LOCUS       sequence-211205-       12872 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lenti-viral expression of Flag-tagged Depdc5 mutant A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12872)
  AUTHORS   Shen K, Huang RK, Brignole EJ, Condon KJ, Valenstein ML, 
            Chantranupong L, Bomaliyamu A, Choe A, Hong C, Yu Z, Sabatini DM
  TITLE     Architecture of the human GATOR1 and GATOR1-Rag GTPases complexes.
  JOURNAL   Nature. 2018 Apr 5;556(7699):64-69. doi: 10.1038/nature26158. Epub 
            2018 Mar 28.
  PUBMED    29590090
REFERENCE   2  (bases 1 to 12872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature26158"; journalName: "Nature"; date: "2018-04-5- 5"; 
            volume: "556"; issue: "7699"; pages: "64-69"
COMMENT     Sequence Label: [pSK480] pLJC5 Flag-Depdc5(A)
FEATURES             Location/Qualifiers
     source          1..12872
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    121..142
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(157..162,163..180,181..187)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    195..211
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     200..222
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     219..235
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     219..235
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     254..274
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        256..274
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        302..528
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             529..709
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    756..881
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1374..1607
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1792..1836
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2125..3336
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3173..3192
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      3357..3366
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3366..3389
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    8267..8384
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        8433..8943
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8862..8880
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8965..9564
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8965..8984)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9461..9481
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             9692..9925
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9997..10118
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10034..10053)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10088..10107
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10158..10293
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10313..10332)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10314..10332)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10342..10359)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10342..10358)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10351..10373)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10500..10955
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10587..10606)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10797..10818
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10981..11085
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11086..11154,11155..11946)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11304..11323)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12117..12705
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12606..12625
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(12859..12872,1..4)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
       61 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
      121 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
      181 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      241 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
      301 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
      361 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
      421 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
      481 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      541 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      601 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      661 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      721 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      781 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      841 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      901 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      961 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     1021 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1081 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1141 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1201 agaccaccgc acagcaagca gcagctgatc ttcagacctg gaggaggaga tatgagggac
     1261 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1321 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1381 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1441 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1501 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1561 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1621 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1681 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     1741 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     1801 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     1861 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     1921 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     1981 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     2041 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgatca
     2101 cgagactagc ctcgagcggg atctggcctc cgcgccgggt tttggcgcct cccgcgggcg
     2161 cccccctcct cacggcgagc gctgccacgt cagacgaagg gcgcagcgag cgtcctgatc
     2221 cttccgcccg gacgctcagg acagcggccc gctgctcata agactcggcc ttagaacccc
     2281 agtatcagca gaaggacatt ttaggacggg acttgggtga ctctagggca ctggttttct
     2341 ttccagagag cggaacaggc gaggaaaagt agtcccttct cggcgattct gcggagggat
     2401 ctccgtgggg cggtgaacgc cgatgattat ataaggacgc gccgggtgtg gcacagctag
     2461 ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt ggatcgctgt gatcgtcact
     2521 tggtgagtag cgggctgctg ggctggccgg ggctttcgtg gccgccgggc cgctcggtgg
     2581 gacggaagcg tgtggagaga ccgccaaggg ctgtagtctg ggtccgcgag caaggttgcc
     2641 ctgaactggg ggttgggggg agcgcagcaa aatggcggct gttcccgagt cttgaatgga
     2701 agacgcttgt gaggcgggct gtgaggtcgt tgaaacaagg tggggggcat ggtgggcggc
     2761 aagaacccaa ggtcttgagg ccttcgctaa tgcgggaaag ctcttattcg ggtgagatgg
     2821 gctggggcac catctgggga ccctgacgtg aagtttgtca ctgactggag aactcggttt
     2881 gtcgtctgtt gcgggggcgg cagttatggc ggtgccgttg ggcagtgcac ccgtaccttt
     2941 gggagcgcgc gccctcgtcg tgtcgtgacg tcacccgttc tgttggctta taatgcaggg
     3001 tggggccacc tgccggtagg tgtgcggtag gcttttctcc gtcgcaggac gcagggttcg
     3061 ggcctagggt aggctctcct gaatcgacag gcgccggacc tctggtgagg ggagggataa
     3121 gtgaggcgtc agtttctttg gtcggtttta tgtacctatc ttcttaagta gctgaagctc
     3181 cggttttgaa ctatgcgctc ggggttggcg agtgtgtttt gtgaagtttt ttaggcacct
     3241 tttgaaatgt aatcatttgg gtcaatatgt aattttcagt gttagactag taaattgtcc
     3301 gctaaattct ggccgttttt ggcttttttg ttagacgaag cgctagcgct accggtgcca
     3361 ccatggacta caaggatgac gatgacaagg gtggtggtgg tggtgcgtcg acgatgagaa
     3421 caacaaaggt ctacaaactc gtcatccaca agaagggctt tgggggcagt gatgatgagc
     3481 tagttgtgaa ccccaaagtg ttccctcaca tcaagcttgg agacattgta gagattgcac
     3541 accccaacga tgaatacagc cctctgcttt tgcaggtcaa gtctcttaag gaagatttac
     3601 agaaggaaac tatcagtgtg gaccagactg tgactcaagt gttccggctg agaccttatc
     3661 aggatgtcta tgttaatgtc gtagacccta aggatgtgac ccttgaccta gtggaattaa
     3721 cttttaagga tcagtatatt ggccgtgggg atatgtggcg actaaagaaa agtttggtca
     3781 gcacatgtgc ctatatcacc cagaaggtgg agtttgctgg catcagagca caggctggtg
     3841 aactgtgggt taagaatgag aaggtcatgt gtggctacat cagtgaagat accagggtgg
     3901 tgtttcgttc tacgtcggct atggtttaca tatttattca gatgagctgt gaaatgtggg
     3961 attttggcag cggaagcggc ttgtattttg agaaagctgt gaatggtttc cttgctgatc
     4021 tatttaccaa gtggaaggag aagaactgta gtcatgaagt gacagtggtc ctgttttcta
     4081 gaactttcta tgatgcaaaa tctgttgatg aatttcctga aataaaccga gcctcaattc
     4141 gacaggatca caaggggaga ttctatgaag acttttacaa agtggtggtg cagaatgaga
     4201 gaagagaaga atggacttca cttctcgtaa ccattaaaaa actcttcatc cagtatccag
     4261 tgttggtgcg actggaacag gcagagggct ttcctcaagg agataattct acctcagcac
     4321 aaggaaacta cctggaggcc atcaatctgt cattcaatgt gtttgataag cactacatca
     4381 accgcaactt tgaccgaact gggcagatgt cagtggtgat cacgcccggg gtgggtgtct
     4441 ttgaagtgga ccgcctactc atgatcctga ccaagcagcg gatgatagat aatggaattg
     4501 gtgtggattt ggtgtgcatg ggagagcaac cgttacatgc tgtcccattg ttcaagctcc
     4561 ataatcggag tgctccccgt gattctcgtc tgggcgatga ctataatatc cctcactgga
     4621 taaaccacag tttctacaca tccaaaagcc agctcttttg taatagtttc accccacgaa
     4681 taaaactggc aggaaagaag cccgcctctg agaaagcaaa aaatggccgt gatacatctc
     4741 tcgggagtcc aaaagaatct gagaacgccc ttcccatcca agtagattat gacgcctatg
     4801 acgctcaagt gttcaggctg cccggcccat cccgggccca gtgcctcacc acctgcagat
     4861 ctgtgcgaga gcgagagagt cacagtcgaa agagtgccag ctcctgtgat gtttcatcca
     4921 gcccttccct accaagccgc acactgccca ctgaggaagt gaggagccag gcttctgacg
     4981 acagctccct aggcaagagt gccaacatcc tgatgatccc acacccccac ctgcaccagt
     5041 atgaagtcag cagctccttg ggatacacca gcactcgaga tgtcctggag aacatgatgg
     5101 agccaccaca gcgagactcc agtgcaccag ggaggtttca cgttggcagt gcagaatcca
     5161 tgctgcatgt tcgacctggt ggatacacgc cccagagagc actgattaac cccttcgctc
     5221 cctctcggat gcccatgaag cttacgtcca acagaaggcg ctggatgcac acttttcctg
     5281 tggggccatc cggagaagcc atccagatcc accaccagac ccgacagaat atggcggagc
     5341 tacaaggcag cgggcagagg gatccaactc actcctctgc agagctgctg gagttagcat
     5401 atcatgaagc tgctggaagg cacagcaatt cccgccagcc tggtgacggc atgtccttct
     5461 tgaacttcag tggaacagag gagctttctg tcggcctgct tagcaacagt ggtgcaggta
     5521 tgaatcctag gacccagaat aaggattctc tagaggacag tgtttctacc tctccagacc
     5581 caattctgac actgtctgct ccccctgtag tgccaggctt ctgttgcaca gttggagtgg
     5641 actggaagtc tctcactact ccggcgtgcc tcccccttac caccgactac ttccctgacc
     5701 gccagggcct gcagaatgac tacacagagg gctgttatga tctccttcca gaagcagaca
     5761 tcgacaggag ggacgaagat ggtgtgcaga tgacagccca gcaggtattt gaagagttta
     5821 tttgccaacg tctcatgcag ggctaccaaa tcatagtgca gcccaagaca cagaaaccca
     5881 atcctgctgt cccgcccccg ctgagcagta gcccactcta tagccgaggc cttgtgtccc
     5941 gaaaccgccc tgaggaggag gaccagtatt ggctgagtat gggcagaacg ttccacaaag
     6001 tgacgctgaa ggataagatg atcacagtga cgcgatacct tcccaagtat ccttatgaat
     6061 ctgcccagat ccactacacc tacagcctct gtccttccca ctcagactca gagttcgtct
     6121 cctgctgggt ggagttttcc cacgaacggc tggaggagta caagtggaat tacttagatc
     6181 agtatatctg ttctgccggc tctgaagact tcagcttaat tgagtccctg aagttctgga
     6241 ggacccgctt cctgctgctg ccagcctgtg tcaccgccac caagcgcatc acggaggggg
     6301 aggcccactg cgacatctat ggggacaggc cccgtgcaga cgaggacgag tggcaactcc
     6361 tggatggttt tgtccgcttt gtggagggct tgaatcgcat tcgcaggcgg catcgctcgg
     6421 atcgcatgat gcggaaaggg accgccatga aaggcttgca gatgactggg cccatttcca
     6481 cgcattctct ggagtcaact gcacccccag tggggaagaa gggaacctca gctctctctg
     6541 ccctgttgga gatggaggcc agtcagaagt gcctgggaga acagcaggca gctgtgcatg
     6601 gtgggaagag ctccgcccag tcagccgaga gcagcagcgt tgccatgact cccacctaca
     6661 tggacagccc acgaaaggac ggggccttct ttatggagtt tgtccgcagc ccacgcacag
     6721 catcgtccgc cttctaccct caggtatctg tggaccaaac agccactcct atgttggacg
     6781 gcaccagttt gggcatatgc acaggccaat ccatggacag aggcaacagc cagacctttg
     6841 ggaactccca gaacatagga gaacagggct actcctccac aaactccagt gacagcagct
     6901 ctcagcagct ggtggcaagc tccttgacct catcctctac cctgacagag atcctggaag
     6961 ccatgaagca cccctcgaca ggagtccagc tgctctctga acagaagggc ctctcaccgt
     7021 actgcttcat cagcgcggag gtggtacact ggttggtgaa ccacgtggag gggatccaga
     7081 cacaggcgat ggccattgac atcatgcaga aaatgctgga agagcagctc atcacacatg
     7141 catctggcga agcctggcgg accttcatct acggcttcta tttctacaag atagtaacgg
     7201 acaaagagcc cgaccgagtg gccatgcagc agcccgccac cacctggcac acagcaggag
     7261 tggacgactt cgccagcttc cagcgcaagt ggtttgaggt ggcctttgtg gcagaagagc
     7321 tcgtgcactc tgagattcct gcctttctcc tgccctggct gcctagccgg ccagcctcct
     7381 atgcaagtag gcacagctcc tttagccgaa gttttggagg acggagccag gcggcagcac
     7441 ttttagctgc cactgtccca gagcagagga ctgtgaccct ggatgttgac gtgaacaacc
     7501 gcacagaccg gctggagtgg tgcagctgtt attaccatgg caacttttct ctgaatgcag
     7561 cctttgagat caagctgcac tggatggcgg tgaccgcagc agtactcttc gagatggtcc
     7621 aaggttggca tcggaaagcc acctcctgtg gcttcttgtt agtcccagtt ttggaggggc
     7681 cttttgcact gcccagttac ctgtatggcg acccccttcg tgcccagctc ttcatcccac
     7741 tcaacatcag ctgcttgctc aaggagggca gcgagcacct gtttgatagc tttgaacccg
     7801 aaacgtactg ggatcgaatg cacctcttcc aggaagccat tgcacacagg tttgggtttg
     7861 tacaagataa atattctgcc tctgctttta acttccctgc tgagaacaag cctcagtata
     7921 tccacgttac aggaacagtg tttctgcagc tgccctactc caagcgcaag ttctcagggc
     7981 agcagcggcg gcggcggaac tccaccagct ccaccaacca gaacatgttc tgcgaggagc
     8041 gggtcggcta caactgggcc tacaacacca tgctcaccaa aacatggcgc tccagcgcca
     8101 caggggatga aaagtttgct gatcggctgc tgaaggactt cacggacttc tgcatcaacc
     8161 gtgacaaccg gctggtcacg ttctggacaa gttgcctgga gaagatgcat gccagtgccc
     8221 cgtgagaatt ctcgacctcg agacaaatgg cagtattcat ccacaatttt aaaagaaaag
     8281 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     8341 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     8401 acagcagaga tccactttgg ccgcggctcg agggggttgg ggttgcgcct tttccaaggc
     8461 agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc
     8521 gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc
     8581 gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc
     8641 ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc
     8701 gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat
     8761 agcggctgct cagcagggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg
     8821 gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag
     8881 cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc
     8941 cagggggatc caccggagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc
     9001 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     9061 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     9121 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     9181 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     9241 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     9301 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     9361 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     9421 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     9481 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     9541 atgacccgca agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg
     9601 agcgcacgac cccatgcatc ggtaccttta agaccaatga cttacaaggc agctgtagat
     9661 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     9721 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     9781 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     9841 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     9901 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     9961 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
    10021 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
    10081 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
    10141 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
    10201 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
    10261 tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag
    10321 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
    10381 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
    10441 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga
    10501 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
    10561 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
    10621 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
    10681 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
    10741 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
    10801 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
    10861 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
    10921 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
    10981 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
    11041 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
    11101 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
    11161 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
    11221 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
    11281 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
    11341 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
    11401 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
    11461 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
    11521 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
    11581 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
    11641 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
    11701 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
    11761 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
    11821 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
    11881 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
    11941 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
    12001 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
    12061 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
    12121 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
    12181 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
    12241 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
    12301 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
    12361 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
    12421 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
    12481 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
    12541 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
    12601 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
    12661 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
    12721 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
    12781 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
    12841 agccgaacga ccgagcgcag cgagtcagtg ag
//
