LOCUS       sequence-211157-       12250 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Cas9 fusion to human CtIP protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12250)
  AUTHORS   Charpentier M, Khedher AHY, Menoret S, Brion A, Lamribet K, 
            Dardillac E, Boix C, Perrouault L, Tesson L, Geny S, De Cian A, 
            Itier JM, Anegon I, Lopez B, Giovannangeli C, Concordet JP
  TITLE     CtIP fusion to Cas9 enhances transgene integration by 
            homology-dependent repair.
  JOURNAL   Nat Commun. 2018 Mar 19;9(1):1133. doi: 10.1038/s41467-018-03475-7.
  PUBMED    29556040
REFERENCE   2  (bases 1 to 12250)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-03-19"; volume: "9(1):1133. doi"; pages: " 
            10.1038/s41467-018-03475-7"
COMMENT     Sequence Label: pCas9-CtIP
FEATURES             Location/Qualifiers
     source          1..12250
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        44..423
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        424..627
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     578..598
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     624..648
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      736..745
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             742..4845
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             742..4845
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             742..4845
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4858..4878
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(7630..7649)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7674..7722
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7924..8352
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8011..8030)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8221..8242
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8361..8381)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8366..8695
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8546..8681
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8608..8627
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             8762..9556
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8816..8835)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9426..9445
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    9732..9853
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9769..9788)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9823..9842
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9902..9918)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9902..9918)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9915..9937)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9926..9942
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9950..9956,9957..9974,9975..9980))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9995..10016
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10133..10150)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10304..10892)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10384..10403)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11063..11854,11855..11923))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11686..11705
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11924..12028)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12103..12122)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact
       61 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
      121 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      181 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      241 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      301 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      361 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      421 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      481 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      541 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      601 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
      661 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccggactcta
      721 gaggatcgaa cccttgccac catggacaag aagtactcca ttgggctcga tatcggcaca
      781 aacagcgtcg gctgggccgt cattacggac gagtacaagg tgccgagcaa aaaattcaaa
      841 gttctgggca ataccgatcg ccacagcata aagaagaacc tcattggcgc cctcctgttc
      901 gactccgggg agacggccga agccacgcgg ctcaaaagaa cagcacggcg cagatatacc
      961 cgcagaaaga atcggatctg ctacctgcag gagatcttta gtaatgagat ggctaaggtg
     1021 gatgactctt tcttccatag gctggaggag tcctttttgg tggaggagga taaaaagcac
     1081 gagcgccacc caatctttgg caatatcgtg gacgaggtgg cgtaccatga aaagtaccca
     1141 accatatatc atctgaggaa gaagcttgta gacagtactg ataaggctga cttgcggttg
     1201 atctatctcg cgctggcgca tatgatcaaa tttcggggac acttcctcat cgagggggac
     1261 ctgaacccag acaacagcga tgtcgacaaa ctctttatcc aactggttca gacttacaat
     1321 cagcttttcg aagagaaccc gatcaacgca tccggagttg acgccaaagc aatcctgagc
     1381 gctaggctgt ccaaatcccg gcggctcgaa aacctcatcg cacagctccc tggggagaag
     1441 aagaacggcc tgtttggtaa tcttatcgcc ctgtcactcg ggctgacccc caactttaaa
     1501 tctaacttcg acctggccga agatgccaag cttcaactga gcaaagacac ctacgatgat
     1561 gatctcgaca atctgctggc ccagatcggc gaccagtacg cagacctttt tttggcggca
     1621 aagaacctgt cagacgccat tctgctgagt gatattctgc gagtgaacac ggagatcacc
     1681 aaagctccgc tgagcgctag tatgatcaag cgctatgatg agcaccacca agacttgact
     1741 ttgctgaagg cccttgtcag acagcaactg cctgagaagt acaaggaaat tttcttcgat
     1801 cagtctaaaa atggctacgc cggatacatt gacggcggag caagccagga ggaattttac
     1861 aaatttatta agcccatctt ggaaaaaatg gacggcaccg aggagctgct ggtaaagctt
     1921 aacagagaag atctgttgcg caaacagcgc actttcgaca atggaagcat cccccaccag
     1981 attcacctgg gcgaactgca cgctatcctc aggcggcaag aggatttcta cccctttttg
     2041 aaagataaca gggaaaagat tgagaaaatc ctcacatttc ggatacccta ctatgtaggc
     2101 cccctcgccc ggggaaattc cagattcgcg tggatgactc gcaaatcaga agagaccatc
     2161 actccctgga acttcgagga agtcgtggat aagggggcct ctgcccagtc cttcatcgaa
     2221 aggatgacta actttgataa aaatctgcct aacgaaaagg tgcttcctaa acactctctg
     2281 ctgtacgagt acttcacagt ttataacgag ctcaccaagg tcaaatacgt cacagaaggg
     2341 atgagaaagc cagcattcct gtctggagag cagaagaaag ctatcgtgga cctcctcttc
     2401 aagacgaacc ggaaagttac cgtgaaacag ctcaaagaag actatttcaa aaagattgaa
     2461 tgtttcgact ctgttgaaat cagcggagtg gaggatcgct tcaacgcatc cctgggaacg
     2521 tatcacgatc tcctgaaaat cattaaagac aaggacttcc tggacaatga ggagaacgag
     2581 gacattcttg aggacattgt cctcaccctt acgttgtttg aagataggga gatgattgaa
     2641 gaacgcttga aaacttacgc tcatctcttc gacgacaaag tcatgaaaca gctcaagagg
     2701 cgccgatata caggatgggg gcggctgtca agaaaactga tcaatgggat ccgagacaag
     2761 cagagtggaa agacaatcct ggattttctt aagtccgatg gatttgccaa ccggaacttc
     2821 atgcagttga tccatgatga ctctctcacc tttaaggagg acatccagaa agcacaagtt
     2881 tctggccagg gggacagtct tcacgagcac atcgctaatc ttgcaggtag cccagctatc
     2941 aaaaagggaa tactgcagac cgttaaggtc gtggatgaac tcgtcaaagt aatgggaagg
     3001 cataagcccg agaatatcgt tatcgagatg gcccgagaga accaaactac ccagaaggga
     3061 cagaagaaca gtagggaaag gatgaagagg attgaagagg gtataaaaga actggggtcc
     3121 caaatcctta aggaacaccc agttgaaaac acccagcttc agaatgagaa gctctacctg
     3181 tactacctgc agaacggcag ggacatgtac gtggatcagg aactggacat caatcggctc
     3241 tccgactacg acgtggatca tatcgtgccc cagtcttttc tcaaagatga ttctattgat
     3301 aataaagtgt tgacaagatc cgataaaaat agagggaaga gtgataacgt cccctcagaa
     3361 gaagttgtca agaaaatgaa aaattattgg cggcagctgc tgaacgccaa actgatcaca
     3421 caacggaagt tcgataatct gactaaggct gaacgaggtg gcctgtctga gttggataaa
     3481 gccggcttca tcaaaaggca gcttgttgag acacgccaga tcaccaagca cgtggcccaa
     3541 attctcgatt cacgcatgaa caccaagtac gatgaaaatg acaaactgat tcgagaggtg
     3601 aaagttatta ctctgaagtc taagctggtc tcagatttca gaaaggactt tcagttttat
     3661 aaggtgagag agatcaacaa ttaccaccat gcgcatgatg cctacctgaa tgcagtggta
     3721 ggcactgcac ttatcaaaaa atatcccaag cttgaatctg aatttgttta cggagactat
     3781 aaagtgtacg atgttaggaa aatgatcgca aagtctgagc aggaaatagg caaggccacc
     3841 gctaagtact tcttttacag caatattatg aattttttca agaccgagat tacactggcc
     3901 aatggagaga ttcggaagcg accacttatc gaaacaaacg gagaaacagg agaaatcgtg
     3961 tgggacaagg gtagggattt cgcgacagtc cggaaggtcc tgtccatgcc gcaggtgaac
     4021 atcgttaaaa agaccgaagt acagaccgga ggcttctcca aggaaagtat cctcccgaaa
     4081 aggaacagcg acaagctgat cgcacgcaaa aaagattggg accccaagaa atacggcgga
     4141 ttcgattctc ctacagtcgc ttacagtgta ctggttgtgg ccaaagtgga gaaagggaag
     4201 tctaaaaaac tcaaaagcgt caaggaactg ctgggcatca caatcatgga gcgatcaagc
     4261 ttcgaaaaaa accccatcga ctttctcgag gcgaaaggat ataaagaggt caaaaaagac
     4321 ctcatcatta agcttcccaa gtactctctc tttgagcttg aaaacggccg gaaacgaatg
     4381 ctcgctagtg cgggcgagct gcagaaaggt aacgagctgg cactgccctc taaatacgtt
     4441 aatttcttgt atctggccag ccactatgaa aagctcaaag ggtctcccga agataatgag
     4501 cagaagcagc tgttcgtgga acaacacaaa cactaccttg atgagatcat cgagcaaata
     4561 agcgaattct ccaaaagagt gatcctcgcc gacgctaacc tcgataaggt gctttctgct
     4621 tacaataagc acagggataa gcccatcagg gagcaggcag aaaacattat ccacttgttt
     4681 actctgacca acttgggcgc gcctgcagcc ttcaagtact tcgacaccac catagacaga
     4741 aagcggtaca cctctacaaa ggaggtcctg gacgccacac tgattcatca gtcaattacg
     4801 gggctctatg aaacaagaat cgacctctct cagctcggtg gagacagcag ggctgacccc
     4861 aagaagaaga ggaaggtggg atccatgaac atctcgggaa gcagctgtgg aagccctaac
     4921 tctgcagata catctagtga ctttaaagat ctgtggacca aactaaaaga atgtcatgat
     4981 agagaagtac aaggtttaca agtaaaagta accaagctta agcaagagcg aatcttagat
     5041 gcacaaagac tagaagaatt cttcaccaaa aatcaacagc tgagggaaca gcagaaagtc
     5101 cttcatgaag ccattaaagt tttagaagat cggttaagag caggcttatg tgatcgctgt
     5161 gcagtaactg aagaacatat gcggaaaaaa cagcaagagt ttgaaaatat ccggcagcag
     5221 aatcttaaac ttattacaga acttatgaat gaaaggaata ctctacagga agaaaataaa
     5281 aagctttctg aacaactcca gcagaaaatt gagaatgatc aacagcatca agcagctgag
     5341 cttgaatgtg aggaagacgt tattccagat tcaccgataa cagccttctc attttctggc
     5401 gttaaccggc tacgaagaaa ggagaacccc catgtccgat acatagaaca aacacatact
     5461 aaattggagc actctgtgtg tgcaaatgaa atgagaaaag tttccaagtc ttcaactcat
     5521 ccacaacata atcctaatga aaatgaaatt ctagtagctg acacttatga ccaaagtcaa
     5581 tctccaatgg ccaaagcaca tggaacaagc agctataccc ctgataagtc atcttttaat
     5641 ttagctacag ttgttgctga aacacttgga cttggtgttc aagaagaatc tgaaactcaa
     5701 ggtcccatga gcccccttgg tgatgagctc taccactgtc tggaaggaaa tcacaagaaa
     5761 cagccttttg aggaatctac aagaaatact gaagatagtt taagattttc agattctact
     5821 tcaaagactc ctcctcaaga agaattacct actcgagtgt catctcctgt atttggagct
     5881 acctctagta tcaaaagtgg tttagatttg aatacaagtt tgtccccttc tcttttacag
     5941 cctgggaaaa aaaaacatct gaaaacactc ccttttagca acacttgtat atctagatta
     6001 gaaaaaacta gatcaaaatc tgaagatagt gcccttttca cacatcacag tcttgggtct
     6061 gaagtgaaca agatcattat ccagtcatct aataaacaga tacttataaa taaaaatata
     6121 agtgaatccc taggtgaaca gaataggact gagtacggta aagattctaa cactgataaa
     6181 catttggagc ccctgaaatc attgggaggc cgaacatcca aaaggaagaa aactgaggaa
     6241 gaaagtgaac atgaagtaag ctgcccccaa gcttcttttg ataaagaaaa tgctttccct
     6301 tttccaatgg ataatcagtt ttccatgaat ggagactgtg tgatgtataa acctctggat
     6361 ctgtctgatc gattttcagc tattcagcgt caagagaaaa gccaaggaag tgagacttct
     6421 aaaaacaaat ttaggcaagt gactctttat gaggctttga agaccattcc aaagggcttt
     6481 tcctcaagcc gtaaggcctc agatggcaac tgcacgttgc ccaaagattc cccaggggag
     6541 ccctgttcac aggaatgcat catccttcag cccttgaata aatgctctcc agacaataaa
     6601 ccatcattac aaataaaaga agaaaatgct gtctttaaaa ttcctctacg tccacgtgaa
     6661 agtttggaga ctgagaatgt tttagatgac ataaagagtg ctggttctca tgagccaata
     6721 aaaatacaaa ccaggtcaga ccatggagga tgtgaacttg catcagttct tcagttaaat
     6781 ccatgtagaa ctggtaaaat aaagtctcta caaaacaacc aagatgtatc ctttgaaaat
     6841 atccagtgga gtatagatcc gggagcagac ctttctcagt ataaaatgga tgttactgta
     6901 atagatacaa aggatggcag tcagtcaaaa ttaggaggag agacagtgga catggactgt
     6961 acattggtta gtgaaaccgt tctcttaaaa atgaagaaac aagagcagaa gggagaaaaa
     7021 agttcaatgc tcttttacat agatgaagaa agaaaaatga atgatagctt ggaagatatg
     7081 tttgatcgga caacacatga agagtatgaa tcctgtttgg cagacagttt ctcccaagca
     7141 gcagatgaag aggaggaatt gtctactgcc acaaagaaac tacacactca tggtgataaa
     7201 caagacaaag tcaagcagaa agcgtttgtg gagccgtatt ttaaaggtga tgaaagagag
     7261 actagcttgc aaaattttcc tcatattgag gtggttcgga aaaaagagga gagaagaaaa
     7321 ctgcttgggc acacgtgtaa ggaatgtgaa atttattatg cagatatgcc agcagaagaa
     7381 agagaaaaga aattggcttc ctgctcaaga caccgattcc gctacattcc acccaacaca
     7441 ccagagaatt tttgggaagt tggttttcct tccactcaga cttgtatgga aagaggttat
     7501 attaaggaag atcttgatcc ttgtcctcgt ccaaaaagac gtcagcctta caacgcaata
     7561 ttttctccaa aaggcaagga gcagaagaca tagaccggtt agtaatgagt ttaaacgggg
     7621 gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat
     7681 aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt tcataaacgc ggggttcggt
     7741 cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg
     7801 tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca
     7861 acgtcggggc ggcaggccct gccatagcag atctgcgcag ctggggctct agggggtatc
     7921 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     7981 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     8041 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     8101 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     8161 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     8221 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     8281 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     8341 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     8401 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     8461 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     8521 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     8581 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     8641 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     8701 cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg
     8761 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     8821 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     8881 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     8941 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     9001 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     9061 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     9121 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     9181 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     9241 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     9301 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     9361 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     9421 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     9481 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     9541 tgacgagttc ttctgagcgg gactctgggg ttcgcgaaat gaccgaccaa gcgacgccca
     9601 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     9661 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     9721 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     9781 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     9841 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     9901 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     9961 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
    10021 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
    10081 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
    10141 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
    10201 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
    10261 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
    10321 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
    10381 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
    10441 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
    10501 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
    10561 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
    10621 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
    10681 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
    10741 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
    10801 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
    10861 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
    10921 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
    10981 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
    11041 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
    11101 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
    11161 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
    11221 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
    11281 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
    11341 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
    11401 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    11461 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    11521 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    11581 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    11641 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    11701 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    11761 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    11821 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    11881 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    11941 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    12001 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
    12061 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
    12121 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
    12181 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
    12241 tagggttagg
//
