LOCUS       sequence-211209-       12872 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lenti-viral expression of Flag-tagged Depdc5 mutant P.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12872)
  AUTHORS   Shen K, Huang RK, Brignole EJ, Condon KJ, Valenstein ML, 
            Chantranupong L, Bomaliyamu A, Choe A, Hong C, Yu Z, Sabatini DM
  TITLE     Architecture of the human GATOR1 and GATOR1-Rag GTPases complexes.
  JOURNAL   Nature. 2018 Apr 5;556(7699):64-69. doi: 10.1038/nature26158. Epub 
            2018 Mar 28.
  PUBMED    29590090
REFERENCE   2  (bases 1 to 12872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature26158"; journalName: "Nature"; date: "2018-04-5- 5"; 
            volume: "556"; issue: "7699"; pages: "64-69"
COMMENT     Sequence Label: [pSK483] pLJC5 Flag-Depdc5(P)
FEATURES             Location/Qualifiers
     source          1..12872
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    79..100
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(115..120,121..138,139..145)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    153..169
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     158..180
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     177..193
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     177..193
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     212..232
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        214..232
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        260..486
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             487..667
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    714..839
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1332..1565
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1750..1794
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2083..3294
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3131..3150
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      3315..3324
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3324..3347
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    8225..8342
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        8391..8901
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8820..8838
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8923..9522
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8923..8942)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9419..9439
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             9650..9883
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9955..10076
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9992..10011)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10046..10065
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10116..10251
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10271..10290)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10272..10290)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10300..10317)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10300..10316)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10309..10331)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10458..10913
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10545..10564)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10755..10776
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10939..11043
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11044..11112,11113..11904)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11262..11281)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12075..12663
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12564..12583
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12817..12834
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
       61 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
      121 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
      181 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa
      241 gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta
      301 acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg
      361 gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt
      421 ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac
      481 ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
      541 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
      601 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
      661 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
      721 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
      781 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
      841 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
      901 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
      961 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
     1021 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
     1081 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
     1141 aagagcaaaa caaaagtaag accaccgcac agcaagcagc agctgatctt cagacctgga
     1201 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
     1261 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
     1321 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
     1381 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
     1441 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
     1501 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
     1561 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
     1621 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
     1681 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
     1741 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
     1801 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
     1861 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
     1921 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa
     1981 ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga
     2041 tctcgacggt atcgatcacg agactagcct cgagcgggat ctggcctccg cgccgggttt
     2101 tggcgcctcc cgcgggcgcc cccctcctca cggcgagcgc tgccacgtca gacgaagggc
     2161 gcagcgagcg tcctgatcct tccgcccgga cgctcaggac agcggcccgc tgctcataag
     2221 actcggcctt agaaccccag tatcagcaga aggacatttt aggacgggac ttgggtgact
     2281 ctagggcact ggttttcttt ccagagagcg gaacaggcga ggaaaagtag tcccttctcg
     2341 gcgattctgc ggagggatct ccgtggggcg gtgaacgccg atgattatat aaggacgcgc
     2401 cgggtgtggc acagctagtt ccgtcgcagc cgggatttgg gtcgcggttc ttgtttgtgg
     2461 atcgctgtga tcgtcacttg gtgagtagcg ggctgctggg ctggccgggg ctttcgtggc
     2521 cgccgggccg ctcggtggga cggaagcgtg tggagagacc gccaagggct gtagtctggg
     2581 tccgcgagca aggttgccct gaactggggg ttggggggag cgcagcaaaa tggcggctgt
     2641 tcccgagtct tgaatggaag acgcttgtga ggcgggctgt gaggtcgttg aaacaaggtg
     2701 gggggcatgg tgggcggcaa gaacccaagg tcttgaggcc ttcgctaatg cgggaaagct
     2761 cttattcggg tgagatgggc tggggcacca tctggggacc ctgacgtgaa gtttgtcact
     2821 gactggagaa ctcggtttgt cgtctgttgc gggggcggca gttatggcgg tgccgttggg
     2881 cagtgcaccc gtacctttgg gagcgcgcgc cctcgtcgtg tcgtgacgtc acccgttctg
     2941 ttggcttata atgcagggtg gggccacctg ccggtaggtg tgcggtaggc ttttctccgt
     3001 cgcaggacgc agggttcggg cctagggtag gctctcctga atcgacaggc gccggacctc
     3061 tggtgagggg agggataagt gaggcgtcag tttctttggt cggttttatg tacctatctt
     3121 cttaagtagc tgaagctccg gttttgaact atgcgctcgg ggttggcgag tgtgttttgt
     3181 gaagtttttt aggcaccttt tgaaatgtaa tcatttgggt caatatgtaa ttttcagtgt
     3241 tagactagta aattgtccgc taaattctgg ccgtttttgg cttttttgtt agacgaagcg
     3301 ctagcgctac cggtgccacc atggactaca aggatgacga tgacaagggt ggtggtggtg
     3361 gtgcgtcgac gatgagaaca acaaaggtct acaaactcgt catccacaag aagggctttg
     3421 ggggcagtga tgatgagcta gttgtgaacc ccaaagtgtt ccctcacatc aagcttggag
     3481 acattgtaga gattgcacac cccaacgatg aatacagccc tctgcttttg caggtcaagt
     3541 ctcttaagga agatttacag aaggaaacta tcagtgtgga ccagactgtg actcaagtgt
     3601 tccggctgag accttatcag gatgtctatg ttaatgtcgt agaccctaag gatgtgaccc
     3661 ttgacctagt ggaattaact tttaaggatc agtatattgg ccgtggggat atgtggcgac
     3721 taaagaaaag tttggtcagc acatgtgcct atatcaccca gaaggtggag tttgctggca
     3781 tcagagcaca ggctggtgaa ctgtgggtta agaatgagaa ggtcatgtgt ggctacatca
     3841 gtgaagatac cagggtggtg tttcgttcta cgtcggctat ggtttacata tttattcaga
     3901 tgagctgtga aatgtgggat tttgatattt atggggattt gtattttgag aaagctgtga
     3961 atggtttcct tgctgatcta tttaccaagt ggaaggagaa gaactgtagt catgaagtga
     4021 cagtggtcct gttttctaga actttctatg atgcaaaatc tgttgatgaa tttcctgaaa
     4081 taaaccgagc ctcaattcga caggatcata aggggagatt ctatgaagac ttttacaaag
     4141 tggtggtgca gaatgagaga agagaagaat ggacttcact tctcgtaacc attaaaaaac
     4201 tcttcatcca gtatccagtg ttggtgcgac tggaacaggc agagggcttt cctcaaggag
     4261 ataattctac ctcagcacaa ggaaactacc tggaggccat caatctgtca ttcaatgtgt
     4321 ttgataagca ctacatcaac cgcaactttg accgaactgg gcagatgtca gtggtgatca
     4381 cgcccggggt gggtgtcttt gaagtggacc gcctactcat gatcctgacc aagcagcgga
     4441 tgatagataa tggaattggt gtggatttgg tgtgcatggg agagcaaccg ttacatgctg
     4501 tcccattgtt caagctccat aatcggagtg ctccccgtga ttctcgtctg ggcgatgact
     4561 ataatatccc tcactggata aaccacagtt tctacacatc caaaagccag ctcttttgta
     4621 atagtttcac cccacgaata aaactggcag gaaagaagcc cgcctctgag aaagcaaaaa
     4681 atggccgtga tacatctctc gggagtccaa aagaatctga gaacgccctt cccatccaag
     4741 tagattatga cgcctatgac gctcaagtgt tcaggctgcc cggcccatcc cgggcccagt
     4801 gcctcaccac ctgcagatct gtgcgagagc gagagagtca cagtcgaaag agtgccagct
     4861 cctgtgatgt ttcatccagc ccttccctac caagccgcac actgcccact gaggaagtga
     4921 ggagccaggc ttctgacgac agctccctag gcaagagtgc caacatcctg atgatcccac
     4981 acccccacct gcaccagtat gaagtcagca gctccttggg atacaccagc actcgagatg
     5041 tcctggagaa catgatggag ccaccacagc gagactccag tgcaccaggg aggtttcacg
     5101 tcggcagtgc agaatccatg ctgcatgttc gacctggtgg atacacgccc cagagagcac
     5161 tgattaaccc cttcgctccc tctcggatgc ccatgaagct tacgtccaac agaaggcgct
     5221 ggatgcacac ttttcctgtg gggccatccg gagaagccat ccagatccac caccagaccc
     5281 gacagaatat ggcggagcta caaggcagcg ggcagaggga tccaactcac tcctctgcag
     5341 agctgctgga gttagcatat catgaagctg ctggaaggca cagcaattcc cgccagcctg
     5401 gtgacggcat gtccttcttg aacttcagtg gaacagagga gctttctgtc ggcctgctta
     5461 gcaacagtgg tgcaggtatg aatcctagga cccagaataa ggattctcta gaggacagtg
     5521 tttctacctc tccagaccca attctgacac tgtctgctcc ccctgtagtg ccaggcttct
     5581 gttgcacagt tggagtggac tggaagtctc tcactactcc ggcgtgcctc ccccttacca
     5641 ccgactactt ccctgaccgc cagggcctgc agaatgacta cacagagggc tgtggcagcg
     5701 gaagcggcga agcagacatc gacaggaggg acgaagatgg tgtgcagatg acagcccagc
     5761 aggtatttga agagtttatt tgccaacgtc tcatgcaggg ctaccaaatc atagtgcagc
     5821 ccaagacaca gaaacccaat cctgctgtcc cgcccccgct gagcagtagc ccactctata
     5881 gccgaggcct tgtgtcccga aaccgccctg aggaggagga ccagtattgg ctgagtatgg
     5941 gcagaacgtt ccacaaagtg acgctgaagg ataagatgat cacagtgacg cgataccttc
     6001 ccaagtatcc ttatgaatct gcccagatcc actacaccta cagcctctgt ccttcccact
     6061 cagactcaga gttcgtctcc tgctgggtgg agttttccca cgaacggctg gaggagtaca
     6121 agtggaatta cttagatcag tatatctgtt ctgccggctc tgaagacttc agcttaattg
     6181 agtccctgaa gttctggagg acccgcttcc tgctgctgcc agcctgtgtc accgccacca
     6241 agcgcatcac ggagggggag gcccactgcg acatctatgg ggacaggccc cgtgcagacg
     6301 aggacgagtg gcaactcctg gatggttttg tccgctttgt ggagggcttg aatcgcattc
     6361 gcaggcggca tcgctcggat cgcatgatgc ggaaagggac cgccatgaaa ggcttgcaga
     6421 tgactgggcc catttccacg cattctctgg agtcaactgc acccccagtg gggaagaagg
     6481 gaacctcagc tctctctgcc ctgttggaga tggaggccag tcagaagtgc ctgggagaac
     6541 agcaggcagc tgtgcatggt gggaagagct ccgcccagtc agccgagagc agcagcgttg
     6601 ccatgactcc cacctacatg gacagcccac gaaaggacgg ggccttcttt atggagtttg
     6661 tccgcagccc acgcacagca tcgtccgcct tctaccctca ggtatctgtg gaccaaacag
     6721 ccactcctat gttggacggc accagtttgg gcatatgcac aggccaatcc atggacagag
     6781 gcaacagcca gacctttggg aactcccaga acataggaga acagggctac tcctccacaa
     6841 actccagtga cagcagctct cagcagctgg tggcaagctc cttgacctca tcctctaccc
     6901 tgacagagat cctggaagcc atgaagcacc cctcgacagg agtccagctg ctctctgaac
     6961 agaagggcct ctcaccgtac tgcttcatca gcgcggaggt ggtacactgg ttggtgaacc
     7021 acgtggaggg gatccagaca caggcgatgg ccattgacat catgcagaaa atgctggaag
     7081 agcagctcat cacacatgca tctggcgaag cctggcggac cttcatctac ggcttctatt
     7141 tctacaagat agtaacggac aaagagcccg accgagtggc catgcagcag cccgccacca
     7201 cctggcacac agcaggagtg gacgacttcg ccagcttcca gcgcaagtgg tttgaggtgg
     7261 cctttgtggc agaagagctc gtgcactctg agattcctgc ctttctcctg ccctggctgc
     7321 ctagccggcc agcctcctat gcaagtaggc acagctcctt tagccgaagt tttggaggac
     7381 ggagccaggc ggcagcactt ttagctgcca ctgtcccaga gcagaggact gtgaccctgg
     7441 atgttgacgt gaacaaccgc acagaccggc tggagtggtg cagctgttat taccatggca
     7501 acttttctct gaatgcagcc tttgagatca agctgcactg gatggcggtg accgcagcag
     7561 tactcttcga gatggtccaa ggttggcatc ggaaagccac ctcctgtggc ttcttgttag
     7621 tcccagtttt ggaggggcct tttgcactgc ccagttacct gtatggcgac ccccttcgtg
     7681 cccagctctt catcccactc aacatcagct gcttgctcaa ggagggcagc gagcacctgt
     7741 ttgatagctt tgaacccgaa acgtactggg atcgaatgca cctcttccag gaagccattg
     7801 cacacaggtt tgggtttgta caagataaat attctgcctc tgcttttaac ttccctgctg
     7861 agaacaagcc tcagtatatc cacgttacag gaacagtgtt tctgcagctg ccctactcca
     7921 agcgcaagtt ctcagggcag cagcggcggc ggcggaactc caccagctcc accaaccaga
     7981 acatgttctg cgaggagcgg gtcggctaca actgggccta caacaccatg ctcaccaaaa
     8041 catggcgctc cagcgccaca ggggatgaaa agtttgctga tcggctgctg aaggacttca
     8101 cggacttctg catcaaccgt gacaaccggc tggtcacgtt ctggacaagt tgcctggaga
     8161 agatgcatgc cagtgccccg tgagaattct cgacctcgag acaaatggca gtattcatcc
     8221 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     8281 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     8341 ttcgggttta ttacagggac agcagagatc cactttggcc gcggctcgag ggggttgggg
     8401 ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt
     8461 tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc
     8521 gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc
     8581 ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca
     8641 cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg
     8701 cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa
     8761 ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg
     8821 tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga
     8881 atcaccgacc tctctcccca gggggatcca ccggagctta ccatgaccga gtacaagccc
     8941 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     9001 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     9061 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     9121 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     9181 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     9241 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     9301 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     9361 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     9421 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     9481 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg
     9541 cagcgcccga ccgaaaggag cgcacgaccc catgcatcgg tacctttaag accaatgact
     9601 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     9661 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
     9721 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     9781 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     9841 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     9901 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     9961 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
    10021 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
    10081 gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
    10141 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
    10201 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt
    10261 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg
    10321 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
    10381 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
    10441 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
    10501 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
    10561 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
    10621 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
    10681 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
    10741 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
    10801 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
    10861 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc
    10921 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
    10981 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
    11041 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
    11101 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
    11161 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
    11221 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
    11281 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
    11341 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
    11401 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
    11461 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
    11521 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
    11581 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
    11641 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
    11701 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
    11761 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
    11821 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
    11881 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
    11941 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
    12001 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
    12061 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
    12121 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
    12181 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
    12241 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
    12301 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
    12361 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
    12421 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
    12481 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
    12541 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
    12601 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
    12661 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
    12721 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
    12781 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
    12841 gaagagcgcc caatacgcaa accgcctctc cc
//
