LOCUS       sequence-211186-        7188 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length BMR1_04g09190 
            ectodomain with no N-linked glycans upon cotransfection with BirA in
            mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7188)
  AUTHORS   Elton CM, Rodriguez M, Ben Mamoun C, Lobo CA, Wright GJ
  TITLE     A library of recombinant Babesia microti cell surface and secreted 
            proteins for diagnostics discovery and reverse vaccinology.
  JOURNAL   Int J Parasitol. 2019 Feb;49(2):115-125. doi: 
            10.1016/j.ijpara.2018.10.003. Epub 2018 Oct 24.
  PUBMED    30367868
REFERENCE   2  (bases 1 to 7188)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ijpara.2018.10.003"; journalName: "Int J Parasitol"; 
            date: "2019-02"; volume: "49"; issue: "2"; pages: "115-125"
COMMENT     Sequence Label: BMR1_04g09190-bio-His
FEATURES             Location/Qualifiers
     source          1..7188
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2352..2369
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2421..2476
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2475..2494)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4712..4730)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4798..4902
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4903..4971,4972..5763)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5121..5140)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5934..6522
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6423..6442
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6676..6693
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6810..6831
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6846..6851,6852..6869,6870..6876)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6884..6900
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6889..6911
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6908..6924
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6908..6924
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccg
     1201 aggaatgccc tagcgatcac ctgtgcaagg gcagatgcga actcgtgggc aacttcaaga
     1261 tctgcatctg caaccccggc gagacaaact gggactgcaa gctggacctg gacctgtgca
     1321 accgggactg cgtgatcggc ggcaccgaca accagtgcac caagctgctg tgcaacaagg
     1381 gcacctgtaa caacgccctg acctacccct tctacagctg caactgcggc cctttctaca
     1441 ccggccagaa ctgccagatc gagaacaacc cctgcatcct gagcaacgcc aacccctgct
     1501 ccaacggaag atgcgagttc atccacggca tcaacaaagt gaactgccac tgcaaccctg
     1561 gctggaaggc ccgggacagc gagactaact acgccatcga ctggggcagc gccaaagtga
     1621 tcatcgaccc cccctgcagc gaacaggtgt tctacggcat cagcaagatc gcccccagac
     1681 tgagccaggg caacagagtg ggcgcgccgt cgacctccat cacggcctat aagagtgagg
     1741 gggagtcagc ggagttctcc ttcccactca accttggaga ggaaagcctg cagggagagt
     1801 tgagatggaa ggcagagaag gctccttctt cccagtcctg gatcaccttc tccctaaaga
     1861 accaaaaggt gtctgtgcag aagtctacta gcaaccccaa gttccagctg tccgaaacgc
     1921 tcccactcac ccttcagata ccccaggtct cccttcagtt tgctggttct ggcaacctga
     1981 ccctgactct ggacagaggg atactgtatc aggaagtgaa cctggtggtg atgaaagtga
     2041 ctcagcccga cagcaacact ttgacctgtg aggtgatggg acccacctca cccaagatga
     2101 gactgatctt gaagcaggag aatcaggagg ccagggtctc caggcaggag aaagtgattc
     2161 aagtgcaggc ccctgaagca ggggtgtggc aatgtctact gagtgaaggt gaagaggtca
     2221 agatggactc caagatccag gttttatcca aagggttgaa ttccggcagc ctgcaccaca
     2281 tcctggacgc ccagaagatg ctgtggaacc acagggacag gaacctgcct cctctggccc
     2341 ctctgggccc ccaccaccat caccaccatt gagtctagac acgtgattaa ttaaggatcc
     2401 cccgacctcg acctctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
     2461 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttgg tcgagatccc
     2521 tcggagatct ctagctagag gatcgatccc cgccccggac gaactaaacc tgactacgac
     2581 atctctgccc cttcttcgcg gggcagtgca tgtaatccct tcagttggtt ggtacaactt
     2641 gccaactggg ccctgttcca catgtgacac ggggggggac caaacacaaa ggggttctct
     2701 gactgtagtt gacatcctta taaatggatg tgcacatttg ccaacactga gtggctttca
     2761 tcctggagca gactttgcag tctgtggact gcaacacaac attgccttta tgtgtaactc
     2821 ttggctgaag ctcttacacc aatgctgggg gacatgtacc tcccaggggc ccaggaagac
     2881 tacgggaggc tacaccaacg tcaatcagag gggcctgtgt agctaccgat aagcggaccc
     2941 tcaagagggc attagcaata gtgtttataa ggcccccttg ttaaccctaa acgggtagca
     3001 tatgcttccc gggtagtagt atatactatc cagactaacc ctaattcaat agcatatgtt
     3061 acccaacggg aagcatatgc tatcgaatta gggttagtaa aagggtccta aggaacagcg
     3121 atatctccca ccccatgagc tgtcacggtt ttatttacat ggggtcagga ttccacgagg
     3181 gtagtgaacc attttagtca caagggcagt ggctgaagat caaggagcgg gcagtgaact
     3241 ctcctgaatc ttcgcctgct tcttcattct ccttcgttta gctaatagaa taactgctga
     3301 gttgtgaaca gtaaggtgta tgtgaggtgc tcgaaaacaa ggtttcaggt gacgccccca
     3361 gaataaaatt tggacggggg gttcagtggt ggcattgtgc tatgacacca atataaccct
     3421 cacaaacccc ttgggcaata aatactagtg taggaatgaa acattctgaa tatctttaac
     3481 aatagaaatc catggggtgg ggacaagccg taaagactgg atgtccatct cacacgaatt
     3541 tatggctatg ggcaacacat aatcctagtg caatatgata ctggggttat taagatgtgt
     3601 cccaggcagg gaccaagaca ggtgaaccat gttgttacac tctatttgta acaaggggaa
     3661 agagagtgga cgccgacagc agcggactcc actggttgtc tctaacaccc ccgaaaatta
     3721 aacggggctc cacgccaatg gggcccataa acaaagacaa gtggccactc ttttttttga
     3781 aattgtggag tgggggcacg cgtcagcccc cacacgccgc cctgcggttt tggactgtaa
     3841 aataagggtg taataacttg gctgattgta accccgctaa ccactgcggt caaaccactt
     3901 gcccacaaaa ccactaatgg caccccgggg aatacctgca taagtaggtg ggcgggccaa
     3961 gataggggcg cgattgctgc gatctggagg acaaattaca cacacttgcg cctgagcgcc
     4021 aagcacaggg ttgttggtcc tcatattcac gaggtcgctg agagcacggt gggctaatgt
     4081 tgccatgggt agcatatact acccaaatat ctggatagca tatgctatcc taatctatat
     4141 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     4201 ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat
     4261 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat
     4321 ccgggtagca tatgctatcc taatagagat tagggtagta tatgctatcc taatttatat
     4381 ctgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat ctatatctgg
     4441 gtagcatatg ctatcctaat ctatatctgg gtagcatagg ctatcctaat ctatatctgg
     4501 gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat ttatatctgg
     4561 gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     4621 gtagtatatg ctatcctaat ctgtatccgg gtagcatatg ctatcctcat gataagctgt
     4681 caaacatgag aattttcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt
     4741 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     4801 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     4861 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     4921 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     4981 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     5041 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     5101 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa
     5161 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     5221 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     5281 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     5341 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     5401 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca
     5461 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     5521 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     5581 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     5641 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     5701 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     5761 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     5821 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     5881 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     5941 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     6001 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     6061 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     6121 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     6181 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     6241 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     6301 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     6361 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     6421 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     6481 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     6541 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     6601 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     6661 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     6721 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     6781 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     6841 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     6901 tttcacacag gaaacagcta tgaccatgat tacgccaagc tctagctaga ggtcgaccaa
     6961 ttctcatgtt tgacagctta tcatcgcaga tccgggcaac gttgttgcca ttgctgcagg
     7021 cgcagaactg gtaggtatgg aagatctata cattgaatca atattggcaa ttagccatat
     7081 tagtcattgg ttatatagca taaatcaata ttggctattg gccattgcat acgttgtatc
     7141 tatatcataa tatgtacatt tatattggct catgtccaat atgaccgc
//
