LOCUS       sequence-211173-        8622 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length BMR1_01g03335 
            ectodomain with no N-linked glycans upon cotransfection with BirA in
            mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8622)
  AUTHORS   Elton CM, Rodriguez M, Ben Mamoun C, Lobo CA, Wright GJ
  TITLE     A library of recombinant Babesia microti cell surface and secreted 
            proteins for diagnostics discovery and reverse vaccinology.
  JOURNAL   Int J Parasitol. 2019 Feb;49(2):115-125. doi: 
            10.1016/j.ijpara.2018.10.003. Epub 2018 Oct 24.
  PUBMED    30367868
REFERENCE   2  (bases 1 to 8622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ijpara.2018.10.003"; journalName: "Int J Parasitol"; 
            date: "2019-02"; volume: "49"; issue: "2"; pages: "115-125"
COMMENT     Sequence Label: BMR1_01g03335-bio-His
FEATURES             Location/Qualifiers
     source          1..8622
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3786..3803
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3855..3910
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3909..3928)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6146..6164)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6232..6336
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6337..6405,6406..7197)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6555..6574)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7368..7956
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7857..7876
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8110..8127
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8244..8265
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8280..8285,8286..8303,8304..8310)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8318..8334
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8323..8345
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8342..8358
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8342..8358
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccg
     1201 agtttgagtc tggcggcctg aagctggacc tgatcggcga ggtggccgac tacggcgact
     1261 tctacggcaa gaacgagatc aatatcttca ccttcctgta ctacaagagc aacgacctgt
     1321 acaagaacac cgtgatcgtg tacagcctgg acaagaagaa gaacaaatac gagatcagct
     1381 acatgttcga gttcgagcac cagggctttt gcgagggcat gatcgtgacc gactggacca
     1441 tggacagccg ggtggacgcc ctgctgtacc tgcgggacga gaagggcatc tacatcgtgg
     1501 gcctggccca gagcgccccc atcgacgagg tggaattctc tatcgcctgg accagccggg
     1561 atctgatcgg cccctacctg cagcacgaga acggcaacat ccagctggcc cccgaggaaa
     1621 gccggcacaa cagcaacatc caccccctgc tgtgcgacat caacggcgac ggcagagtgg
     1681 acatgatcac ccagggccac gacggcaccc gggtcgtgtg gatcaacgac ggcaaggtgt
     1741 tcatccccta cgcctgggac gacgtgccca acgtccgctt cgtgaagtac gacctgggca
     1801 tgctgcagga cgaggaccag aacgagaacg tgggcggcaa caccgacaac gtgcagctgc
     1861 tgaatatcgc cgtgccccac agcaacggct tcgtggacat caatggcgat tgcagacccg
     1921 acctgctgtt caccgtgggc accgataatg tgcacatgat tgagatctgg ctgagcgacg
     1981 tggaagtggg cgagaatgtg gtggtcacca actaccggca gatcgagaag ctgcagctgc
     2041 ccgtgcaccc cggccagctg atcttcgccg atttcaatgg cgacggctcc atcgacatcg
     2101 gcatccccag ctgcatcccc gacgagggca gcaagaactg catcagcagc agcttcaaca
     2161 tcatgttcaa ccaccagcag cccctgtgca acaacctgtg gaaggacagc accgtgaagt
     2221 gccggaagcc caccgagctg tgcaccaaga cccccttcga gatcagcaag taccaggtgg
     2281 tcaagttcga caacagcgag gtgtccggcg ccaacgactt cgtgtacatg aacaacgcca
     2341 acttcggcaa tatcctgcgg ctggcctacg gcgatttcga cctggacggc taccccgacc
     2401 tggcctttat cgccagcaac gtggccagcc ccgagcccag catcgtggtg tacaagaata
     2461 tgctgttcga caaggacagc cgcttcgtgc tggtcaagag catccccatc gagaactcta
     2521 agtacgacta ccggctgagc ttcttcgacg tgttcgagga cggcgacctg aacgtgatcc
     2581 tgttcggcct gcccaacgag gaacagctgg gcaacaacat cttcgtgaag gccaacgagg
     2641 acgagagccc caaggacggc gagaactgcg aggaaaagaa ggacaagaaa gaatacgaca
     2701 tgagcaaata cgacatcaag tacctgccct tcgagagcta caagtggaac gtgaaagagt
     2761 tcggcctgtt cctgaaggcc agcagcatcg gccccctgag ccacatccat tttcaggacg
     2821 acatcaactt cgagacaaac atccacggcc ccgtgttcgt gatcaccgtg tccgatatct
     2881 acggcgagaa ggcccccaga caggccatcc agaggcccct gagcggctac ctgcccctgc
     2941 agctccccta cgtgatcttc ggcctgggca acaccaacaa ttacgtggaa gagttctacc
     3001 tgggaatgcc cagcgccagc cacgactaca aggacatgtg gatcagcatc atccccaact
     3061 gctttgtgat cgccgtgacc ttccccctgg acatcccctc caagtggaat ctgaagctga
     3121 gcatcaaccc cgctggcgcg ccgtcgacct ccatcacggc ctataagagt gagggggagt
     3181 cagcggagtt ctccttccca ctcaaccttg gagaggaaag cctgcaggga gagttgagat
     3241 ggaaggcaga gaaggctcct tcttcccagt cctggatcac cttctcccta aagaaccaaa
     3301 aggtgtctgt gcagaagtct actagcaacc ccaagttcca gctgtccgaa acgctcccac
     3361 tcacccttca gataccccag gtctcccttc agtttgctgg ttctggcaac ctgaccctga
     3421 ctctggacag agggatactg tatcaggaag tgaacctggt ggtgatgaaa gtgactcagc
     3481 ccgacagcaa cactttgacc tgtgaggtga tgggacccac ctcacccaag atgagactga
     3541 tcttgaagca ggagaatcag gaggccaggg tctccaggca ggagaaagtg attcaagtgc
     3601 aggcccctga agcaggggtg tggcaatgtc tactgagtga aggtgaagag gtcaagatgg
     3661 actccaagat ccaggtttta tccaaagggt tgaattccgg cagcctgcac cacatcctgg
     3721 acgcccagaa gatgctgtgg aaccacaggg acaggaacct gcctcctctg gcccctctgg
     3781 gcccccacca ccatcaccac cattgagtct agacacgtga ttaattaagg atcccccgac
     3841 ctcgacctct ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt
     3901 gtgtctctca ctcggaagga catatgggag ggcaaatcat ttggtcgaga tccctcggag
     3961 atctctagct agaggatcga tccccgcccc ggacgaacta aacctgacta cgacatctct
     4021 gccccttctt cgcggggcag tgcatgtaat cccttcagtt ggttggtaca acttgccaac
     4081 tgggccctgt tccacatgtg acacgggggg ggaccaaaca caaaggggtt ctctgactgt
     4141 agttgacatc cttataaatg gatgtgcaca tttgccaaca ctgagtggct ttcatcctgg
     4201 agcagacttt gcagtctgtg gactgcaaca caacattgcc tttatgtgta actcttggct
     4261 gaagctctta caccaatgct gggggacatg tacctcccag gggcccagga agactacggg
     4321 aggctacacc aacgtcaatc agaggggcct gtgtagctac cgataagcgg accctcaaga
     4381 gggcattagc aatagtgttt ataaggcccc cttgttaacc ctaaacgggt agcatatgct
     4441 tcccgggtag tagtatatac tatccagact aaccctaatt caatagcata tgttacccaa
     4501 cgggaagcat atgctatcga attagggtta gtaaaagggt cctaaggaac agcgatatct
     4561 cccaccccat gagctgtcac ggttttattt acatggggtc aggattccac gagggtagtg
     4621 aaccatttta gtcacaaggg cagtggctga agatcaagga gcgggcagtg aactctcctg
     4681 aatcttcgcc tgcttcttca ttctccttcg tttagctaat agaataactg ctgagttgtg
     4741 aacagtaagg tgtatgtgag gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa
     4801 aatttggacg gggggttcag tggtggcatt gtgctatgac accaatataa ccctcacaaa
     4861 ccccttgggc aataaatact agtgtaggaa tgaaacattc tgaatatctt taacaataga
     4921 aatccatggg gtggggacaa gccgtaaaga ctggatgtcc atctcacacg aatttatggc
     4981 tatgggcaac acataatcct agtgcaatat gatactgggg ttattaagat gtgtcccagg
     5041 cagggaccaa gacaggtgaa ccatgttgtt acactctatt tgtaacaagg ggaaagagag
     5101 tggacgccga cagcagcgga ctccactggt tgtctctaac acccccgaaa attaaacggg
     5161 gctccacgcc aatggggccc ataaacaaag acaagtggcc actctttttt ttgaaattgt
     5221 ggagtggggg cacgcgtcag cccccacacg ccgccctgcg gttttggact gtaaaataag
     5281 ggtgtaataa cttggctgat tgtaaccccg ctaaccactg cggtcaaacc acttgcccac
     5341 aaaaccacta atggcacccc ggggaatacc tgcataagta ggtgggcggg ccaagatagg
     5401 ggcgcgattg ctgcgatctg gaggacaaat tacacacact tgcgcctgag cgccaagcac
     5461 agggttgttg gtcctcatat tcacgaggtc gctgagagca cggtgggcta atgttgccat
     5521 gggtagcata tactacccaa atatctggat agcatatgct atcctaatct atatctgggt
     5581 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     5641 agtatatgct atcctaattt atatctgggt agcataggct atcctaatct atatctgggt
     5701 agcatatgct atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt
     5761 agcatatgct atcctaatag agattagggt agtatatgct atcctaattt atatctgggt
     5821 agcatatact acccaaatat ctggatagca tatgctatcc taatctatat ctgggtagca
     5881 tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat ctgggtagca
     5941 tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca
     6001 taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta
     6061 tatgctatcc taatctgtat ccgggtagca tatgctatcc tcatgataag ctgtcaaaca
     6121 tgagaatttt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     6181 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     6241 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     6301 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     6361 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     6421 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     6481 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     6541 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa
     6601 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     6661 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     6721 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     6781 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     6841 gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg
     6901 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     6961 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     7021 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     7081 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     7141 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     7201 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     7261 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     7321 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     7381 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     7441 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     7501 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     7561 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     7621 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     7681 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     7741 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     7801 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     7861 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     7921 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     7981 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     8041 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     8101 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     8161 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     8221 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     8281 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     8341 acaggaaaca gctatgacca tgattacgcc aagctctagc tagaggtcga ccaattctca
     8401 tgtttgacag cttatcatcg cagatccggg caacgttgtt gccattgctg caggcgcaga
     8461 actggtaggt atggaagatc tatacattga atcaatattg gcaattagcc atattagtca
     8521 ttggttatat agcataaatc aatattggct attggccatt gcatacgttg tatctatatc
     8581 ataatatgta catttatatt ggctcatgtc caatatgacc gc
//
