LOCUS       sequence-211202-       12884 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lenti-viral expression of HA-tagged Depdc5 mutant A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12884)
  AUTHORS   Shen K, Huang RK, Brignole EJ, Condon KJ, Valenstein ML, 
            Chantranupong L, Bomaliyamu A, Choe A, Hong C, Yu Z, Sabatini DM
  TITLE     Architecture of the human GATOR1 and GATOR1-Rag GTPases complexes.
  JOURNAL   Nature. 2018 Apr 5;556(7699):64-69. doi: 10.1038/nature26158. Epub 
            2018 Mar 28.
  PUBMED    29590090
REFERENCE   2  (bases 1 to 12884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature26158"; journalName: "Nature"; date: "2018-04-5- 5"; 
            volume: "556"; issue: "7699"; pages: "64-69"
COMMENT     Sequence Label: [pSK475] pLJC5 HA-Depdc5(A)
FEATURES             Location/Qualifiers
     source          1..12884
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    58..79
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(94..99,100..117,118..124)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    132..148
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     137..159
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     156..172
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     156..172
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     191..211
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        193..211
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        239..465
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             466..646
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    693..818
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1311..1544
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1729..1773
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2062..3273
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3110..3129
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      3294..3303
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3306..3332
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    8216..8333
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        8382..8892
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8811..8829
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8914..9513
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8914..8933)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9410..9430
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             9641..9874
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9946..10067
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9983..10002)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10037..10056
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10107..10242
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10262..10281)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10263..10281)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10291..10308)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10291..10307)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10300..10322)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10449..10904
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10536..10555)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10746..10767
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10930..11034
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11035..11103,11104..11895)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11253..11272)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12066..12654
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12555..12574
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12808..12825
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
       61 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
      121 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
      181 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
      241 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
      301 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
      361 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
      421 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
      481 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
      541 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
      601 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
      661 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
      721 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
      781 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
      841 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
      901 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
      961 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     1021 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     1081 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     1141 ccaccgcaca gcaagcagca gctgatcttc agacctggag gaggagatat gagggacaat
     1201 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     1261 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     1321 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     1381 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     1441 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     1501 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     1561 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     1621 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     1681 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     1741 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     1801 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     1861 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     1921 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     1981 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgatcacga
     2041 gactagcctc gagcgggatc tggcctccgc gccgggtttt ggcgcctccc gcgggcgccc
     2101 ccctcctcac ggcgagcgct gccacgtcag acgaagggcg cagcgagcgt cctgatcctt
     2161 ccgcccggac gctcaggaca gcggcccgct gctcataaga ctcggcctta gaaccccagt
     2221 atcagcagaa ggacatttta ggacgggact tgggtgactc tagggcactg gttttctttc
     2281 cagagagcgg aacaggcgag gaaaagtagt cccttctcgg cgattctgcg gagggatctc
     2341 cgtggggcgg tgaacgccga tgattatata aggacgcgcc gggtgtggca cagctagttc
     2401 cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg
     2461 tgagtagcgg gctgctgggc tggccggggc tttcgtggcc gccgggccgc tcggtgggac
     2521 ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt ccgcgagcaa ggttgccctg
     2581 aactgggggt tggggggagc gcagcaaaat ggcggctgtt cccgagtctt gaatggaaga
     2641 cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg ggggcatggt gggcggcaag
     2701 aacccaaggt cttgaggcct tcgctaatgc gggaaagctc ttattcgggt gagatgggct
     2761 ggggcaccat ctggggaccc tgacgtgaag tttgtcactg actggagaac tcggtttgtc
     2821 gtctgttgcg ggggcggcag ttatggcggt gccgttgggc agtgcacccg tacctttggg
     2881 agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt tggcttataa tgcagggtgg
     2941 ggccacctgc cggtaggtgt gcggtaggct tttctccgtc gcaggacgca gggttcgggc
     3001 ctagggtagg ctctcctgaa tcgacaggcg ccggacctct ggtgagggga gggataagtg
     3061 aggcgtcagt ttctttggtc ggttttatgt acctatcttc ttaagtagct gaagctccgg
     3121 ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg aagtttttta ggcacctttt
     3181 gaaatgtaat catttgggtc aatatgtaat tttcagtgtt agactagtaa attgtccgct
     3241 aaattctggc cgtttttggc ttttttgtta gacgaagcgc tagcgctacc ggtgccacca
     3301 tgggctaccc ctatgatgtg cctgactacg cagatctcaa tggtggtggt ggtgggtcga
     3361 cgatgagaac aacaaaggtc tacaaactcg tcatccacaa gaagggcttt gggggcagtg
     3421 atgatgagct agttgtgaac cccaaagtgt tccctcacat caagcttgga gacattgtag
     3481 agattgcaca ccccaacgat gaatacagcc ctctgctttt gcaggtcaag tctcttaagg
     3541 aagatttaca gaaggaaact atcagtgtgg accagactgt gactcaagtg ttccggctga
     3601 gaccttatca ggatgtctat gttaatgtcg tagaccctaa ggatgtgacc cttgacctag
     3661 tggaattaac ttttaaggat cagtatattg gccgtgggga tatgtggcga ctaaagaaaa
     3721 gtttggtcag cacatgtgcc tatatcaccc agaaggtgga gtttgctggc atcagagcac
     3781 aggctggtga actgtgggtt aagaatgaga aggtcatgtg tggctacatc agtgaagata
     3841 ccagggtggt gtttcgttct acgtcggcta tggtttacat atttattcag atgagctgtg
     3901 aaatgtggga ttttggcagc ggaagcggct tgtattttga gaaagctgtg aatggtttcc
     3961 ttgctgatct atttaccaag tggaaggaga agaactgtag tcatgaagtg acagtggtcc
     4021 tgttttctag aactttctat gatgcaaaat ctgttgatga atttcctgaa ataaaccgag
     4081 cctcaattcg acaggatcac aaggggagat tctatgaaga cttttacaaa gtggtggtgc
     4141 agaatgagag aagagaagaa tggacttcac ttctcgtaac cattaaaaaa ctcttcatcc
     4201 agtatccagt gttggtgcga ctggaacagg cagagggctt tcctcaagga gataattcta
     4261 cctcagcaca aggaaactac ctggaggcca tcaatctgtc attcaatgtg tttgataagc
     4321 actacatcaa ccgcaacttt gaccgaactg ggcagatgtc agtggtgatc acgcccgggg
     4381 tgggtgtctt tgaagtggac cgcctactca tgatcctgac caagcagcgg atgatagata
     4441 atggaattgg tgtggatttg gtgtgcatgg gagagcaacc gttacatgct gtcccattgt
     4501 tcaagctcca taatcggagt gctccccgtg attctcgtct gggcgatgac tataatatcc
     4561 ctcactggat aaaccacagt ttctacacat ccaaaagcca gctcttttgt aatagtttca
     4621 ccccacgaat aaaactggca ggaaagaagc ccgcctctga gaaagcaaaa aatggccgtg
     4681 atacatctct cgggagtcca aaagaatctg agaacgccct tcccatccaa gtagattatg
     4741 acgcctatga cgctcaagtg ttcaggctgc ccggcccatc ccgggcccag tgcctcacca
     4801 cctgcagatc tgtgcgagag cgagagagtc acagtcgaaa gagtgccagc tcctgtgatg
     4861 tttcatccag cccttcccta ccaagccgca cactgcccac tgaggaagtg aggagccagg
     4921 cttctgacga cagctcccta ggcaagagtg ccaacatcct gatgatccca cacccccacc
     4981 tgcaccagta tgaagtcagc agctccttgg gatacaccag cactcgagat gtcctggaga
     5041 acatgatgga gccaccacag cgagactcca gtgcaccagg gaggtttcac gttggcagtg
     5101 cagaatccat gctgcatgtt cgacctggtg gatacacgcc ccagagagca ctgattaacc
     5161 ccttcgctcc ctctcggatg cccatgaagc ttacgtccaa cagaaggcgc tggatgcaca
     5221 cttttcctgt ggggccatcc ggagaagcca tccagatcca ccaccagacc cgacagaata
     5281 tggcggagct acaaggcagc gggcagaggg atccaactca ctcctctgca gagctgctgg
     5341 agttagcata tcatgaagct gctggaaggc acagcaattc ccgccagcct ggtgacggca
     5401 tgtccttctt gaacttcagt ggaacagagg agctttctgt cggcctgctt agcaacagtg
     5461 gtgcaggtat gaatcctagg acccagaata aggattctct agaggacagt gtttctacct
     5521 ctccagaccc aattctgaca ctgtctgctc cccctgtagt gccaggcttc tgttgcacag
     5581 ttggagtgga ctggaagtct ctcactactc cggcgtgcct cccccttacc accgactact
     5641 tccctgaccg ccagggcctg cagaatgact acacagaggg ctgttatgat ctccttccag
     5701 aagcagacat cgacaggagg gacgaagatg gtgtgcagat gacagcccag caggtatttg
     5761 aagagtttat ttgccaacgt ctcatgcagg gctaccaaat catagtgcag cccaagacac
     5821 agaaacccaa tcctgctgtc ccgcccccgc tgagcagtag cccactctat agccgaggcc
     5881 ttgtgtcccg aaaccgccct gaggaggagg accagtattg gctgagtatg ggcagaacgt
     5941 tccacaaagt gacgctgaag gataagatga tcacagtgac gcgatacctt cccaagtatc
     6001 cttatgaatc tgcccagatc cactacacct acagcctctg tccttcccac tcagactcag
     6061 agttcgtctc ctgctgggtg gagttttccc acgaacggct ggaggagtac aagtggaatt
     6121 acttagatca gtatatctgt tctgccggct ctgaagactt cagcttaatt gagtccctga
     6181 agttctggag gacccgcttc ctgctgctgc cagcctgtgt caccgccacc aagcgcatca
     6241 cggaggggga ggcccactgc gacatctatg gggacaggcc ccgtgcagac gaggacgagt
     6301 ggcaactcct ggatggtttt gtccgctttg tggagggctt gaatcgcatt cgcaggcggc
     6361 atcgctcgga tcgcatgatg cggaaaggga ccgccatgaa aggcttgcag atgactgggc
     6421 ccatttccac gcattctctg gagtcaactg cacccccagt ggggaagaag ggaacctcag
     6481 ctctctctgc cctgttggag atggaggcca gtcagaagtg cctgggagaa cagcaggcag
     6541 ctgtgcatgg tgggaagagc tccgcccagt cagccgagag cagcagcgtt gccatgactc
     6601 ccacctacat ggacagccca cgaaaggacg gggccttctt tatggagttt gtccgcagcc
     6661 cacgcacagc atcgtccgcc ttctaccctc aggtatctgt ggaccaaaca gccactccta
     6721 tgttggacgg caccagtttg ggcatatgca caggccaatc catggacaga ggcaacagcc
     6781 agacctttgg gaactcccag aacataggag aacagggcta ctcctccaca aactccagtg
     6841 acagcagctc tcagcagctg gtggcaagct ccttgacctc atcctctacc ctgacagaga
     6901 tcctggaagc catgaagcac ccctcgacag gagtccagct gctctctgaa cagaagggcc
     6961 tctcaccgta ctgcttcatc agcgcggagg tggtacactg gttggtgaac cacgtggagg
     7021 ggatccagac acaggcgatg gccattgaca tcatgcagaa aatgctggaa gagcagctca
     7081 tcacacatgc atctggcgaa gcctggcgga ccttcatcta cggcttctat ttctacaaga
     7141 tagtaacgga caaagagccc gaccgagtgg ccatgcagca gcccgccacc acctggcaca
     7201 cagcaggagt ggacgacttc gccagcttcc agcgcaagtg gtttgaggtg gcctttgtgg
     7261 cagaagagct cgtgcactct gagattcctg cctttctcct gccctggctg cctagccggc
     7321 cagcctccta tgcaagtagg cacagctcct ttagccgaag ttttggagga cggagccagg
     7381 cggcagcact tttagctgcc actgtcccag agcagaggac tgtgaccctg gatgttgacg
     7441 tgaacaaccg cacagaccgg ctggagtggt gcagctgtta ttaccatggc aacttttctc
     7501 tgaatgcagc ctttgagatc aagctgcact ggatggcggt gaccgcagca gtactcttcg
     7561 agatggtcca aggttggcat cggaaagcca cctcctgtgg cttcttgtta gtcccagttt
     7621 tggaggggcc ttttgcactg cccagttacc tgtatggcga cccccttcgt gcccagctct
     7681 tcatcccact caacatcagc tgcttgctca aggagggcag cgagcacctg tttgatagct
     7741 ttgaacccga aacgtactgg gatcgaatgc acctcttcca ggaagccatt gcacacaggt
     7801 ttgggtttgt acaagataaa tattctgcct ctgcttttaa cttccctgct gagaacaagc
     7861 ctcagtatat ccacgttaca ggaacagtgt ttctgcagct gccctactcc aagcgcaagt
     7921 tctcagggca gcagcggcgg cggcggaact ccaccagctc caccaaccag aacatgttct
     7981 gcgaggagcg ggtcggctac aactgggcct acaacaccat gctcaccaaa acatggcgct
     8041 ccagcgccac aggggatgaa aagtttgctg atcggctgct gaaggacttc acggacttct
     8101 gcatcaaccg tgacaaccgg ctggtcacgt tctggacaag ttgcctggag aagatgcatg
     8161 ccagtgcccc gtgagaattc tcgacctcga gacaaatggc agtattcatc cacaatttta
     8221 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     8281 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     8341 attacaggga cagcagagat ccactttggc cgcggctcga gggggttggg gttgcgcctt
     8401 ttccaaggca gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa
     8461 cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg
     8521 atcttcgccg ctacccttgt gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg
     8581 gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc acgtctcact
     8641 agtaccctcg cagacggaca gcgccaggga gcaatggcag cgcgccgacc gcgatgggct
     8701 gtggccaata gcggctgctc agcagggcgc gccgagagca gcggccggga aggggcggtg
     8761 cgggaggcgg ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg gtgttccgca
     8821 ttctgcaagc ctccggagcg cacgtcggca gtcggctccc tcgttgaccg aatcaccgac
     8881 ctctctcccc agggggatcc accggagctt accatgaccg agtacaagcc cacggtgcgc
     8941 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
     9001 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
     9061 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
     9121 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
     9181 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
     9241 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
     9301 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
     9361 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
     9421 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
     9481 acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg
     9541 accgaaagga gcgcacgacc ccatgcatcg gtacctttaa gaccaatgac ttacaaggca
     9601 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     9661 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     9721 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     9781 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     9841 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     9901 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     9961 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
    10021 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
    10081 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
    10141 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
    10201 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc
    10261 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
    10321 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
    10381 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
    10441 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
    10501 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
    10561 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
    10621 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
    10681 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
    10741 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
    10801 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
    10861 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
    10921 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
    10981 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
    11041 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
    11101 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
    11161 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
    11221 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
    11281 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
    11341 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
    11401 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
    11461 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
    11521 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
    11581 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
    11641 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
    11701 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
    11761 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
    11821 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
    11881 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
    11941 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
    12001 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
    12061 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
    12121 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
    12181 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
    12241 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
    12301 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
    12361 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
    12421 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
    12481 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
    12541 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
    12601 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
    12661 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
    12721 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
    12781 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
    12841 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc atta
//
