LOCUS       sequence-21117-e         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG426GAL-Cerulean-ccdB
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     228..249
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(307..323)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(307..323)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(338..779)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(344..367)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    662..779
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat
       61 gtggtcgggg tagcgggcga agcactgcac gccccaggtc agggtggtca cgagggtggg
      121 ccagggcacg ggcagcttgc cggtggtgca gatgaacttc agggtcagct tgccgtaggt
      181 ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt cgccgtccag
      241 ctcgaccagg atgggcacca ccccggtgaa cagctcctcg cccttgctca ccattttgtc
      301 ccgggggatc cactagttct agaatccggg gttttttctc cttgacgtta aagtatagag
      361 gtatattaac aattttttgt tgatactttt attacatttg aataagaagt aatacaaacc
      421 gaaaatgttg aaagtattag ttaaagtggt tatgcagttt ttgcatttat atatctgtta
      481 atagatcaaa aatcatcgct tcgctgatta attaccccag aaataaggct aaaaaactaa
      541 tcgcattatc atcctatggt tgttaatttg attcgttcat ttgaaggttt gtggggccag
      601 gttactgcca atttttcctc ttcataacca taaaagctag tattgtagaa tctttattgt
      661 tcggagcagt gcggcgcgag gcacatctgc gtttcaggaa cgcgaccggt gaagacgagg
      721 acgcacggag gagagtcttc cttcggaggg ctgtcacccg ctcggcggct tctaatccgt
      781 actagagctc cagcttt
//
