LOCUS       sequence-211169-        9603 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length BMR1_04g07915 
            ectodomain with no N-linked glycans upon cotransfection with BirA in
            mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9603)
  AUTHORS   Elton CM, Rodriguez M, Ben Mamoun C, Lobo CA, Wright GJ
  TITLE     A library of recombinant Babesia microti cell surface and secreted 
            proteins for diagnostics discovery and reverse vaccinology.
  JOURNAL   Int J Parasitol. 2019 Feb;49(2):115-125. doi: 
            10.1016/j.ijpara.2018.10.003. Epub 2018 Oct 24.
  PUBMED    30367868
REFERENCE   2  (bases 1 to 9603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ijpara.2018.10.003"; journalName: "Int J Parasitol"; 
            date: "2019-02"; volume: "49"; issue: "2"; pages: "115-125"
COMMENT     Sequence Label: BMR1_04g07915-bio-His
FEATURES             Location/Qualifiers
     source          1..9603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4767..4784
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4836..4891
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4890..4909)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7127..7145)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7213..7317
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7318..7386,7387..8178)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7536..7555)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8349..8937
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8838..8857
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9091..9108
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9225..9246
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9261..9266,9267..9284,9285..9291)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9299..9315
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9304..9326
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9323..9339
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9323..9339
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcca
     1201 gcatcgacag ccagaaaagc gacaacgacg tggccaacac cgccgagaca agcgagaagc
     1261 tgaactacta cgacgcccgg gacgacttcc tgcacaccat ggacatggtc aacctcgtgg
     1321 gcggcagctg caccctgatc gacaaggcca accagcccag cgacttcaag cagctgctga
     1381 acgccgccat cttcggctac ggccggatca gcgccctgct gacccagacc aagagcatct
     1441 acgacgtgac cgtggccagc accctggccg ccgtgttcgg caagctgatg cagatcaacc
     1501 tggacgacga gggcctggcc gtggaacagt ggtccgccag agtgtgcagc atgctggaaa
     1561 tcgccgaggc cagcctgctg agcaccagct tcaagatcct ggccgaccgg ctgaccaagc
     1621 actgcggcag gatcgccgag gtgttcctga gccagaaaga gaacgacccc atcgacaaga
     1681 acaaggccag cgacagcatc aacgactaca gcagcggcga gggcgtggac gtcgtgtggt
     1741 ccgtgtccac cgagtgcctg atggaattca ttgcccccaa cagccctggc ggcttcacca
     1801 gcaagtggac cttcgtgacc ttcaagcacc tcctgatgca gctgagcatc agcatcatca
     1861 tgtgcgagta ccagctgcgg gaccccaagc tgctggacga tgagatcgag ggggtggaaa
     1921 acctgctgtt ccggtcctgg gacgtgctgg aacactacag caacatccag ttctacaccc
     1981 agctggacgg cgccagcggc atcatgacct tcgcccagag caacctgatg cccctggtca
     2041 agcgggacgg cgacaagatc aacgtggcct ggaacctgca gaacgagttc gaggccgacc
     2101 ccagcagcaa ctctgccggc gatctgggac tggccgctgg cgataatgcc gccagcgacg
     2161 gagagaagcc cgtggccacc gaccccccat tctacagcag acggcccaga acctacagca
     2221 tgctgagcga ctacaagcgg cggctgagcc tgacctacga gatgagcgac gaggacaccc
     2281 ccgacgagga cgacgacttc gagagagagg acgaggaagt catcgtcgag aaagagacag
     2341 agagcacccc caacaagcag atcaagaacg ccaagaatgt gttcggcaga agaaagaccc
     2401 cccagaacaa ggtgttccgg cccaagctgt acccccacga caccgtgtct accagcagcg
     2461 tgacctacgc catcaagacc aacaacgaga accagatcca gttcaagagc ggagccgccc
     2521 agcccatcga cagcgacatc gatagcgtgc ccagagccaa ggctagcaac cccgccatcg
     2581 actccggcaa cgatgccgtg cctcgggcca aggcctccaa tcctgccatt gacagcggca
     2641 atgacgccgt gcccagggct aaagcctcta accccgccaa cggcgccgat atcgactccg
     2701 tgccacgcgc caaagccagc aatccagcca acggccctgg caacgacgct gtgccaagag
     2761 cccaggcttc caaccctgcc aacggacctg gaaatgatgc cgtcccaaga gccaaagcct
     2821 ccaagcccag caatggggcc gacatcgatt ctgtgcccac cgcccctgcc agcaagccct
     2881 ccaacggcgc tgacattgat agtgtgccta ccgctcccgc ctccaccagc aagagcgtgc
     2941 ggggaacaac cggcaccggc agcaacagca agtggtccag cgtgcggaac agcgtgctga
     3001 agaacaaagc cgaggaaaac gacaacaacg agggccccag caaggcctcc ggatctgccg
     3061 gcggaggact gaagggcgag atcagcttca gcgataccgt gtccatcctg cggttccgca
     3121 gcgagcccct gcggtggacc gagaacacca aggtggaagc caacatgcac cggaagatgg
     3181 tcaacaagtg ccaggtggaa cagctgcggc ccctgagcat cgactactac aacaagatca
     3241 gcccccccaa cctgggccac cccgtgatgg gcagagccac agccagcagc agctccaccc
     3301 tgctgcccaa gttcatgggc atcggcgcca aggtgtccaa gaccagcaac aatcccgcca
     3361 tggacaccct gagcaatttc tggggcatgc agcggcacga caaagagctg agcggcatcc
     3421 ggaagctgga caagaccgtg tcccagatgg ccatcctgga ccggttcagc cggctggccg
     3481 atacctgctg ggatgtggcc agcggcagac ccagcggcta catcagcccc gagacaatgt
     3541 accggctggg cggcaacaga aaggacgact acatccacca cgtgacccgg gtgtacccct
     3601 tcaacctggt caaggcctac cggttcctgg tcaccaaaga ggaaccccag gtccagagca
     3661 acggcgacga caatttcgag ttcagcctgt ttctgcaagc ccccgagttc gtgtctatcc
     3721 tgcccaacgg cgaccgggcc gaggccctgg tcattgagca gaaactggtg tccaccatcc
     3781 ggaccctgaa caccaacagc gccgaggacc cccccatcag cgtgtgcggc tacgtgtaca
     3841 agcggaacga cgtggaccgg gtgctgatcg gcgagttcgg cgtgaagctg cccagcacca
     3901 tcctggaaca cgtgtacaaa atccacaccc cctacaccat cctgatccac gccaagttca
     3961 ccgacagcaa gttcaacaag ctgaaggccg tgatgcagtt cctgcagggc acccacttcg
     4021 gcgacttcac cctgaagttc gtgtacaagg gcatggtgcc cagcaagaac gtggtcaagg
     4081 acagctgcca cgagtgtctg atcggccact accctggcgc gccgtcgacc tccatcacgg
     4141 cctataagag tgagggggag tcagcggagt tctccttccc actcaacctt ggagaggaaa
     4201 gcctgcaggg agagttgaga tggaaggcag agaaggctcc ttcttcccag tcctggatca
     4261 ccttctccct aaagaaccaa aaggtgtctg tgcagaagtc tactagcaac cccaagttcc
     4321 agctgtccga aacgctccca ctcacccttc agatacccca ggtctccctt cagtttgctg
     4381 gttctggcaa cctgaccctg actctggaca gagggatact gtatcaggaa gtgaacctgg
     4441 tggtgatgaa agtgactcag cccgacagca acactttgac ctgtgaggtg atgggaccca
     4501 cctcacccaa gatgagactg atcttgaagc aggagaatca ggaggccagg gtctccaggc
     4561 aggagaaagt gattcaagtg caggcccctg aagcaggggt gtggcaatgt ctactgagtg
     4621 aaggtgaaga ggtcaagatg gactccaaga tccaggtttt atccaaaggg ttgaattccg
     4681 gcagcctgca ccacatcctg gacgcccaga agatgctgtg gaaccacagg gacaggaacc
     4741 tgcctcctct ggcccctctg ggcccccacc accatcacca ccattgagtc tagacacgtg
     4801 attaattaag gatcccccga cctcgacctc tggctaataa aggaaattta ttttcattgc
     4861 aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca
     4921 tttggtcgag atccctcgga gatctctagc tagaggatcg atccccgccc cggacgaact
     4981 aaacctgact acgacatctc tgccccttct tcgcggggca gtgcatgtaa tcccttcagt
     5041 tggttggtac aacttgccaa ctgggccctg ttccacatgt gacacggggg gggaccaaac
     5101 acaaaggggt tctctgactg tagttgacat ccttataaat ggatgtgcac atttgccaac
     5161 actgagtggc tttcatcctg gagcagactt tgcagtctgt ggactgcaac acaacattgc
     5221 ctttatgtgt aactcttggc tgaagctctt acaccaatgc tgggggacat gtacctccca
     5281 ggggcccagg aagactacgg gaggctacac caacgtcaat cagaggggcc tgtgtagcta
     5341 ccgataagcg gaccctcaag agggcattag caatagtgtt tataaggccc ccttgttaac
     5401 cctaaacggg tagcatatgc ttcccgggta gtagtatata ctatccagac taaccctaat
     5461 tcaatagcat atgttaccca acgggaagca tatgctatcg aattagggtt agtaaaaggg
     5521 tcctaaggaa cagcgatatc tcccacccca tgagctgtca cggttttatt tacatggggt
     5581 caggattcca cgagggtagt gaaccatttt agtcacaagg gcagtggctg aagatcaagg
     5641 agcgggcagt gaactctcct gaatcttcgc ctgcttcttc attctccttc gtttagctaa
     5701 tagaataact gctgagttgt gaacagtaag gtgtatgtga ggtgctcgaa aacaaggttt
     5761 caggtgacgc ccccagaata aaatttggac ggggggttca gtggtggcat tgtgctatga
     5821 caccaatata accctcacaa accccttggg caataaatac tagtgtagga atgaaacatt
     5881 ctgaatatct ttaacaatag aaatccatgg ggtggggaca agccgtaaag actggatgtc
     5941 catctcacac gaatttatgg ctatgggcaa cacataatcc tagtgcaata tgatactggg
     6001 gttattaaga tgtgtcccag gcagggacca agacaggtga accatgttgt tacactctat
     6061 ttgtaacaag gggaaagaga gtggacgccg acagcagcgg actccactgg ttgtctctaa
     6121 cacccccgaa aattaaacgg ggctccacgc caatggggcc cataaacaaa gacaagtggc
     6181 cactcttttt tttgaaattg tggagtgggg gcacgcgtca gcccccacac gccgccctgc
     6241 ggttttggac tgtaaaataa gggtgtaata acttggctga ttgtaacccc gctaaccact
     6301 gcggtcaaac cacttgccca caaaaccact aatggcaccc cggggaatac ctgcataagt
     6361 aggtgggcgg gccaagatag gggcgcgatt gctgcgatct ggaggacaaa ttacacacac
     6421 ttgcgcctga gcgccaagca cagggttgtt ggtcctcata ttcacgaggt cgctgagagc
     6481 acggtgggct aatgttgcca tgggtagcat atactaccca aatatctgga tagcatatgc
     6541 tatcctaatc tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
     6601 tatcctaatc tatatctggg tagtatatgc tatcctaatt tatatctggg tagcataggc
     6661 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
     6721 tatcctaatc tgtatccggg tagcatatgc tatcctaata gagattaggg tagtatatgc
     6781 tatcctaatt tatatctggg tagcatatac tacccaaata tctggatagc atatgctatc
     6841 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagc ataggctatc
     6901 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc
     6961 ctaatttata tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc
     7021 ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc atatgctatc
     7081 ctcatgataa gctgtcaaac atgagaattt tcttgaagac gaaagggcct cgtgatacgc
     7141 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     7201 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     7261 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     7321 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     7381 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     7441 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     7501 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     7561 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     7621 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     7681 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     7741 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     7801 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     7861 gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     7921 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     7981 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     8041 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     8101 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     8161 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     8221 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     8281 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     8341 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     8401 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     8461 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     8521 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     8581 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     8641 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     8701 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     8761 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     8821 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     8881 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     8941 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     9001 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     9061 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     9121 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     9181 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     9241 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     9301 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagctctag
     9361 ctagaggtcg accaattctc atgtttgaca gcttatcatc gcagatccgg gcaacgttgt
     9421 tgccattgct gcaggcgcag aactggtagg tatggaagat ctatacattg aatcaatatt
     9481 ggcaattagc catattagtc attggttata tagcataaat caatattggc tattggccat
     9541 tgcatacgtt gtatctatat cataatatgt acatttatat tggctcatgt ccaatatgac
     9601 cgc
//
