LOCUS       sequence-211162-        7398 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length BMR1_02g04260 
            ectodomain with no N-linked glycans upon cotransfection with BirA in
            mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7398)
  AUTHORS   Elton CM, Rodriguez M, Ben Mamoun C, Lobo CA, Wright GJ
  TITLE     A library of recombinant Babesia microti cell surface and secreted 
            proteins for diagnostics discovery and reverse vaccinology.
  JOURNAL   Int J Parasitol. 2019 Feb;49(2):115-125. doi: 
            10.1016/j.ijpara.2018.10.003. Epub 2018 Oct 24.
  PUBMED    30367868
REFERENCE   2  (bases 1 to 7398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ijpara.2018.10.003"; journalName: "Int J Parasitol"; 
            date: "2019-02"; volume: "49"; issue: "2"; pages: "115-125"
COMMENT     Sequence Label: BMR1_02g04260-bio-His
FEATURES             Location/Qualifiers
     source          1..7398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2562..2579
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2631..2686
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2685..2704)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4922..4940)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5008..5112
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5113..5181,5182..5973)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5331..5350)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6144..6732
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6633..6652
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6886..6903
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7020..7041
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7056..7061,7062..7079,7080..7086)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7094..7110
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7099..7121
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7118..7134
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7118..7134
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccg
     1201 gcgatacaga tggcggacac tctcagggcc tggtgcagct ggcccagaaa ctggaccggt
     1261 tcatgatctt cgtgaagatc aacctggccg acacctacaa tctggccgtg gacaagctga
     1321 agctggacat ggaacacaag tgccacaaag agtacatcaa agagaaggac atcgaagagg
     1381 acaagatgaa gaacaagctc gagagcgcca tcaacgagat cgagatctgc gagaagaaag
     1441 agttcatcgg catcctggac aacgtggcca ccgcctacgc caactacaag agcctggtgg
     1501 acgaggtgta cagcaaggcc tacggcaccc tgggcggcga cgtggccaag gacttcgagt
     1561 acgagtgcaa gaagcggaag gccaacgccc tggccaacct gctggacgtg tgggacagct
     1621 tcgtgggcgt gctgaccaac gccatcggcg aggacaaagt gaaggacagc aagaaggcct
     1681 tcgacgagca cgtgtccaag gccaccgacc tgcgggacaa cgtgtacaac aaggacgagg
     1741 actacaacac ccacttcaag gacatgatca aagaagtgaa cggcgccacc gaggaactga
     1801 acaaagtgat cgacgtgatg atcagcaaca tcggcgacta cggcatcgtg atcgacagcg
     1861 gcatcatcag cagcatcaag agcctgatca gcaccatgac caagatctct ggcgcgccgt
     1921 cgacctccat cacggcctat aagagtgagg gggagtcagc ggagttctcc ttcccactca
     1981 accttggaga ggaaagcctg cagggagagt tgagatggaa ggcagagaag gctccttctt
     2041 cccagtcctg gatcaccttc tccctaaaga accaaaaggt gtctgtgcag aagtctacta
     2101 gcaaccccaa gttccagctg tccgaaacgc tcccactcac ccttcagata ccccaggtct
     2161 cccttcagtt tgctggttct ggcaacctga ccctgactct ggacagaggg atactgtatc
     2221 aggaagtgaa cctggtggtg atgaaagtga ctcagcccga cagcaacact ttgacctgtg
     2281 aggtgatggg acccacctca cccaagatga gactgatctt gaagcaggag aatcaggagg
     2341 ccagggtctc caggcaggag aaagtgattc aagtgcaggc ccctgaagca ggggtgtggc
     2401 aatgtctact gagtgaaggt gaagaggtca agatggactc caagatccag gttttatcca
     2461 aagggttgaa ttccggcagc ctgcaccaca tcctggacgc ccagaagatg ctgtggaacc
     2521 acagggacag gaacctgcct cctctggccc ctctgggccc ccaccaccat caccaccatt
     2581 gagtctagac acgtgattaa ttaaggatcc cccgacctcg acctctggct aataaaggaa
     2641 atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata
     2701 tgggagggca aatcatttgg tcgagatccc tcggagatct ctagctagag gatcgatccc
     2761 cgccccggac gaactaaacc tgactacgac atctctgccc cttcttcgcg gggcagtgca
     2821 tgtaatccct tcagttggtt ggtacaactt gccaactggg ccctgttcca catgtgacac
     2881 ggggggggac caaacacaaa ggggttctct gactgtagtt gacatcctta taaatggatg
     2941 tgcacatttg ccaacactga gtggctttca tcctggagca gactttgcag tctgtggact
     3001 gcaacacaac attgccttta tgtgtaactc ttggctgaag ctcttacacc aatgctgggg
     3061 gacatgtacc tcccaggggc ccaggaagac tacgggaggc tacaccaacg tcaatcagag
     3121 gggcctgtgt agctaccgat aagcggaccc tcaagagggc attagcaata gtgtttataa
     3181 ggcccccttg ttaaccctaa acgggtagca tatgcttccc gggtagtagt atatactatc
     3241 cagactaacc ctaattcaat agcatatgtt acccaacggg aagcatatgc tatcgaatta
     3301 gggttagtaa aagggtccta aggaacagcg atatctccca ccccatgagc tgtcacggtt
     3361 ttatttacat ggggtcagga ttccacgagg gtagtgaacc attttagtca caagggcagt
     3421 ggctgaagat caaggagcgg gcagtgaact ctcctgaatc ttcgcctgct tcttcattct
     3481 ccttcgttta gctaatagaa taactgctga gttgtgaaca gtaaggtgta tgtgaggtgc
     3541 tcgaaaacaa ggtttcaggt gacgccccca gaataaaatt tggacggggg gttcagtggt
     3601 ggcattgtgc tatgacacca atataaccct cacaaacccc ttgggcaata aatactagtg
     3661 taggaatgaa acattctgaa tatctttaac aatagaaatc catggggtgg ggacaagccg
     3721 taaagactgg atgtccatct cacacgaatt tatggctatg ggcaacacat aatcctagtg
     3781 caatatgata ctggggttat taagatgtgt cccaggcagg gaccaagaca ggtgaaccat
     3841 gttgttacac tctatttgta acaaggggaa agagagtgga cgccgacagc agcggactcc
     3901 actggttgtc tctaacaccc ccgaaaatta aacggggctc cacgccaatg gggcccataa
     3961 acaaagacaa gtggccactc ttttttttga aattgtggag tgggggcacg cgtcagcccc
     4021 cacacgccgc cctgcggttt tggactgtaa aataagggtg taataacttg gctgattgta
     4081 accccgctaa ccactgcggt caaaccactt gcccacaaaa ccactaatgg caccccgggg
     4141 aatacctgca taagtaggtg ggcgggccaa gataggggcg cgattgctgc gatctggagg
     4201 acaaattaca cacacttgcg cctgagcgcc aagcacaggg ttgttggtcc tcatattcac
     4261 gaggtcgctg agagcacggt gggctaatgt tgccatgggt agcatatact acccaaatat
     4321 ctggatagca tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat
     4381 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat
     4441 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     4501 ctgggtagta tatgctatcc taatctgtat ccgggtagca tatgctatcc taatagagat
     4561 tagggtagta tatgctatcc taatttatat ctgggtagca tatactaccc aaatatctgg
     4621 atagcatatg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     4681 gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     4741 gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat ctatatctgg
     4801 gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat ctgtatccgg
     4861 gtagcatatg ctatcctcat gataagctgt caaacatgag aattttcttg aagacgaaag
     4921 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     4981 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     5041 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     5101 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     5161 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     5221 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     5281 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     5341 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     5401 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     5461 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     5521 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     5581 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     5641 gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     5701 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     5761 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     5821 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     5881 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     5941 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     6001 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     6061 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     6121 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     6181 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     6241 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     6301 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     6361 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     6421 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     6481 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     6541 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     6601 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     6661 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     6721 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     6781 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     6841 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     6901 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     6961 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     7021 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     7081 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     7141 tacgccaagc tctagctaga ggtcgaccaa ttctcatgtt tgacagctta tcatcgcaga
     7201 tccgggcaac gttgttgcca ttgctgcagg cgcagaactg gtaggtatgg aagatctata
     7261 cattgaatca atattggcaa ttagccatat tagtcattgg ttatatagca taaatcaata
     7321 ttggctattg gccattgcat acgttgtatc tatatcataa tatgtacatt tatattggct
     7381 catgtccaat atgaccgc
//
