LOCUS       sequence-211163-       12438 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length BMR1_01g00210 
            ectodomain with no N-linked glycans upon cotransfection with BirA in
            mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12438)
  AUTHORS   Elton CM, Rodriguez M, Ben Mamoun C, Lobo CA, Wright GJ
  TITLE     A library of recombinant Babesia microti cell surface and secreted 
            proteins for diagnostics discovery and reverse vaccinology.
  JOURNAL   Int J Parasitol. 2019 Feb;49(2):115-125. doi: 
            10.1016/j.ijpara.2018.10.003. Epub 2018 Oct 24.
  PUBMED    30367868
REFERENCE   2  (bases 1 to 12438)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ijpara.2018.10.003"; journalName: "Int J Parasitol"; 
            date: "2019-02"; volume: "49"; issue: "2"; pages: "115-125"
COMMENT     Sequence Label: BMR1_01g00210-bio-His
FEATURES             Location/Qualifiers
     source          1..12438
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7602..7619
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    7671..7726
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7725..7744)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(9962..9980)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10048..10152
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10153..10221,10222..11013)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10371..10390)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11184..11772
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11673..11692
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11926..11943
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12060..12081
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12096..12101,12102..12119,12120..12126)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12134..12150
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12139..12161
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12158..12174
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12158..12174
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcct
     1201 ccgacggcaa gaagaagctg agcaacgccg acagcctgga actggacagc agcgagaaca
     1261 tcatcagcgt gctggaaagc agcatcgacg aggaaaacag catcaagtac ctgaacccca
     1321 acgagctgtc caacatcgac caggtcaaac tgttcgagca gaaagagctg ctgcggaccc
     1381 acatccccaa caagaacgcc aagaccgacc tgctggaacc cttcgtggac gtggactaca
     1441 agctgaacta cgaggaactg gaattcagcg gcctgaacat ccggatcccc ggcatgtgca
     1501 acgtgaacag cgacatcggc acattcaggc tgcaggaatg ggccgacagc aagtgcagcg
     1561 tgaacggcgc caccttctgc agcagatgcg agatggccag caccatcgtg tgcaagtgct
     1621 tccggatctg gcagatcaag ttcagctaca aagagaagtc ctgcctgacc aacgacggca
     1681 agctgacctt ctgctacggc accgtggact tcagcagcag cgacatcatc ctgaccaata
     1741 tcgcccctgc catcctgaag ttcagctcca tctacgagtt cgacagcgag agcagccagc
     1801 tgcacgaggg cctggaaggc ctgtgccacg gcaccaacga actcgtgcgg gtggaccaga
     1861 actacgtggc caagtgccac aaagagaaag aaatcgactt cttcatggaa gggaagtgct
     1921 tcggcgagtg caaagaaatc gtgccctgcc tgggcctgcc catcgacccc ctgaagtaca
     1981 gcagcctgct gatcgtgaac gactggaaca gcatcagcaa gaactgccac atctacgaca
     2041 tcaactactt cggctacttt ggctgcagca cccaggacct gaaccgggcc agcaatgtga
     2101 acatcaacaa ggcctacagc gccaccgtga acaacgccgt gcagatcggc ggcagcatct
     2161 ccaagagctg catcaccctg gaaaacaaga tcctgtacgt gcagcccggc atccccttcg
     2221 acctgaacat tgcccctgcc cccagcgccc tgtacagctt caagatcgag ttccacaacc
     2281 acaactgcgg cagcaacgcc atcgaggaca tcgtgcggcc cttcccacgg aaggacaagc
     2341 ccctgatcaa gaacggcaga gtgatcggaa ttatcctgac cggcttcctg gcccccctgt
     2401 actccaacaa ggccatcaac atctgcggct gcatcagcag caagttctac aaccccaagg
     2461 acagcatccg gggcgactac atgtgcagca agaccaacca ctacatcatc tccctgggca
     2521 agatcaagat ccagaccccc tacatcgaca tcaacaattt cgccaacgtg ctggtggaca
     2581 agcccttcct gtgggacaac agctttctgg ccctggacgg catcatcatt gccaagagct
     2641 tctacaaggg cttcgacttc ctgaacgaga agtccctgat cgagaacttc tacaacgact
     2701 tcatctacgc cccctacagc aacagcaaca agggcaacct gcagaagtcc atcagcttca
     2761 tcagcgagat cagcaaggcc gacaccaagg tgctgatcaa gtttaccaac cccggcgagt
     2821 acatcctgtt cttcgtgtcc gataccaaga tcctgatcat cggcctgtac agcatcctgg
     2881 gctttttcat cggcaagggc gtgacctact ccctggaagt gggcgacatc agcagaggcc
     2941 acagcatctt ctacaagagc tggggcatcc ccagcgacca gaaccggttc atcatctccg
     3001 agtacagcta caaggactgc agcagacccc tgttcgtgtc ctccgacatc gtgaagtccg
     3061 gcaccttccc caccacctgg atcgccagca acttcaacaa gtacaccagc ggccagaatt
     3121 acgactccat cagcaccaag agcatcaacg aggacctgta caagcctttc cagttctgcg
     3181 cccagagcaa ggacggcacc atcctgaacc tgggcaccgg caacttccgg cagtacatcg
     3241 accacgagct ggacgacatc ttccacgtgc ccctgaacaa gtacatcaac gacgtgggca
     3301 acgacaacac cctgctgacc ctgcccgacc ggaacatggt catcaacggc cacctgtaca
     3361 accggaacat ctacgaggaa tacctgaaga actacgccat cgacctggac atcaacctgg
     3421 aacagagccc caacaacctg gccttcaaga acaacggcga catcttcaac cacatcgact
     3481 gcccctactc catctccacc ttcacctaca gaagctggga tctgctgacc cagttttggt
     3541 tcgacatccc ccggaaccct tacagcagct tcccctacct gctgagcttc tggtgctacc
     3601 ccaacctgaa caccgtgttc gtgttcacca gcaacagcga gaacctggcc cctatgtatc
     3661 tgaagaagat tgagttcgcc agacccaccc acttccagct gttcgtgaac tctgccggcg
     3721 tgttcgccct ggtgctgacc agcggcgaca acgccatcag cctgtataag ctggacccca
     3781 agattaagtt catcaagaag gccaattgcg ccaagagcct gaagtccccc agcggcttcg
     3841 ccatgatcag cctggacacc tacaacaaga tctacctgat cctggacatg cggagcaact
     3901 gcatcatcct gctggatggc aagctggacg agatcgacgt gctgtgcaac gacaagttcc
     3961 ccgagatcca gcagcccagc agcgtgtcct gcgtgcagcg cgagggcgtg atcatggaat
     4021 gcttcatcat ccagaccttc agcaacagcc tgctgcgggt gcaggtcaac aaagaaacaa
     4081 agaagatgtc tatcgccgag tacttcaacg gcggcgacaa tcagaatctg gcccctctgc
     4141 tgagataccc cctgagcgtg atcgccttct cctacaactc catcgtgttc atctatctga
     4201 tcgaggccgg caactacaga ctgaccatcc tgtccctgca caaggacaag atcaccttct
     4261 acggaaagct gaagctgaac atgctgcccc tgagccactt cgtgctgctg agcagcgccg
     4321 tgtggcccaa caactaccgg aacgactacc tgatcgtgct cagatacgag gtgtacgaag
     4381 agaacaacta catcccccac atcaacatcg tcagcatcca ggacaccatg agcctgccca
     4441 atatcaagta ccccagctcc gagagcctgc tgctgggcga gaagctgaca ctgcacccca
     4501 agttcgagag cgacattatc aagaacagcg acttcttcgc cagaagcagc cagatcacct
     4561 acaagatccg gctgtaccag accggcaccc tgaagtctgg caccaatgag ctgatcacca
     4621 agatcagcga cgaggacttc tccgagatcg cccacatcga caagttcagc ggcaaggtgt
     4681 acctggatat caccttcatc aagtatcccg tgctgagctt tcaggtcaca tacagcggcg
     4741 ccatccacga gaagaaaacc ctgtttgtgg tcaagaccct gtgccccgac ggccactact
     4801 ttggcaacgg cggctgcagc ctgtgcccca tcggcaccta taacaaggcc gatatgagcc
     4861 actggaaccg gtgcatccct tgccccgaga acgaggccac catcagcagc ggcagcttca
     4921 gcatcaacca gtgtctgtgc aagcccggct accacctgga aggcgacgac tacatccagg
     4981 tggaaggccg gaacgaccgg ggctgtgtgc cttgtcctgc cggcacctgg aaagaaattg
     5041 tggccaccaa gggctgcttc ggcagcggct gctaccctaa cagcgccagc accgtgaccg
     5101 gcagccccac cgacgaggac cggaagtgcg agtgcttccc cggctactac taccacaagg
     5161 gcgctggcga cgtgcgcgag tgcaagatgg ccgagaacgg ctactacagc aatggcggct
     5221 acctggccaa gcggctggaa tgccccacca acaccgccag cttcggaatc gcccgcagcg
     5281 tgaaggactg cgtgtgcaga cctggctacc acgccatcta caacaaggat ggcaccaatc
     5341 tggcctacat caagctgaaa acctccatcc tggaacggca ccccgagctg gtcaacgtgc
     5401 agaacgagat caacgtgtgc atcccctgcg gcctcaacaa gtataagagc gccatcggcc
     5461 tgagcggctg cctggactgc cccgccaata gcttcagcga cagcgacaat gccgtggcca
     5521 aatacgagtg caacctgtgc aaaccaggct actacgagag cggcaacccc gacgacccct
     5581 gcagagagtg ccctgagaac agcttctgcg tgggcagcag gcccctggac accagcctgc
     5641 agatcaacag cggccagttc gtgacctgcc ctgccaactc cgccaccatg cccccctact
     5701 acctgaatac cctgcccatc cactgcgtgt gtaacgaggg ctacgagccc accggccagc
     5761 tgagccagag cggcatgatc gagtgcgtgc ccgtgaagca gggccacttc aagttctcca
     5821 gcggcaacac caagggcctg ccttgccccc acggcagcat cacccagaat gtgggcgcca
     5881 ccagccagtt tatgtgcgtg tgcccccctg gcttctacat gatggacggc aagtgcaccc
     5941 cctgcctgat gggccacttc tgcctgggcg gcagagacat cgacggcaac ctgatccccc
     6001 cacagaagtg tatcgacgag aattccgcca ccctgaacac caacgccgcc aacgaactgg
     6061 attgcgtgtg caagcctgga tacacccgga tcaaccccgc cgacaagttc tgcaagcagt
     6121 gcccccccaa tcactacaag agcaccacca gcaacgaggc ctgcacccct tgcaggggcc
     6181 acagctccac aggcgatctg agaggcgcca cctctaaaga gaagtgcttc tgcgcccctg
     6241 gctactattt cgacaagatc tgcctgccct gcggcaagaa cgacatctac tgccctggcg
     6301 gaaagctgga aatcgacggc ctggccaaga cccacccccc cgtgaagtgc cccgacaata
     6361 ccctgatcac ccctggggtg gacgtggcca gcagctacga cgactgcaag tgcgccaagg
     6421 gctacgcctt caagagcctg gatgtgcaga acaaggccaa gatctgcgag aagtgccctc
     6481 ccggcaccta caagtccagc gtgtccgaca gcggctgcac cggcctgtgc cctctgaata
     6541 gcgccagctt cctgggcagc cagaactcca agcagtgcta ctgcctggaa aacttctacc
     6601 acgtgtccgg cggcacctgt ctgccctgcc ctgagggcag catctgcaaa ggcggcctgg
     6661 tgccctacgc catggaaaag ggctacgact ccgacttcga gatcaccagc gaccaccaca
     6721 ccaagccaat cgccatggaa ggctactacc tggacaagct gaagaacacc ctggacgtgt
     6781 ccgacgactg gaagtttatc aagtgcccta tcgagggcag ctgcctgggc aaggacgagt
     6841 gcctggacag catggaacac tacctgtgcg ccgaatgcaa gttcggctac accaacaatt
     6901 tcaacaaggg cgagcggtgt accccctgcc cagccaccag cgtgaacatt ggcgcgccgt
     6961 cgacctccat cacggcctat aagagtgagg gggagtcagc ggagttctcc ttcccactca
     7021 accttggaga ggaaagcctg cagggagagt tgagatggaa ggcagagaag gctccttctt
     7081 cccagtcctg gatcaccttc tccctaaaga accaaaaggt gtctgtgcag aagtctacta
     7141 gcaaccccaa gttccagctg tccgaaacgc tcccactcac ccttcagata ccccaggtct
     7201 cccttcagtt tgctggttct ggcaacctga ccctgactct ggacagaggg atactgtatc
     7261 aggaagtgaa cctggtggtg atgaaagtga ctcagcccga cagcaacact ttgacctgtg
     7321 aggtgatggg acccacctca cccaagatga gactgatctt gaagcaggag aatcaggagg
     7381 ccagggtctc caggcaggag aaagtgattc aagtgcaggc ccctgaagca ggggtgtggc
     7441 aatgtctact gagtgaaggt gaagaggtca agatggactc caagatccag gttttatcca
     7501 aagggttgaa ttccggcagc ctgcaccaca tcctggacgc ccagaagatg ctgtggaacc
     7561 acagggacag gaacctgcct cctctggccc ctctgggccc ccaccaccat caccaccatt
     7621 gagtctagac acgtgattaa ttaaggatcc cccgacctcg acctctggct aataaaggaa
     7681 atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata
     7741 tgggagggca aatcatttgg tcgagatccc tcggagatct ctagctagag gatcgatccc
     7801 cgccccggac gaactaaacc tgactacgac atctctgccc cttcttcgcg gggcagtgca
     7861 tgtaatccct tcagttggtt ggtacaactt gccaactggg ccctgttcca catgtgacac
     7921 ggggggggac caaacacaaa ggggttctct gactgtagtt gacatcctta taaatggatg
     7981 tgcacatttg ccaacactga gtggctttca tcctggagca gactttgcag tctgtggact
     8041 gcaacacaac attgccttta tgtgtaactc ttggctgaag ctcttacacc aatgctgggg
     8101 gacatgtacc tcccaggggc ccaggaagac tacgggaggc tacaccaacg tcaatcagag
     8161 gggcctgtgt agctaccgat aagcggaccc tcaagagggc attagcaata gtgtttataa
     8221 ggcccccttg ttaaccctaa acgggtagca tatgcttccc gggtagtagt atatactatc
     8281 cagactaacc ctaattcaat agcatatgtt acccaacggg aagcatatgc tatcgaatta
     8341 gggttagtaa aagggtccta aggaacagcg atatctccca ccccatgagc tgtcacggtt
     8401 ttatttacat ggggtcagga ttccacgagg gtagtgaacc attttagtca caagggcagt
     8461 ggctgaagat caaggagcgg gcagtgaact ctcctgaatc ttcgcctgct tcttcattct
     8521 ccttcgttta gctaatagaa taactgctga gttgtgaaca gtaaggtgta tgtgaggtgc
     8581 tcgaaaacaa ggtttcaggt gacgccccca gaataaaatt tggacggggg gttcagtggt
     8641 ggcattgtgc tatgacacca atataaccct cacaaacccc ttgggcaata aatactagtg
     8701 taggaatgaa acattctgaa tatctttaac aatagaaatc catggggtgg ggacaagccg
     8761 taaagactgg atgtccatct cacacgaatt tatggctatg ggcaacacat aatcctagtg
     8821 caatatgata ctggggttat taagatgtgt cccaggcagg gaccaagaca ggtgaaccat
     8881 gttgttacac tctatttgta acaaggggaa agagagtgga cgccgacagc agcggactcc
     8941 actggttgtc tctaacaccc ccgaaaatta aacggggctc cacgccaatg gggcccataa
     9001 acaaagacaa gtggccactc ttttttttga aattgtggag tgggggcacg cgtcagcccc
     9061 cacacgccgc cctgcggttt tggactgtaa aataagggtg taataacttg gctgattgta
     9121 accccgctaa ccactgcggt caaaccactt gcccacaaaa ccactaatgg caccccgggg
     9181 aatacctgca taagtaggtg ggcgggccaa gataggggcg cgattgctgc gatctggagg
     9241 acaaattaca cacacttgcg cctgagcgcc aagcacaggg ttgttggtcc tcatattcac
     9301 gaggtcgctg agagcacggt gggctaatgt tgccatgggt agcatatact acccaaatat
     9361 ctggatagca tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat
     9421 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat
     9481 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     9541 ctgggtagta tatgctatcc taatctgtat ccgggtagca tatgctatcc taatagagat
     9601 tagggtagta tatgctatcc taatttatat ctgggtagca tatactaccc aaatatctgg
     9661 atagcatatg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     9721 gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     9781 gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat ctatatctgg
     9841 gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat ctgtatccgg
     9901 gtagcatatg ctatcctcat gataagctgt caaacatgag aattttcttg aagacgaaag
     9961 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
    10021 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
    10081 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
    10141 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
    10201 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
    10261 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
    10321 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
    10381 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
    10441 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
    10501 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
    10561 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
    10621 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
    10681 gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta
    10741 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
    10801 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
    10861 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
    10921 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
    10981 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
    11041 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
    11101 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
    11161 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
    11221 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
    11281 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
    11341 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
    11401 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
    11461 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
    11521 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
    11581 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
    11641 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
    11701 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
    11761 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
    11821 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
    11881 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
    11941 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
    12001 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
    12061 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
    12121 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
    12181 tacgccaagc tctagctaga ggtcgaccaa ttctcatgtt tgacagctta tcatcgcaga
    12241 tccgggcaac gttgttgcca ttgctgcagg cgcagaactg gtaggtatgg aagatctata
    12301 cattgaatca atattggcaa ttagccatat tagtcattgg ttatatagca taaatcaata
    12361 ttggctattg gccattgcat acgttgtatc tatatcataa tatgtacatt tatattggct
    12421 catgtccaat atgaccgc
//
