LOCUS       sequence-211249-        2034 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cation channelrhodopsin C1V1 fused to mRuby2 fluorophore and 
            targeted to the neuronal soma and proximal dendrites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Mardinly AR, Oldenburg IA, Pegard NC, Sridharan S, Lyall EH, Chesnov
            K, Brohawn SG, Waller L, Adesnik H
  TITLE     Precise multimodal optical control of neural ensemble activity.
  JOURNAL   Nat Neurosci. 2018 Apr 30. pii: 10.1038/s41593-018-0139-8. doi: 
            10.1038/s41593-018-0139-8.
  PUBMED    29713079
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2018 Apr 30. pii: 10.1038/s41593-018-0139-8. doi: 
            10.1038/s41593-018-0139-8."
COMMENT     Sequence Label: pCAG-C1V1-TT-mRuby2-ST
FEATURES             Location/Qualifiers
     source          1..2034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1117..1827
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 atgtcgcgga ggccatggct tcttgcccta gcgctggcag tggcgctggc ggccggcagc
       61 gcaggagcct cgactggcag tgacgcgacg gtgccggtcg cgactcagga tggccccgac
      121 tacgttttcc accgtgccca cgagcgcatg ctcttccaaa cctcatacac tcttgagaac
      181 aatggttctg ttatttgcat cccgaacaac ggccagtgct tctgcttggc ttggcttaaa
      241 tccaacggaa caaatgccga gaagttggct gccaacattc tgcagtggat tacttttgcg
      301 ctttcagcgc tctgcctgat gttctacggc taccagacct ggaagtctac ttgcggctgg
      361 gagaccattt acgtggccac gatcgagatg atcaagttca tcatcgagta tttccatgag
      421 tttgacgaac ctgcggtgat ctactcatcc aacggcaaca agaccgtgtg gcttcgttac
      481 gcgacctggc tgctcacctg cccggtcctt ctgatccatc tgagcaacct cacaggcctg
      541 aaggacgatt atagcaaaag gactatgggc ctgttggttt ctgatgtggg atgcatcgtg
      601 tggggcgcaa ccagcgccat gtgtacgggg tggacgaaga tcctgttctt cctcatctca
      661 ttgagctatg gtatgtatac ctattttcat gctgctaaag tttatatcga agcattccac
      721 acagttccaa aagggatttg tcgagaactg gtccgagtga tggcctggac attctttgtg
      781 gcttggggaa tgtttccagt cctgtttctg ctgggcacgg aaggattcgg tcatatcagc
      841 ccttatggat ctgccattgg gcactccatc ctcgacctga ttgcaaagaa catgtggggt
      901 gtgctgggga attacctgcg cgtcaaaatc cacgagcaca tcctgttgta tggcgacatc
      961 agaaagaagc agaaaattac gatcgccggc caagagatgg aggttgagac actggtggct
     1021 gaagaggagg acgcggccgc caagagcagg atcaccagcg agggcgagta catccccctg
     1081 gaccagatcg acatcaacgt ggcggccgca ccggtaatgg tgtctaaggg cgaagagctg
     1141 atcaaggaaa atatgcgtat gaaggtggtc atggaaggtt cggtcaacgg ccaccaattc
     1201 aaatgcacag gtgaaggaga aggcaatccg tacatgggaa ctcaaaccat gaggatcaaa
     1261 gtcatcgagg gaggacccct gccatttgcc tttgacattc ttgccacgtc gttcatgtat
     1321 ggcagccgta cttttatcaa gtacccgaaa ggcattcctg atttctttaa acagtccttt
     1381 cctgagggtt ttacttggga aagagttacg agatacgaag atggtggagt cgtcaccgtc
     1441 atgcaggaca ccagccttga ggatggctgt ctcgtttacc acgtccaagt cagaggggta
     1501 aactttccct ccaatggtcc cgtgatgcag aagaagacca agggttggga gcctaataca
     1561 gagatgatgt atccagcaga tggtggtctg aggggataca ctcatatggc actgaaagtt
     1621 gatggtggtg gccatctgtc ttgctctttc gtaacaactt acaggtcaaa aaagaccgtc
     1681 gggaacatca agatgcccgg tatccatgcc gttgatcacc gcctggaaag gttagaggaa
     1741 agtgacaatg aaatgttcgt agtacaacgc gaacacgcag ttgccaagtt cgccgggctt
     1801 ggtggtggga tggacgagct gtacaaggcc ctagagcagt cccagcccat cctcaacacc
     1861 aaggagatgg ccccgcagag caagcctcca gaggagctgg agatgagcag catgcccagc
     1921 cccgtggccc ctctgcccgc acgcacggag ggcgtcatcg acatgcggag catgtccagc
     1981 attgacagct tcatcagctg tgccacggac ttccctgaag ccaccagatt ctaa
//
