LOCUS       sequence-211199-       12872 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lenti-viral expression of Flag-tagged Depdc5 mutant C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12872)
  AUTHORS   Shen K, Huang RK, Brignole EJ, Condon KJ, Valenstein ML, 
            Chantranupong L, Bomaliyamu A, Choe A, Hong C, Yu Z, Sabatini DM
  TITLE     Architecture of the human GATOR1 and GATOR1-Rag GTPases complexes.
  JOURNAL   Nature. 2018 Apr 5;556(7699):64-69. doi: 10.1038/nature26158. Epub 
            2018 Mar 28.
  PUBMED    29590090
REFERENCE   2  (bases 1 to 12872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature26158"; journalName: "Nature"; date: "2018-04-5- 5"; 
            volume: "556"; issue: "7699"; pages: "64-69"
COMMENT     Sequence Label: [pSK479] pLJC5 Flag-Depdc5(C)
FEATURES             Location/Qualifiers
     source          1..12872
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     114..131
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    248..269
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(284..289,290..307,308..314)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    322..338
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     327..349
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     346..362
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     346..362
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     381..401
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        383..401
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        429..655
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             656..836
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    883..1008
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1501..1734
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1919..1963
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2252..3463
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3300..3319
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      3484..3493
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3493..3516
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    8394..8511
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        8560..9070
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8989..9007
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             9092..9691
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9092..9111)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9588..9608
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             9819..10052
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10124..10245
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10161..10180)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10215..10234
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10285..10420
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10440..10459)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10441..10459)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10469..10486)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10469..10485)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10478..10500)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10627..11082
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10714..10733)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10924..10945
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11108..11212
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11213..11281,11282..12073)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11431..11450)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12244..12832
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12733..12752
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
       61 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
      121 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
      181 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
      241 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
      301 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
      361 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
      421 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta
      481 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
      541 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac
      601 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc
      661 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
      721 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
      781 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
      841 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
      901 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
      961 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     1021 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     1081 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     1141 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     1201 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     1261 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     1321 aaaagtaaga ccaccgcaca gcaagcagca gctgatcttc agacctggag gaggagatat
     1381 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     1441 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     1501 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     1561 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     1621 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     1681 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     1741 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     1801 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     1861 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     1921 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     1981 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     2041 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     2101 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     2161 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
     2221 tcgatcacga gactagcctc gagcgggatc tggcctccgc gccgggtttt ggcgcctccc
     2281 gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag acgaagggcg cagcgagcgt
     2341 cctgatcctt ccgcccggac gctcaggaca gcggcccgct gctcataaga ctcggcctta
     2401 gaaccccagt atcagcagaa ggacatttta ggacgggact tgggtgactc tagggcactg
     2461 gttttctttc cagagagcgg aacaggcgag gaaaagtagt cccttctcgg cgattctgcg
     2521 gagggatctc cgtggggcgg tgaacgccga tgattatata aggacgcgcc gggtgtggca
     2581 cagctagttc cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga tcgctgtgat
     2641 cgtcacttgg tgagtagcgg gctgctgggc tggccggggc tttcgtggcc gccgggccgc
     2701 tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt ccgcgagcaa
     2761 ggttgccctg aactgggggt tggggggagc gcagcaaaat ggcggctgtt cccgagtctt
     2821 gaatggaaga cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg ggggcatggt
     2881 gggcggcaag aacccaaggt cttgaggcct tcgctaatgc gggaaagctc ttattcgggt
     2941 gagatgggct ggggcaccat ctggggaccc tgacgtgaag tttgtcactg actggagaac
     3001 tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt gccgttgggc agtgcacccg
     3061 tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt tggcttataa
     3121 tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct tttctccgtc gcaggacgca
     3181 gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg ccggacctct ggtgagggga
     3241 gggataagtg aggcgtcagt ttctttggtc ggttttatgt acctatcttc ttaagtagct
     3301 gaagctccgg ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg aagtttttta
     3361 ggcacctttt gaaatgtaat catttgggtc aatatgtaat tttcagtgtt agactagtaa
     3421 attgtccgct aaattctggc cgtttttggc ttttttgtta gacgaagcgc tagcgctacc
     3481 ggtgccacca tggactacaa ggatgacgat gacaagggtg gtggtggtgg tgcgtcgacg
     3541 atgagaacaa caaaggtcta caaactcgtc atccacaaga agggctttgg gggcagtgat
     3601 gatgagctag ttgtgaaccc caaagtgttc cctcacatca agcttggaga cattgtagag
     3661 attgcacacc ccaacgatga atacagccct ctgcttttgc aggtcaagtc tcttaaggaa
     3721 gatttacaga aggaaactat cagtgtggac cagactgtga ctcaagtgtt ccggctgaga
     3781 ccttatcagg atgtctatgt taatgtcgta gaccctaagg atgtgaccct tgacctagtg
     3841 gaattaactt ttaaggatca gtatattggc cgtggggata tgtggcgact aaagaaaagt
     3901 ttggtcagca catgtgccta tatcacccag aaggtggagt ttgctggcat cagagcacag
     3961 gctggtgaac tgtgggttaa gaatgagaag gtcatgtgtg gctacatcag tgaagatacc
     4021 agggtggtgt ttcgttctac gtcggctatg gtttacatat ttattcagat gagctgtgaa
     4081 atgtgggatt ttgatattta tggggatttg tattttgaga aagctgtgaa tggtttcctt
     4141 gctgatctat ttaccaagtg gaaggagaag aactgtagtc atgaagtgac agtggtcctg
     4201 ttttctagaa ctttctatga tgcaaaatct gttgatgaat ttcctgaaat aaaccgagcc
     4261 tcaattcgac aggatcataa ggggagattc tatgaagact tttacaaagt ggtggtgcag
     4321 aatgagagaa gagaagaatg gacttcactt ctcgtaacca ttaaaaaact cttcatccag
     4381 tatccagtgt tggtgcgact ggaacaggca gagggctttc ctcaaggaga taattctacc
     4441 tcagcacaag gaaactacct ggaggccatc aatctgtcat tcaatgtgtt tgataagcac
     4501 tacatcaacc gcaactttga ccgaactggg cagatgtcag tggtgatcac gcccggggtg
     4561 ggtgtctttg aagtggaccg cctactcatg atcctgacca agcagcggat gatagataat
     4621 ggaattggtg tggatttggt gtgcatggga gagcaaccgt tacatgctgt cccattgttc
     4681 aagctccata atcggagtgc tccccgtgat tctcgtctgg gcgatgacta taatatccct
     4741 cactggataa accacagttt ctacacatcc aaaagccagc tcttttgtaa tagtggcagc
     4801 ggaagcggca aactggcagg aaagaagccc gcctctgaga aagcaaaaaa tggccgtgat
     4861 acatctctcg ggagtccaaa agaatctgag aacgcccttc ccatccaagt agattatgac
     4921 gcctatgacg ctcaagtgtt caggctgccc ggcccatccc gggcccagtg cctcaccacc
     4981 tgcagatctg tgcgagagcg agagagtcac agtcgaaaga gtgccagctc ctgtgatgtt
     5041 tcatccagcc cttccctacc aagccgcaca ctgcccactg aggaagtgag gagccaggct
     5101 tctgacgaca gctccctagg caagagtgcc aacatcctga tgatcccaca cccccacctg
     5161 caccagtatg aagtcagcag ctccttggga tacaccagca ctcgagatgt cctggagaac
     5221 atgatggagc caccacagcg agactccagt gcaccaggga ggtttcacgt tggcagtgca
     5281 gaatccatgc tgcatgttcg acctggtgga tacacgcccc agagagcact gattaacccc
     5341 ttcgctccct ctcggatgcc catgaagctt acgtccaaca gaaggcgctg gatgcacact
     5401 tttcctgtgg ggccatccgg agaagccatc cagatccacc accagacccg acagaatatg
     5461 gcggagctac aaggcagcgg gcagagggat ccaactcact cctctgcaga gctgctggag
     5521 ttagcatatc atgaagctgc tggaaggcac agcaattccc gccagcctgg tgacggcatg
     5581 tccttcttga acttcagtgg aacagaggag ctttctgtcg gcctgcttag caacagtggt
     5641 gcaggtatga atcctaggac ccagaataag gattctctag aggacagtgt ttctacctct
     5701 ccagacccaa ttctgacact gtctgctccc cctgtagtgc caggcttctg ttgcacagtt
     5761 ggagtggact ggaagtctct cactactccg gcgtgcctcc cccttaccac cgactacttc
     5821 cctgaccgcc agggcctgca gaatgactac acagagggct gttatgatct ccttccagaa
     5881 gcagacatcg acaggaggga cgaagatggt gtgcagatga cagcccagca ggtatttgaa
     5941 gagtttattt gccaacgtct catgcagggc taccaaatca tagtgcagcc caagacacag
     6001 aaacccaatc ctgctgtccc gcccccgctg agcagtagcc cactctatag ccgaggcctt
     6061 gtgtcccgaa accgccctga ggaggaggac cagtattggc tgagtatggg cagaacgttc
     6121 cacaaagtga cgctgaagga taagatgatc acagtgacgc gataccttcc caagtatcct
     6181 tatgaatctg cccagatcca ctacacctac agcctctgtc cttcccactc agactcagag
     6241 ttcgtctcct gctgggtgga gttttcccac gaacggctgg aggagtacaa gtggaattac
     6301 ttagatcagt atatctgttc tgccggctct gaagacttca gcttaattga gtccctgaag
     6361 ttctggagga cccgcttcct gctgctgcca gcctgtgtca ccgccaccaa gcgcatcacg
     6421 gagggggagg cccactgcga catctatggg gacaggcccc gtgcagacga ggacgagtgg
     6481 caactcctgg atggttttgt ccgctttgtg gagggcttga atcgcattcg caggcggcat
     6541 cgctcggatc gcatgatgcg gaaagggacc gccatgaaag gcttgcagat gactgggccc
     6601 atttccacgc attctctgga gtcaactgca cccccagtgg ggaagaaggg aacctcagct
     6661 ctctctgccc tgttggagat ggaggccagt cagaagtgcc tgggagaaca gcaggcagct
     6721 gtgcatggtg ggaagagctc cgcccagtca gccgagagca gcagcgttgc catgactccc
     6781 acctacatgg acagcccacg aaaggacggg gccttcttta tggagtttgt ccgcagccca
     6841 cgcacagcat cgtccgcctt ctaccctcag gtatctgtgg accaaacagc cactcctatg
     6901 ttggacggca ccagtttggg catatgcaca ggccaatcca tggacagagg caacagccag
     6961 acctttggga actcccagaa cataggagaa cagggctact cctccacaaa ctccagtgac
     7021 agcagctctc agcagctggt ggcaagctcc ttgacctcat cctctaccct gacagagatc
     7081 ctggaagcca tgaagcaccc ctcgacagga gtccagctgc tctctgaaca gaagggcctc
     7141 tcaccgtact gcttcatcag cgcggaggtg gtacactggt tggtgaacca cgtggagggg
     7201 atccagacac aggcgatggc cattgacatc atgcagaaaa tgctggaaga gcagctcatc
     7261 acacatgcat ctggcgaagc ctggcggacc ttcatctacg gcttctattt ctacaagata
     7321 gtaacggaca aagagcccga ccgagtggcc atgcagcagc ccgccaccac ctggcacaca
     7381 gcaggagtgg acgacttcgc cagcttccag cgcaagtggt ttgaggtggc ctttgtggca
     7441 gaagagctcg tgcactctga gattcctgcc tttctcctgc cctggctgcc tagccggcca
     7501 gcctcctatg caagtaggca cagctccttt agccgaagtt ttggaggacg gagccaggcg
     7561 gcagcacttt tagctgccac tgtcccagag cagaggactg tgaccctgga tgttgacgtg
     7621 aacaaccgca cagaccggct ggagtggtgc agctgttatt accatggcaa cttttctctg
     7681 aatgcagcct ttgagatcaa gctgcactgg atggcggtga ccgcagcagt actcttcgag
     7741 atggtccaag gttggcatcg gaaagccacc tcctgtggct tcttgttagt cccagttttg
     7801 gaggggcctt ttgcactgcc cagttacctg tatggcgacc cccttcgtgc ccagctcttc
     7861 atcccactca acatcagctg cttgctcaag gagggcagcg agcacctgtt tgatagcttt
     7921 gaacccgaaa cgtactggga tcgaatgcac ctcttccagg aagccattgc acacaggttt
     7981 gggtttgtac aagataaata ttctgcctct gcttttaact tccctgctga gaacaagcct
     8041 cagtatatcc acgttacagg aacagtgttt ctgcagctgc cctactccaa gcgcaagttc
     8101 tcagggcagc agcggcggcg gcggaactcc accagctcca ccaaccagaa catgttctgc
     8161 gaggagcggg tcggctacaa ctgggcctac aacaccatgc tcaccaaaac atggcgctcc
     8221 agcgccacag gggatgaaaa gtttgctgat cggctgctga aggacttcac ggacttctgc
     8281 atcaaccgtg acaaccggct ggtcacgttc tggacaagtt gcctggagaa gatgcatgcc
     8341 agtgccccgt gagaattctc gacctcgaga caaatggcag tattcatcca caattttaaa
     8401 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     8461 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     8521 tacagggaca gcagagatcc actttggccg cggctcgagg gggttggggt tgcgcctttt
     8581 ccaaggcagc cctgggtttg cgcagggacg cggctgctct gggcgtggtt ccgggaaacg
     8641 cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat
     8701 cttcgccgct acccttgtgg gccccccggc gacgcttcct gctccgcccc taagtcggga
     8761 aggttccttg cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac gtctcactag
     8821 taccctcgca gacggacagc gccagggagc aatggcagcg cgccgaccgc gatgggctgt
     8881 ggccaatagc ggctgctcag cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg
     8941 ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt
     9001 ctgcaagcct ccggagcgca cgtcggcagt cggctccctc gttgaccgaa tcaccgacct
     9061 ctctccccag ggggatccac cggagcttac catgaccgag tacaagccca cggtgcgcct
     9121 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     9181 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     9241 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     9301 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     9361 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     9421 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     9481 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     9541 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     9601 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     9661 ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac
     9721 cgaaaggagc gcacgacccc atgcatcggt acctttaaga ccaatgactt acaaggcagc
     9781 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     9841 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     9901 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     9961 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
    10021 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
    10081 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
    10141 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
    10201 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
    10261 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
    10321 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
    10381 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc
    10441 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
    10501 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
    10561 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
    10621 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
    10681 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
    10741 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
    10801 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
    10861 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
    10921 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
    10981 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
    11041 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
    11101 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
    11161 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
    11221 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
    11281 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
    11341 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
    11401 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
    11461 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
    11521 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
    11581 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
    11641 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
    11701 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
    11761 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
    11821 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
    11881 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
    11941 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
    12001 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
    12061 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
    12121 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
    12181 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
    12241 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
    12301 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
    12361 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
    12421 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
    12481 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
    12541 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
    12601 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
    12661 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
    12721 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
    12781 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
    12841 caacgcggcc tttttacggt tcctggcctt tt
//
