LOCUS       sequence-211170-       10368 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length BMR1_03g04695 
            ectodomain with no N-linked glycans upon cotransfection with BirA in
            mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10368)
  AUTHORS   Elton CM, Rodriguez M, Ben Mamoun C, Lobo CA, Wright GJ
  TITLE     A library of recombinant Babesia microti cell surface and secreted 
            proteins for diagnostics discovery and reverse vaccinology.
  JOURNAL   Int J Parasitol. 2019 Feb;49(2):115-125. doi: 
            10.1016/j.ijpara.2018.10.003. Epub 2018 Oct 24.
  PUBMED    30367868
REFERENCE   2  (bases 1 to 10368)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ijpara.2018.10.003"; journalName: "Int J Parasitol"; 
            date: "2019-02"; volume: "49"; issue: "2"; pages: "115-125"
COMMENT     Sequence Label: BMR1_03g04695-bio-His
FEATURES             Location/Qualifiers
     source          1..10368
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5532..5549
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    5601..5656
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5655..5674)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7892..7910)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7978..8082
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8083..8151,8152..8943)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8301..8320)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9114..9702
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9603..9622
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9856..9873
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9990..10011
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10026..10031,10032..10049,10050..10056)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10064..10080
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10069..10091
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10088..10104
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10088..10104
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccg
     1201 acgatagcgg caatagcgcc cctcagaccc cccctgagac aagcagcccc atcaacggcg
     1261 tgatcggcga cgagaacaac ggcctggaac acctgagcag cagcggcctg gaagtggacg
     1321 acaacctgcc cgagctgctg aaaaccagcc ccttcagcgg ccagaacagc gacgtgcaga
     1381 gcgccagcac ccccgtggaa cctaccaccc ccgtgcacag caacgaccag agcaacccca
     1441 tcaccaacaa ggtggacacc aactccaacg accacaccga catcaagaac gagggcagca
     1501 gccaccggac cagcagcaac aatgccgccg tgaccaccaa caccaacaac gagatccgga
     1561 acggcggagg ccccctggac cagaacgagg acaaggccga ggacgagggc gagacagatg
     1621 ccgagggcag aggctggaac gagcggacca agaacaagcc caccttcaac gccgccaacc
     1681 gggacttcgt ggacgataat ctgcctgaac tgctcaagac ctccccattc tccggccaga
     1741 actccgatgt gcagtccgcc tctacccccg tcgagcccac cacacctgtg cactccaacg
     1801 atcagtccaa tcccattacc aacaaagtcg ataccaatag caatgaccac acagatatta
     1861 agaatgaggg gtccagccac cgcacctctt ccaacaacgc cgctgtcaca acaaatacca
     1921 acaatgagat taggaatggg ggcggacccc tcgatcagaa tgaagataag gccgaagatg
     1981 agggggaaac cgacgccgag ggacggggct ggaatgagag aacaaagaac aaacctactt
     2041 ttaatgccgc caacagagcc agccccgacg gcatcggcaa gatgaacatg gaagagaagc
     2101 agctcgagaa cttcatcaac gtggccagca acgccctgga actggacatc agcatcggcc
     2161 gggacaactt cgccaccaag tttctggccc agcacgtgaa catcttcggc gaccggatca
     2221 gcggcctgag cgccgcctac gtggaaggct acaacaacct ggccaagatc atgtacaaca
     2281 gccacagcgt gctgttcgac cggaagttca acggcgccgt gatcagcgac aacctgatcg
     2341 gcaatatcgc cgacttcggc agctacttcc tggaaatcag ccccaacacc gcccggacca
     2401 accgggccga ctacctgaag tccgtggtgc tgagcaaggt gcagtacctg ctgagcgccg
     2461 acttcagcac caccgacaac atccagcggc tgaccaacct ggccctggct ctgggctata
     2521 acaacgtgaa agagaacaac cccggcaaca gccagcacag catcaccacc agcctgagca
     2581 ccgagctgtt ctggtccttc ggcaacaaca tcttcctgtt cggccacctg gccaccctga
     2641 tgctggccta cctggaaagc aacgcctact tcaccagcgg cgccacccgg ccattcttca
     2701 gctggcagac cctggtgtcc accggcggca acgagaagtt cgacaagctg gacagcatgt
     2761 gcggcgtgat ccggggcagc aagtacagcc ggaagaacaa tggcttcatc aagccccact
     2821 acaagcggct gcggagaaag accctgctgg aaggcgagcc ccggctgctg tgcagcatgc
     2881 tggaagaggc cctggacacc gtggacaagg ccatcaagtt caagggcgag gaactgaaca
     2941 gccagggcgc caacatcgag aacagcgtgt ccaacgacat caacagcaag agactgcagg
     3001 ccaagctgtg ctccaacctg aacgacgccc tgatcaatgt ggcctgcgac ttcagaagca
     3061 gcaagctgga taagcacaac aagaagctga gagaggcctt cgacctgctg ctggcctgcg
     3121 gcaacctgaa caccggcaag aaagaggcct tccccgagta cctgcggctg atcagcaacc
     3181 ccttcgagta cggcgacatc ttcagcatga ccatgtggtg ggaccccaga gagttcgacg
     3241 gcaagcaggg ctgggtggaa atctataaga agctgcggaa gaacatcatg aagcccgagc
     3301 tgaagaacgt ggacatgcag ctgaagtacg acagcgccat cagctactac aagcagctga
     3361 aagagagcga aacctacccc aagaagaaca tcccctgggc cagactgtac ctgtacatga
     3421 gcgtgatcat gagccggtcc aacgccatga gctgggccga ggatgccctg cggagcttca
     3481 gcaacctgta ccggatgaag cccagcctgg tcatgcgggg cgagggcctg gaaaccctgc
     3541 tgaactactg cgcccctgac cccgtggccc tgagccatat ctttctgtac cacttcctga
     3601 ccaagaagga cgccggcaag gacctggaaa aggacctgcg gcggctggaa aagggcaccc
     3661 tgctgagccg gatcgtgaac agcgccagca tcttcatccc caacaagctg aagaaattcc
     3721 tgaagatggg cgccaggggc ttcttcaaca agaaactgaa caccctgcgg gccaagtcca
     3781 ccctgctgcg gctgttcccc aagaacctgc tgcacagcgc cctgggcgcc atcgagttca
     3841 ccacccacag cctggccaca ctgcagatca gcaagaacat ggatatgtgg gagagcctgg
     3901 cccagaccaa gaatctggac gctggcggct tccccggcga gatcgacagc ctgttcaacc
     3961 attggagcga gagcggcggc tactccggct acatcaccgg caagctggaa aacggcgacg
     4021 acctgaccgg cgacgatatc aagaagatga atatcaaggc ccccatcaac aacgatgccc
     4081 tgaactggca gaagtacatc aacaagaaga tcagcgagca cttcggcaag tttctgaacc
     4141 tgcccttcat ccaggccagc ggcagccaga agaactacat ctaccagctc gtgcgggaca
     4201 gcaaggccaa cctggacgac aatctggaac agaccgtgtt cttcggcaag gtgctgcccc
     4261 ctggcaagac caacaatgtg atcaaaaagc tgaagcggat cgccgacagc ttcacctcca
     4321 tgctgctgcg gagcagcgcc agacccgtgg atcatgccgt gtgggtcgga gtgaagatca
     4381 acgtgcccat cgtgatccac atcaccaaga aactgtacat gatccagcgg gacatgccca
     4441 gaaaagaggc ctggaacctg gaatccgcct tcctggacct gctgcaggac ctggtcatca
     4501 tggtcaccaa ccctggcaag cggagcccca tcggcttcga gacaatcggc ggcaaccccg
     4561 gcctgcccga gatcagcatc agataccccc acatgagcat cgaggaacgg aagatcgagt
     4621 tccagcacag ccagtgcgcc gaccactgca tcagcatctg gcggagcctg atcgccttca
     4681 ccctgaatac cctgaacaac cctgccgcca tcaagcagtt cgagaagtcc ctgtccagca
     4741 actccgccct gaacgacatg agcaagcccg agtacatcaa tagcttcaag tacatcctga
     4801 agggcgactc cgtgctgcat atgtacgaca acatgctgcc tcggaaagtg aagagagaga
     4861 tcaaggccct gaagtacggt ggcgcgccgt cgacctccat cacggcctat aagagtgagg
     4921 gggagtcagc ggagttctcc ttcccactca accttggaga ggaaagcctg cagggagagt
     4981 tgagatggaa ggcagagaag gctccttctt cccagtcctg gatcaccttc tccctaaaga
     5041 accaaaaggt gtctgtgcag aagtctacta gcaaccccaa gttccagctg tccgaaacgc
     5101 tcccactcac ccttcagata ccccaggtct cccttcagtt tgctggttct ggcaacctga
     5161 ccctgactct ggacagaggg atactgtatc aggaagtgaa cctggtggtg atgaaagtga
     5221 ctcagcccga cagcaacact ttgacctgtg aggtgatggg acccacctca cccaagatga
     5281 gactgatctt gaagcaggag aatcaggagg ccagggtctc caggcaggag aaagtgattc
     5341 aagtgcaggc ccctgaagca ggggtgtggc aatgtctact gagtgaaggt gaagaggtca
     5401 agatggactc caagatccag gttttatcca aagggttgaa ttccggcagc ctgcaccaca
     5461 tcctggacgc ccagaagatg ctgtggaacc acagggacag gaacctgcct cctctggccc
     5521 ctctgggccc ccaccaccat caccaccatt gagtctagac acgtgattaa ttaaggatcc
     5581 cccgacctcg acctctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
     5641 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttgg tcgagatccc
     5701 tcggagatct ctagctagag gatcgatccc cgccccggac gaactaaacc tgactacgac
     5761 atctctgccc cttcttcgcg gggcagtgca tgtaatccct tcagttggtt ggtacaactt
     5821 gccaactggg ccctgttcca catgtgacac ggggggggac caaacacaaa ggggttctct
     5881 gactgtagtt gacatcctta taaatggatg tgcacatttg ccaacactga gtggctttca
     5941 tcctggagca gactttgcag tctgtggact gcaacacaac attgccttta tgtgtaactc
     6001 ttggctgaag ctcttacacc aatgctgggg gacatgtacc tcccaggggc ccaggaagac
     6061 tacgggaggc tacaccaacg tcaatcagag gggcctgtgt agctaccgat aagcggaccc
     6121 tcaagagggc attagcaata gtgtttataa ggcccccttg ttaaccctaa acgggtagca
     6181 tatgcttccc gggtagtagt atatactatc cagactaacc ctaattcaat agcatatgtt
     6241 acccaacggg aagcatatgc tatcgaatta gggttagtaa aagggtccta aggaacagcg
     6301 atatctccca ccccatgagc tgtcacggtt ttatttacat ggggtcagga ttccacgagg
     6361 gtagtgaacc attttagtca caagggcagt ggctgaagat caaggagcgg gcagtgaact
     6421 ctcctgaatc ttcgcctgct tcttcattct ccttcgttta gctaatagaa taactgctga
     6481 gttgtgaaca gtaaggtgta tgtgaggtgc tcgaaaacaa ggtttcaggt gacgccccca
     6541 gaataaaatt tggacggggg gttcagtggt ggcattgtgc tatgacacca atataaccct
     6601 cacaaacccc ttgggcaata aatactagtg taggaatgaa acattctgaa tatctttaac
     6661 aatagaaatc catggggtgg ggacaagccg taaagactgg atgtccatct cacacgaatt
     6721 tatggctatg ggcaacacat aatcctagtg caatatgata ctggggttat taagatgtgt
     6781 cccaggcagg gaccaagaca ggtgaaccat gttgttacac tctatttgta acaaggggaa
     6841 agagagtgga cgccgacagc agcggactcc actggttgtc tctaacaccc ccgaaaatta
     6901 aacggggctc cacgccaatg gggcccataa acaaagacaa gtggccactc ttttttttga
     6961 aattgtggag tgggggcacg cgtcagcccc cacacgccgc cctgcggttt tggactgtaa
     7021 aataagggtg taataacttg gctgattgta accccgctaa ccactgcggt caaaccactt
     7081 gcccacaaaa ccactaatgg caccccgggg aatacctgca taagtaggtg ggcgggccaa
     7141 gataggggcg cgattgctgc gatctggagg acaaattaca cacacttgcg cctgagcgcc
     7201 aagcacaggg ttgttggtcc tcatattcac gaggtcgctg agagcacggt gggctaatgt
     7261 tgccatgggt agcatatact acccaaatat ctggatagca tatgctatcc taatctatat
     7321 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     7381 ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat
     7441 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat
     7501 ccgggtagca tatgctatcc taatagagat tagggtagta tatgctatcc taatttatat
     7561 ctgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat ctatatctgg
     7621 gtagcatatg ctatcctaat ctatatctgg gtagcatagg ctatcctaat ctatatctgg
     7681 gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat ttatatctgg
     7741 gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     7801 gtagtatatg ctatcctaat ctgtatccgg gtagcatatg ctatcctcat gataagctgt
     7861 caaacatgag aattttcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt
     7921 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     7981 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     8041 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     8101 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     8161 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     8221 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     8281 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa
     8341 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     8401 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     8461 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     8521 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     8581 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca
     8641 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     8701 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     8761 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     8821 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     8881 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     8941 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     9001 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     9061 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     9121 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     9181 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     9241 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     9301 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     9361 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     9421 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     9481 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     9541 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     9601 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     9661 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     9721 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     9781 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     9841 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     9901 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     9961 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
    10021 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
    10081 tttcacacag gaaacagcta tgaccatgat tacgccaagc tctagctaga ggtcgaccaa
    10141 ttctcatgtt tgacagctta tcatcgcaga tccgggcaac gttgttgcca ttgctgcagg
    10201 cgcagaactg gtaggtatgg aagatctata cattgaatca atattggcaa ttagccatat
    10261 tagtcattgg ttatatagca taaatcaata ttggctattg gccattgcat acgttgtatc
    10321 tatatcataa tatgtacatt tatattggct catgtccaat atgaccgc
//
