LOCUS       sequence-211159-        8754 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of mTagBFP2-tagged Lifeact with Puromycin 
            selection in cells including neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8754)
  TITLE     Lifeact Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti Lifeact-mTagBFP2 PuroR
FEATURES             Location/Qualifiers
     source          1..8754
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             54..234
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    306..427
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(343..362)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     397..416
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      467..602
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(622..641)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(623..641)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(651..668)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(651..667)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(660..682)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      809..1264
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(896..915)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1106..1127
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1290..1394
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1395..1463,1464..2255)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1613..1632)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2426..3014
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2915..2934
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3168..3185
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3302..3323
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3338..3343,3344..3361,3362..3368)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3376..3392
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3381..3403
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3400..3416
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3400..3416
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3435..3455
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3437..3455
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3483..3709
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3710..3890
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3937..4062
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4555..4788
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4973..5017
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5284..5400
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    5457..5481
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5597..5800
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5751..5771
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5797..5821
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      5877..5886
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5883..5933
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     regulatory      5949..5958
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5955..6665
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     protein_bind    6738..6758
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    complement(6870..6894)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6925..7513
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6978..6998)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7396..7407)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7527..8026
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7562..7585)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7959..7978
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8061..8660
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8061..8080)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8557..8577
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 tggaagggct agctcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
       61 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      121 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      181 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
      241 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
      301 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
      361 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
      421 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
      481 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
      541 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
      601 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
      661 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
      721 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
      781 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
      841 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
      901 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
      961 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     1021 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     1081 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     1141 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     1201 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     1261 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     1321 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     1381 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     1441 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     1501 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     1561 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     1621 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     1681 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     1741 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     1801 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     1861 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     1921 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     1981 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     2041 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     2101 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     2161 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     2221 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     2281 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     2341 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     2401 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     2461 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     2521 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     2581 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     2641 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     2701 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     2761 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     2821 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     2881 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     2941 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     3001 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     3061 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     3121 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     3181 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     3241 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     3301 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     3361 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     3421 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
     3481 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
     3541 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
     3601 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
     3661 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
     3721 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     3781 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     3841 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     3901 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     3961 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     4021 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     4081 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     4141 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     4201 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     4261 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     4321 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     4381 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
     4441 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     4501 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     4561 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     4621 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     4681 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     4741 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     4801 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     4861 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     4921 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     4981 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     5041 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     5101 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     5161 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     5221 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     5281 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     5341 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     5401 atcgtccagt gtggtggaat tgagtattcc agtgtggtgg aattctgcag atatcaacaa
     5461 gtttgtacaa aaaagcaggc tggagaccca agcttggtac cgagctcgga tccactagta
     5521 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     5581 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
     5641 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
     5701 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
     5761 cggtaggcgt gtacggtggg aggtttatat aagcagagct cgtttagtga accgtcagat
     5821 cgcctggaga cgccatccac gctgttttga cctccataga agattctaga gctagcgcca
     5881 ccatgggcgt ggccgacttg atcaagaagt tcgagtccat ctccaaggag gagggggatc
     5941 caccggtcgc caccatggtg tctaagggcg aagagctgat taaggagaac atgcacatga
     6001 agctgtacat ggagggcacc gtggacaacc atcacttcaa gtgcacatcc gagggcgaag
     6061 gcaagcccta cgagggcacc cagaccatga gaatcaaggt ggtcgagggc ggccctctcc
     6121 ccttcgcctt cgacatcctg gctactagct tcctctacgg cagcaagacc ttcatcaacc
     6181 acacccaggg catccccgac ttcttcaagc agtccttccc tgagggcttc acatgggaga
     6241 gagtcaccac atacgaagac gggggcgtgc tgaccgctac ccaggacacc agcctccagg
     6301 acggctgcct catctacaac gtcaagatca gaggggtgaa cttcacatcc aacggccctg
     6361 tgatgcagaa gaaaacactc ggctgggagg ccttcaccga gacgctgtac cccgctgacg
     6421 gcggcctgga aggcagaaac gacatggccc tgaagctcgt gggcgggagc catctgatcg
     6481 caaacgccaa gaccacatat agatccaaga aacccgctaa gaacctcaag atgcctggcg
     6541 tctactatgt ggactacaga ctggaaagaa tcaaggaggc caacaacgag acctacgtcg
     6601 agcagcacga ggtggcagtg gccagatact gcgacctccc tagcaaactg gggcacaagc
     6661 ttaattaagc ggccgcgact ctagaattct gcagatatcc atcacactgg cggccgctcg
     6721 agcatgcatc tagaggacaa ctttgtatac aaaagttggg agacccaagc ttggtaccga
     6781 gctcggatcc actagtaacg gccgccagtg tgctggaatt ctgcagatat ccatcacact
     6841 ggcggccgct cgagcatgca tctagaggta cccagctttc ttgtacaaag tggttgatat
     6901 ccagcacagt ggcggccgct cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     6961 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     7021 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     7081 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     7141 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     7201 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     7261 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag
     7321 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     7381 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     7441 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     7501 gccgcctccc cgcctggaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg
     7561 agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg
     7621 cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc
     7681 gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg
     7741 caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc
     7801 tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc
     7861 tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag
     7921 gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg
     7981 cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct gcagcccaag
     8041 cttaccacac tcctgcaggc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
     8101 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     8161 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     8221 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
     8281 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     8341 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     8401 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
     8461 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     8521 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     8581 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
     8641 cccgcaagcc cggtgcctga acgcgtatgc atctcgaggg cccggtacct ttaagaccaa
     8701 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggac
//
