LOCUS       sequence-211243-        1950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Artificial anion channelrhodopsin iC++ fused to ER export signal and
            fluorophore mRuby2 and targeted to the neuronal soma and proximal 
            dendrites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Mardinly AR, Oldenburg IA, Pegard NC, Sridharan S, Lyall EH, Chesnov
            K, Brohawn SG, Waller L, Adesnik H
  TITLE     Precise multimodal optical control of neural ensemble activity.
  JOURNAL   Nat Neurosci. 2018 Apr 30. pii: 10.1038/s41593-018-0139-8. doi: 
            10.1038/s41593-018-0139-8.
  PUBMED    29713079
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2018 Apr 30. pii: 10.1038/s41593-018-0139-8. doi: 
            10.1038/s41593-018-0139-8."
COMMENT     Sequence Label: pCAG-eiC++-mRuby2-ST
FEATURES             Location/Qualifiers
     source          1..1950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1012..1722
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 atggactatg gcggcgcttt gtctgccgtc ggactcttcc aaacctcata cactcttgag
       61 aacaatggtt ctgttatttg catcccgaac aacggccagt gcttctgctt ggcttggctt
      121 aaatccaacg gaacaaatgc cgagaagttg gctgccaaca ttctgcagtg gatttctttt
      181 gcgctttcag cgctctgcct gatgttctac ggctaccaga cctggaagtc tacttgcggc
      241 tgggagaata tttacgtggc cacgatccag atgatcaagt tcatcatcga gtatttccat
      301 agctttgacg aacctgcggt gatctactca tccaacggca acaagaccag gtggcttcgt
      361 tacgcgagct ggctgctgac ctgccctgtc attcttatcc acctgagcaa ccttacgggt
      421 ctggcgaacg actataacaa gcgtaccatg ggtctgctgg tgtcagatat cggcacgatc
      481 gtgtggggca ccacggccgc gctgtccaag ggatacgtcc gtgtcatttt cttcctgatg
      541 ggcctgtgct acggcatcta cacattcttc aacgcagcca aggtctacat tgaggcgtac
      601 cacaccgtgc cgaagggccg gtgtcgccag gtggtgactg gcatggcttg gctcttcttc
      661 gtatcatggg gtatgttccc catcctgttc atcctcggcc ccgagggctt cggcgtcctg
      721 agcaggtacg gctccaacgt cggccacacc atcattgacc tgatgtcgaa gcagtgctgg
      781 ggtctgctcg gccactacct gcgcgtgctg atccacagcc atatcctcat ccacggcgac
      841 attcgcaaga ccaccaaatt gaacattggt ggcactgaga ttgaggtcga gacgctggtg
      901 gaggacgagg ccgaggctgg cgcggtcgcg gccgccaaga gcaggatcac cagcgagggc
      961 gagtacatcc ccctggacca gatcgacatc aacgtggcgg cggcaccggt aatggtgtct
     1021 aagggcgaag agctgatcaa ggaaaatatg cgtatgaagg tggtcatgga aggttcggtc
     1081 aacggccacc aattcaaatg cacaggtgaa ggagaaggca atccgtacat gggaactcaa
     1141 accatgagga tcaaagtcat cgagggagga cccctgccat ttgcctttga cattcttgcc
     1201 acgtcgttca tgtatggcag ccgtactttt atcaagtacc cgaaaggcat tcctgatttc
     1261 tttaaacagt cctttcctga gggttttact tgggaaagag ttacgagata cgaagatggt
     1321 ggagtcgtca ccgtcatgca ggacaccagc cttgaggatg gctgtctcgt ttaccacgtc
     1381 caagtcagag gggtaaactt tccctccaat ggtcccgtga tgcagaagaa gaccaagggt
     1441 tgggagccta atacagagat gatgtatcca gcagatggtg gtctgagggg atacactcat
     1501 atggcactga aagttgatgg tggtggccat ctgtcttgct ctttcgtaac aacttacagg
     1561 tcaaaaaaga ccgtcgggaa catcaagatg cccggtatcc atgccgttga tcaccgcctg
     1621 gaaaggttag aggaaagtga caatgaaatg ttcgtagtac aacgcgaaca cgcagttgcc
     1681 aagttcgccg ggcttggtgg tgggatggac gagctgtaca aggccctaga gcagtcccag
     1741 cccatcctca acaccaagga gatggccccg cagagcaagc ctccagagga gctggagatg
     1801 agcagcatgc ccagccccgt ggcccctctg cccgcacgca cggagggcgt catcgacatg
     1861 cggagcatgt ccagcattga cagcttcatc agctgtgcca cggacttccc tgaagccacc
     1921 agattcttct gctacgagaa cgaggtgtaa
//
