LOCUS       sequence-211168-        7494 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length BMR1_02g04181 
            ectodomain with no N-linked glycans upon cotransfection with BirA in
            mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7494)
  AUTHORS   Elton CM, Rodriguez M, Ben Mamoun C, Lobo CA, Wright GJ
  TITLE     A library of recombinant Babesia microti cell surface and secreted 
            proteins for diagnostics discovery and reverse vaccinology.
  JOURNAL   Int J Parasitol. 2019 Feb;49(2):115-125. doi: 
            10.1016/j.ijpara.2018.10.003. Epub 2018 Oct 24.
  PUBMED    30367868
REFERENCE   2  (bases 1 to 7494)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ijpara.2018.10.003"; journalName: "Int J Parasitol"; 
            date: "2019-02"; volume: "49"; issue: "2"; pages: "115-125"
COMMENT     Sequence Label: BMR1_02g04181-bio-His
FEATURES             Location/Qualifiers
     source          1..7494
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2658..2675
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2727..2782
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2781..2800)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5018..5036)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5104..5208
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5209..5277,5278..6069)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5427..5446)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6240..6828
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6729..6748
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6982..6999
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7116..7137
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7152..7157,7158..7175,7176..7182)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7190..7206
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7195..7217
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7214..7230
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7214..7230
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcca
     1201 caaaggccag ctacagagag ttccccgaga gcgccaagca gcagctggaa cggggcagag
     1261 ccgcctacgt ggaaatcatt gccaacgccg ccagcgccag caccatccct gagagcaccg
     1321 gcctggccaa tatcgccagc aaggacgtga acctgatcag cgtgaagcac atcagcgagg
     1381 gcgactgctg gaacttcgtg ctggaagagg tggaaagcct ggacctggaa aagtgcgagg
     1441 gggagggcga tagcagcaga agcctgatcg ccctggcccg gacccggtgc ttcttcttca
     1501 aatcccaacg gcccttccca accatcaagg acggctgcat cctgaacccc cggaacatcc
     1561 ccgccgagat gctgaaaacc cagttcccca acttcgagac aaacccctgc atcaacgact
     1621 acaagtccca cgactgcgag cggctgaagt tcgagatcgt gaagaagtgc accagcagca
     1681 agatcctgag cgacagcgcc ttccagatct tccacgccga tgtgaaccac atcgacgaca
     1741 tctgcttcta tctgcagtcc accgcctggc ggcagcggac cgagtacaac atcaacaagc
     1801 tgggcgagtc tgccggcacc atcatcgaga gccactccct gatccagagc tacctggaca
     1861 acatgaagat gaaccaggaa gaacagatcg tccaggccca gtggatgagc gagtggctga
     1921 gccaggtcaa aggcgagctg tccgacatgt tcaacatcct gatctctatc aagggctacc
     1981 actccaagat tctggacgcc gtgtctggcg cgccgtcgac ctccatcacg gcctataaga
     2041 gtgaggggga gtcagcggag ttctccttcc cactcaacct tggagaggaa agcctgcagg
     2101 gagagttgag atggaaggca gagaaggctc cttcttccca gtcctggatc accttctccc
     2161 taaagaacca aaaggtgtct gtgcagaagt ctactagcaa ccccaagttc cagctgtccg
     2221 aaacgctccc actcaccctt cagatacccc aggtctccct tcagtttgct ggttctggca
     2281 acctgaccct gactctggac agagggatac tgtatcagga agtgaacctg gtggtgatga
     2341 aagtgactca gcccgacagc aacactttga cctgtgaggt gatgggaccc acctcaccca
     2401 agatgagact gatcttgaag caggagaatc aggaggccag ggtctccagg caggagaaag
     2461 tgattcaagt gcaggcccct gaagcagggg tgtggcaatg tctactgagt gaaggtgaag
     2521 aggtcaagat ggactccaag atccaggttt tatccaaagg gttgaattcc ggcagcctgc
     2581 accacatcct ggacgcccag aagatgctgt ggaaccacag ggacaggaac ctgcctcctc
     2641 tggcccctct gggcccccac caccatcacc accattgagt ctagacacgt gattaattaa
     2701 ggatcccccg acctcgacct ctggctaata aaggaaattt attttcattg caatagtgtg
     2761 ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc atttggtcga
     2821 gatccctcgg agatctctag ctagaggatc gatccccgcc ccggacgaac taaacctgac
     2881 tacgacatct ctgccccttc ttcgcggggc agtgcatgta atcccttcag ttggttggta
     2941 caacttgcca actgggccct gttccacatg tgacacgggg ggggaccaaa cacaaagggg
     3001 ttctctgact gtagttgaca tccttataaa tggatgtgca catttgccaa cactgagtgg
     3061 ctttcatcct ggagcagact ttgcagtctg tggactgcaa cacaacattg cctttatgtg
     3121 taactcttgg ctgaagctct tacaccaatg ctgggggaca tgtacctccc aggggcccag
     3181 gaagactacg ggaggctaca ccaacgtcaa tcagaggggc ctgtgtagct accgataagc
     3241 ggaccctcaa gagggcatta gcaatagtgt ttataaggcc cccttgttaa ccctaaacgg
     3301 gtagcatatg cttcccgggt agtagtatat actatccaga ctaaccctaa ttcaatagca
     3361 tatgttaccc aacgggaagc atatgctatc gaattagggt tagtaaaagg gtcctaagga
     3421 acagcgatat ctcccacccc atgagctgtc acggttttat ttacatgggg tcaggattcc
     3481 acgagggtag tgaaccattt tagtcacaag ggcagtggct gaagatcaag gagcgggcag
     3541 tgaactctcc tgaatcttcg cctgcttctt cattctcctt cgtttagcta atagaataac
     3601 tgctgagttg tgaacagtaa ggtgtatgtg aggtgctcga aaacaaggtt tcaggtgacg
     3661 cccccagaat aaaatttgga cggggggttc agtggtggca ttgtgctatg acaccaatat
     3721 aaccctcaca aaccccttgg gcaataaata ctagtgtagg aatgaaacat tctgaatatc
     3781 tttaacaata gaaatccatg gggtggggac aagccgtaaa gactggatgt ccatctcaca
     3841 cgaatttatg gctatgggca acacataatc ctagtgcaat atgatactgg ggttattaag
     3901 atgtgtccca ggcagggacc aagacaggtg aaccatgttg ttacactcta tttgtaacaa
     3961 ggggaaagag agtggacgcc gacagcagcg gactccactg gttgtctcta acacccccga
     4021 aaattaaacg gggctccacg ccaatggggc ccataaacaa agacaagtgg ccactctttt
     4081 ttttgaaatt gtggagtggg ggcacgcgtc agcccccaca cgccgccctg cggttttgga
     4141 ctgtaaaata agggtgtaat aacttggctg attgtaaccc cgctaaccac tgcggtcaaa
     4201 ccacttgccc acaaaaccac taatggcacc ccggggaata cctgcataag taggtgggcg
     4261 ggccaagata ggggcgcgat tgctgcgatc tggaggacaa attacacaca cttgcgcctg
     4321 agcgccaagc acagggttgt tggtcctcat attcacgagg tcgctgagag cacggtgggc
     4381 taatgttgcc atgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat
     4441 ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     4501 ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat
     4561 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     4621 ctgtatccgg gtagcatatg ctatcctaat agagattagg gtagtatatg ctatcctaat
     4681 ttatatctgg gtagcatata ctacccaaat atctggatag catatgctat cctaatctat
     4741 atctgggtag catatgctat cctaatctat atctgggtag cataggctat cctaatctat
     4801 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatttat
     4861 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
     4921 atctgggtag tatatgctat cctaatctgt atccgggtag catatgctat cctcatgata
     4981 agctgtcaaa catgagaatt ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
     5041 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     5101 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     5161 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     5221 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     5281 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     5341 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     5401 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
     5461 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     5521 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     5581 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     5641 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     5701 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg
     5761 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     5821 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     5881 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     5941 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     6001 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     6061 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     6121 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     6181 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     6241 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     6301 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     6361 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     6421 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     6481 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     6541 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     6601 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     6661 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     6721 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     6781 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     6841 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     6901 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     6961 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     7021 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     7081 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     7141 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     7201 taacaatttc acacaggaaa cagctatgac catgattacg ccaagctcta gctagaggtc
     7261 gaccaattct catgtttgac agcttatcat cgcagatccg ggcaacgttg ttgccattgc
     7321 tgcaggcgca gaactggtag gtatggaaga tctatacatt gaatcaatat tggcaattag
     7381 ccatattagt cattggttat atagcataaa tcaatattgg ctattggcca ttgcatacgt
     7441 tgtatctata tcataatatg tacatttata ttggctcatg tccaatatga ccgc
//
