LOCUS       sequence-211215-       12872 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lenti-viral expression of Flag-tagged Depdc5 mutant B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12872)
  AUTHORS   Shen K, Huang RK, Brignole EJ, Condon KJ, Valenstein ML, 
            Chantranupong L, Bomaliyamu A, Choe A, Hong C, Yu Z, Sabatini DM
  TITLE     Architecture of the human GATOR1 and GATOR1-Rag GTPases complexes.
  JOURNAL   Nature. 2018 Apr 5;556(7699):64-69. doi: 10.1038/nature26158. Epub 
            2018 Mar 28.
  PUBMED    29590090
REFERENCE   2  (bases 1 to 12872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature26158"; journalName: "Nature"; date: "2018-04-5- 5"; 
            volume: "556"; issue: "7699"; pages: "64-69"
COMMENT     Sequence Label: [pSK481] pLJC5 Flag-Depdc5(B)
FEATURES             Location/Qualifiers
     source          1..12872
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    288..309
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(324..329,330..347,348..354)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    362..378
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     367..389
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     386..402
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     386..402
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     421..441
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        423..441
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        469..695
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             696..876
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    923..1048
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1541..1774
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1959..2003
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2292..3503
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3340..3359
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      3524..3533
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3533..3556
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    8434..8551
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        8600..9110
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     9029..9047
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             9132..9731
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9132..9151)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9628..9648
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             9859..10092
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10164..10285
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10201..10220)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10255..10274
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10325..10460
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10480..10499)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10481..10499)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10509..10526)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10509..10525)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10518..10540)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10667..11122
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10754..10773)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10964..10985
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11148..11252
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11253..11321,11322..12113)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11471..11490)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12284..12872
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12773..12792
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      241 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      301 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      361 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
      421 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg
      481 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
      541 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
      601 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag
      661 atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct
      721 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
      781 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
      841 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
      901 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
      961 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     1021 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     1081 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     1141 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     1201 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     1261 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     1321 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     1381 gcaagcagca gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     1441 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     1501 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     1561 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     1621 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     1681 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     1741 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     1801 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     1861 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     1921 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     1981 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     2041 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     2101 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     2161 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     2221 gagacagatc cattcgatta gtgaacggat ctcgacggta tcgatcacga gactagcctc
     2281 gagcgggatc tggcctccgc gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac
     2341 ggcgagcgct gccacgtcag acgaagggcg cagcgagcgt cctgatcctt ccgcccggac
     2401 gctcaggaca gcggcccgct gctcataaga ctcggcctta gaaccccagt atcagcagaa
     2461 ggacatttta ggacgggact tgggtgactc tagggcactg gttttctttc cagagagcgg
     2521 aacaggcgag gaaaagtagt cccttctcgg cgattctgcg gagggatctc cgtggggcgg
     2581 tgaacgccga tgattatata aggacgcgcc gggtgtggca cagctagttc cgtcgcagcc
     2641 gggatttggg tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg tgagtagcgg
     2701 gctgctgggc tggccggggc tttcgtggcc gccgggccgc tcggtgggac ggaagcgtgt
     2761 ggagagaccg ccaagggctg tagtctgggt ccgcgagcaa ggttgccctg aactgggggt
     2821 tggggggagc gcagcaaaat ggcggctgtt cccgagtctt gaatggaaga cgcttgtgag
     2881 gcgggctgtg aggtcgttga aacaaggtgg ggggcatggt gggcggcaag aacccaaggt
     2941 cttgaggcct tcgctaatgc gggaaagctc ttattcgggt gagatgggct ggggcaccat
     3001 ctggggaccc tgacgtgaag tttgtcactg actggagaac tcggtttgtc gtctgttgcg
     3061 ggggcggcag ttatggcggt gccgttgggc agtgcacccg tacctttggg agcgcgcgcc
     3121 ctcgtcgtgt cgtgacgtca cccgttctgt tggcttataa tgcagggtgg ggccacctgc
     3181 cggtaggtgt gcggtaggct tttctccgtc gcaggacgca gggttcgggc ctagggtagg
     3241 ctctcctgaa tcgacaggcg ccggacctct ggtgagggga gggataagtg aggcgtcagt
     3301 ttctttggtc ggttttatgt acctatcttc ttaagtagct gaagctccgg ttttgaacta
     3361 tgcgctcggg gttggcgagt gtgttttgtg aagtttttta ggcacctttt gaaatgtaat
     3421 catttgggtc aatatgtaat tttcagtgtt agactagtaa attgtccgct aaattctggc
     3481 cgtttttggc ttttttgtta gacgaagcgc tagcgctacc ggtgccacca tggactacaa
     3541 ggatgacgat gacaagggtg gtggtggtgg tgcgtcgacg atgagaacaa caaaggtcta
     3601 caaactcgtc atccacaaga agggctttgg gggcagtgat gatgagctag ttgtgaaccc
     3661 caaagtgttc cctcacatca agcttggaga cattgtagag attgcacacc ccaacgatga
     3721 atacagccct ctgcttttgc aggtcaagtc tcttaaggaa gatttacaga aggaaactat
     3781 cagtgtggac cagactgtga ctcaagtgtt ccggctgaga ccttatcagg atgtctatgt
     3841 taatgtcgta gaccctaagg atgtgaccct tgacctagtg gaattaactt ttaaggatca
     3901 gtatattggc cgtggggata tgtggcgact aaagaaaagt ttggtcagca catgtgccta
     3961 tatcacccag aaggtggagt ttgctggcat cagagcacag gctggtgaac tgtgggttaa
     4021 gaatgagaag gtcatgtgtg gctacatcag tgaagatacc agggtggtgt ttcgttctac
     4081 gtcggctatg gtttacatat ttattcagat gagctgtgaa atgtgggatt ttgatattta
     4141 tggggatttg tattttgaga aagctgtgaa tggtttcctt gctgatctat ttaccaagtg
     4201 gaaggagaag aactgtagtc atgaagtgac agtggtcctg ttttctagaa ctttctatga
     4261 tgcaaaatct gttgatgaat ttcctgaaat aaaccgagcc tcaattcgac aggatcataa
     4321 ggggagattc tatgaagact tttacaaagt ggtggtgcag aatgagagaa gagaagaatg
     4381 gacttcactt ctcgtaacca ttaaaaaact cttcatccag tatccagtgt tggtgcgact
     4441 ggaacaggca gagggctttc ctcaaggaga taattctacc tcagcacaag gaaactacct
     4501 ggaggccatc aatctgtcat tcaatgtgtt tgataagcac tacatcaacc gcaactttga
     4561 ccgaactggg cagatgtcag tggtgatcac gcccggggtg ggtgtctttg aagtggaccg
     4621 cctactcatg atcctgacca agcagcggat gatagataat ggaattggtg tggatttggt
     4681 gtgcatggga ggcagcggaa gcggcgctgt cccattgttc aagctccata atcggagtgc
     4741 tccccgtgat tctcgtctgg gcgatgacta taatatccct cactggataa accacagttt
     4801 ctacacatcc aaaagccagc tcttttgtaa tagtttcacc ccacgaataa aactggcagg
     4861 aaagaagccc gcctctgaga aagcaaaaaa tggccgtgat acatctctcg ggagtccaaa
     4921 agaatctgag aacgcccttc ccatccaagt agattatgac gcctatgacg ctcaagtgtt
     4981 caggctgccc ggcccatccc gggcccagtg cctcaccacc tgcagatctg tgcgagagcg
     5041 agagagtcac agtcgaaaga gtgccagctc ctgtgatgtt tcatccagcc cttccctacc
     5101 aagccgcaca ctgcccactg aggaagtgag gagccaggct tctgacgaca gctccctagg
     5161 caagagtgcc aacatcctga tgatcccaca cccccacctg caccagtatg aagtcagcag
     5221 ctccttggga tacaccagca ctcgagatgt cctggagaac atgatggagc caccacagcg
     5281 agactccagt gcaccaggga ggtttcacgt tggcagtgca gaatccatgc tgcatgttcg
     5341 acctggtgga tacacgcccc agagagcact gattaacccc ttcgctccct ctcggatgcc
     5401 catgaagctt acgtccaaca gaaggcgctg gatgcacact tttcctgtgg ggccatccgg
     5461 agaagccatc cagatccacc accagacccg acagaatatg gcggagctac aaggcagcgg
     5521 gcagagggat ccaactcact cctctgcaga gctgctggag ttagcatatc atgaagctgc
     5581 tggaaggcac agcaattccc gccagcctgg tgacggcatg tccttcttga acttcagtgg
     5641 aacagaggag ctttctgtcg gcctgcttag caacagtggt gcaggtatga atcctaggac
     5701 ccagaataag gattctctag aggacagtgt ttctacctct ccagacccaa ttctgacact
     5761 gtctgctccc cctgtagtgc caggcttctg ttgcacagtt ggagtggact ggaagtctct
     5821 cactactccg gcgtgcctcc cccttaccac cgactacttc cctgaccgcc agggcctgca
     5881 gaatgactac acagagggct gttatgatct ccttccagaa gcagacatcg acaggaggga
     5941 cgaagatggt gtgcagatga cagcccagca ggtatttgaa gagtttattt gccaacgtct
     6001 catgcagggc taccaaatca tagtgcagcc caagacacag aaacccaatc ctgctgtccc
     6061 gcccccgctg agcagtagcc cactctatag ccgaggcctt gtgtcccgaa accgccctga
     6121 ggaggaggac cagtattggc tgagtatggg cagaacgttc cacaaagtga cgctgaagga
     6181 taagatgatc acagtgacgc gataccttcc caagtatcct tatgaatctg cccagatcca
     6241 ctacacctac agcctctgtc cttcccactc agactcagag ttcgtctcct gctgggtgga
     6301 gttttcccac gaacggctgg aggagtacaa gtggaattac ttagatcagt atatctgttc
     6361 tgccggctct gaagacttca gcttaattga gtccctgaag ttctggagga cccgcttcct
     6421 gctgctgcca gcctgtgtca ccgccaccaa gcgcatcacg gagggggagg cccactgcga
     6481 catctatggg gacaggcccc gtgcagacga ggacgagtgg caactcctgg atggttttgt
     6541 ccgctttgtg gagggcttga atcgcattcg caggcggcat cgctcggatc gcatgatgcg
     6601 gaaagggacc gccatgaaag gcttgcagat gactgggccc atttccacgc attctctgga
     6661 gtcaactgca cccccagtgg ggaagaaggg aacctcagct ctctctgccc tgttggagat
     6721 ggaggccagt cagaagtgcc tgggagaaca gcaggcagct gtgcatggtg ggaagagctc
     6781 cgcccagtca gccgagagca gcagcgttgc catgactccc acctacatgg acagcccacg
     6841 aaaggacggg gccttcttta tggagtttgt ccgcagccca cgcacagcat cgtccgcctt
     6901 ctaccctcag gtatctgtgg accaaacagc cactcctatg ttggacggca ccagtttggg
     6961 catatgcaca ggccaatcca tggacagagg caacagccag acctttggga actcccagaa
     7021 cataggagaa cagggctact cctccacaaa ctccagtgac agcagctctc agcagctggt
     7081 ggcaagctcc ttgacctcat cctctaccct gacagagatc ctggaagcca tgaagcaccc
     7141 ctcgacagga gtccagctgc tctctgaaca gaagggcctc tcaccgtact gcttcatcag
     7201 cgcggaggtg gtacactggt tggtgaacca cgtggagggg atccagacac aggcgatggc
     7261 cattgacatc atgcagaaaa tgctggaaga gcagctcatc acacatgcat ctggcgaagc
     7321 ctggcggacc ttcatctacg gcttctattt ctacaagata gtaacggaca aagagcccga
     7381 ccgagtggcc atgcagcagc ccgccaccac ctggcacaca gcaggagtgg acgacttcgc
     7441 cagcttccag cgcaagtggt ttgaggtggc ctttgtggca gaagagctcg tgcactctga
     7501 gattcctgcc tttctcctgc cctggctgcc tagccggcca gcctcctatg caagtaggca
     7561 cagctccttt agccgaagtt ttggaggacg gagccaggcg gcagcacttt tagctgccac
     7621 tgtcccagag cagaggactg tgaccctgga tgttgacgtg aacaaccgca cagaccggct
     7681 ggagtggtgc agctgttatt accatggcaa cttttctctg aatgcagcct ttgagatcaa
     7741 gctgcactgg atggcggtga ccgcagcagt actcttcgag atggtccaag gttggcatcg
     7801 gaaagccacc tcctgtggct tcttgttagt cccagttttg gaggggcctt ttgcactgcc
     7861 cagttacctg tatggcgacc cccttcgtgc ccagctcttc atcccactca acatcagctg
     7921 cttgctcaag gagggcagcg agcacctgtt tgatagcttt gaacccgaaa cgtactggga
     7981 tcgaatgcac ctcttccagg aagccattgc acacaggttt gggtttgtac aagataaata
     8041 ttctgcctct gcttttaact tccctgctga gaacaagcct cagtatatcc acgttacagg
     8101 aacagtgttt ctgcagctgc cctactccaa gcgcaagttc tcagggcagc agcggcggcg
     8161 gcggaactcc accagctcca ccaaccagaa catgttctgc gaggagcggg tcggctacaa
     8221 ctgggcctac aacaccatgc tcaccaaaac atggcgctcc agcgccacag gggatgaaaa
     8281 gtttgctgat cggctgctga aggacttcac ggacttctgc atcaaccgtg acaaccggct
     8341 ggtcacgttc tggacaagtt gcctggagaa gatgcatgcc agtgccccgt gagaattctc
     8401 gacctcgaga caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
     8461 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     8521 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
     8581 actttggccg cggctcgagg gggttggggt tgcgcctttt ccaaggcagc cctgggtttg
     8641 cgcagggacg cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt
     8701 ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat cttcgccgct acccttgtgg
     8761 gccccccggc gacgcttcct gctccgcccc taagtcggga aggttccttg cggttcgcgg
     8821 cgtgccggac gtgacaaacg gaagccgcac gtctcactag taccctcgca gacggacagc
     8881 gccagggagc aatggcagcg cgccgaccgc gatgggctgt ggccaatagc ggctgctcag
     8941 cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg
     9001 tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca
     9061 cgtcggcagt cggctccctc gttgaccgaa tcaccgacct ctctccccag ggggatccac
     9121 cggagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     9181 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     9241 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     9301 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     9361 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     9421 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     9481 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     9541 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     9601 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     9661 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     9721 ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc
     9781 atgcatcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     9841 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     9901 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     9961 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
    10021 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
    10081 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
    10141 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
    10201 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
    10261 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
    10321 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
    10381 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
    10441 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac
    10501 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
    10561 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
    10621 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
    10681 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
    10741 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
    10801 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
    10861 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
    10921 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
    10981 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
    11041 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
    11101 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
    11161 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
    11221 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
    11281 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
    11341 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
    11401 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
    11461 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
    11521 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
    11581 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
    11641 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
    11701 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
    11761 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
    11821 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
    11881 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
    11941 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
    12001 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
    12061 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
    12121 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
    12181 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
    12241 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
    12301 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
    12361 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
    12421 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
    12481 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
    12541 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
    12601 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
    12661 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
    12721 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
    12781 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
    12841 gatgctcgtc aggggggcgg agcctatgga aa
//
