LOCUS       sequence-211165-        8583 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMV expression vector for BE3-2X construct (not codon optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8583)
  AUTHORS   Zafra MP, Schatoff EM, Katti A, Foronda M, Breinig M, Schweitzer AY,
            Simon A, Han T, Goswami S, Montgomery E, Thibado J, Kastenhuber ER, 
            Sanchez-Rivera FJ, Shi J, Vakoc CR, Lowe SW, Tschaharganeh DF, Dow 
            LE
  TITLE     Optimized base editors enable efficient editing in cells, organoids 
            and mice.
  JOURNAL   Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
  PUBMED    29969439
REFERENCE   2  (bases 1 to 8583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194."
COMMENT     Sequence Label: pCMV-BE3-2X
FEATURES             Location/Qualifiers
     source          1..8583
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        167..370
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     321..341
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     412..431
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        412..430
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             444..1130
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             1149..1169
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1179..1199
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1230..4628,4629..4631,4632..5228,5229,5230..5232,
                     5233..5234,5235..5237,5238..5330)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(1230..1253,1254..1256,1257..5330)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5343..5591
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5604..5624
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5633..5650
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5673..5690)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5679..5903
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5974..5990)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5974..5990)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5987..6009)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5998..6014
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6022..6028,6029..6046,6047..6052))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6067..6088
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6205..6222)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6376..6964)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6456..6475)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7135..7926,7927..7995))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7758..7777
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7996..8100)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8179..8198)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(8370..8583,1..166)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
       61 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      121 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      181 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      241 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      301 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      361 aagcagagct ggtttagtga accgtcagat ccgctagaga tccgcggccg ctaatacgac
      421 tcactatagg gagagccgcc accatgagct cagagactgg cccagtggct gtggacccca
      481 cattgagacg gcggatcgag ccccatgagt ttgaggtatt cttcgatccg agagagctcc
      541 gcaaggagac ctgcctgctt tacgaaatta attggggggg ccggcactcc atttggcgac
      601 atacatcaca gaacactaac aagcacgtcg aagtcaactt catcgagaag ttcacgacag
      661 aaagatattt ctgtccgaac acaaggtgca gcattacctg gtttctcagc tggagcccat
      721 gcggcgaatg tagtagggcc atcactgaat tcctgtcaag gtatccccac gtcactctgt
      781 ttatttacat cgcaaggctg taccaccacg ctgacccccg caatcgacaa ggcctgcggg
      841 atttgatctc ttcaggtgtg actatccaaa ttatgactga gcaggagtca ggatactgct
      901 ggagaaactt tgtgaattat agcccgagta atgaagccca ctggcctagg tatccccatc
      961 tgtgggtacg actgtacgtt cttgaactgt actgcatcat actgggcctg cctccttgtc
     1021 tcaacattct gagaaggaag cagccacagc tgacattctt taccatcgct cttcagtctt
     1081 gtcattacca gcgactgccc ccacacattc tctgggccac cgggttgaaa agcggcagcg
     1141 agactccccc aaagaagaaa cggaaagtag gcggctcccc caagaagaag cggaaggtag
     1201 ggacctcaga gtccgccaca cccgaaagtg ataaaaagta ttctattggt ttagccatcg
     1261 gcactaattc cgttggatgg gctgtcataa ccgatgaata caaagtacct tcaaagaaat
     1321 ttaaggtgtt ggggaacaca gaccgtcatt cgattaaaaa gaatcttatc ggtgccctcc
     1381 tattcgatag tggcgaaacg gcagaggcga ctcgcctgaa acgaaccgct cggagaaggt
     1441 atacacgtcg caagaaccga atatgttact tacaagaaat ttttagcaat gagatggcca
     1501 aagttgacga ttctttcttt caccgtttgg aagagtcctt ccttgtcgaa gaggacaaga
     1561 aacatgaacg gcaccccatc tttggaaaca tagtagatga ggtggcatat catgaaaagt
     1621 acccaacgat ttatcacctc agaaaaaagc tagttgactc aactgataaa gcggacctga
     1681 ggttaatcta cttggctctt gcccatatga taaagttccg tgggcacttt ctcattgagg
     1741 gtgatctaaa tccggacaac tcggatgtcg acaaactgtt catccagtta gtacaaacct
     1801 ataatcagtt gtttgaagag aaccctataa atgcaagtgg cgtggatgcg aaggctattc
     1861 ttagcgcccg cctctctaaa tcccgacggc tagaaaacct gatcgcacaa ttacccggag
     1921 agaagaaaaa tgggttgttc ggtaacctta tagcgctctc actaggcctg acaccaaatt
     1981 ttaagtcgaa cttcgactta gctgaagatg ccaaattgca gcttagtaag gacacgtacg
     2041 atgacgatct cgacaatcta ctggcacaaa ttggagatca gtatgcggac ttatttttgg
     2101 ctgccaaaaa ccttagcgat gcaatcctcc tatctgacat actgagagtt aatactgaga
     2161 ttaccaaggc gccgttatcc gcttcaatga tcaaaaggta cgatgaacat caccaagact
     2221 tgacacttct caaggcccta gtccgtcagc aactgcctga gaaatataag gaaatattct
     2281 ttgatcagtc gaaaaacggg tacgcaggtt atattgacgg cggagcgagt caagaggaat
     2341 tctacaagtt tatcaaaccc atattagaga agatggatgg gacggaagag ttgcttgtaa
     2401 aactcaatcg cgaagatcta ctgcgaaagc agcggacttt cgacaacggt agcattccac
     2461 atcaaatcca cttaggcgaa ttgcatgcta tacttagaag gcaggaggat ttttatccgt
     2521 tcctcaaaga caatcgtgaa aagattgaga aaatcctaac ctttcgcata ccttactatg
     2581 tgggacccct ggcccgaggg aactctcggt tcgcatggat gacaagaaag tccgaagaaa
     2641 cgattactcc atggaatttt gaggaagttg tcgataaagg tgcgtcagct caatcgttca
     2701 tcgagaggat gaccaacttt gacaagaatt taccgaacga aaaagtattg cctaagcaca
     2761 gtttacttta cgagtatttc acagtgtaca atgaactcac gaaagttaag tatgtcactg
     2821 agggcatgcg taaacccgcc tttctaagcg gagaacagaa gaaagcaata gtagatctgt
     2881 tattcaagac caaccgcaaa gtgacagtta agcaattgaa agaggactac tttaagaaaa
     2941 ttgaatgctt cgattctgtc gagatctccg gggtagaaga tcgatttaat gcgtcacttg
     3001 gtacgtatca tgacctccta aagataatta aagataagga cttcctggat aacgaagaga
     3061 atgaagatat cttagaagat atagtgttga ctcttaccct ctttgaagat cgggaaatga
     3121 ttgaggaaag actaaaaaca tacgctcacc tgttcgacga taaggttatg aaacagttaa
     3181 agaggcgtcg ctatacgggc tggggacgat tgtcgcggaa acttatcaac gggataagag
     3241 acaagcaaag tggtaaaact attctcgatt ttctaaagag cgacggcttc gccaatagga
     3301 actttatgca gctgatccat gatgactctt taaccttcaa agaggatata caaaaggcac
     3361 aggtttccgg acaaggggac tcattgcacg aacatattgc gaatcttgct ggttcgccag
     3421 ccatcaaaaa gggcatactc cagacagtca aagtagtgga tgagctagtt aaggtcatgg
     3481 gacgtcacaa accggaaaac attgtaatcg agatggcacg cgaaaatcaa acgactcaga
     3541 aggggcaaaa aaacagtcga gagcggatga agagaataga agagggtatt aaagaactgg
     3601 gcagccagat cttaaaggag catcctgtgg aaaataccca attgcagaac gagaaacttt
     3661 acctctatta cctacaaaat ggaagggaca tgtatgttga tcaggaactg gacataaacc
     3721 gtttatctga ttacgacgtc gatcacattg taccccaatc ctttttgaag gacgattcaa
     3781 tcgacaataa agtgcttaca cgctcggata agaaccgagg gaaaagtgac aatgttccaa
     3841 gcgaggaagt cgtaaagaaa atgaagaact attggcggca gctcctaaat gcgaaactga
     3901 taacgcaaag aaagttcgat aacttaacta aagctgagag gggtggcttg tctgaacttg
     3961 acaaggccgg atttattaaa cgtcagctcg tggaaacccg ccaaatcaca aagcatgttg
     4021 cacagatact agattcccga atgaatacga aatacgacga gaacgataag ctgattcggg
     4081 aagtcaaagt aatcacttta aagtcaaaat tggtgtcgga cttcagaaag gattttcaat
     4141 tctataaagt tagggagata aataactacc accatgcgca cgacgcttat cttaatgccg
     4201 tcgtagggac cgcactcatt aagaaatacc cgaagctaga aagtgagttt gtgtatggtg
     4261 attacaaagt ttatgacgtc cgtaagatga tcgcgaaaag cgaacaggag ataggcaagg
     4321 ctacagccaa atacttcttt tattctaaca ttatgaattt ctttaagacg gaaatcactc
     4381 tggcaaacgg agagatacgc aaacgacctt taattgaaac caatggggag acaggtgaaa
     4441 tcgtatggga taagggccgg gacttcgcga cggtgagaaa agttttgtcc atgccccaag
     4501 tcaacatagt aaagaaaact gaggtgcaga ccggagggtt ttcaaaggaa tcgattcttc
     4561 caaaaaggaa tagtgataag ctcatcgctc gtaaaaagga ctgggacccg aaaaagtacg
     4621 gtggcttcga tagccctaca gttgcctatt ctgtcctagt agtggcaaaa gttgagaagg
     4681 gaaaatccaa gaaactgaag tcagtcaaag aattattggg gataacgatt atggagcgct
     4741 cgtcttttga aaagaacccc atcgacttcc ttgaggcgaa aggttacaag gaagtaaaaa
     4801 aggatctcat aattaaacta ccaaagtata gtctgtttga gttagaaaat ggccgaaaac
     4861 ggatgttggc tagcgccgga gagcttcaaa aggggaacga actcgcacta ccgtctaaat
     4921 acgtgaattt cctgtattta gcgtcccatt acgagaagtt gaaaggttca cctgaagata
     4981 acgaacagaa gcaacttttt gttgagcagc acaaacatta tctcgacgaa atcatagagc
     5041 aaatttcgga attcagtaag agagtcatcc tagctgatgc caatctggac aaagtattaa
     5101 gcgcatacaa caagcacagg gataaaccca tacgtgagca ggcggaaaat attatccatt
     5161 tgtttactct taccaacctc ggcgctccag ccgcattcaa gtattttgac acaacgatag
     5221 atcgcaaacg atacacttct accaaggagg tgctagacgc gacactgatt caccaatcca
     5281 tcacgggatt atatgaaact cggatagatt tgtcacagct tgggggtgac tctggtggtt
     5341 ctactaatct gtcagatatt attgaaaagg agaccggtaa gcaactggtt atccaggaat
     5401 ccatcctcat gctcccagag gaggtggaag aagtcattgg gaacaagccg gaaagcgata
     5461 tactcgtgca caccgcctac gacgagagca ccgacgagaa tgtcatgctt ctgactagcg
     5521 acgcccctga atacaagcct tgggctctgg tcatacagga tagcaacggt gagaacaaga
     5581 ttaagatgct ctctggtggt tctcccaaga agaagaggaa agtctaaccg gtcatcatca
     5641 ccatcaccat tgagtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     5701 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     5761 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     5821 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     5881 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctcgatacc
     5941 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     6001 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctaggg
     6061 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     6121 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     6181 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     6241 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     6301 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     6361 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     6421 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     6481 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     6541 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     6601 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     6661 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     6721 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     6781 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     6841 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     6901 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     6961 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     7021 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     7081 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     7141 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     7201 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     7261 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     7321 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     7381 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     7441 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     7501 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     7561 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     7621 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     7681 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     7741 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     7801 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     7861 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     7921 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     7981 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     8041 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     8101 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcgat ctcccgatcc
     8161 cctagggtcg actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
     8221 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
     8281 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
     8341 tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta
     8401 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     8461 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     8521 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     8581 acg
//
