LOCUS       sequence-211187-        8037 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length BMR1_03g02875 
            ectodomain with no N-linked glycans upon cotransfection with BirA in
            mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8037)
  AUTHORS   Elton CM, Rodriguez M, Ben Mamoun C, Lobo CA, Wright GJ
  TITLE     A library of recombinant Babesia microti cell surface and secreted 
            proteins for diagnostics discovery and reverse vaccinology.
  JOURNAL   Int J Parasitol. 2019 Feb;49(2):115-125. doi: 
            10.1016/j.ijpara.2018.10.003. Epub 2018 Oct 24.
  PUBMED    30367868
REFERENCE   2  (bases 1 to 8037)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ijpara.2018.10.003"; journalName: "Int J Parasitol"; 
            date: "2019-02"; volume: "49"; issue: "2"; pages: "115-125"
COMMENT     Sequence Label: BMR1_03g02875-bio-His
FEATURES             Location/Qualifiers
     source          1..8037
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3201..3218
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3270..3325
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3324..3343)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5561..5579)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5647..5751
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5752..5820,5821..6612)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5970..5989)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6783..7371
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7272..7291
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7525..7542
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7659..7680
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7695..7700,7701..7718,7719..7725)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7733..7749
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7738..7760
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7757..7773
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7757..7773
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcct
     1201 atgagtgcaa gctggtgctg gaactggacc gcgagcccac caacatgatc aagatcgact
     1261 actacatgag caagctgtac gtgttcccca gccccaacat ccccatcggc accctgatca
     1321 gcgagatcaa ctggggcgac atcttccact tcaagatcaa caagaactac ctgctggcct
     1381 ggatctacct gaagcccatg ggcaacaacg ccctggaact gggcatcaag ctgctggaca
     1441 tcagcggcaa caagttcatc gagacacccc atctgctgat cttccccgag ttctacaact
     1501 tcccccagtg gctgaacctg aacagccccc agctgatcaa ccggatccgg aagtccatcg
     1561 tggacgtgaa cctggactcc ctgcccgcca acctggacgt ggtgtacacc cgggacttcg
     1621 tgaccttcaa gaccaacggc gagagcagca tcggcaactt cgcctgggac aacttcatcg
     1681 tggccaacct gatcaaccac aacttcgcca acatcatcaa gctgaccccc gtggcctacc
     1741 ggaagatcaa gatcaccgcc tacaacagcc tgatccccga cagcatctac cacgtgggca
     1801 ccatcatctt ccctgaagag tacatcttcc catattggtt cgagcacgtg aacggctgct
     1861 tctacaagct gatcaccaag cagttcaaga gcctgaacgt ggacgtgctg agcaccatct
     1921 tcagcgacga gatcctggtg cacatgcacc tgaacatgtt ccacatctac gtcaagcccg
     1981 agtacatcac ctgtgccgcc atcaagagca tcagcctgta caactacaag tacaacagca
     2041 tcagcagctt cgacgggatc gaccagatct ttctgtacaa gaagatcaac agcatcgaga
     2101 tcaccctggc cctgagcgag cacaacggca agtttatcaa gctgcacagc aagctgccct
     2161 acaacggcga catcaagccc gactgggcca ccgagctgag caagcacgcc accagcagcg
     2221 acgagctgat cgccctggac atcgacctga gcagcgacca ccccctgaaa gaaatcaaga
     2281 tgaccaagaa catgaacaag tacatcctcg agcccaagct ggaaggccac atcgtgttta
     2341 tcgccctgag caccagcgac accttctacc agatcgtgaa gccccctgcc cagatcaaga
     2401 agattatcat cacccccctg ctgtgctacc ggtggaagat taagatcgaa gtgaacgacc
     2461 ccaacagcaa gacctacacc gagcggtggc ggttcttcca gggcgagaac ctggtcaagt
     2521 tcctgaccta cgccaacccc aagaactctg gcgcgccgtc gacctccatc acggcctata
     2581 agagtgaggg ggagtcagcg gagttctcct tcccactcaa ccttggagag gaaagcctgc
     2641 agggagagtt gagatggaag gcagagaagg ctccttcttc ccagtcctgg atcaccttct
     2701 ccctaaagaa ccaaaaggtg tctgtgcaga agtctactag caaccccaag ttccagctgt
     2761 ccgaaacgct cccactcacc cttcagatac cccaggtctc ccttcagttt gctggttctg
     2821 gcaacctgac cctgactctg gacagaggga tactgtatca ggaagtgaac ctggtggtga
     2881 tgaaagtgac tcagcccgac agcaacactt tgacctgtga ggtgatggga cccacctcac
     2941 ccaagatgag actgatcttg aagcaggaga atcaggaggc cagggtctcc aggcaggaga
     3001 aagtgattca agtgcaggcc cctgaagcag gggtgtggca atgtctactg agtgaaggtg
     3061 aagaggtcaa gatggactcc aagatccagg ttttatccaa agggttgaat tccggcagcc
     3121 tgcaccacat cctggacgcc cagaagatgc tgtggaacca cagggacagg aacctgcctc
     3181 ctctggcccc tctgggcccc caccaccatc accaccattg agtctagaca cgtgattaat
     3241 taaggatccc ccgacctcga cctctggcta ataaaggaaa tttattttca ttgcaatagt
     3301 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttggt
     3361 cgagatccct cggagatctc tagctagagg atcgatcccc gccccggacg aactaaacct
     3421 gactacgaca tctctgcccc ttcttcgcgg ggcagtgcat gtaatccctt cagttggttg
     3481 gtacaacttg ccaactgggc cctgttccac atgtgacacg gggggggacc aaacacaaag
     3541 gggttctctg actgtagttg acatccttat aaatggatgt gcacatttgc caacactgag
     3601 tggctttcat cctggagcag actttgcagt ctgtggactg caacacaaca ttgcctttat
     3661 gtgtaactct tggctgaagc tcttacacca atgctggggg acatgtacct cccaggggcc
     3721 caggaagact acgggaggct acaccaacgt caatcagagg ggcctgtgta gctaccgata
     3781 agcggaccct caagagggca ttagcaatag tgtttataag gcccccttgt taaccctaaa
     3841 cgggtagcat atgcttcccg ggtagtagta tatactatcc agactaaccc taattcaata
     3901 gcatatgtta cccaacggga agcatatgct atcgaattag ggttagtaaa agggtcctaa
     3961 ggaacagcga tatctcccac cccatgagct gtcacggttt tatttacatg gggtcaggat
     4021 tccacgaggg tagtgaacca ttttagtcac aagggcagtg gctgaagatc aaggagcggg
     4081 cagtgaactc tcctgaatct tcgcctgctt cttcattctc cttcgtttag ctaatagaat
     4141 aactgctgag ttgtgaacag taaggtgtat gtgaggtgct cgaaaacaag gtttcaggtg
     4201 acgcccccag aataaaattt ggacgggggg ttcagtggtg gcattgtgct atgacaccaa
     4261 tataaccctc acaaacccct tgggcaataa atactagtgt aggaatgaaa cattctgaat
     4321 atctttaaca atagaaatcc atggggtggg gacaagccgt aaagactgga tgtccatctc
     4381 acacgaattt atggctatgg gcaacacata atcctagtgc aatatgatac tggggttatt
     4441 aagatgtgtc ccaggcaggg accaagacag gtgaaccatg ttgttacact ctatttgtaa
     4501 caaggggaaa gagagtggac gccgacagca gcggactcca ctggttgtct ctaacacccc
     4561 cgaaaattaa acggggctcc acgccaatgg ggcccataaa caaagacaag tggccactct
     4621 tttttttgaa attgtggagt gggggcacgc gtcagccccc acacgccgcc ctgcggtttt
     4681 ggactgtaaa ataagggtgt aataacttgg ctgattgtaa ccccgctaac cactgcggtc
     4741 aaaccacttg cccacaaaac cactaatggc accccgggga atacctgcat aagtaggtgg
     4801 gcgggccaag ataggggcgc gattgctgcg atctggagga caaattacac acacttgcgc
     4861 ctgagcgcca agcacagggt tgttggtcct catattcacg aggtcgctga gagcacggtg
     4921 ggctaatgtt gccatgggta gcatatacta cccaaatatc tggatagcat atgctatcct
     4981 aatctatatc tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct
     5041 aatctatatc tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct
     5101 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct
     5161 aatctgtatc cgggtagcat atgctatcct aatagagatt agggtagtat atgctatcct
     5221 aatttatatc tgggtagcat atactaccca aatatctgga tagcatatgc tatcctaatc
     5281 tatatctggg tagcatatgc tatcctaatc tatatctggg tagcataggc tatcctaatc
     5341 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatt
     5401 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     5461 tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc tatcctcatg
     5521 ataagctgtc aaacatgaga attttcttga agacgaaagg gcctcgtgat acgcctattt
     5581 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     5641 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     5701 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     5761 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     5821 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     5881 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     5941 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     6001 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     6061 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     6121 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     6181 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     6241 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca
     6301 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     6361 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     6421 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     6481 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     6541 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     6601 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     6661 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     6721 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     6781 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     6841 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     6901 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     6961 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     7021 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     7081 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     7141 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     7201 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     7261 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     7321 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     7381 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     7441 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     7501 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     7561 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     7621 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     7681 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     7741 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct ctagctagag
     7801 gtcgaccaat tctcatgttt gacagcttat catcgcagat ccgggcaacg ttgttgccat
     7861 tgctgcaggc gcagaactgg taggtatgga agatctatac attgaatcaa tattggcaat
     7921 tagccatatt agtcattggt tatatagcat aaatcaatat tggctattgg ccattgcata
     7981 cgttgtatct atatcataat atgtacattt atattggctc atgtccaata tgaccgc
//
