LOCUS       sequence-21120-e         485 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG426GPD-Cerulean-ccdB
FEATURES             Location/Qualifiers
     source          1..485
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(264..280)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(264..280)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(369..392)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 aagtcctgct gcttcatgtg gtcggggtag cgggcgaagc actgcacgcc ccacgtcagg
       61 gtggtcacga gggtgggcca gggcacgggc agcttgccgg tggtgcagat gaacttcatg
      121 gtcagcttgc cgtaggtggc atcgccctcc ccctcgccgg acacgctgaa cttgtggccg
      181 tttacctcgc cgtccagctc gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc
      241 ttgctcacca ttttgtcccg ggggatccac tagttctaga atccgtcgaa actaagttct
      301 ggtgttttaa aactaaaaaa aagactaact ataaaagtag aatttaagaa gtttaagaaa
      361 tagatttaca gaattacaat caatacctac cgtctttata tacttattag tcaagtaggg
      421 gaataatttc agggaactgg tttcaacctt ttttttcagc tttttccaaa tcagagagag
      481 cagaa
//
