaccc
     4141 caagaaatac ggcggattcg attctcctac agtcgcttac agtgtactgg ttgtggccaa
     4201 agtggagaaa gggaagtcta aaaaactcaa aagcgtcaag gaactgctgg gcatcacaat
     4261 catggagcga tcaagcttcg aaaaaaaccc catcgacttt ctcgaggcga aaggatataa
     4321 agaggtcaaa aaagacctca tcattaagct tcccaagtac tctctctttg agcttgaaaa
     4381 cggccggaaa cgaatgctcg ctagtgcggg cgagctgcag aaaggtaacg agctggcact
     4441 gccctctaaa tacgttaatt tcttgtatct ggccagccac tatgaaaagc tcaaagggtc
     4501 tcccgaagat aatgagcaga agcagctgtt cgtggaacaa cacaaacact accttgatga
     4561 gatcatcgag caaataagcg aattctccaa aagagtgatc ctcgccgacg ctaacctcga
     4621 taaggtgctt tctgcttaca ataagcacag ggataagccc atcagggagc aggcagaaaa
     4681 cattatccac ttgtttactc tgaccaactt gggcgcgcct gcagccttca agtacttcga
     4741 caccaccata gacagaaagc ggtacacctc tacaaaggag gtcctggacg ccacactgat
     4801 tcatcagtca attacggggc tctatgaaac aagaatcgac ctctctcagc tcggtggaga
     4861 cagcagggct gaccccaaga aaaagaggaa ggtgggatcc gctagcatga acatctcggg
     4921 aagcagctgt ggaagcccta actctgcaga tacatctagt gactttaaag atctgtggac
     4981 caaactaaaa gaatgtcatg atagagaagt acaaggttta caagtaaaag taaccaagct
     5041 taagcaagag cgaatcttag atgcacaaag actagaagaa ttcttcacca aaaatcaaca
     5101 gctgagggaa cagcagaaag tccttcatga agccattaaa gttttagaag atcggttaag
     5161 agcaggctta tgtgatcgct gtgcagtaac tgaagaacat atgcggaaaa aacagcaaga
     5221 gtttgaaaat atccggcagc agaatcttaa acttattaca gaacttatga atgaaaggaa
     5281 tactctacag gaagaaaata aaaagctttc tgaacaactc cagcagaaaa ttgagaatga
     5341 tcaacagcat caagcagctg agcttgaatg tgaggaagac gttattccag attcaccgat
     5401 aacagccttc tcattttctg gcgttaaccg gctacgaaga aaggagaacc cccatgtccg
     5461 atacatagaa caaacacata ctaaattgga gcactctgtg tgtgcaaatg aaatgagaaa
     5521 agtttccaag tcttcaactc atccacaaca taatcctaat gaaaatgaaa ttctagtagc
     5581 tgacacttat gaccaaagtc aatctccaat ggccaaagca catggaacaa gcagctatac
     5641 ccctgataag tcatctttta atttagctac agttgttgct gaaacacttg gacttggtgt
     5701 tcaagaagaa tctgaaactc aaggtcccat gagccccctt ggtgatgagc tctaccactg
     5761 tctggaagga aatcacaaga aacagccttt tgagggatcc aagcttatga atcccagtat
     5821 gaagcagaaa caagaagaaa tcaaagagaa tataaagaat agttctgtcc caagaagaac
     5881 tctgaagatg attcagcctt ctgcatctgg atctcttgtt ggaagagaaa atgagctgtc
     5941 cgcaggcttg tccaaaagga aacatcggaa tgaccactta acatctacaa cttccagccc
     6001 tggggttatt gtcccagaat ctagtgaaaa taaaaatctt ggaggagtca cccaggagtc
     6061 atttgatctt atgattaaag aaaatccatc ctctcagtat tggaaggaag tggcagaaaa
     6121 acggagaaag gcgctgtaga ccggttagta atgagtttaa acgggggagg ctaactgaaa
     6181 cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata
     6241 aaacgcacgg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac
     6301 tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc
     6361 ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca
     6421 ggccctgcca tagcagatct gcgcagctgg ggctctaggg ggtatcccca cgcgccctgt
     6481 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     6541 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     6601 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     6661 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     6721 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     6781 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     6841 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
     6901 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     6961 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     7021 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     7081 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     7141 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     7201 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
     7261 tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     7321 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     7381 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     7441 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
     7501 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     7561 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     7621 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     7681 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     7741 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     7801 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
     7861 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     7921 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     7981 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     8041 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     8101 gagcgggact ctggggttcg cgaaatgacc gaccaagcga cgcccaacct gccatcacga
     8161 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     8221 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     8281 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     8341 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     8401 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     8461 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     8521 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     8581 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     8641 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     8701 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     8761 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     8821 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     8881 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     8941 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     9001 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     9061 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     9121 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     9181 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     9241 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     9301 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     9361 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     9421 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     9481 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     9541 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     9601 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     9661 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     9721 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     9781 gcaataaacc agccagccgg aaggaccgag cgcagaagtg gtcctgcaac tttatccgcc
     9841 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     9901 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     9961 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
    10021 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
    10081 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
    10141 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    10201 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    10261 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    10321 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    10381 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    10441 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
    10501 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    10561 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
    10621 ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
    10681 agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag
    10741 ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
//
