LOCUS       sequence-211240-        1794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Archaerhodopsin fused to mRuby2 and targeted to the neuronal soma 
            and proximal dendrites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Mardinly AR, Oldenburg IA, Pegard NC, Sridharan S, Lyall EH, Chesnov
            K, Brohawn SG, Waller L, Adesnik H
  TITLE     Precise multimodal optical control of neural ensemble activity.
  JOURNAL   Nat Neurosci. 2018 Apr 30. pii: 10.1038/s41593-018-0139-8. doi: 
            10.1038/s41593-018-0139-8.
  PUBMED    29713079
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2018 Apr 30. pii: 10.1038/s41593-018-0139-8. doi: 
            10.1038/s41593-018-0139-8."
COMMENT     Sequence Label: pCAG-eArch3.0-mRuby2-ST
FEATURES             Location/Qualifiers
     source          1..1794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..774
                     /codon_start=1
                     /product="light-driven proton pump archaerhodopsin-3 from 
                     Halorubrum sodomense (Chow et al., 2010)"
                     /label=Arch
                     /translation="MDPIALQAGYDLLGDGRPETLWLGIGTLLMLIGTFYFLVRGWGVT
                     DKDAREYYAVTILVPGIASAAYLSMFFGIGLTEVTVGGEMLDIYYARYADWLFTTPLLL
                     LDLALLAKVDRVTIGTLVGVDALMIVTGLIGALSHTAIARYSWWLFSTICMIVVLYFLA
                     TSLRSAAKERGPEVASTFNTLTALVLVLWTAYPILWIIGTEGAGVVGLGIETLLFMVLD
                     VTAKVGFGFILLRSRAILGDTEAPEPSAGADVSAAD"
     CDS             856..1566
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 atggacccca tcgctctgca ggctggttac gacctgctgg gtgacggcag acctgaaact
       61 ctgtggctgg gcatcggcac tctgctgatg ctgattggaa ccttctactt tctggtccgc
      121 ggatggggag tcaccgataa ggatgcccgg gaatattacg ctgtgactat cctggtgccc
      181 ggaatcgcat ccgccgcata tctgtctatg ttctttggta tcgggcttac tgaggtgacc
      241 gtcgggggcg aaatgttgga tatctattat gccaggtacg ccgactggct gtttaccacc
      301 ccacttctgc tgctggatct ggcccttctc gctaaggtgg atcgggtgac catcggcacc
      361 ctggtgggtg tggacgccct gatgatcgtc actggcctca tcggagcctt gagccacacg
      421 gccatagcca gatacagttg gtggttgttc tctacaattt gcatgatagt ggtgctctat
      481 tttctggcta catccctgcg atctgctgca aaggagcggg gccccgaggt ggcatctacc
      541 tttaacaccc tgacagctct ggtcttggtg ctgtggaccg cttaccctat cctgtggatc
      601 ataggcactg agggcgctgg cgtggtgggc ctgggcatcg aaactctgct gtttatggtg
      661 ttggacgtga ctgccaaggt cggctttggc tttatcctgt tgagatcccg ggctattctg
      721 ggcgacaccg aggcaccaga acccagtgcc ggtgccgatg tcagtgccgc cgacctcgag
      781 aagagcagga tcaccagcga gggcgagtac atccccctgg accagatcga catcaacgtg
      841 gcggccgcac cggtaatggt gtctaagggc gaagagctga tcaaggaaaa tatgcgtatg
      901 aaggtggtca tggaaggttc ggtcaacggc caccaattca aatgcacagg tgaaggagaa
      961 ggcaatccgt acatgggaac tcaaaccatg aggatcaaag tcatcgaggg aggacccctg
     1021 ccatttgcct ttgacattct tgccacgtcg ttcatgtatg gcagccgtac ttttatcaag
     1081 tacccgaaag gcattcctga tttctttaaa cagtcctttc ctgagggttt tacttgggaa
     1141 agagttacga gatacgaaga tggtggagtc gtcaccgtca tgcaggacac cagccttgag
     1201 gatggctgtc tcgtttacca cgtccaagtc agaggggtaa actttccctc caatggtccc
     1261 gtgatgcaga agaagaccaa gggttgggag cctaatacag agatgatgta tccagcagat
     1321 ggtggtctga ggggatacac tcatatggca ctgaaagttg atggtggtgg ccatctgtct
     1381 tgctctttcg taacaactta caggtcaaaa aagaccgtcg ggaacatcaa gatgcccggt
     1441 atccatgccg ttgatcaccg cctggaaagg ttagaggaaa gtgacaatga aatgttcgta
     1501 gtacaacgcg aacacgcagt tgccaagttc gccgggcttg gtggtgggat ggacgagctg
     1561 tacaaggccc tagagcagtc ccagcccatc ctcaacacca aggagatggc cccgcagagc
     1621 aagcctccag aggagctgga gatgagcagc atgcccagcc ccgtggcccc tctgcccgca
     1681 cgcacggagg gcgtcatcga catgcggagc atgtccagca ttgacagctt catcagctgt
     1741 gccacggact tccctgaagc caccagattc ttctgctacg agaacgaggt gtaa
//
