LOCUS       sequence-211184-        2605 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Suicide vector based on the TetR/Pip OFF system. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Arnold FM, Hohl M, Remm S, Koliwer-Brandl H, Adenau S, Chusri S, 
            Sander P, Hilbi H, Seeger MA
  TITLE     A uniform cloning platform for mycobacterial genetics and protein 
            production.
  JOURNAL   Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.1038/s41598-018-27687-5.
  PUBMED    29934571
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2018-06-22"; volume: "8(1):9539. doi"; pages: " 
            10.1038/s41598-018-27687-5"
COMMENT     Sequence Label: pCOND (Apr)
FEATURES             Location/Qualifiers
     source          1..2605
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(260..277)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(431..1019)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(511..530)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1623..2423)
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
ORIGIN
        1 ctcaacctcg aagcgtgtgg ttgcggagcc atctagcaac cacacgagga tccagatctc
       61 catcctgacg gatggcctta cgagttccga gatcaccccg gtcacggtgg acggcgtcac
      121 cttctacgac ctgaccgcgg agaaatagcg ctgtacagcg tatgggaatc tcttgtacgg
      181 tgtacgagta tcttcccgta caccgtacaa ggagcctgaa tgagttgaag agcggatccg
      241 ctcttcagca ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
      301 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
      361 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
      421 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
      481 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
      541 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
      601 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
      661 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
      721 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
      781 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
      841 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
      901 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
      961 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     1021 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     1081 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     1141 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     1201 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     1261 tccccgacgt ggccgaccag cccgtcatcg tcaacgcctg atccgcggtg cggacaggcc
     1321 gtgtcgcgac cggccgtgcg gaattaagcc ggcccgtacc ctgtgaatag aggtccgctg
     1381 tgacacaaga atccctgtta cttctcgacc gtattgattc ggatgattcc tacgcgagcc
     1441 tgtaccatgg atcagccgtc caaatgcggg attttcgtta gtcggaggcc aaacggcatt
     1501 gagcgtcagc atatcatcag cgagctgaag aaagacaatc cccgatccgc tccacgtgtt
     1561 gccccagcaa tcagcgcgac cttgcccctc caacgtcatc tcgttctccg ctcatgagct
     1621 cagccaatcg actggcgagc ggcatcgcat tcttcgcatc ccgcctctgg cggatgcagg
     1681 aagatcaacg gatctcggcc cagttgaccc agggctgtcg ccacaatgtc gcgggagcgg
     1741 atcaaccgag caaaggcatg accgactgga ccttccttct gaaggctctt ttccttgagc
     1801 cacctgtccg ccaaggcaaa gcgctcacag cagtggtcat tctcgagata atcgacgcgt
     1861 accaacttgc catcctgaag aatggtgcag tgtctcggca ccccataggg aacctttgcc
     1921 atcaactcgg caagatgcag cgtcgtgttg gcatcgtgtc ccacgccgag gagaagtacc
     1981 tgcccatcga gttcatggac acgggcgacc gggcttgcag gcgagtgagg tggcaggggc
     2041 aatggatcag agatgatctg ctctgcctgt ggccccgctg ccgcaaaggc aaatggatgg
     2101 gcgctgcgct ttacatttgg caggcgccag aatgtgtcag agacaactcc aaggtccggt
     2161 gtaacgggcg acgtggcagg atcgaacggc tcgtcgtcca gacctgacca cgagggcatg
     2221 acgagcgtcc ctcccggacc cagcgcagca cgcagggcct cgatcagtcc aagtggccca
     2281 tcttcgaggg gccggacgct acggaaggag ctgtggacca gcagcacacc gccgggggta
     2341 accccaaggt tgagaagctg accgatgagc tcggcttttc gccattcgta ttgcacgaca
     2401 ttgcactcca ccgctgatga catcagtcga tcatagcacg atcaacggca ctgttgcaaa
     2461 tagtcggtgg tgataaactt atcatcccct tttgctgatg gagctgcaca tgaaccaaaa
     2521 ggatctaggt gaagctcctt ctagatacat caacgaggac agtcgcacga cgaagttctt
     2581 ctggatcgcg cccgtgctgg aagca
//
