LOCUS       sequence-211244-        1797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cation channelrhodopsin CoChR fused to mRuby2 fluorophore and 
            targeted to the neuronal soma and proximal dendrites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Mardinly AR, Oldenburg IA, Pegard NC, Sridharan S, Lyall EH, Chesnov
            K, Brohawn SG, Waller L, Adesnik H
  TITLE     Precise multimodal optical control of neural ensemble activity.
  JOURNAL   Nat Neurosci. 2018 Apr 30. pii: 10.1038/s41593-018-0139-8. doi: 
            10.1038/s41593-018-0139-8.
  PUBMED    29713079
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2018 Apr 30. pii: 10.1038/s41593-018-0139-8. doi: 
            10.1038/s41593-018-0139-8."
COMMENT     Sequence Label: pCAG-CoChR-mRuby2-ST
FEATURES             Location/Qualifiers
     source          1..1797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..864
                     /codon_start=1
                     /product="high-photocurrent channelrhodopsin from 
                     Chloromonas oogama (Shemesh et al., 2017)"
                     /label=CoChR
                     /translation="MLGNGSAIVPIDQCFCLAWTDSLGSDTEQLVANILQWFAFGFSIL
                     ILMFYAYQTWRATCGWEEVYVCCVELTKVIIEFFHEFDDPSMLYLANGHRVQWLRYAEW
                     LLTCPVILIHLSNLTGLKDDYSKRTMRLLVSDVGTIVWGATSAMSTGYVKVIFFVLGCI
                     YGANTFFHAAKVYIESYHVVPKGRPRTVVRIMAWLFFLSWGMFPVLFVVGPEGFDAISV
                     YGSTIGHTIIDLMSKNCWGLLGHYLRVLIHQHIIIYGDIRKKTKINVAGEEMEVETMVD
                     QEDEETV"
     CDS             880..1590
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 atgctgggaa acggcagcgc cattgtgcct atcgaccagt gcttttgcct ggcttggacc
       61 gacagcctgg gaagcgatac agagcagctg gtggccaaca tcctccagtg gttcgccttc
      121 ggcttcagca tcctgatcct gatgttctac gcctaccaga cttggagagc cacttgcggt
      181 tgggaggagg tctacgtctg ttgcgtcgag ctgaccaagg tcatcatcga gttcttccac
      241 gagttcgacg accccagcat gctgtacctg gctaacggac accgagtcca gtggctgaga
      301 tacgcagagt ggctgctgac ttgtcccgtc atcctgatcc acctgagcaa cctgaccggc
      361 ctgaaggacg actacagcaa gcggaccatg aggctgctgg tgtcagacgt gggaaccatc
      421 gtgtggggag ctacaagcgc catgagcaca ggctacgtca aggtcatctt cttcgtgctg
      481 ggttgcatct acggcgccaa caccttcttc cacgccgcca aggtgtatat cgagagctac
      541 cacgtggtgc caaagggcag acctagaacc gtcgtgcgga tcatggcttg gctgttcttc
      601 ctgtcttggg gcatgttccc cgtgctgttc gtcgtgggac cagaaggatt cgacgccatc
      661 agcgtgtacg gctctaccat tggccacacc atcatcgacc tcatgagcaa gaattgttgg
      721 ggcctgctgg gacactatct gagagtgctg atccaccagc acatcatcat ctacggcgac
      781 atccggaaga agaccaagat caacgtggcc ggcgaggaga tggaagtgga gaccatggtg
      841 gaccaggagg acgaggagac agtggcggcc gcaccggtaa tggtgtctaa gggcgaagag
      901 ctgatcaagg aaaatatgcg tatgaaggtg gtcatggaag gttcggtcaa cggccaccaa
      961 ttcaaatgca caggtgaagg agaaggcaat ccgtacatgg gaactcaaac catgaggatc
     1021 aaagtcatcg agggaggacc cctgccattt gcctttgaca ttcttgccac gtcgttcatg
     1081 tatggcagcc gtacttttat caagtacccg aaaggcattc ctgatttctt taaacagtcc
     1141 tttcctgagg gttttacttg ggaaagagtt acgagatacg aagatggtgg agtcgtcacc
     1201 gtcatgcagg acaccagcct tgaggatggc tgtctcgttt accacgtcca agtcagaggg
     1261 gtaaactttc cctccaatgg tcccgtgatg cagaagaaga ccaagggttg ggagcctaat
     1321 acagagatga tgtatccagc agatggtggt ctgaggggat acactcatat ggcactgaaa
     1381 gttgatggtg gtggccatct gtcttgctct ttcgtaacaa cttacaggtc aaaaaagacc
     1441 gtcgggaaca tcaagatgcc cggtatccat gccgttgatc accgcctgga aaggttagag
     1501 gaaagtgaca atgaaatgtt cgtagtacaa cgcgaacacg cagttgccaa gttcgccggg
     1561 cttggtggtg ggatggacga gctgtacaag gccctagagc agtcccagcc catcctcaac
     1621 accaaggaga tggccccgca gagcaagcct ccagaggagc tggagatgag cagcatgccc
     1681 agccccgtgg cccctctgcc cgcacgcacg gagggcgtca tcgacatgcg gagcatgtcc
     1741 agcattgaca gcttcatcag ctgtgccacg gacttccctg aagccaccag attctaa
//
