LOCUS       sequence-211203-       12872 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lenti-viral expression of Flag-tagged wildtype Depdc5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12872)
  AUTHORS   Shen K, Huang RK, Brignole EJ, Condon KJ, Valenstein ML, 
            Chantranupong L, Bomaliyamu A, Choe A, Hong C, Yu Z, Sabatini DM
  TITLE     Architecture of the human GATOR1 and GATOR1-Rag GTPases complexes.
  JOURNAL   Nature. 2018 Apr 5;556(7699):64-69. doi: 10.1038/nature26158. Epub 
            2018 Mar 28.
  PUBMED    29590090
REFERENCE   2  (bases 1 to 12872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature26158"; journalName: "Nature"; date: "2018-04-5- 5"; 
            volume: "556"; issue: "7699"; pages: "64-69"
COMMENT     Sequence Label: [pSK468] pLJC5 Flag-Depdc5
FEATURES             Location/Qualifiers
     source          1..12872
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    128..149
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(164..169,170..187,188..194)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    202..218
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     207..229
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     226..242
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     226..242
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     261..281
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        263..281
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        309..535
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             536..716
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    763..888
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1381..1614
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1799..1843
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2132..3343
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3180..3199
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      3364..3373
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3373..3396
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    8274..8391
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        8440..8950
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8869..8887
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8972..9571
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8972..8991)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9468..9488
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             9699..9932
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10004..10125
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10041..10060)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10095..10114
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10165..10300
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10320..10339)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10321..10339)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10349..10366)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10349..10365)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10358..10380)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10507..10962
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10594..10613)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10804..10825
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10988..11092
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11093..11161,11162..11953)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11311..11330)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12124..12712
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12613..12632
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(12866..12872,1..11)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
       61 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
      121 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
      181 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
      241 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
      301 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta
      361 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
      421 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac
      481 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc
      541 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
      601 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
      661 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
      721 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
      781 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
      841 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
      901 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
      961 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     1021 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     1081 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     1141 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     1201 aaaagtaaga ccaccgcaca gcaagcagca gctgatcttc agacctggag gaggagatat
     1261 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     1321 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     1381 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     1441 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     1501 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     1561 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     1621 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     1681 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     1741 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     1801 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     1861 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     1921 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     1981 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     2041 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
     2101 tcgatcacga gactagcctc gagcgggatc tggcctccgc gccgggtttt ggcgcctccc
     2161 gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag acgaagggcg cagcgagcgt
     2221 cctgatcctt ccgcccggac gctcaggaca gcggcccgct gctcataaga ctcggcctta
     2281 gaaccccagt atcagcagaa ggacatttta ggacgggact tgggtgactc tagggcactg
     2341 gttttctttc cagagagcgg aacaggcgag gaaaagtagt cccttctcgg cgattctgcg
     2401 gagggatctc cgtggggcgg tgaacgccga tgattatata aggacgcgcc gggtgtggca
     2461 cagctagttc cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga tcgctgtgat
     2521 cgtcacttgg tgagtagcgg gctgctgggc tggccggggc tttcgtggcc gccgggccgc
     2581 tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt ccgcgagcaa
     2641 ggttgccctg aactgggggt tggggggagc gcagcaaaat ggcggctgtt cccgagtctt
     2701 gaatggaaga cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg ggggcatggt
     2761 gggcggcaag aacccaaggt cttgaggcct tcgctaatgc gggaaagctc ttattcgggt
     2821 gagatgggct ggggcaccat ctggggaccc tgacgtgaag tttgtcactg actggagaac
     2881 tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt gccgttgggc agtgcacccg
     2941 tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt tggcttataa
     3001 tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct tttctccgtc gcaggacgca
     3061 gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg ccggacctct ggtgagggga
     3121 gggataagtg aggcgtcagt ttctttggtc ggttttatgt acctatcttc ttaagtagct
     3181 gaagctccgg ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg aagtttttta
     3241 ggcacctttt gaaatgtaat catttgggtc aatatgtaat tttcagtgtt agactagtaa
     3301 attgtccgct aaattctggc cgtttttggc ttttttgtta gacgaagcgc tagcgctacc
     3361 ggtgccacca tggactacaa ggatgacgat gacaagggtg gtggtggtgg tgcgtcgacg
     3421 atgagaacaa caaaggtcta caaactcgtc atccacaaga agggctttgg gggcagtgat
     3481 gatgagctag ttgtgaaccc caaagtgttc cctcacatca agcttggaga cattgtagag
     3541 attgcacacc ccaacgatga atacagccct ctgcttttgc aggtcaagtc tcttaaggaa
     3601 gatttacaga aggaaactat cagtgtggac cagactgtga ctcaagtgtt ccggctgaga
     3661 ccttatcagg atgtctatgt taatgtcgta gaccctaagg atgtgaccct tgacctagtg
     3721 gaattaactt ttaaggatca gtatattggc cgtggggata tgtggcgact aaagaaaagt
     3781 ttggtcagca catgtgccta tatcacccag aaggtggagt ttgctggcat cagagcacag
     3841 gctggtgaac tgtgggttaa gaatgagaag gtcatgtgtg gctacatcag tgaagatacc
     3901 agggtggtgt ttcgttctac gtcggctatg gtttacatat ttattcagat gagctgtgaa
     3961 atgtgggatt ttgatattta tggggatttg tattttgaga aagctgtgaa tggtttcctt
     4021 gctgatctat ttaccaagtg gaaggagaag aactgtagtc atgaagtgac agtggtcctg
     4081 ttttctagaa ctttctatga tgcaaaatct gttgatgaat ttcctgaaat aaaccgagcc
     4141 tcaattcgac aggatcataa ggggagattc tatgaagact tttacaaagt ggtggtgcag
     4201 aatgagagaa gagaagaatg gacttcactt ctcgtaacca ttaaaaaact cttcatccag
     4261 tatccagtgt tggtgcgact ggaacaggca gagggctttc ctcaaggaga taattctacc
     4321 tcagcacaag gaaactacct ggaggccatc aatctgtcat tcaatgtgtt tgataagcac
     4381 tacatcaacc gcaactttga ccgaactggg cagatgtcag tggtgatcac gcccggggtg
     4441 ggtgtctttg aagtggaccg cctactcatg atcctgacca agcagcggat gatagataat
     4501 ggaattggtg tggatttggt gtgcatggga gagcaaccgt tacatgctgt cccattgttc
     4561 aagctccata atcggagtgc tccccgtgat tctcgtctgg gcgatgacta taatatccct
     4621 cactggataa accacagttt ctacacatcc aaaagccagc tcttttgtaa tagtttcacc
     4681 ccacgaataa aactggcagg aaagaagccc gcctctgaga aagcaaaaaa tggccgtgat
     4741 acatctctcg ggagtccaaa agaatctgag aacgcccttc ccatccaagt agattatgac
     4801 gcctatgacg ctcaagtgtt caggctgccc ggcccatccc gggcccagtg cctcaccacc
     4861 tgcagatctg tgcgagagcg agagagtcac agtcgaaaga gtgccagctc ctgtgatgtt
     4921 tcatccagcc cttccctacc aagccgcaca ctgcccactg aggaagtgag gagccaggct
     4981 tctgacgaca gctccctagg caagagtgcc aacatcctga tgatcccaca cccccacctg
     5041 caccagtatg aagtcagcag ctccttggga tacaccagca ctcgagatgt cctggagaac
     5101 atgatggagc caccacagcg agactccagt gcaccaggga ggtttcacgt cggcagtgca
     5161 gaatccatgc tgcatgttcg acctggtgga tacacgcccc agagagcact gattaacccc
     5221 ttcgctccct ctcggatgcc catgaagctt acgtccaaca gaaggcgctg gatgcacact
     5281 tttcctgtgg ggccatccgg agaagccatc cagatccacc accagacccg acagaatatg
     5341 gcggagctac aaggcagcgg gcagagggat ccaactcact cctctgcaga gctgctggag
     5401 ttagcatatc atgaagctgc tggaaggcac agcaattccc gccagcctgg tgacggcatg
     5461 tccttcttga acttcagtgg aacagaggag ctttctgtcg gcctgcttag caacagtggt
     5521 gcaggtatga atcctaggac ccagaataag gattctctag aggacagtgt ttctacctct
     5581 ccagacccaa ttctgacact gtctgctccc cctgtagtgc caggcttctg ttgcacagtt
     5641 ggagtggact ggaagtctct cactactccg gcgtgcctcc cccttaccac cgactacttc
     5701 cctgaccgcc agggcctgca gaatgactac acagagggct gttatgatct ccttccagaa
     5761 gcagacatcg acaggaggga cgaagatggt gtgcagatga cagcccagca ggtatttgaa
     5821 gagtttattt gccaacgtct catgcagggc taccaaatca tagtgcagcc caagacacag
     5881 aaacccaatc ctgctgtccc gcccccgctg agcagtagcc cactctatag ccgaggcctt
     5941 gtgtcccgaa accgccctga ggaggaggac cagtattggc tgagtatggg cagaacgttc
     6001 cacaaagtga cgctgaagga taagatgatc acagtgacgc gataccttcc caagtatcct
     6061 tatgaatctg cccagatcca ctacacctac agcctctgtc cttcccactc agactcagag
     6121 ttcgtctcct gctgggtgga gttttcccac gaacggctgg aggagtacaa gtggaattac
     6181 ttagatcagt atatctgttc tgccggctct gaagacttca gcttaattga gtccctgaag
     6241 ttctggagga cccgcttcct gctgctgcca gcctgtgtca ccgccaccaa gcgcatcacg
     6301 gagggggagg cccactgcga catctatggg gacaggcccc gtgcagacga ggacgagtgg
     6361 caactcctgg atggttttgt ccgctttgtg gagggcttga atcgcattcg caggcggcat
     6421 cgctcggatc gcatgatgcg gaaagggacc gccatgaaag gcttgcagat gactgggccc
     6481 atttccacgc attctctgga gtcaactgca cccccagtgg ggaagaaggg aacctcagct
     6541 ctctctgccc tgttggagat ggaggccagt cagaagtgcc tgggagaaca gcaggcagct
     6601 gtgcatggtg ggaagagctc cgcccagtca gccgagagca gcagcgttgc catgactccc
     6661 acctacatgg acagcccacg aaaggacggg gccttcttta tggagtttgt ccgcagccca
     6721 cgcacagcat cgtccgcctt ctaccctcag gtatctgtgg accaaacagc cactcctatg
     6781 ttggacggca ccagtttggg catatgcaca ggccaatcca tggacagagg caacagccag
     6841 acctttggga actcccagaa cataggagaa cagggctact cctccacaaa ctccagtgac
     6901 agcagctctc agcagctggt ggcaagctcc ttgacctcat cctctaccct gacagagatc
     6961 ctggaagcca tgaagcaccc ctcgacagga gtccagctgc tctctgaaca gaagggcctc
     7021 tcaccgtact gcttcatcag cgcggaggtg gtacactggt tggtgaacca cgtggagggg
     7081 atccagacac aggcgatggc cattgacatc atgcagaaaa tgctggaaga gcagctcatc
     7141 acacatgcat ctggcgaagc ctggcggacc ttcatctacg gcttctattt ctacaagata
     7201 gtaacggaca aagagcccga ccgagtggcc atgcagcagc ccgccaccac ctggcacaca
     7261 gcaggagtgg acgacttcgc cagcttccag cgcaagtggt ttgaggtggc ctttgtggca
     7321 gaagagctcg tgcactctga gattcctgcc tttctcctgc cctggctgcc tagccggcca
     7381 gcctcctatg caagtaggca cagctccttt agccgaagtt ttggaggacg gagccaggcg
     7441 gcagcacttt tagctgccac tgtcccagag cagaggactg tgaccctgga tgttgacgtg
     7501 aacaaccgca cagaccggct ggagtggtgc agctgttatt accatggcaa cttttctctg
     7561 aatgcagcct ttgagatcaa gctgcactgg atggcggtga ccgcagcagt actcttcgag
     7621 atggtccaag gttggcatcg gaaagccacc tcctgtggct tcttgttagt cccagttttg
     7681 gaggggcctt ttgcactgcc cagttacctg tatggcgacc cccttcgtgc ccagctcttc
     7741 atcccactca acatcagctg cttgctcaag gagggcagcg agcacctgtt tgatagcttt
     7801 gaacccgaaa cgtactggga tcgaatgcac ctcttccagg aagccattgc acacaggttt
     7861 gggtttgtac aagataaata ttctgcctct gcttttaact tccctgctga gaacaagcct
     7921 cagtatatcc acgttacagg aacagtgttt ctgcagctgc cctactccaa gcgcaagttc
     7981 tcagggcagc agcggcggcg gcggaactcc accagctcca ccaaccagaa catgttctgc
     8041 gaggagcggg tcggctacaa ctgggcctac aacaccatgc tcaccaaaac atggcgctcc
     8101 agcgccacag gggatgaaaa gtttgctgat cggctgctga aggacttcac ggacttctgc
     8161 atcaaccgtg acaaccggct ggtcacgttc tggacaagtt gcctggagaa gatgcatgcc
     8221 agtgccccgt gagaattctc gacctcgaga caaatggcag tattcatcca caattttaaa
     8281 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     8341 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     8401 tacagggaca gcagagatcc actttggccg cggctcgagg gggttggggt tgcgcctttt
     8461 ccaaggcagc cctgggtttg cgcagggacg cggctgctct gggcgtggtt ccgggaaacg
     8521 cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat
     8581 cttcgccgct acccttgtgg gccccccggc gacgcttcct gctccgcccc taagtcggga
     8641 aggttccttg cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac gtctcactag
     8701 taccctcgca gacggacagc gccagggagc aatggcagcg cgccgaccgc gatgggctgt
     8761 ggccaatagc ggctgctcag cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg
     8821 ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt
     8881 ctgcaagcct ccggagcgca cgtcggcagt cggctccctc gttgaccgaa tcaccgacct
     8941 ctctccccag ggggatccac cggagcttac catgaccgag tacaagccca cggtgcgcct
     9001 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     9061 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     9121 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     9181 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     9241 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     9301 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     9361 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     9421 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     9481 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     9541 ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac
     9601 cgaaaggagc gcacgacccc atgcatcggt acctttaaga ccaatgactt acaaggcagc
     9661 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     9721 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     9781 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     9841 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     9901 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     9961 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
    10021 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
    10081 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
    10141 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
    10201 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
    10261 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc
    10321 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
    10381 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
    10441 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
    10501 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
    10561 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
    10621 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
    10681 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
    10741 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
    10801 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
    10861 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
    10921 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
    10981 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
    11041 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
    11101 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
    11161 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
    11221 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
    11281 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
    11341 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
    11401 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
    11461 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
    11521 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
    11581 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
    11641 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
    11701 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
    11761 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
    11821 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
    11881 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
    11941 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
    12001 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
    12061 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
    12121 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
    12181 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
    12241 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
    12301 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
    12361 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
    12421 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
    12481 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
    12541 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
    12601 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
    12661 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
    12721 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
    12781 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
    12841 tcgccgcagc cgaacgaccg agcgcagcga gt
//
